npt for dna delivery
Báo cáo sinh học: " A new plasmid vector for DNA delivery using lactococci" pptx
... implement safer strategies for DNA deliver and expression into eukaryotic cells, we reinforce the use of Lactococcus lactis as DNA delivery vehicle [18,26] To improve our delivery DNA strategy, we constructed ... per milliliter): for E coli, erythromycin (100) and chloramphenicol (10); for L lactis, erythromycin (5) and chloramphenicol (10) DNA manipulations DNA manipulations were performed as described ... been performed to examine whether these strains are able to release enough DNA to ensure an efficient intestinal cell expression in vivo In long term, an alternative strategy for DNA vaccine delivery...
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Tài liệu KRONE - Catalogue - HighBand - For Broadband delivery - HK- Jun 2000 pdf
... Test Cord Used for testing HIGHBAND 10 circuits Product Number: 6451 100-30 3M Pack Qty: Bag of Please contact KRONE for further information on the full range of Test/Patch cords for the HIGHBAND ... Connectivity” for Broadband Delivery Individual pairs closer together Adjacent pairs higher seperation Less metallic cross sectional area reducing capacitance The Result The World’s Highest Performing ... telecommunications suppliers to lay the foundation for public access communications infrastructure on both copper and fibre optic cabling architectures Enabling the delivery of both today’s and tomorrows...
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Tài liệu KRONE AccessNET - Opens the Pipe for Broadband Delivery doc
... central office/CBD Customer Delivery Transport Delivery Customer • Broadband Delivery Solutions, ie local loop • Subscriber Interface Solutions KRONE : Your Partner for Quality and the Finest ... Internet Access or combinations of these and other services, the ‘Speed for Need’ is critical To satisfy this need for speed the delivery infrastructure has to be resilient, cost effective and bandwidth ... SUBSCRIBER INTERFACE SOLUTIONS Products for both residential and commercial subscribers that solve issues around Network Termination Points, receiving and managing information via POTS, ISDN, xDSL, etc...
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Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf
... Therefore, systemic and extensive investigations are still required to assign universally appropriate parameter sets of the temperature-dependent thermodynamics for the DNA/ DNA and RNA /DNA oligonucleotide ... should be noted that for short oligonucleotide sequences, the duplex formation behaves in a two-state transition [17,43], while for longer oligonucleotide sequences, the duplex formation often behaves ... requires that the melting tempera- Fig Heat capacity change vs the number of base pairs for DNA/ DNA (s) and RNA /DNA (d) oligonucleotide duplexes tures of all the investigated sequences should have...
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Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf
... range of movements for the structured helices a1 that are necessary for nonspecific DNA target recognition From these data, it is possible to propose a significant role in DNA binding for the loop connecting ... the different structural and conformational features of the spacer regions in the DNA target sequences, indicate diverse mechanisms for the recognition of the DNA Results Analysis of root-mean-square ... nonspecific DNA sequences For both the E2s, we suggest that the ‘indirect readout’ [30] plays a significant role in DNA sequence recognition, although a systematic rational perturbation of the DNA- binding...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... cytofectins for gene delivery H3C H3C N CH3 N H3C N O O O NH O NH R R The R group varies with the derivative: C11H23 for dilauroyl (1,3lb1) C13H27 for dimyristoyl (1,3lb2) C15H31 for dipalmitoyl ... parameter is equal to for a univalent base (and -1 for a monoprotic acid) The pKa values listed in Table for the bivalent lipids are the mean of two acid dissociation constants, one for each of the ... compilation ê 2007 FEBS 153 Novel cytofectins for gene delivery M Spelios and M Savva Cationic lipidpDNA binding studies Binding curves of EtBr-intercalated pDNA titrated with aliquots of cationic lipid...
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Báo cáo khoa học: Helicobacter pylori neutrophil-activating protein activates neutrophils by its C-terminal region even without dodecamer formation, which is a prerequisite for DNA protection – novel approaches against Helicobacter pylori inflammation ppt
... Fe2+ for 15 Lane 2: DNA with HPNAPwt, exposed to Fe2+ for 15 Lane 3: DNA exposed to 0.5 mM Fe2+ for 30 Lane 4: DNA with HP-NAPwt, exposed to Fe2+ for 30 Lane 5: DNA exposed to 0.5 mM Fe2+ for ... for 45 Lane 6: DNA with HP-NAPwt, exposed to Fe2+ for 45 Lane 7: DNA exposed to 0.5 mM Fe2+ for 60 Lane 8: DNA with HP-NAPwt, exposed to Fe2+ for 60 Lane 9: nonexposed DNA Lane 10: DNA exposed to ... (C) Lane 1: plasmid DNA Lane 2: DNA incubated with 0.5 mM Fe2+ for 60 Lane 3: DNA incubated for 60 with Fe2+-loaded HP-NAP Lanes and show DNA incubated with 0.5 mM Fe2+ and DNA incubated with...
