of dna damage and modulation of dna repair after exposure to gamma radiation
Báo cáo khoa học: "Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats" ppt
Ngày tải lên :
07/08/2014, 20:23
... [16] To investigate the changes of biomarkers after exposure to PAHs, we administered BaP, PH and PY, representative chemicals of the PAHs, to female rats We determined the concentration of BaP, ... suggested to reflect the total PAH contamination and is an indicator of the mutagenic activity of the PAHs in animals [5,6,22] In the human, the ratio of 1-OH-PY to 3-OH-BaP was reported to be 200 ... Short term exposure of rodent to disel exhausts; usefulness for studies of genotoxic and immunotoxic effect Chem Biol Interact 1999, 118, 19-38 30 Nisbet ICT, LaGoy PK Toxic equivalency factors (TEFs)...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
Ngày tải lên :
07/03/2014, 17:20
... because of the high content of Gln, Leu and Glu, we were able to accomplish complete side-chain assignment with the aid of additional HCCH-COSY spectrum After the automatic NOE assignment and structure ... regions of hHR23A and hHR23B is very high (88%), and the nine amino acids that differ [namely, N281(A) to D237 (B), Q287 (A) to N243 (B), I291 (A) to V247 (B), S297 (A) to A253 (B), I306 (A) to L262 ... characteristics of the 275–284 segment of hHR23B Because of the high quality of our 15 N-HSQC spectra, 54 of the 58 protonated backbone nitrogen atoms were available for relaxation measurements The values of...
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Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H. pylori DnaB helicase activity pptx
Ngày tải lên :
30/03/2014, 02:20
... HpDnaB by HpSSB is likely to be due to the inability of DnaB to bind the SSB-bound DNA EcSSB also shows an inhibitory effect on DNA dependent ATPase activity of EcDnaB when ssDNA is taken as substrate ... position of the ssDNA in the absence of SSB protein and the subsequent retardation of the band with an increasing amount of SSB is also shown *Position of the double-stranded control DNA (D) Localization ... towards ssDNA over dsDNA M13mp18 ssDNA or pUC18 dsDNA alone or a mixture of both was incubated either in the absence or presence of a different amount of HpSSB followed by separation of the DNA protein...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
Ngày tải lên :
09/08/2014, 09:20
... method to demonstrate that apoptosis induced by agarose is a component of total DNA breakage, and that the inhibition of apoptosis allows for more proper estimation of radiation- induced DNA DSB repair ... caspase inhibitors), and 3) reducing the interference of spontaneous breakage improves our ability to estimate DNA repair and to determine apoptosis intensity Methods Cell lines and tumour xenografts ... use of caspase inhibitors in pulsedfield gel electrophoresis may improve the estimation of radiationinduced DNA repair and apoptosis Radiation Oncology 2011 6:6 Submit your next manuscript to...
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Báo cáo y học: "Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus" pot
Ngày tải lên :
12/08/2014, 15:23
... leucocytes less efficiently repair radiation- induced DNA damage and that DNA repair gene polymorphic sites may predispose to the development of particular clinical and laboratory features such as neuropsychiatric ... C, Sakamoto-Hojo E, Donadi E: Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, ... 6,150/mm3 (range 2,900 to 12,230/mm3 ) The median for the total number of and the proportion of neutrophils was 4,928/mm and 80.0%, respectively (range 1,601 to 9,674/mm3 and 55.2 to 90.1%), while...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
Ngày tải lên :
24/08/2014, 09:33
... target of DNA- PK55 Due to its ability to bind ssDNA, RPA has been shown to be involved in several nuclear pathways including DNA replication and DNA repair2 In terms of the repair of double strand ... these genomic stresses, DNA repair pathways have evolved to deal with a variety of DNA lesions Of these, DNA double strand breaks are a particularly toxic form of DNA damage in that a single ... harmful effects of DNA damage, DNA repair mechanisms have evolved to eliminate or at the very least, minimize DNA damage Specific pathways have evolved to deal with distinct forms of DNA damage Base...
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FUNCTIONAL ANALYSIS OF TWO NOVEL DNA REPAIR FACTORS, METNASE AND PSO4
Ngày tải lên :
24/08/2014, 10:50
... major pathway for DNA repair in mammals due to its cell cycle independence Briefly, Ku70/80 binds the broken ends and DNA- PKcs moves to the site of damage and is autophosphorylated After end processing, ... to bring Metnase to the DSB sites for its function(s) in DNA repair Aim In vitro analysis of Metnase and/ or hPso4 binding to dsDNA: I showed that Metnase exists as a dimer, and forms a 1:1 stoichiometric ... and DNA- PKcs, and is sensitive to wortmannin Most importantly, it provides a unique tool to measure joining of various compatible and non-compatible ends, which is crucial for understanding of...
