npt for protein delivery
The evaluation of bioactive polycaprolactone scaffolds as protein delivery systems for bone engineering applications
... thesis showed for the first time that threedimensional, bioactive polycaprolactone scaffolds can serve effectively as protein delivery systems for bone regeneration and has the potential for clinical ... effective protein delivery systems for the repair 28 of bone tissue The merit of the work was that the PCL-based scaffolds could serve not only as a template for bone growth but also as a delivery ... rhBMP-2 for use in spinal fusion procedures Given the unequivocal evidence for a role of BMP-2 in bone development, it was adopted for our experiments The major requirements of a delivery system for...
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POE and POE PEG POE triblock copolymeric microspheres for controlled protein delivery
... in Selecting A Polymer for Controlled Drug Delivery 40 2.10 Protein Delivery and A Model Protein - Bovine Serum Albumin (BSA) 2.11 41 Future Directions of Controlled Drug Delivery 43 2.12 Research ... where controlled delivery is actually an enabling technology for a drug For example, the drug is unstable or highly toxic without the controlled delivery devices The most active areas for development, ... microspheres formation process Figure 4.2 Pore forming process during the first 30 minutes of microspheres formation (POE-PEG(5%)-POE microspheres) Figure 4.3 Diameter change during the microspheres formation...
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Tài liệu KRONE - Catalogue - HighBand - For Broadband delivery - HK- Jun 2000 pdf
... Test Cord Used for testing HIGHBAND 10 circuits Product Number: 6451 100-30 3M Pack Qty: Bag of Please contact KRONE for further information on the full range of Test/Patch cords for the HIGHBAND ... Connectivity” for Broadband Delivery Individual pairs closer together Adjacent pairs higher seperation Less metallic cross sectional area reducing capacitance The Result The World’s Highest Performing ... telecommunications suppliers to lay the foundation for public access communications infrastructure on both copper and fibre optic cabling architectures Enabling the delivery of both today’s and tomorrows...
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Tài liệu KRONE AccessNET - Opens the Pipe for Broadband Delivery doc
... central office/CBD Customer Delivery Transport Delivery Customer • Broadband Delivery Solutions, ie local loop • Subscriber Interface Solutions KRONE : Your Partner for Quality and the Finest ... Internet Access or combinations of these and other services, the ‘Speed for Need’ is critical To satisfy this need for speed the delivery infrastructure has to be resilient, cost effective and bandwidth ... SUBSCRIBER INTERFACE SOLUTIONS Products for both residential and commercial subscribers that solve issues around Network Termination Points, receiving and managing information via POTS, ISDN, xDSL, etc...
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Tài liệu Báo cáo khoa học: Cell-free translation systems for protein engineering docx
... a strong tool for producing proteins Production of biologically active proteins In order for the cell-free translation system to produce biologically active proteins, additional proteins such ... strategies for efficient synthesis of biologically active proteins Correct disulfide bond formation in proteins such as antibodies can be facilitated by the addition of the redox-dependent chaperone protein ... ribosome, to form a link consisting of protein ⁄ tRNA ⁄ ribosome ⁄ mRNA In the in vitro virus or mRNA display, a covalently linked Cell-free translation for protein engineering mRNA ⁄ puromycin ⁄ protein...
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REQUIREMENTS FOR PROTEIN MEALS FOR RUMINANT MEAT PRODUCTION IN DEVELOPING COUNTRIES pot
... arises for immature animals on poor quality forage, to what extent can growth be increased by providing nutrients for the rumen and extra protein for absorption? The provision of more protein for ... demand for food for humans and feed for livestock Grain for livestock, will have to compete with increasing demand for grain to produce industrial alcohol The latter arises from the demand for industrial ... obvious associated errors Response to escape protein of young ruminants given low digestibility forage Credit for the discovery of the need for escape protein in the diets of producing ruminants...
