notch pdgf hedgehog gli1 and bone morphogenetic protein signalling pathways implications in control of cd133 csc function in gliomas
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or 25 ng/mL BMP-2, or 25 ng/mL BMP-4 (all R&D Systems, Minneapolis, ... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral ... similar pattern of hypertrophy and apoptosis was observed in the untransduced control aggregates that were maintained in the presence or absence of recombinant BMP-2, BMP-4 or TGF-β1 protein (not shown)...
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báo cáo hóa học:" Expression of Bone Morphogenetic Protein-2 in the Chondrogenic and Ossifying Sites of Calcific Tendinopathy and Traumatic Tendon Injury Rat Models" doc
Ngày tải lên :
20/06/2014, 04:20
... expression of BMP-2 protein and mRNA in both animal models BMP-2 protein was detected in the chondrocyte-like cells and calcific deposits in both injury models but not in control samples, indicating ... member of the family and has been used in many studies for augmentation of bone and bone- tendon junction regeneration [3,4] Because of the role of BMP-2 in bone formation, it is commonly found in bone ... traumatic induced Immunohistochemical staining of BMP-2 in collagenaseImmunohistochemical staining of BMP-2 in collagenase-induced calcific tendinopathy and central one-third traumatic injury model...
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Báo cáo y học: "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling" pps
Ngày tải lên :
09/08/2014, 10:21
... VDIPEN staining (k) and NITEGE staining (q,p) Arrows point to intense staining around chondrocytes FastG, fast green; SafO, safranin O Co-incubation of several dilutions of BMP-2 protein with the ... differences in safranin O staining intensity between BMP-2-exposed cartilage and controls were observed by mere visual investigation Therefore, the safranin O staining intensity was scored in patellar and ... imaging system There was a significant (30%) increase in safranin O staining intensity in the patella on day When the data of all time points were pooled, a significant increase in safranin O...
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Báo cáo y học: "Dynamic activation of bone morphogenetic protein signaling in collagen-induced arthritis supports their role in joint homeostasis and disease" ppsx
Ngày tải lên :
09/08/2014, 13:22
... Bone morphogenetic proteins in bone tumors J Orthop Sci 2004, 9:334-340 Lories RJ, Derese I, Luyten FP: Modulation of bone morphogenetic protein signaling inhibits the onset and progression of ... supporting more active BMP signaling in the joint as CIA progresses Kinetics of bone morphogenetic protein ligands in collagen-induced arthritis – mRNA level Quantitative PCR for different BMP ligands ... differences were defined as P values of less than 0.05 Results Activation of bone morphogenetic protein signaling in collagen-induced arthritis Activation of BMP signaling during the course of CIA was...
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Báo cáo y học: " Management of subtrochanteric femur fractures with internal fixation and recombinant human bone morphogenetic protein-7 in a patient with osteopetrosis: a case report" pot
Ngày tải lên :
11/08/2014, 12:20
... treatment of nonunions in long bones, there were no data supporting its effectiveness in treating delayed long bone healing in patients with osteopetrosis He agreed to the use of rhBMP-7 and informed ... recommended the use of recombinant human bone morphogenetic protein- 7 (rhBMP-7) to his rightsided fracture, citing his history of delayed healing of fractures, as evidenced by the slow healing from radiographs ... Ragland PS, Biggins B, Friedlander G, Patel T, Cook S, Etienne G, Shimmin A, Kildey R, Rueger DC, Einhorn TA: Use of bone morphogenetic proteins for musculoskeletal applications An overview J Bone...
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Báo cáo y học: "Bone Morphogenetic Protein (BMP)-4 and BMP-7 regulate differentially Transforming Growth Factor (TGF)-β1 in normal human lung fibroblasts (NHLF)" pdf
Ngày tải lên :
12/08/2014, 11:22
... buffer (Invitrogen) following the manufacturer's instructions Protein concentration was determined using the BCA protein assay (Pierce), against a bovine serum albumin standard curve 15 μg protein ... described in Table Page of 11 Determination of total soluble collagen, tenascin C and fibronectin in cell supernatant The levels of total soluble collagen, tenascin C and fibronectin were assessed in ... also induces differentiation of fibroblasts into myofibroblasts and their subsequent proliferation, as well as collagen and other ECM protein production including tenascin-C (Tn-C) and fibronectin...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
Ngày tải lên :
19/02/2014, 00:20
... Dephosphorylation of the linker regions of Smad1 and Smad2 ⁄ by small C-terminal domain phosphatases has distinct outcomes for bone morphogenetic protein and transforming growth factor-beta pathways J ... activation of the p38 mitogen-activated protein kinase signaling pathway and induction of neurite outgrowth in PC12 cells by bone morphogenetic protein- 2 J Biol Chem 274, 26503–26510 Levine AJ & ... BMP signaling in stem cells tolerate lack of ALK3 in absence of the inhibitor Although functional proof is missing, the authors found up-regulation of ALK1 and ALK2 in these cells and suggest...
