nuclear reprogramming of somatic nucleus hybridized with embryonic stem cells by electrofusion pdf
Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... By contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... replication timing profile of mouse ES cells We show that, while early replication in ES cells correlates with peaks of increased histone acetylation, the replication times of many, but not all, ... ES cells for 24-48 h with doses of the HDAC inhibitor Trichostatin A (TSA), which raised the global levels of histone acetylation in nuclei (as judged by immunofluorescence; data not shown) without ... consequence of satellite transcription refereed research Figure replication in ES cells is altered by mutation of chromatin modifiers Satellite Satellite replication in ES cells is altered by mutation of...
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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc
... Super-infection of ES cells by co-cultivation (a) Efficiency of infection by co-cultivation with the viral producer cell line B4-5 AB2.2, wild-type (WT), and NGG5.3 Blm-deficient embryonic stem cells cells ... an approach to generate cells with homozygous mutations, which increases the complexity of most genetic screens because of the low rate of loss of heterozygosity (LOH) of single allelic mutations ... the frequency of noninfected cells (b) Assessment of proviral copy number in randomly picked nonselected wild-type and Blm-deficient embryonic stem (ES) cells after co-cultivation with the viral...
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The role of kruppel like factors in embryonic stem cells
... embryonic carcinoma cells 1.1.2 Mouse embryonic stem cells 1.1.3 Mouse embryonic germ cells and pluripotent spermatogonial stem cells 1.1.4 Mouse EpiSCs and FAB stem cells 1.1.5 Pluripotent stem ... application of stem cells which has been impeded by lack of efficient and straightforward ways to obtain the patient-specific stem cells Introduction 1.1 Pluripotent stem cells Pluripotent stem cells ... all of specialized cell types Only one type of cells can be derived from unipotent stem cells Mouse embryonic stem (ES) cells are pluripotent cells with various applications For example, they...
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Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf
... and nuclear transfer of mPER3 [33] Phosphorylation of mPER1 itself correlates with nuclear transport [34] Earlier studies had already indicated that nuclear translocation of mPER3 is promoted by ... HEK293 cells; CKIe-mediated phosphorylation of mPER1 was reported to lead to masking of the nuclear localization signal (NLS) and coexpression of mPER1 with mPER2 and cytoplasmic localization of ... imported into the nucleus of injected oocytes (Fig 1B) S Loop and T Pieler The absence of nuclear import of mPER3 injected into Xenopus oocytes suggests that the protein is devoid of a nuclear import...
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Báo cáo y học: "High nuclear expression of STAT3 is associated with unfavorable prognosis in diffuse large B-cell lymphoma" doc
... STAT3 nuclear expression Figure STAT3 expression and P-STAT3 level in DLBCL (A) negative nuclear staining of STAT3, (B) negative nuclear staining of P-STAT3, (C) weak nuclear staining of STAT3, ... had the STAT3 expression, including 19 cases (25.7%) with strong nuclear staining of STAT3, and 24 cases (32.4%) with strong nuclear staining of P-STAT3 Representative staining outcomes were shown ... accuracy of our IHC analysis (Table 2) Correlation between the nuclear expression of STAT3 and clinicopathological parameters We observed the associations of the STAT3 nuclear expression with IPI...
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THE DIRECT REPROGRAMMING OF SOMATIC CELLS: ESTABLISHMENT OF A NOVEL SYSTEM FOR PHOTORECEPTOR DERIVATION
... as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) or neural stem cells [14–17, 19–21] These protocols are often based upon culturing the stem cells in culture medium with ... neuralization of somatic cells induced by combinations of known pro-neural genes, and finally, the induction of photoreceptor-like phenotypes in somatic cells 2.1 Selection of candidate genes While many of ... differentiated, somatic cells to a mitotically active pluripotent state Thus, he tested the effects of viral delivery of combinations of transcription factors known to be active in embryonic stem cells...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor FISH ... expression of SSEA-3 was indicated by green fluorescence under a wavelength of 490 nm (FITC), while positive expression of TRA-1-81 was indicated by red fluorescence under a wavelength of 570 nm ... Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: somatic...
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Tài liệu Báo cáo "REMARKS ON LOCAL DIMENSION OF FRACTAL MEASURE ASSOCIATED WITH THE (0, 1, 9) - PROBLEM " pdf
... If y6k+4 = 1, by similar argument as the proof of the cases i = 2, we have # t(6k + 8) F6k+8 The proposition is proved Proof of the main theorem 4.1 Claim For s ∈ supp µ is defined by s = S(x0 ... representation of sn through points in supp µn−1 and sn = sn−1 + 30 or sn = sn−1 + 39 n n or both of them, where sn−1 , sn−1 , sn−1 in supp µn−1 Proof It is proved similarly as proof of Claim 2.1, ... i is odd}, Remarks on local dimension of fractal measure associated with ej = ki ; oj = i∈Ej 49 ki i∈Oj Then nj = oj + ej Similar proof as the proof of Claim 3.2 in [11], we get j nj−1 oj...