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Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
... The amplifier gain was set at 1.00 for forward scattering; the detector voltage was set to E00 for forward scattering and 600 V for FL1, and the threshold for forward scattering was set at 52 The ... based on G -protein signaling for detection of protein protein interactions, using a mutated Gc that lacks membrane-localization ability Our assay can be performed under conditions suitable for maximal ... was to establish a rapid and reliable method for the detection of protein protein interactions using yeast G -protein signaling In our system, protein protein interactions were detected utilizing...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... determinants for the susceptibility of the protein to protein N-myristoylation For postgenomic studies, reliable tools for the prediction of co- and posttranslational modifications would be valuable for ... complete substrate protein, at least the N-terminal 17 residues of the substrate protein experience amino acid type variability restrictions for protein N-myristoylation [33] Therefore, it might ... N-myristoylated proteins having Lys residue at position Amino acids at positions 3, and are in bold type Protein Ca2+ binding/EF hand proteins Aplycalcin Hippocalcin Neurocalcin Rem-1 Visinin-like protein...
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Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx
... GCATATGGCAGCTGCG for the 5¢ end of mAK-L; 5¢-GTAGGTGCACATATGACGTCCACC for the 5¢ end of mAK-S; 5¢-ATATAGGATCCTCAGTGGAAGTC TGG for the 3¢ end of both clones Consensus PKC phosphorylation sites were selected for ... physiological substrates for each protein kinase Specifically, protein phosphatase inhibitor-1 was used in the PKA, MAPK, Cdk1 and Ó FEBS 2004 Recombinant mouse AK as a protein phosphorylation ... long and short isoforms of the enzyme Many of the tissues included in this analysis also showed a prevalence of one isoform over the other For instance in the spleen, the short isoform is the predominant...
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Báo cáo khoa học: ThermoFAD, a ThermofluorÒ-adapted flavin ad hoc detection system for protein folding and ligand binding pdf
... ThermoFAD for various flavoproteins The selected flavoproteins differ with respect to the type of flavin cofactor, flavin linkage to the protein, and source organism of the protein (for details see Table ... detection system F Forneris et al Table Comparison of unfolding temperature using ThermofluorÒ and ThermoFAD for various flavoproteins ND, not determined Protein Organism Bound flavin Tm (°C) Protein concentration ... cofactors in flavoproteins is usually quenched by the protein environment when the protein is properly folded [16], we realised that is possible to measure the unfolding temperature of a flavoprotein using...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... cytofectins for gene delivery H3C H3C N CH3 N H3C N O O O NH O NH R R The R group varies with the derivative: C11H23 for dilauroyl (1,3lb1) C13H27 for dimyristoyl (1,3lb2) C15H31 for dipalmitoyl ... parameter is equal to for a univalent base (and -1 for a monoprotic acid) The pKa values listed in Table for the bivalent lipids are the mean of two acid dissociation constants, one for each of the ... except for formulations containing 1,3lb1, which were poorly tolerated (data not shown) FEBS Journal 275 (2008) 148162 ê 2007 The Authors Journal compilation ê 2007 FEBS 151 Novel cytofectins for...
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Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
... of Cry4Aa facilitated the formation of protein inclusions of fused heterologous protein in E coli Therefore, the block sequence may be one of the factors responsible for the crystallization of ... 4AaCter 696–851 efficiently formed protein inclusions in E coli, and the fusion protein solubilized from the inclusions had the highest GST activity among the tested samples Therefore, it was most probable ... [1] Therefore, the C-terminal extension is thought to contain important elements responsible for protein crystallization In addition, the cysteines located in the C-terminal extension may form intermolecular...
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Báo cáo khoa học: and protein bilinear indices – novel bio-macromolecular descriptors for protein research: I. Predicting protein stability effects of a complete set of alanine substitutions in the Arc repressor ppt
... descriptors for protein research I Predicting protein stability in Arc repressor mutants PROTEINS: Structure, Function, and Bioinformatics 56, 715723 35 Gonzalez-D az H & Uriarte E (2005) Proteins ... bilinear indices for the example protein (Tables and 3) is shown in Table Any local protein bilinear index has a particular meaning, especially for the rst values of k, where the information about ... values of tm for cases in both training and test sets Different protein folding may be the reason for the lack of linear correlation between protein bilinear indices and stability (tm) for these...
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computational methods for protein folding
... appropriate for modeling a helix-coil transition than protein folding Nevertheless, as will be discussed below, native structure does play a role for certain lattice models [10,11] as it does for proteins ... standard deviation for a given descriptor was 0.03 for rtrn and 0.06 for rcv As stated above, the coefficients denoted ‘‘trn’’ are for results obtained with networks trained on all 33 proteins; in other ... order for these proteins is somewhat surprising because no significant correlation was observed in a previous study of a database of 24 proteins [14], 19 of which are included here For those 19 proteins...
