nuclear reprogramming and induced pluripotent stem cells

báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

... for making induced pluripotent stem cells: reprogramming a la carte Nat Rev Genet 2011, 12:231-242 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... Teitell MA, Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini...

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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells

Danh mục: Y - Dược

... (Figure and 3): Oct4, Sox2, Klf4, and c-Myc (OSKM)48 These converted cells, which share morphological and pluripotent characteristics with ESCs, are known as induced pluripotent stem cells (iPSCs) ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs),...

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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps

Danh mục: Báo cáo khoa học

... and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F, Beard C, Gao Q, ... CT: Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki...

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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot

Danh mục: Báo cáo khoa học

... for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic ... clearly different from reprogramming through somatic cell nuclear transfer or fusion with pluripotent cells The efficiency and kinetics of reprogramming are not the same, with reprogramming by defined ... mouse induced pluripotent stem cells without viral vectors Science 2008, 322:949-953 Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K: Induced pluripotent stem cells generated without viral...

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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages

Danh mục: Cao đẳng - Đại học

... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent ... thigh region, and used lentiviruses to induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers...

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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

Danh mục: Báo cáo khoa học

... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... deletions and duplications [63-64], somatic coding mutations [65], and chromosomal duplications [66] hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent...

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Atlas of Human Pluripotent Stem Cells doc

Danh mục: Sức khỏe giới tính

... Human Pluripotent Stem Cells Derivation and Culturing, summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and ... human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early human development and organogenesis, ... karyotyping and ﬂuorescent in situ hybridization (FISH) methods as they apply to the ﬁeld of pluripotent stem cells Induced pluripotent stem cells attract great interest for their potential in understanding...

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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Danh mục: Y - Dược

... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... Joint Symposium on Stem Cells and Bioimaging, 2425 May 2010, Singapore Y Liu, SH Teoh, SK Chong, Z Zhang, MA Choolani, J Chan Human endothelial progenitor stem cells accelerates and potentiates ... 60 2.8.4.1 Hypoxia and Mesenchymal Stem Cells 60 2.8.4.2 Hypoxia and Bone Repair 61 2.9 Coculture Systems 62 2.9.1 Trends in Coculture Systems 62 2.9.2 Cocultures...

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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS 2

Danh mục: Cao đẳng - Đại học

... GFP tag was cultured and allowed to differentiate so that there will be a mixture of stem cells and differentiated cells CDy9 was added to this semi-differentiated cells and it was observed that ... 10: CDy9 and immunocytochemistry staining of a mixture of differentiated cells and GFP-miPSCs (a) and (b) are 10X and 40 X images respectively CDy9 signal is only noticed for the stem cells which ... selectively stains mouse pluripotent stem cells only As such it was applied in mouse abdominal fats to isolate out any potential pluripotent- like stem cells It was noticed that round stem cell-like colonies...

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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS 3

Danh mục: Cao đẳng - Đại học

... Hochedlinger, and G Q Daley Disease-specific induced pluripotent stem (iPS) cells 2008 Cell 134(5): 877–886 9) N Maherali, K Hochedlinger Guidelines and Techniques for the Generation of Induced Pluripotent ... mechanisms and functions can be deduced This will hence give a clearer picture on the compound staining and interaction with the stem cells and a better understanding of what is resulting in pluripotent ... analysis and reculture Since the amount of pluripotent stem cells present in the body are very minimal, CDy9 may therefore aid in isolating just one pluripotent stem cell from the body and this...