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Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx
... of proBMP-2 to BMP-2 over time, possibly by proteases secreted from the C2C12 cells, rather than an AP-inducing activity of proBMP-2 Contamination of the proBMP-2 protein sample with traces of ... demonstrate binding of proBMP-2 to the ECD of BMPR-IA Interaction of BMP-2 (A) and proBMP-2 (B) with immobilized ECD of BMPR-IA Interaction of BMP-2 (C) and proBMP-2 (D) with immobilized noggin ... association of mature BMP-2 with the ECD of BMPR-II, probably by masking binding sites of BMP-2 Consistently, maximal inhibition was observed by using equimolar concentrations (0.4 lm) of both pro-peptide...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... interaction of RNA Pol II with the b-globin gene locus in mouse embryonic fibroblasts (MEFs) and OP9 stromal cells (OP9) These cells were used in our subsequent studies to support the growth of undifferentiated ... differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells to gen-...
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differentiation of embryonic stem cells
... from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells 13 ... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Embryonic Germ Cells 25 Isolation and Culture of Embryonic Germ Cells MARIA P DE MIGUEL AND PETER J DONOVAN 353 Section III Gene Discovery by Manipulation of Mouse Embryonic Stem Cells 26 Gene...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion with VEGF expansion with PDGF - BB VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated ... presence of VEGF and 10% FCS and expanded by about 85-fold after passages The expanded cells at 5th passage were constituted with two cell fractions One of these cells was VE-cadherin+ cells (35–50%),...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by ... expressed in hES cells (Figure 7) The expression of OCT4 by hES cells was upregulated by 12fold, LEFTY1 by 70-fold and TDGF1 by 19-fold compared to EB Compared to adult cells, expression of OCT4 in ... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational...
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Báo cáo hóa học: " The association of complex liver disorders with HBV genotypes prevalent in Pakistan" pdf
... tube containing 50 µl of a reaction buffer made up of the following components: 50 ng each of the outer primer, a 200 µM concentration of each of the four deoxynucleotides, 1U of Taq DNA polymerase ... world are infected by HBV [2], More than 350 million people are chronic carriers of the virus [3] Acute hepatitis of varying severity exists in 95% of children and 2–10 % of adult patients [4] ... prognosis of the inactive carrier is generally good and well supported by long-term follow-up studies [31-33] An estimated 20% to 30% of HBsAg carriers may develop reactivation of hepatitis B with...
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báo cáo hóa học:" Augmentation of tibial plateau fractures with Trabecular Metal™: a biomechanical study" pdf
... for both construct with anatomic reduction of the End result for both construct with anatomic reduction of the osteochondral fragments In static loading, the failure properties of each constructs ... et al.: Biomechanical testing of buttress and raft plate fixation of lateral tibial plateau fractures Proceedings of the 64th Annual Meeting of the American Academy of Orthopaedic Surgeons: Atlanta, ... shaped as a disc (20 mm of diameter by mm of thickness) The TM disc was placed over the two subchondral 4.5 mm screws and under the articular fragments as a subchondral augment with no graft For both...
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báo cáo hóa học:" Gender associated differences in determinants of quality of life in patients with COPD: a case series study" pdf
... and then matched 73 patients with similar degree of airflow obstruction randomly selected from our much larger population of men with COPD Patients with all degree of airflow severity were included ... enrolled, 25% of the men and 23% of the women were still smoking None of the patients had a history of exposure to biomass fuel Using the GOLD staging system [22] we have equal number of men and ... significant level with a power of 85%, considering a median SGRQ value of 30 points for men and 40 for women Page of (page number not for citation purposes) Health and Quality of Life Outcomes...
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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx
... purpose of this paper is to introduce and to analyze the performance of different array receivers for the detection of a known signal, with different sets of unknown parameters, corrupted by an ... detection of a known rectilinear signal with known parameters corrupted by a SO noncircular total noise is presented as a reference receiver, jointly with some of its performance Various extensions of ... ^ signal (SINRo
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... of intracellular reactive oxygen species The cells were pretreated with or without DHT for 30 and Trypan blue exclusion assay The cells were pretreated with or without DHT for 30 and Effect of ... of lipid peroxides The cells were pretreated with or without DHT for 30 and then were treated with H2O2 for to 120 The level of LPO in the monolayer cells was determined by measuring the malondealdehyde ... of nucleoside analogues with the sodium-nucleoside transport system in brush border membrane vesicles from human kidney Pharm Res 1993, Effect of dihydrotestosterone on mouse embryonic stem cells...
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