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nanoparticle technology for drug delivery, 2006, p.427
... Chapter for injectable delivery, Chapter for oral delivery, Chapter 10 for brain delivery, Chapter 11 for ocular delivery, and Chapter 12 for gene delivery Finally, the fourth section provides an ... drug delivery Depending on the route and purpose of drug delivery, the requirements for nanoparticulate systems can vary These aspects are discussed in Chapter for injectable delivery, Chapter for ... Microparticulate Systems for the Delivery of Proteins and Vaccines, edited by Smadar Cohen and Howard Bernstein 78 Good Manufacturing Practices for Pharmaceuticals: A Plan for Total Quality Control,...
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Báo cáo toán học: " Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage" pdf
... desiccator for at least h before weighing Selected samples were frozen at −20 °C and lyophilized for 48 h The effects of pH and salt on protein extraction The amount of liquid used for protein extraction ... °C) to form a discrete protein (micelle) in the aqueous phase The protein micelle was allowed to settle to form an amorphous, gelatinous mass The protein mass was centrifuged at 10,000 rpm for 10 ... estimate protein concentration (%) by means of a nitrogen-to -protein conversion factor 5.7 (Sosulski et al 1990) for TS and 5.5 (Lindeboom and Wanasundara 2007) for mustard seed, meal, and protein...
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Báo cáo hóa học: " Efficient data replication for the delivery of highquality video content over P2P VoD advertising networks" pdf
... system is based on the best-effort delivery service in the form of UDP (the size of the UDP datagram is limited to 1,500 bytes), which does not guarantee reliability or the delivery order of network ... such as a high advertisement delivery rate, low advertisement delivery time, and a flood of advertisement messages However, the instant advertising method is suitable for limited or specific spatial/locational ... server) for the delivery of advertisement messages to the target audience It depends even more on dynamic sharing-peers delivering advertisements during video sessions We evaluate the performance...
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Báo cáo hóa học: " Functionalized halloysite nanotube-based carrier for intracellular delivery of antisense oligonucleotides" pdf
... http://www.nanoscalereslett.com/content/6/1/608 showed the cellular delivery efficiency of f-HNTASODNs estimated to be 98.69%, indicating that the fHNTs had high intracellular delivery ability for ASODNs Therefore, f-HNTs could be effective ... delivery, bioimplants, and for protective coating (e.g., anticorrosion or antimolding) [19-23] Despite these prospects, however, their utilization as biocarrier for ASODNs delivery has been less ... laser for FAM excitation at 488 nm was used for imaging, and an oil immersion objective (Plan Apo, SEIWA OPTICAL AMERICA INC., Santa Clara, CA, USA; magnification = 63 × 1.4) was used for cellular...
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Báo cáo hóa học: "Preparation and evaluation of novel mixed micelles as nanocarriers for intravenous delivery of propofol" potx
... CLE before the first 12 h, the release rate of the two formulations was comparable thereafter Overall, quick release rate for mixed micelles might produce a favorable pharmacological effect for ... micelles and CLE, separation of the two phases was performed using ultracentrifugation Ultracentrifugation was performed at 10,000 rpm (14000 g) for 20 Amicon ultra centrifugal filter units (Amicon ... presented in Figure Time for righting reflex recovery was 7.17 ± 2.75 and 7.29 ± 1.25 min, for mixed micelles and CLE, respectively On average, both groups of animals given propofol formulations recover...
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báo cáo hóa học:" Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage" docx
... (4°C) to form a discrete protein (micelle) in the aqueous phase The protein micelle was allowed to settle to form an amorphous, gelatinous mass The protein mass was centrifuged at 10,000 rpm for 10 ... estimate protein concentration (%) by means of a nitrogen-to -protein conversion factor 5.7 (Sosulski et al 1990,) for TS and 5.5 (Lindeboom and Wanasundara 2007) for mustard seed, meal, and protein ... efficiency of protein extraction or affect the quality of the extracted protein Neither the efficiency of protein extraction nor the quality of protein was affected by the whole stillage Therefore, we...
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