ns cell derivation from es cells
Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... post-transcriptional repression 29 Figure 3.1 Cells from neurospheres derived from hESC show cellular characteristics of NSCs 56 Figure 3.2 Cells from neurospheres derived from hESC express neuroectodermal ... whereas in humans it is uniformly expressed in early neuroectodermal cells (cells from which neural progenitors and all other neural cell types originated) derived from fetuses and from hESCs (Pankratz ... miR-145 regulates SOX2 expression in hESCs and glioma cells, and indicates that miR-145 modulates the transition from multipotency to fate commitment in NSCs Together, this study demonstrates a facile...
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Báo cáo khoa học: Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx
... conserved His residues, two conserved Glu residues, and five conserved Asp residues As His, Cys, Glu, and Asp are often ligands for zinc ions [8], the aforementioned conserved aminoacid residues ... aminopeptidase activity These results suggest that aspartyl aminopeptidases are present in several eukaryotes Basic amino acids were conserved at several sites of the aspartyl aminopeptidases One site, indicated ... sequences are shaded, and histidines, glutamates, and aspartates conserved among all sequences are indicated with * The sites of basic amino acids conserved among all of the aspartyl aminopeptidases...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... named G2 cells These cells and logarithmically growing cells were separated into Triton-soluble (S) and Triton-insoluble (P) fractions After electrophoresis, proteins in these fractions were analyzed ... by western blot analyses (A) Logarithmically growing HeLa cells were incubated with nocodazole Cells detached from the bottle by shaking were collected and named mitotic (M) cells Residual cells ... was preserved throughout such treatments; i.e the DNA content of G2, M cells are almost double that of G1 cells and the DNA content of S cells ranges between that of G1 cells and G2 or M cells...
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Báo cáo khoa học: A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons pptx
... at the respective daf stages Freshly harvested seeds were used in the subsequent experiments Chromatographic materials, enzymes, nucleotides, DNA substrates and all other chemicals were from Amersham ... these stages However, aphidicolin-sensitive DNA synthesis at seed maturation stages (19–30 daf) indicates the involvement of replicative DNA polymerases like a and d Detection of expression of ... DNA polymerase carries out moderately processive DNA synthesis The primer was elongated by an average of 35 nucleotides and also showed significant variation in response to changes in reaction condition...
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Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate affinity ppt
... the purity of mast cells thus obtained ranged from 65% to 95% of all nucleated cells Isolation of skin mast cells Mast cells were isolated as described previously from infant foreskin tissue obtained ... epithelial cells [32] In contrast, most preparations of tissue mast cells contain ample mRNA encoding both a- and b-tryptases [35] a-Tryptase appears to be released constitutively from mast cells ... 500 g for 20 at C Cells were pooled from the bottom of the gradient and the 7080% interface These suspensions consisted of 7098% mast cells Enzyme purication Tryptase was puried from high salt extracts...
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báo cáo hóa học:" Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels" docx
... studies about the link between birth weight and the properties of the cells from the UC vessels Our aim has been obtaining the four vascular cell types from each individual UC to determine their cellular ... SMCs cultures covering a 75-cm2 growing area were harvested and aliquots cryopreserved These aliquots were considered to correspond to cells at passage Cellular characterization Cryopreserved ECs ... (DiI)-labeled Ac-LDL as described [16] Cellular senescence Cells were seeded as above, and the percentage of senescent cells was determined as follows Cell culture media was removed from the well and...
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Báo cáo hóa học: " Quantitative expression analysis of HHV-6 cell receptor CD46 on cells of human cord blood, peripheral blood and G-CSF mobilised leukapheresis cells" docx
... hematopoietic from G-CSFcells from cord blood cells [triangles] and molecules on cell CD34+ cells of [circles], leukapheresis products (LP) progenitor mobilised precursor (CB) [squares], peripheral ... CD13 Cell surface antigen Figure angles] products from cord (PB), (CB), [A, and leukapheresis blood of1adult donors blood[B, circles]squares], cells mature leukocytes of from molecules on cell ... mature lymphocytes and myeloid cells, as well as hematopoietic progenitor cells, express CD46 B-lymphocytes express higher levels of CD46 than T-lymphocytes; CD8+ T-lymphocytes exhibit less CD46 than...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with S17 mouse stromal cells to derive cystic bodies Later, purified CD34+ cells derived from ... disease process [14] We previously demonstrated the derivation of macrophagres from hES-CD34+ cells whereas studies of Galic et al [20] derived functional T cells from hES-CD34+ cells in vivo ... could be derived from hES-CD34+ cells Large numbers of these hES-DCs cells can now be cultured from a renewable source for use in cell and immune-based therapies Since these cells also support...