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Báo cáo khoa học: Type I receptor binding of bone morphogenetic protein 6 is dependent on N-glycosylation of the ligand pdf
Ngày tải lên :
23/03/2014, 07:20
... is inactive in these cells, probably due to its lack of binding to ActR-I (B) Signaling of BMP-6 and BMP-7 via ActR-I is shown in the inhibition of proliferation in the myeloma cell line INA6, ... determine binding affinities of the BMP ligand–receptor interaction Ligands were immobilized onto the surface of a CM5 sensor chip, and receptor ectodomain proteins were used as analyte Thus, interaction ... role in binding to the other type I receptors BMPR-IA and BMPR-IB This finding suggests that, in addition to the above-mentioned flexible binding epitope, usage of different main binding determinants...
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Báo cáo y học: "Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling" pot
Ngày tải lên :
09/08/2014, 10:21
... levels of bone remodelling seen in OA bone and perhaps a net gain of under-mineralized bone rather than the net loss of bone volume seen in OP Interestingly, and in contrast to the large number of ... breakdown of the cartilage and joint space narrowing, there is thickening and sclerosis of subchondral bone, development of cysts and bony overgrowth at the margins of the joint Despite an increase in ... by controlling both osteoblast and osteoclast differentiation and function [65-67] WNT signalling, in terms of bone mass and bone remodelling, is a very complex process that depends on the interplay...
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Báo cáo y học: "Suppression of bone morphogenetic protein inhibitors promotes osteogenic differentiation: therapeutic implications" doc
Ngày tải lên :
09/08/2014, 10:23
... range of questionable carriers is the current paradigm for bone induction in the clinical setting [9] The potential of inhibiting the inhibitors is therefore of great interest, because a single inhibitor ... with rh-noggin and transfected with bacillovirus-produced sclerostin They showed that combining noggin and sclerostin at a specific concentration resulted in a single complex, neutralizing their ... accelerated bone formation resulted from suppressing noggin expression in both in vitro and in vivo experiments After transfected osteoblasts (small-interfering RNA noggin) had been implanted into critically...
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Weekly intra-articular injections of bone morphogenetic protein-7 inhibits osteoarthritis progression pot
Ngày tải lên :
09/08/2014, 13:22
... Hammonds RG, Reddi AH: Natural bovine osteogenin and recombinant human bone morphogenetic protein- 2B are equipotent in the maintenance of proteoglycans in bovine articular cartilage explant cultures ... barely expressed in chondrocytes of the remaining cartilage in the control knees On the contrary, the rate of chondrocytes staining positive for BMP-7 was higher in the knees receiving treatment ... Pharmacokinetic analysis of BMP-7 in cartilage tissue (a) Outline for tissue the analysis Cartilage was obtained from knee joints at hour and 1, 2, 4, and days after intra-articular injection of 500...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
Ngày tải lên :
07/03/2014, 12:20
... value in Table 1) is taken as 100% (B) Basal incorporation of BrdU into proliferating LXF-289 cells in the presence of the inhibitors for the kinases calcium ⁄ calmodulin-dependent protein kinase ... kinase ⁄ (ERK1 ⁄ 2), p38 mitogen-activated protein kinase (MAPK), protein kinase C and PI3 kinase All values are means ± SD of 3–6 independent experiments run in triplicate (A) B 10 cisplatin, ... Activation of the protein kinase p38 in the spindle assembly checkpoint and mitotic arrest Science 280, 599–602 53 Beinke S & Ley SC (2004) Functions of NF-kappaB1 and NF-kappaB2 in immune cell...
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Báo cáo " A combination of the identification algorithm and the modal superposition method for feedback active control of incomplete measured systems " doc
Ngày tải lên :
28/03/2014, 13:20
... locations of the sensors The time delay is taken with 1/500 and 1/800 of total duration time T Some of the controlled results are shown in table and Fig and In Fig and 7, thin and dotted lines are uncontrolled ... be located to obtain a significant contribution of the information of xc This means a large norm of Cc in comparison with the norm of Cr Because the subinterval Tk ended, the information known ... system of infinite ordinary differential equations After that, the system of infinite equations is truncated to derive the state equation [11] In this calculation, the truncated system retains five...
