observed inhibition of neutrophil activation by surfactant with sp b c

Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elaﬁn and pre-elaﬁn (trappin-2) expressed in Pichia pastoris ppt

... AGA GCG CAA GAG CCA GTC AA-3Â, restriction site XhoI This primer fuses the elan mature protein immediately downstream of the a-peptide sequence Primer 3; 5Â-CGAGCGGCCGCCCCTC TCA CTG GGG AAC-3Â, ... its complex with rec-elan Complexes were rst formed by incubating equimolar concentrations (10)6 M) of enzyme and inhibitor Dissociation of the complexes was induced by dilution in a concentrated ... nonreducing conditions (b) or reducing conditions ( +b) Molecular masses of the protein standards are shown on the left Fig Inhibition of the enzyme activity of pancreatic elastase by recelan Constant...

Báo cáo khoa học: Endogenous tetrahydroisoquinolines associated with Parkinson’s disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines doc

Danh mục: Báo cáo khoa học

... Formation of a TH–Fe(III)–catecholamine complex induces an absorbance change at 700 nm that can be detected using visible spectroscopy Under anaerobic conditions, the absorbance change is only 50% of ... 2A) A kinetic analysis indicated that NMNorsal reduced hTH activity by competing with the essential pterin cofactor (Fig 2B) ; hTH inhibition by NMNorsal was noncompetitive with respect to tyrosine ... inhibition by NMNorsal or Norsal (n = 4) tetrahydropterin cofactor and an almost irreversible blockade of TH activity by facilitating oxidation of the catalytic iron [21,23] As catecholamine-bound...

Báo cáo Y học: Concentration-dependent reversible activation-inhibition of human butyrylcholinesterase by tetraethylammonium ion pptx

Danh mục: Báo cáo khoa học

... (Eur J Biochem 269) Ó FEBS 2002 Fig Dependence of the activity of various human BuChEs and horse BuChE on the concentration of TEA at various butyrylthiocholine concentrations BTC concentrations ... performed with a constant dielectric constant (e ¼ 1) Electrostatic force was treated without cutoffs and van der Waals forces were ˚ calculated with the shift method with a cutoff of 10 A All ... Pathways of ligand clearance in acetylcholinesterase by multiple copy sampling J Mol Biol 298, 705–726 Cleland, W.W (1977) Determining the chemical mechanisms of enzyme-catalyzed reactions by kinetic...

Báo cáo y học: "Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon a" pptx

Danh mục: Báo cáo khoa học

... transcription by HBc protein would decrease the inhibitory effect of IFN-a on HBV replication pCMV-HBc (WT), pCMV-HBc (L60V), pCMVHBc (S87G), pCMV-HBc (I97L) and pCMV-Tag1 were transfected into Huh7 cells ... hot-spot mutants has no impact on inhibition of HBV replication by IFN-a, indicating MxA may be independent of inhibition of HBV DNA replication by IFN-a The clinical significance of the inhibitory ... by IFN-a In order to evaluate whether the inhibition of MxA transcription by HBc protein could decrease the 2.1 IFN A inhibitory effect on HBV replication induced by IFN-a, pU19-1.24HBV was co-transfected...

Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt

Danh mục: Báo cáo khoa học

... subunits of active caspase-8 [25,26] Caspase-8 in turn is responsible for initiating a cascade of activation of effector caspases that ultimately leads to the multiple changes in cells characteristic ... characteristic of TRAIL-induced apoptosis [27] The process of activation of caspase-8, and the downstream consequences that arise from it, are blocked by the caspase-8-speci c peptide inhibitor zIle-GluThr-Asp-ﬂuoromethyl ... functionally active isoforms, caspase-8 ⁄ a and caspase-8 ⁄ b J Biol Chem 272, 26953–26958 25 Sohn D, Schulze-Osthoff K & Janicke RU (2005) Caspase-8 can be activated by interchain proteolysis without...