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Trypsin-induced proteome alteration during cell subculture in mammalian cells potx
... adherent -cell- subculture is to detach cells from the substratum as the cells reach high confluence Trypsin is often applied for this purpose Cells are subsequently subdivided and reseeded into fresh ... expressed proteins in MCF-7 cells Protein samples purified from total cell lysates were labeled with Cy dyes and separated using 24-cm, pH 3-10 nonlinear IPG strip The differentially expressed ... purchased from Invitrogen (all from Gibco-Invitrogen Corp., UK) Cell lines and cell cultures The breast cancer cell line MCF-7 and cervical cancer cell line Hela were both purchased from American...
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Báo cáo y học: "Acute myeloid leukemia of donor origin after allogeneic stem cell transplantation from a sibling who harbors germline XPD and XRCC3 homozygous polymorphisms" pps
... Lane = digestion pattern from patient alleles from AML cell DNA after transplantation; Lane = digestion pattern from donor cell alleles post-SCT hematopoietic cells and the donor’s cells were ... patient’s pre- and post-transplantation (AML) cells and donor’s cells Polymorphism DNA repair mechanism Patient cells (pretransplantation) AML cells (posttransplantation) Donor cells XPA A23G NER Homozygous ... non-digested PCR amplification of normal allele; Lane = digestion pattern from the positive control K562 myeloid cell line alleles; Lane = digestion pattern from patient alleles before transplantation;...
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Báo cáo khoa hoc:" Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad" docx
... of HEp cells to 50% of the initial level, and this was chosen as the IC 50 Longer exposures resulted in additional toxicity to the cells These results demonstrate that silver nanoparticles mediate ... pathway of cells, were increased following the treatment with silver nanoparticles The cell lysates obtained from HEp2 cells treated with silver nanoparticles at 500 nM concentrations for six ... and sensors The in vitro cytotoxicity effects of silver nanoparticles were screened against cancer cell lines and viability of tumor cells was confirmed using MTT assay The silver nanoparticles...
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Báo cáo y học: "A novel PTCH1 germline mutation distinguishes basal cell carcinoma from basaloid follicular hamartoma: a case report" pdf
... germline mutations associated with NBCC have been reported, most (73%) identifying nonsense or frameshift mutations leading to the synthesis of a truncated PTCH protein [5] These mutations appear to ... dermis contains nests of basaloid tumor cells with peripheral palisading nuclei surrounded by mucinous stroma (E) BCL-2 immunostaining illustrates focal brown staining of the tumor cells Many of ... superficial dermis These nests were characterized by proliferation of basaloid cells with palisading nuclei at the periphery of the nests The stroma around the tumor had loose mucinous changes and retraction...
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Báo cáo y học: " Mometasone and desloratadine additive effect on eosinophil survival and cytokine secretion from epithelial cells" docx
... epithelial cell purity was 98.9 ± 0.1% for NM and 92.3 ± 1.3% for NP specimens Culture of epithelial cells Epithelial cell suspensions (105 cells/ well) were placed on 24-well plates coated with ... epithelial cells but also in other cell types On this regard, DL decreased IL-6 and IL-8 secretion from basophilic cells (KU812) and human mast cell line (HMC-1) [33], IL-4 and IL-13 from basophil-enriched ... expression in murine blood cells [18] as well as the ICAM-1 expression in skin [31] and lung fibroblasts [32] In the present study, DL inhibited IL-6 secretion from both NM and NP epithelial cells...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... neural crest cells and their lineages by BMP2 promoter-driven selection markers paves the way for ES cell- derived neural crest stem cell biology These cells are attractive candidates for future cell ... 5a,e) This suggests that cardiomyogenesis is repressed in the BMP2+ cells Accordingly, Mesp1 was repressed (Figure 2a) EBs prepared from a mixture of BMP2+ cells with wild-type ES cells in ratios ... adipocytes, these genes are also expressed in macrophages Similarly, the trophoblast lineage also expresses c-fms [82] Notably, the cells derived from the BMP2+ cells cultured in the continual presence...