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Báo cáo " A combination of the identification algorithm and the modal superposition method for feedback active control of incomplete measured systems" doc
Ngày tải lên :
28/03/2014, 13:20
... locations of the sensors The time delay is taken with 1/500 and 1/800 of total duration time T Some of the controlled results are shown in table and Fig and In Fig and 7, thin and dotted lines are uncontrolled ... be located to obtain a significant contribution of the information of xc This means a large norm of Cc in comparison with the norm of Cr Because the subinterval Tk ended, the information known ... system of infinite ordinary differential equations After that, the system of infinite equations is truncated to derive the state equation [11] In this calculation, the truncated system retains five...
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Báo cáo y học: " Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections" pdf
Ngày tải lên :
09/08/2014, 08:23
... severity of joint pathology Intra-articular injection of PGN into murine joints triggered arthritis in a dose-dependent manner [17] A single injection of this compound caused massive infiltration of ... binant human matrix metalloproteinase (MMP) protein expression in human fibroblasts by Staphylococcus aureus grown in the presence of recomPotentiation ofinterleukin (rhIL)-1β binant human interleukin ... μg/ml) and neomycin (50 μg/ml); and U930 was grown in the presence of tetracycline (5 μg/ml), 50 μg/ml kanamycin, and 50 μg/ml neomycin/ml for selection of the respective mutants Strains grown in...
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Báo cáo y học: "Early and late morbidity and mortality and life expectancy following thoracoscopic talc insufflation for control of malignant pleural effusions: a review of 400 cases" potx
Ngày tải lên :
10/08/2014, 09:22
... conceived of the study and participated in its design and coordination CA participated in the design of the study and performed the statistical analysis FP, GS, DP, AK, KL and IK took part in the ... occurred during hospitalization The cause of death was pneumonia in patients, pulmonary embolism in patients, myocardial infarction in patients and septic shock in patient The death rate within months ... duration of survival was inversely related to the extent of carcinomatous involvement of the pleura [13] These pleural fluid characteristics were not examined in our study The failure rate of videothoracoscopic...
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Báo cáo y học: " Decrease in expression of bone morphogenetic proteins 4 and 5 in synovial tissue of patients with osteoarthritis and rheumatoid arthritis" ppt
Ngày tải lên :
09/08/2014, 07:20
... for hyperplasia of the synovial lining layer, 1.9, 1.1 and 0.3 points for activation of the sublining stroma, and 2.1, 0.8 and 0.1 points for inflammatory infiltration in RA, OA and ND, respectively ... treatment of bone nonunions [7] In contrast, BMP signaling has been shown to be involved in the onset and progression of ankylosing enthesitis in spondyloarthropathies and in the induction of osteophytes ... understand precisely the network of morphogen action and regulation in the joint, because injection of BMP-2 induced osteophyte formation in a murine model [9] Thus, the interaction of BMPs and inhibitors...
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Báo cáo y học: "Bone morphogenetic proteins in destructive and remodeling arthritis" pdf
Ngày tải lên :
09/08/2014, 10:20
... causing joint ankylosis, thereby directly contributing to loss of joint function and disability In this concept, existing clinical boundaries are of less importance for the understanding of the ... FP: Bone morphogenetic protein signaling in joint homeostasis and disease Cytokine Growth Factor Rev 2005, 16:287-298 Lories RJU, Derese I, Ceuppens JL, Luyten FP: Bone morphogenetic proteins and ... signaling pathways including bone morphogenetic proteins (BMPs) may also be of importance in arthritis [4,8,9] BMPs and closely related growth and differentiation factors comprise a large group of...
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Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx
Ngày tải lên :
09/08/2014, 14:22
... 2) Staining was particularly prominent in the cytoplasm of the chondrocyte-like cells of the NP and IAF, with both single cells and those in clusters showing immunopositivity (Figures and 2) ... endothelial cells of Figure Examples of immunohistochemical staining for CDMPs in human intervertebral disc (row A) Cartilage derived morphogenetic protein (CDMP 1) and for CDMPs in human intervertebral ... [22] and bovine disc cells in alginate [21] have shown significant increases in cell proliferation Here we showed a small increase in proliferation of human disc cells in alginate culture following...
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Báo cáo y học: "Bone morphogenetic proteins, genetics and the pathophysiology of primary pulmonary hypertension" ppt
Ngày tải lên :
12/08/2014, 18:20
... Wilkins; 2000 Reddi AH: Interplay between bone morphogenetic proteins and cognate binding proteins in bone and cartilage development: noggin, chordin and DAN Arthritis Res 2001, 3:1–5 Wozney JM, ... signaling pathway [7,8], but also cell-type-dependent activation of other signaling pathways, including TGF-β activated kinase mediated p38 mitogen activated protein kinase [16,17] and protein kinase ... critical role in embryonic lung morphogenesis In contrast, little is known about the functional properties of these proteins in the adult Bone morphogenetic protein receptors Members of the TGF-β...
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