opportunities as preclinical model systems
Báo cáo khoa học: The influence of cold shock proteins on transcription and translation studied in cell-free model systems pdf
Ngày tải lên :
07/03/2014, 21:20
... was stained with Coomassie Brilliant Blue (A) Molecular mass standard; (B) blank; (C) pK7Ras; (D) pET14bRascÂ; (E) pET14bRas The arrow displays the prominent band of H-Ras expressed from pK7ras ... transcription As template DNA the plasmid pK7CAT [36], pET14b CAT, pKRAS and pET14bRas and pET14bRas c were used The plasmids were puried from E coli TG1 using the QIAlter Plasmid Maxi Kit ... transcription translation experiment was used omitting plasmid and T7 RNA polymerase as the components for transcription Instead of these, mRNA was used as template for the translation With this...
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Silicon as a model ion trap: Time domain measurements of donor Rydberg states
Ngày tải lên :
16/03/2014, 15:30
... recombination of free electrons and ions in ˙ the case that Pg Ϸ Px, even if fast In the case of asymmetric recombination, a fast initial transient is expected, whereas the **Eqs can be solved to give ng͑t͒ ... cmϪ1, which was obtained in an isotopically pure 28Si sample Fig shows the absorption spectrum and lifetimes for P-doped Si and As- doped Si samples The absorption spectrum was measured with a ... between 34 and 45 meV are apparent No pump-probe effect was seen when the laser was not resonant The relaxation lifetime of the 2p0 state in Si:P has the longest lifetime because it is farthest from...
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Báo cáo khoa học: Hematopoietic differentiation from human ESCs as a model for developmental studies and future clinical translations Invited review following the FEBS Anniversary Prize received on 5 July 2009 at the 34th FEBS Congress in Prague docx
Ngày tải lên :
22/03/2014, 17:20
... hypothesized, a parent population known as hemangioblasts [38,39] Using the differentiation of mouse ESC as an in vitro model, Keller’s group isolated blast colony-forming cells that were shown ... into a humanized mouse model of sickle cell anemia carrying the human mutant variant of the b-globin gene (bS) that is responsible for the disease Fibroblasts from a diseased animal were reprogrammed ... existence of the hemangioblast in vivo is widely accepted and has been described in Drosophila, zebrafish and mice [41–43], identification of a human hemangioblast in vivo has not yet been achieved,...
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Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
Ngày tải lên :
24/03/2014, 00:21
... The reaction was initiated by the addition of lg enzyme + Three activities of Na /K -ATPase were determined as follows Na+/K+-ATPase activity was measured by monitoring the decrease in NADH absorbance ... kinase, and 2.5 lgámL)1 LDH in a total volume of mL The reaction was initiated by an addition of 50 ng of Na+/K+-ATPase Na+-dependent ATPase activity was also measured using the coupled enzyme assay ... membrane-Na+/K+-ATPase Tritium-bound Na+/K+-ATPase was prepared by pressurization at 200 MPa for 15 as described in Fig and digested with trypsin Bound tritium was measured during tryptic digestion as described...
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Báo cáo sinh học: "An NIH intramural percubator as a model of academic-industry partnerships: from the beginning of life through the valley of death" pptx
Ngày tải lên :
18/06/2014, 19:20
... distinguished past is an equally distinguished future Competing interests MRE-B is a translational researcher who has participated in many laboratory and clinical research studies, as well as several ... to the needs of the market, which in turn will aid in assessing the translational potential of ideas that are still in the percolation phase The NIH intramural program is an ideal test site for ... And as a practical matter, designating percubator investigators and their companies as ‘contractors and contract companies’ would be a simple route to initiating a pilot program in Bethesda as...
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Báo cáo sinh học: " The Israeli strain IS-98-ST1 of West Nile virus as viral model for West Nile encephalitis in the Old World" pdf
Ngày tải lên :
18/06/2014, 22:20
... 2004, 1:9 by the fact that an Israeli WNV strain was introduced in New York City in 1999 [4] The murine model of WNV-associated encephalitis has been widely used to address the viral pathogenesis[5] ... accession number AF481864) Virus titration was performed on AP61 cells by focus immunodetection assay as previously described [9] Infectivity titers were expressed as focus forming units (FFU) In this ... mice were assessed for the presence of viral antigens by immunofluorescence at day p.i When inoculated i.c, virus was found widespread in most of the brain structures (whereas no signal was seen...