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nanoparticle technology for drug delivery, 2006, p.427
... of drug delivery, the requirements for nanoparticulate systems can vary These aspects are discussed in Chapter for injectable delivery, Chapter for oral delivery, Chapter 10 for brain delivery, ... Chapter 11 for ocular delivery, and Chapter 12 for gene delivery Finally, the fourth section provides an overview of the clinical, ethical, and regulatory issues of nanoparticle-based drug delivery ... Supercritical Solution for Particle Formation 59 RESS with Solid Cosolvent for Nanoparticle Formation 63 Supercritical Antisolvent Process for Particle Formation 66 SAS with Enhanced...
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báo cáo hóa học: " A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells" pot
... stranded B -DNA This molecule is a synthetic DNA that displays a helical B-form configuration in solution Therefore, poly(dA:dT) double stranded B -DNA resembles the form of HSV-1 genomic DNA in infected ... which glia recognize neurotropic DNA viruses such as HSV-1 and elicit inflammatory CNS damage Replicating DNA viruses generate genomic DNA that serves as a ligand for DAI DAI then associates with ... whole cell protein isolates were collected for analysis For comparison purposes, cells were exposed to transfection reagent alone or HSV-1 (MOI of and 10) for 24 hours Quantification of IL-6 and...
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Báo cáo hóa học: " Efficient data replication for the delivery of highquality video content over P2P VoD advertising networks" pdf
... system is based on the best-effort delivery service in the form of UDP (the size of the UDP datagram is limited to 1,500 bytes), which does not guarantee reliability or the delivery order of network ... such as a high advertisement delivery rate, low advertisement delivery time, and a flood of advertisement messages However, the instant advertising method is suitable for limited or specific spatial/locational ... server) for the delivery of advertisement messages to the target audience It depends even more on dynamic sharing-peers delivering advertisements during video sessions We evaluate the performance...
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Báo cáo hóa học: " Functionalized halloysite nanotube-based carrier for intracellular delivery of antisense oligonucleotides" pdf
... cellular delivery efficiency of f-HNTASODNs estimated to be 98.69%, indicating that the fHNTs had high intracellular delivery ability for ASODNs Therefore, f-HNTs could be effective in transporting DNA ... delivery, bioimplants, and for protective coating (e.g., anticorrosion or antimolding) [19-23] Despite these prospects, however, their utilization as biocarrier for ASODNs delivery has been less ... laser for FAM excitation at 488 nm was used for imaging, and an oil immersion objective (Plan Apo, SEIWA OPTICAL AMERICA INC., Santa Clara, CA, USA; magnification = 63 × 1.4) was used for cellular...
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Báo cáo hóa học: "Preparation and evaluation of novel mixed micelles as nanocarriers for intravenous delivery of propofol" potx
... CLE before the first 12 h, the release rate of the two formulations was comparable thereafter Overall, quick release rate for mixed micelles might produce a favorable pharmacological effect for ... micelles and CLE, separation of the two phases was performed using ultracentrifugation Ultracentrifugation was performed at 10,000 rpm (14000 g) for 20 Amicon ultra centrifugal filter units (Amicon ... presented in Figure Time for righting reflex recovery was 7.17 ± 2.75 and 7.29 ± 1.25 min, for mixed micelles and CLE, respectively On average, both groups of animals given propofol formulations recover...
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báo cáo hóa học:" Research Article Impact of Missing Value Imputation on Classification for DNA Microarray Gene Expression " ppt
... using cDNA microarray containing 26,260 different genes [35] In addition to the log-ratio gene expression data, additional information such as background (foreground) intensities and SD of foreground ... harder for the imputation algorithms to estimate the MVs In addition, it is observed that the classification performance of one imputed dataset may outperform that of the EURASIP Journal on Bioinformatics ... classification performances of imputed datasets depend on the underlying combination of feature selection method and classification rule For example, RAVG and KNNimpute show satisfactory performances for the...