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Báo cáo y học: " From single cells to whole organism" pps
... signaling connections as animals became more complex Signaling that leads to the induction of new gene expression enables cells to adapt to environmental changes or, in the case of cell- cell communication, ... a cellular level, whereas its overexpression increases the protective effect Even if it is still a long way from bench to bedside, this is a clear example of the ongoing translation from research ... development was presented by Irma Theseleff (University of Helsinki, Finland) in the context of tooth development in mice The development of these ectodermal appendages starts from tooth placodes (thickened...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... KG-1 cells comment Stimulate with PMA + ionomycin for 24hrs PBMCs CD19+ve cells = waste CD19 depletion DC-like KG-1 cells (immature) CD19 –ve cells reviews CD1c selection CD1c-ve cells CD14-ve cells ... interactions Figure Cytokine expression profiles of cells Cytokine expression profiles of cells Cytokine production by mDCs and the DC models in response to LPS Cytokine concentrations were measured ... Phenotypic comparison of cells Phenotypic comparison of cells The expression of cell surface markers on (a) immature and (b) mature mDCs, moDCs and KG1 cells Immature cells were freshly isolated mDCs,...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... T-test 3.2.9 in vitro sensitivity of TK neural stem cell (NSCtk) to GCV To test sensitivity to Ganciclovir (GCV), the obtained NSCtk transduced cells and untransduced NSCs were cultured in the NSC ... aggregates that can be harvested at 90 x g for 5min This step removes debris and dead cells, enriching for NSC founders, and ensures complete media exchange Cells can then be re-plated in fresh NS ... derived NSCs from human iPSCs are based on methods similar to that of NSCs derived from hES cells (Zhao et al., 2011) Prior to differentiation of iPS cells into NSCs, iPS cell colonies cultured...
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Development of a MACS based strategy for isolating rare cell populations from animal tissue for transcription factor studies
... manipulations ensure high cell viability The enrichment of the cells of interest reduces the amount of undesired cells in culture than may overgrow rare cell populations Often the isolated cells are ... expression profiles (Sievertzon, Wirta et al 2005) Many studies have compared gene expression profiles from primary cell cultures, established immortal cell lines and tissues To study hepatocytes, ... of in vivo processes studied 2.1.1 Whole organs, tissues or biopsies 7 2.1.2 2.1.3 Primary cell cultures 16 2.1.4 Established immortal cell lines 18 2.1.5 Fluorescence Activated Cell Sorting (FACS)...
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Robust detection and classification of biomedical cell specimens from light microscope images
... assessment of these specimens enables us to undertake preventive measures which could potentially save millions of lives The practice of identifying specimens of interest from microscope images even ... cancer cells [93], plant cells [21], chromosomes [59], and crushed aggregates [91], to name a few However, tests conducted by Wang [91] and experimental results presented in Section 4.8 of this thesis ... into the constituent cells The rule set ensures that (1) valid concavities are effectively distinguished from minor boundary irregularities, (2) concavity regions at the ends of split lines are suitable...
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Homologous recombination and directed differentiation in medaka ES cells development of vector systems
... No Yes Yes Crossing in lab Yes Yes No Linkage map Yes Yes No Information on sequenced Much Much Much None Good None Many Yes Yes No Transgenic fish Yes Yes No Chimeric fish Yes Yes No Gynogenesis ... melanocytes from medaka ES cell As stated earlier, one of the most striking properties of ES cells is the ability to differentiate into virtually all cell types This property makes ES cells a ... Yes Yes No ES- like cells No Yes No No Yes No genes, mapped genes and DNA markers Genetic information on wild populations The number of inbred strains Cryopreservation of spermatozo Active transposable...
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