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báo cáo hóa học:" An NIH intramural percubator as a model of academic-industry partnerships: from the beginning of life through the valley of death" ppt
Ngày tải lên :
20/06/2014, 03:20
... distinguished past is an equally distinguished future Competing interests MRE-B is a translational researcher who has participated in many laboratory and clinical research studies, as well as several ... to the needs of the market, which in turn will aid in assessing the translational potential of ideas that are still in the percolation phase The NIH intramural program is an ideal test site for ... And as a practical matter, designating percubator investigators and their companies as ‘contractors and contract companies’ would be a simple route to initiating a pilot program in Bethesda as...
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Biofuels, Solar and Wind as Renewable Energy Systems: Benefits and Risks pptx
Ngày tải lên :
27/06/2014, 08:20
... impacts of burning biomass are less harmful than those associated with coal, but more harmful than those associated with natural gas (Pimentel 2001) Biomass combustion releases more than 200 different ... be harvested and utilized as a fuel source This would be a disaster for agricultural ecosystems Without the protection of crop residues, soil loss may increase as much as 100-fold (Fryrear and ... each year and quantified as mass of carbon used to build stems, leaves and roots Note that in the large portions of South and East Asia, Western Europe, Middle East, and eastern US, humans grab...
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Cell Membrane: The Red Blood Cell as a Model ppt
Ngày tải lên :
29/06/2014, 11:20
... Masahiro Yoshimoto, Kazuyuki Mitani, Kosuke Miyashima, Masakiyo Mannoji, Takashi Sugihara, Nobumasa Inoue, Masaoo Shimoda, Akio Kanzaki, Masashi Hashimoto, Hiroo Mori, Takemi Otsuki, Kazuyuki ... 4.1 was rigorously investigated by Conboy, Chasis, Takakuwa, and others along with Mohandas Protein 4.2 was first recognized by its deficiency, which was discovered independently by Hayashi et ... phospholipids Increased PC Increased FC and SM Saturation of fatty acids Increased unsaturation Increased saturation Length of acyl chains Increased shorter chains (C : 14Z16) Increased longer chains...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 2 Part 11 doc
Ngày tải lên :
06/08/2014, 04:20
... regarded as even part of the answer when, as we have seen, the growth of biofuels could not match the growth in U.S population Insofar as that attempt is made, it will continue to increase the ... ‘energy fantasists’ assume there are boundless hectares of potential sugarcane land, we have taken Brazil as an example, but even with a high yield of 88 tonnes of sugarcane per hectare, as might ... 446 GJ/ha/yr = 14 kW/ha The figure is easy to cross-check, as 88,000 kg at 70% moisture content would contain 26,400 kg of dry matter, and as dry matter has an energy content in the region of...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 1 ppt
Ngày tải lên :
06/08/2014, 04:20
... impacts of burning biomass are less harmful than those associated with coal, but more harmful than those associated with natural gas (Pimentel 2001) Biomass combustion releases more than 200 different ... its energy in the future: hydropower, biomass, wind power, solar thermal, photovoltaics, passive energy systems, geothermal, and biogas In this chapter, I assess the potential of these renewable ... its potential, as well as its limitations In this volume, the aim of the contributors is to share their analyses as a basis for more research in renewable energy technologies Basic to all the...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 2 pps
Ngày tải lên :
06/08/2014, 04:20
... be harvested and utilized as a fuel source This would be a disaster for agricultural ecosystems Without the protection of crop residues, soil loss may increase as much as 100-fold (Fryrear and ... each year and quantified as mass of carbon used to build stems, leaves and roots Note that in the large portions of South and East Asia, Western Europe, Middle East, and eastern US, humans grab ... million kcal of biogas is estimated to be $321, assuming the cost of labor to be $7/h; hence, the biogas has a value of $356 Manure processed for biogas has fewer odors and retains its fertilizer value...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 3 pot
Ngày tải lên :
06/08/2014, 04:20