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ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU80 C-TERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU
Ngày tải lên :
24/08/2014, 11:02
... THE ROLE OF REDOX EFFECTS AND THE KU80 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks (DSB) are among the most lethal forms of DNA damage and can ... (NHEJ) DNA Repair Pathway A DSB occurs and the pathway is initiated by the Ku protein binding damaged DNA termini, translocates inward, and recruits DNA- PKcs to form the DNA- PK heterotrimer DNA- PK ... activation of DNA- PKcs, and only the final 12 amino acids are sufficient to bind DNA- PK (31-33) Upon removal of the C-terminus of Ku80 the kinase activity of DNA- PK is drastically decreased Due to the...
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Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
Ngày tải lên :
30/03/2014, 15:20
... molecule of PM2, I ¼ the percentage of supercoiled PM2 DNA, and II ¼ the percentage of open circular PM2 DNA The overall number of strand breaks per 10 000 bp represents the sum of single-strand breaks ... discarded and the DNA pellet resuspended in enzyme buffer Damage induction and all subsequent steps were carried out in the dark to prevent additional DNA damage Oxidatively damaged PM2 DNA (300 ... include factors involved in DNA damage signaling and repair, such as poly(ADP-ribose) polymerase-1, formamidopyrimidine -DNA glycosylase (Fpg), which is involved in the repair of certain types of oxidative...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
Ngày tải lên :
31/03/2014, 21:21
... involved in the Topo I DNA repair pathway, and inhibitors of TDP may have therapeutic utility in treating cancers that are refractory to camptothecin treatment In order to understand the structure–activity ... known inhibitors to PLD1 and PLD2 (data not shown) When Topo I is inhibited by camptothecin and cancer chemotherapeutic agents, covalent Topo I DNA complexes accumulate and cause cytotoxic effects ... nucleotide changes of A378 to T and G481 to A lead to amino acid substitutions of R126S and G161R, respectively Two human TDP variants containing deletion of N-terminal 39 (huTDPND39) and 174 (huTDPND174)...
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Báo cáo hóa học: " Synthesis and characteristics of NH2-functionalized polymer films to align and immobilize DNA molecules" potx
Ngày tải lên :
20/06/2014, 23:20
... technique is very difficult to control The technique of controlling and manipulating DNA molecules with nanometer resolution is a subject of priority in the field of DNA- based nanotechnology; many ... Inc., Sunto-gun, Shizuoka, Japan) DNA alignment was carried out according to the tilting method, and this resulted in highly aligned DNA patterns on the substrate [16, 18] A λ -DNA solution of 10 ... polymer film, and the sample was then tilted to almost 90° DNA molecules were stretched and aligned along the direction of the sample's tilting For the synthesis of the conductive nanowire, DNA molecules...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
Ngày tải lên :
28/06/2014, 05:20
... Pathways to Fixing DNA Damage and Errors the tumor genetic background with regard to the DNA repair machinery status (Antoni et al., 2007), and ii) the status of the various constituents of the DNA repair ... fragment processing: modulation of the strand displacement activity of DNA polymerase delta by the concerted action of 20 DNA Repair − On the Pathways to Fixing DNA Damage and Errors replication ... the recruitment of DNA repair enzymes and triggers the activation of cell cycle checkpoints, cell cycle arrest or apoptosis 30 DNA Repair − On the Pathways to Fixing DNA Damage and Errors 3.3...
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Báo cáo y học: " Differential patterns of intronic and exonic DNA regions with respect to RNA polymerase II occupancy, nucleosome density and H3K36me3 marking in fission yeast" pps
Ngày tải lên :
09/08/2014, 23:20
... Promoter and terminator regions are taken as 400 bp up- and downstream of the start and stop codons, respectively, and divided into 10 bins of 40 bp each, while the coding regions were divided into ... coding, and terminator regions; in the case of spliced genes, the ORF was further divided into exons and introns For both unspliced and spliced genes, 400-bp regions upstream of the start of the ... start of the ORF and downstream of the end of the ORF were taken as the promoter and terminator, respectively These 400-bp windows were divided into ten bins of 40 bp each, and Affymetrix probes...
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Báo cáo y học: "A general framework for quantifying the effects of DNA repair inhibitors on radiation sensitivity as a function of dose" pptx
Ngày tải lên :
13/08/2014, 16:21
... Secondly, cytotoxic effects of sensitising agents that are independent of radiation are not taken into account Such effects may be small, and are often concealed by the method used to calculate ... denominator This method revealed radiation- independent drug effects and enabled assessment of the relationship of this variable to radiationdependent effects Conclusion Measurement of radiation ... inhibitor of poly(ADP-ribose) polymerase and radiation sensitizer Int J Radiat Biol 1999, 75:91-100 Boulton S, Kyle S, Durkacz BW: Interactive effects of inhibitors of poly(ADP-ribose) polymerase and...