Báo cáo khoa học: Dual modulation of prothrombin activation by the cyclopentapeptide plactin pptx

Danh mục: Báo cáo khoa học

... signiﬁcant increase in scu-PA activation on U937 cells (Fig 3B, C) Plactin D affected a-thrombin-mediated scu-PA activation only slightly (Fig 3B) Hirudin, a speci c inhibitor of Dual modulation of ... including U937 cells Therefore, we examined the effects of cystatin, an inhibitor of DPP-I, on tcu-PA ⁄ T activation by U937 cells Cystatin effectively inhibited tcu-PA activation by U937 cells (Fig ... susceptible to activation by Xa Under physiological coagulation conditions, prothrombin activation is catalyzed by the prothrombinase complex (factor Xa in complex with factor Va, phospholipids and Ca2+)...

Báo cáo khoa học: A new and efﬁcient method for inhibition of RNA viruses by DNA interference pptx

Danh mục: Báo cáo khoa học

... infected tobacco leaves by RT-PCR, using primers TMV1 (5¢-GCCCAAGGTGAACT TTTCAA-3¢) and TMV2 (5¢-TAGCGCAATGGCATACA CTC-3¢) The sequence of as-DNA is 5¢-CAATACTGTCT TTCTGGCCTTC-3¢, and that of ... 5¢-ATAGGCG GGAATTTTGCATC-3¢ Anti-TMV siRNA was synthesized using the DNA templates 5¢-AAGGGACGAGCA TATGTACACCCTGTCTC-3¢ and 5¢-AAGTGTACATAT GCTCGTCCCCCTGTCTC-3¢ and a Silencer siRNA Construction ... 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; antisense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTG TCTC-3¢) and a Silencer siRNA Construction Kit (Ambion) Acknowledgements This work was supported by grants from...

Báo cáo Y học: Growth inhibition of mammalian cells by eosinophil cationic protein pptx

Danh mục: Báo cáo khoa học

... observe the speci c binding using ECP and RITC-labeled ECP by monitoring the level of ¯uorescence, but the change in ¯uorescence level caused by the competition of ECP and RITC-labeled ECP could ... of cell surface receptors for ECP We could not show the presence of a speci c high-af®nity binding site for ECP on cells by competition assay This is probably because the af®nity of ECP for binding ... The number of colonies was counted, and the area occupied by the colonies was evaluated by image scanning assisted by a computer Fluorescence microscopy ECP and bovine RNase A were labeled with...

Báo cáo y học: " Regulation of Sox9 activity by crosstalk with nuclear factor-κB and retinoic acid receptors" pptx

Danh mục: Báo cáo khoa học

... Therapy Vol 10 No Rockel et al containing the rat Col2a1 48-bp minimal enhancer (5'CTGTGAATCGGGCTCTGTATGCACTCGAGAAAAGCCCCATTCATGAGA-3'), described by Lefebvre and coworkers [29], was obtained from Invitrogen ... (compare Figure 7b and Figure 4b; P < 0.001) To determine the effect of NF- B activation by caMEKK1 on RAR function, cells were co-transfected with caMEKK1 and I B- 2N I B- 2N inhibits NF- B activation ... thereby suppressing NF- B binding to DNA This mechanism could contribute to the reduction in binding to DNA of the TNF-α-activated complex observed under conditions in which chondrocytes were subjected...

Báo cáo y học: " Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos" pptx

Danh mục: Báo cáo khoa học

... Amplification primers for each specific mRNA were deduced from published sequences and included bcl2 (P1: 5’-GTTCCACCCGTTTTCA-3’, P2: 5’-GCGAGTCCTCATTCTGT-3’), bad (P1: 5’-CA AGCCTGGATAAACAC-3’, ... min, sections were incubated with target-purified rabbit primary antibodies, including BCL2 (Anaspec Inc., Fremont, CA, USA), BAD (Anaspec Inc., Fremont, CA, USA), CASPASE-3 (Anaspec Inc., Fremont, ... sac B: In tbx5 knockdown embryos, a string-like cardiac morphology was observed, with no looping formation Most of the cardiac sac was occupied by pericardial effusion C: No apoptosis was observed...