... and some of the pasture classes The Remote Vegetation class comprises some of the USDA’s rangeland and pastureland classes The USDA forest class is somewhat larger than here, as some of the smaller ... determined by measuring the heat released between identical initial and final temperature of 25◦ C Petroleum, conventional: Petroleum, excluding lease gases and condensate, as well as tar sands, ... crops, grass, and trees, and translating the weighed or otherwise measured biomass into net primary productivity of ecosystems Both approaches yield very similar results 2.5.4 Satellite Sensor-Based...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 4 pot
Ngày tải lên :
06/08/2014, 04:20
... reported by those professionals was a tenth or less than the amount assumed in the multiplier-driven modeling systems that are commonly used (Swenson and Eathington 2006) Based on that research and ... demand for corn had increased by more than 150 percent As a consequence of the increased demand for ethanol, the projection for 2010 has the amount of corn available for feed as eight percent lower ... extensive systems for storage, marketing, and distributing that bounty The nation’s infrastructure for moving corn includes the inbound systems, the storage systems, grain processing systems, ...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 5 potx
Ngày tải lên :
06/08/2014, 04:20
... Treasury $15–30 million per year At least 15 states also provide assistance to establish new E85 facilities at retail gasoline outlets, as well as to support other ethanol distribution infrastructure ... subsidies not decline as the price of gasoline rises, as is the case for some subsidies benefiting petroleum and natural gas, and for some ethanol-support programs elsewhere, such as Canada (Steenblik, ... (and biodiesel) is classified as waste reduction and resource recovery plant (Class 49.5) under the Modified Accelerated Cost Recovery System (MACRS).16 This grouping includes “assets used in the...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 6 potx
Ngày tải lên :
06/08/2014, 04:20
... consequence of the decreasing energy returns as oil reservoirs are increasingly depleted and as there are increases in the energy costs as exploration and development are shifted increasingly deeper and ... and exploiting it As a consequence of the decreasing energy returns as oil depletion increases, and of the increasing energy costs as exploration and development shifted increasingly deeper offshore ... natural gas (considered together) Our preliminary results indicate that the EROI for global oil and gas (at least for that which was publically traded) was roughly 26:1 in 1992, increased to about...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 7 pdf
Ngày tải lên :
06/08/2014, 04:20
... of energy as the inputs that are needed for their construction from raw materials and subsequent maintenance — has already been assessed for wind turbines, and it has been put as low as six months, ... Denmark decreased, between 1990 and 2003, by 0.07% compared to an 8.4% decrease in the United Kingdom, which has only a 0.5% wind penetration Admittedly the decrease in the UK was almost entirely ... natural gas power station, but then one of the reasons that the company is reticent about costs is because it hopes to greatly reduce those costs as a result of increase in scale 18 It was hard...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 8 pdf
Ngày tải lên :
06/08/2014, 04:20
... contributors to municipal waste throughout the developed world are organic materials such as food waste, grass clippings, waste cooking oils, and paper (UNEP 2004b) Waste biomass that is presently ... presently destined for landfills has great appeal as a feedstock for biofuels production, as it is an available biomass source that does not compete with food Of this waste biomass, the BTL process can ... peculiarity of metabolic systems has to with an epistemic revolution associated with the development of non-equilibrium thermodynamics: living systems and more in general socio-economic systems are self-organizing...
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Biofuels, Solar and Wind as Renewable Energy Systems_Benefits and Risks Episode 1 Part 9 pps
Ngày tải lên :
06/08/2014, 04:20
... percentage of urban population in pre-industrial societies was very low As a matter of fact, when using biomass as primary energy source one has to rely on a power density of the energy input per square ... liters The assessment of labor demand for the phase of agricultural production is from Pimentel (2006), whereas the labor requirement for fermentation/distillation is based on two different assessments: ... not substantial discrepancies in the assessment of technical coefficients (inputs and outputs); both in phase I (of production of agricultural biomass) and in phase II (fermentation and distillation...
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