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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS 1

Danh mục: Cao đẳng - Đại học

... function (1 - 3) Typically, stem cells can be broadly classified into main groups, the embryonic stem cells and the somatic or adult stem cells The latter, adult stem cells are found in various ... embryonic stem cells (hESC) Until recently, another type of stem cells known as induced pluripotent stem cells (iPSC) have been made available to overcome these ethical concerns (7) These cells are ... of pluripotent stem cells These EBs were allowed to differentiate for days so that they consist of both pluripotent cells and differentiated cells These cells were seeded in 384 well plates and...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc

Danh mục: Sức khỏe giới tính

... sources of pluripotent stem cells These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... The Past, Present and Future of Induced Pluripotent Stem Cells 351 Koji Tanabe and Kazutoshi Takahashi Chapter 19 New Techniques in the Generation of Induced Pluripotent Stem Cells 373 Raymond ... Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine 451 Tammy Laberge and Herman S Cheung Chapter 24 Pluripotent Stem Cells from Testis Sandeep Goel and Hiroshi...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf

Danh mục: Sức khỏe giới tính

... sources of pluripotent stem cells These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... The Past, Present and Future of Induced Pluripotent Stem Cells 351 Koji Tanabe and Kazutoshi Takahashi Chapter 19 New Techniques in the Generation of Induced Pluripotent Stem Cells 373 Raymond ... Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine 451 Tammy Laberge and Herman S Cheung Chapter 24 Pluripotent Stem Cells from Testis Sandeep Goel and Hiroshi...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Danh mục: Báo cáo khoa học

... X and OP at day 3, IHH and ALP at day and RUNX2 at day 14 compared with Ad.GFP controls (Figure 7) These results suggest that BMP-2 and BMP-4 gene transfer induced a significant chondrogenic and ... and AR50249 from to National Institute of Arthritis and Musculoskeletal and Skin Diseases to SCG and CHE, by grant STE 1051/2-1 from the Deutsche Forschungsgemeinschaft (DFG) to AFS and UN, and ... label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, 6-CFDA negative), and viable cells (Ann5...

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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Danh mục: Báo cáo khoa học

... plates and grown to confluence Osteogenesis and adipogenesis were induced as described previously [29] and [30], respectively) Anti-CD3 -induced cell proliferation CD4 + T cells and accessory cells ... Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, were ... proliferation of CD4+ T cells in vitro (a) CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml anti-CD3 antibody and the indicated numbers of mitomycin c-treated...

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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders

Danh mục: Tổng hợp

... 1.1 Stem cells 1.1.1 Human pluripotent stem cells 1.1.2 In vitro differentiation potential of human pluripotent and neural stem cells 1.2 Candidate ... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... 37°C, 5% CO2 and saturated air humidity In vitro differentiation of hPS cells (hES and iPS cells) into long term proliferating pluripotent stem cell-derived neuroepithelial stem (lt-NES) cells was...

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Tiểu luận glycome chẩn đoán của con người induced cells pluripotent stem sử dụng lectin microarray

Danh mục: Y khoa - Dược

... normal- trung bình (25) Tái lập trình yếu tố chép gây Dẫn đến toàn cầu đảo chiều xuống cho Nhà nước Pluripotent Cellular Glycome Cấp Well-Sử dụng lectin microarray phát triển, phân tích 135 mẫu tế...

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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Danh mục: Y học thưởng thức

... searchers have transplanted the bone marrow stem cells into the necrotic femoral heads, and results show bone marrow stem cells can remove vascular lesions and promote angiogenesis in necrotic femoral ... [19-21] Recently, in order to improve the efficacy of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng ... expressed in injured tissues play important roles (2) Stem cells circulate among tissues, and stem cells migrate to the injured tissues once injury occurs Stem cell homing is a complicated process in...

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

Danh mục: Báo cáo khoa học

... mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antiﬁbrotic effect of transplanted mesenchymal stem ... mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell Res ... & Chen X (2008) Lysophosphatidic acid protects mesenchymal stem cells against hypoxia and serum deprivation -induced apoptosis Stem Cells 26, 135–145 21 Li Z, Wei H, Liu X, Hu S, Cong X & Chen...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Danh mục: Báo cáo khoa học

... structure modiﬁcations and factor recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and deﬁnitive erythroid cells (days and 12, respectively) ... transcribed in uninduced murine ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human ... Levings et al density of 105 cells well)1 The cells were passaged again and reseeded on day On days 0, 5, 8, 10 and 12, cells were collected and subjected to RT-PCR and ⁄ or ChIP analysis Globin...

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