báo cáo khoa học: " Inhibition of telomerase activity by HDV ribozyme in cancers" ppsx

Danh mục: Báo cáo khoa học

... double-sranded DNA of g RZ57 was synthesized with ApaΙ and HindIII protruding ends Their sequences are as follows: 5’ AGCTT GGGAC CACCA CCACG CGGAC GCAAG AAGGG CAAGC GGCAA CGCAA GGCAA AGGGACCC CCC ... CAAGC GGCAA CGCAA GGCAA AGGGACCC CCC 3’ and 5’ A CCCTG GTGGT GGTGC GCCTG GCTGG TCCCG TTCGC CGTTG CGTTC CGTTT CCCTG GG GGG 3’ The predicted secondary structure of g RZ57 are seen in Figure After ... generated by RT-PCR of a 184 bp fragment by of hTR cDNA by digestion of Recombinant plasmid pBBS212 containing hTR gene (provided Ribozyme transfected bel7402 cells and HCT116 cells showed decrease of...

Báo cáo y học: "Inhibition of neutrophil activity improves cardiac function after cardiopulmonary bypass" pot

Danh mục: Báo cáo khoa học

... specific detection of neutrophils Effects of LIM on cardiac function The cardiac function has been analyzed by the thermodilution and conduction catheter technique As shown in Figure 1, the cardiac ... http://www.journal-inflammation.com/content/4/1/21 σ specific conductance of blood G(t) left ventricular conductance G(p) parallel conductance A pressure tip transducer in the conductance catheter measures left ventricular pressure ... global distribution of neutrophils within the body after passing the LIM, technetium-labeled neutrophils were injected into the blood circulation before onset of CPB or CPB with LIM (n = 2, each...

Báo cáo khoa học: "Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris" pdf

Danh mục: Báo cáo khoa học

... in vitro, inhibition of the specific targets responsible for anti-HIV activity of Prunella in cells remains unclear Identification of constituents of P vulgaris that confer the inhibition to ... Cytotoxicity of fraction was in contrast to what we had observed with similar concentrations of aqueous extract suggesting that either cytotoxic compounds were being concentrated in this fraction ... size exclusion fractions (100 μg/mL) (A), ethanol-soluble sub-fractions (B) , ethanol-insoluble sub-fractions (C) , or a combination of both the ethanol soluble and insoluble fractions at concentrations...

Báo cáo y học: " Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome" pps

Danh mục: Báo cáo khoa học

... 104) B 35 30 25 20 15 10 HIV HIV-lhNef Luc-Nef + Figure Test of sequence-specificity of the inhibition by HIV-lhNef Test of sequence-specificity of the inhibition by HIV-lhNef (A) Trans -inhibition ... mononuclear cells by specific RNA interference Nucleic Acids Res 2002, 30:4830-4835 Anderson J, Banerjea A, Akkina R: Bispecific short hairpin siRNA constructs targeted to CD4, CXCR4, and CCR5 confer ... induce a potent HIV-1-specific RNA silencing response in cells Tests with reporter constructs not support a sequence-specific RNAi effect, although the effect induced by lhNef is HIV-1 specific...

Báo cáo y học: " Inhibition of Tat activity by the HEXIM1 protein" docx

Danh mục: Báo cáo khoa học

... than 10% inhibition The specific effect of HEXIM1 expression was highlighted by the results shown in Fig 2B G5HIV-Luc reporter was activated by co-expression of a GAL4-TBP, and such activation ... absence of Tat [20,21] We sought to determine the consequences of ectopically expressed F:HEXIM1 on P-TEFb induced transcription in the absence of Tat We showed that direct recruitment of CyclinT1 ... for efficient Tat inhibition Results Tat activity is inhibited by HEXIM1 Tat activity involves direct interaction with CDK9/ CyclinT1 (P-TEFb) complex However, two major P-TEFbcontaining complexes...