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Báo cáo hóa học: "Single-component Polymer Nanocapsules for Drug Delivery Application" docx
... cells, and delivery of the anticancer drug into the tumor cells, leading to tumor cell death Bansal notes that it is highly desirable for antitumor applications, that the size of the delivery vehicle ... will further provide functional capsules for targeted drug delivery applications 267 Overall, the simple, efficient, and general nature of the approach for the fabrication of a new class of monodispersed, ... mM carboxypeptidase) with a nearlinear drug release kinetics over the first 24 h ‘‘Moreover, for a drug delivery vehicle to be highly effective, it is desirable that it should not degrade in the...
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Báo cáo toán học: "Bounds for DNA codes with constant GC-content" pdf
... Our bounds for DNA codes with constant GC-content correspond to the partition {0, 1, 2, 3} = {0, 3} ∪ {1, 2} Halving bound Any upper bound for AGC (n, d, w) yields an upper bound for AGC,RC (n, ... halving bound for DNA codes with unrestricted GCcontent in [17] The same proof works here, since the reverse-complement of a DNA word has the same GC-content as the word itself Theorem For < d ≤ ... Gilbert bound for q-ary codes (see e.g [20]), and a similar argument applies to constant-weight codes (see e.g [4]) Here we give an analogue for DNA codes with constant GC-content: Theorem For ≤ d...
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Báo cáo khoa học: "Statistical analysis of IMRT dosimetry quality assurance measurements for local delivery guideline" ppt
... desired for the dose distribution, created optimized fluence distributions for a number of pre-selected beams, and then transformed them into dynamic MLC movements [26] The calculation grid used for ... (s):-0.55 ± 1.51) for the brain case, from -4.6% to +2.7% (-1.62 ± 1.23) for the H&N case, and from -4.6% to +2.5% (-0.41 ± 1.21) for the abdominal or prostate case The confidence limits for each treatment ... mm for 2D dose analysis of composite plans The percentage of points passing the gamma criteria was on average 95.2 ± 7.4% for the H&N case, 97.1 ± 3.1% for the brain case, and 99.7 ± 0.5% for...
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Báo cáo y học: "Magnetically retainable microparticles for drug delivery to the joint: efficacy studies in an antigen-induced arthritis model in mice" doc
... per group, Page of 10 (page number not for citation purposes) was necessary for at least two reasons First, there was a need to perform these control tests for the correct evaluation of the action ... coagulate for at least 30 minutes prior to centrifugation at 4,000 revolutions per minute to collect the serum The knees were dissected, fixed with 4% formaldehyde in PBS and used for histological ... investigated magnetically retainable drug delivery systems, an approach as yet clinically unexploited despite the intense need for the development of novel i-a delivery modalities Thus, our aim was...
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báo cáo khoa học: "Optimization of DNA delivery by three classes of hybrid nanoparticle/DNA complexes" pptx
... added to form Emulsion (W/O/W) The mixture was briefly sonicated and NPs were formed by solvent evaporation For DNA encapsulated NPs (in and both), pLuc DNA was added to solution I For DNA adsorbed ... seen for NPs with encapsulated DNA (in) (Figure 2) Optimization of NP /DNA complex delivery conditions to 293 cells We compared the efficiency of DNA delivery to 293 cells by the six NP /DNA complexes ... than DNA alone for luciferase gene delivery (500-600 times more efficient) A dose response curve for gene delivery of six different NP /DNA complexes to 293 cells has been generated; optimal delivery...
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Báo cáo khoa hoc:" Evaluation of triblock copolymeric micelles of δvalerolactone and poly (ethylene glycol) as a competent vector for doxorubicin delivery against cancer" doc
... nanometer range with a low polydispersity Also the low CMC value for micelle formation suggests that VEV can be a good nanocarrier for drug delivery Preparation and properties of DOX loaded copolymeric ... and VEV as a carrier for future applications in drug delivery Conclusions A novel δ-valerolactone and PEG based triblock copolymer was synthesized and characterized for drug delivery applications ... for 24 h Cells were harvested and fixed with 70% ethanol for h The fixed cells were then given RNAse A (100 mg/ml) treatment for h at 37°C followed by propidium iodide (10 mg/ml) incubation for...
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Báo cáo khoa hoc:" Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity" potx
... transfection efficiency for plasmid DNA delivery into cells is still an important issue in gene therapy Thus, a number of different approaches have been used to increase efficiency [10-12] Unfortunately, ... described above Transfection was performed as explained for the FHD protocol Subsequently, 24 h after transfection cells were fixed in a 4% paraformaldehyde/PBS solution for 20 minutes at room temperature, ... plasmid DNA carriers and transfection enhancers Similarly, magnetic NPs loaded with the nucleic acid of interest are used to increase transfection efficiency by applying magnetic force to the DNA- NP...
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