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INHIBITION OF APE1’S DNA REPAIR ACTIVITY AS A TARGET IN CANCER: IDENTIFICATION OF NOVEL SMALL MOLECULES THAT HAVE TRANSLATIONAL POTENTIAL FOR MOLECULARLY TARGETED CANCER THERAPY
Ngày tải lên :
24/08/2014, 13:10
... deficiency of a particular DNA repair pathway in cancer cells can lead to elevated levels of other DNA repair pathway proteins leading to efficient repair of DNA damage and reducing the efficacy of cancer ... chemotherapeutic agents and radiation are related to their ability to induce DNA damage, and the ability of cancer cells to identify and efficiently repair such DNA damage undermines the efficacy of these agents ... efficiency of the current chemotherapy and radiation regimens CHAPTER II REVIEW OF RELATED LITERATURE Importance of DNA Repair Pathways and Cancer DNA repair pathways protect the genome from damage...
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Role of BRCA2 in DNA repair
Ngày tải lên :
16/10/2015, 15:35
... Figure 4, BRCA2 recruits Rad51 to sites of DNA damage and promotes nucleation of the Rad51 filament to sites of DNA double strand breaks 21 Figure 5, BAC DNA integrity is intact after successful 105 bp deletion ... addition, BRCA2 also appears to control the enzymatic activity of RAD51. Figure 4, BRCA2 recruits Rad51 to sites of DNA damage and promotes nucleation of the Rad51 filament to sites of DNA double strand ... catalytic subunit (DNA PKcs). The DNA Ligase IV complex, consisting of the catalytic subunit DNA Ligase IV and its cofactor XRCC4, performs the ligation step of repair. DNA PKcs is thought to mediate end bridging. The Pol X family DNA polymerases Pol ...
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Tài liệu Báo cáo khoa học: "The impact of language models and loss functions on repair disfluency detection" pptx
Ngày tải lên :
20/02/2014, 04:20
... and defines three distinct parts of a speech disfluency, referred to as the reparandum, the interregnum and the repair Consider the following utterance: reparandum Related work I want a flight to ... related to those in the repair Indeed, in our training data we find that 62% of the words in the reparandum are exact copies of words in the repair; this identity is strong evidence of a repair ... always be present; and up of disfluent material Although they are inter- the repair to Denver replaces the reparandum ested not only in disfluency but also a syntactic analShriberg and Stolcke (1998)...
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Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
Ngày tải lên :
20/02/2014, 11:20
... protein-containing bands indicated covalent attachment of DNA to the enzyme The yield of the photochemical cross-linking reaction was calculated as the ratio of the covalent conjugate radioactivity to the total ... bearing a photolabile TFMDPh group, was introduced into the oxoG-containing strand of a 29/22-mer DNA duplex 5¢ to oxoG (duplex I) or 5¢ to the oxoG-pairing cytidine residue of the other strand (duplex ... residue instead of oxoG This duplex was used to check whether the binding of DNA duplexes I and II to the Fpg protein was specific A 29-mer oligonucleotide used to prepare DNA duplex I, and containing...
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Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt
Ngày tải lên :
05/03/2014, 23:20
... PolC and DnaE The ability to bind single-stranded DNA has indeed been demonstrated for the E coli DnaE OB domain [12,16] The very N-terminal region of PolC and the C-terminal domain of DnaE appear ... domain and the additional N- and C-terminal regions in PolC and DnaE, respectively The PolC proofreading 3¢–5¢ exonuclease domain is inserted into the PHP domain and is an integral part of the ... shown to be a major double-stranded DNA binding determinant in the E coli DnaE [12] Crystal structures revealed that this motif binds double-stranded DNA similarly in both PolC [8] and DnaE [6]...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
Ngày tải lên :
07/03/2014, 15:20
... involved in DNA repair and cell cycle checkpoint regulation on DNA damage [13] shows a lower degree of homology to human Pol k To determine the phylogenetic relationship between OsPol k and other ... of DNA polymerase inhibitors on the DNA polymerase activity of OsPol k Strong inhibition was noted with 2,3-dideoxythymidine-5-triphosphate, a known inhibitor of mammalian DNA polymerases b and ... BER but also in DNA doublestrand break repair and meiosis, and Pol b and TdT may be evolved for differentiation and development of specific organs in vertebrates such as the nervous and immune systems...
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