Inhibition of copper corrosion by orthophosphate a mechanistic study

Danh mục: Tổng hợp

... an electrochemical process, electrochemical techniques can be used for investigating its mechanistic details The suggested electrochemical mechanism of copper corrosion inhibition could be verified ... will become: Cu+ Cl- → CuCl0+ e- (2.4) CuCl0 + Cl- → CuCl2- (2.5) The dissolution process is controlled by the mass transfer of CuCl2- from the electrode surface to the bulk solution The reactions ... 2.2 Phosphate as Corrosion Inhibitor 2.2.1 The Application of Phosphates Phosphates can be applied as phosphoric acid, combinations of orthophosphoric acid, zinc orthophosphate and polyphosphate...

Báo cáo y học: "Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System"

Danh mục: Y học thưởng thức

... virulence [15] On the other hand, the decay-accelerating factor (DAF) with its well-established role in the inhibition of activation of complement cascade [16] has recently been shown to be a co-receptor ... screening of cellular proteins interacting with HBsAg Figure Determination of Size of Inserts in cDNA Library Constructed from HBV-transfected HepG2 Cells The construction of this cDNA library from HBV-infected ... the ubiquitin complex This complex will then be recognized by UBPs and the TF that is located at the C terminus of Cub will be cleaved off and result in downstream reactions, which is the activation...

EFFECT OF INHIBITION OF S-NITROSOGLUTATHIONE REDUCTASE ON THE NF-κB PATHWAY

Danh mục: Khoa học tự nhiên

... Inhibitors of GSNOR 14 Inhibition of A549 cells by C2 using BrdU Incorporation 27 Inhibition of A549 cells by C3 using BrdU Incorporation 28 Inhibition of A549 cells by C3 with ... 7.5 Dodecanoic acid 19 212 C1 78 93 1.3 C2 55 91 2.4 C3 75 95 1.1 Table In vitro Percent Inhibition and IC50 of C1 , C2 , C3 The percent inhibition was determined using conditions Inhibition studies ... REFERENCES 53 CURRICULUM VITAE vii LIST OF TABLES In vitro Percent Inhibition and IC50 of C1 , C2 , and C3 11 Compound Inhibition Comparison of ADHs 12 Treatments of RAW 264.7 cells...

Báo cáo khoa học: Suppression of urokinase receptor expression by bikunin is associated with inhibition of upstream targets of extracellular signal-regulated kinase-dependent cascade pptx

Danh mục: Báo cáo khoa học

... sequence of 500 bases from this vector, an inverted PCR was performed with primers (P3: 5¢-GAGAG TCAGCGCTGCTGTGCTACCCCAAGA-3¢, P4: 5¢-ACT TGGATGTTGTCGGGCGGCGTTGGCACA-3¢) The resulting PCR product ... (P1: 5¢-ACCG AGCCTCGAGGATATACCAAGGCAGAGGAGC-3¢, P2: 5¢-ACTTGCAGAGCGGCCGCTTGTCAGTTGGA GAAGC-3¢) Cloned cDNA was inserted into the XhoI– NotI site of the pCIneo vector (Clontech) In order to remove ... transfection into the HRA cell line The a1 microglobulin-bikunin cDNA was cloned by PCR using the human liver cDNA library (Clontech, Palo Alto, CA, USA) as a template with primers (P1: 5¢-ACCG AGCCTCGAGGATATACCAAGGCAGAGGAGC-3¢,...

Báo cáo khoa học: Inhibition of a-synuclein ﬁbrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot

Danh mục: Báo cáo khoa học

... HSQC spectra of the reactions of a-Syn with quinone and DA (A) Overlay of the 1H-15N HSQC spectra of a-Syn (black) and the reaction product of a-Syn and Q (red) (B) Overlay of the 1H-15N HSQC spectra ... courses of the fibrillization processes of a-Syn incubated with the DA-analog compounds were monitored by a thioflavin (ThT) fluorescence assay [17] Figure displays inhibition of a-Syn fibrillization by ... (left) Table Molecular masses of the monomeric adducts as determined by electrospray mass spectrometry Molecular masses were of the peaks detected by ESI-MS Pattern shows incremental molecular masses...

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