normal osteoarthritis and rheumatoid arthritis samples
Báo cáo y học: "Action of fibroblast growth factor-2 on the intervertebral disc" docx
... endogenous gene expression by cells from normal and and degenerative human intervertebral disc Fresh human nucleus pulposus tissue from normal (grades to 2) and degenerative (after surgery) intervertebral ... preparation, and statistical analysis HSA and FP participated in the study design, collection of human tissue samples, analysis and interpretation of the data ME participated in analysis and interpretation ... Alginate beads and monolayers were made for long-term and short-term analysis, respectively For alginate bead cultures, isolated NP cells and AF cells were resuspended in 1.2% alginate, and beads...
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Báo cáo Y học: The mechanism of nitrogen monoxide (NO)-mediated iron mobilization from cells NO intercepts iron before incorporation into ferritin and indirectly mobilizes iron from ferritin in a glutathione-dependent manner pot
... hydrazone (PIH) and its analogue, 2-hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone (311), were synthesized by standard techniques [34] Both PIH and 311 are strong Febinding ligands [34] and were ... concentrations of both physiological chelators (apolactoferrin and apoTf) and synthetic chelators (EDTA and DTPA) were used, and for all ligands no significant potentiation of 59Fe mobilization was ... overlying media and cells were then separated and the 59Fe levels in each assessed Cells were lysed and the cytosols subjected to native PAGE-59Feautoradiography (see Materials and methods) (B)...
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Báo cáo Y học: O-GalNAc incorporation into a cluster acceptor site of three consecutive threonines Distinct specificity of GalNAc-transferase isoforms pot
... vicinal effects, positive as well as negative, of GalNAc and Galb1–3GalNAc residues on the efficacy and pathway of incorporation of the second and the third GalNAc residues with four pp-GalNAc-Ts ... (Bio-Rad) with BSA as a standard The solutions were stored in aliquots at )80 °C until use Enzymatic GalNAc incorporation into peptide and glycopeptide acceptors The standard enzyme reaction mixture ... glycopeptides, and 0.2 U recombinant enzyme pp-GalNAc T1, T2, T3, or T4 (0.2268 lg, 1.098 lg, 1.365 lg, and 2.768 lg, respectively), in a final volume of 100 lL Reactions were performed at 37 °C for 16 h and...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... counter, ARVO; excitation and emission at 335 and 460 nm, respectively; Wallac, Turuk, Finland) The concentration of the oxidant in the samples was calculated using the standard curve, which was ... generation and oxidative stress with ageing and exercise: differential effects in the myocardium and liver Acta Physiol Scand 169, 343–351 Møller P & Loft S (2006) Dietary antioxidants and beneficial ... versus SOD On the other hand, excessive O2– production has a detrimental role in the pathogenesis of a number of disorders, including inflammation, rheumatoid arthritis, and asthma [44,45] Oxidative...
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Báo cáo khoa học: The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway doc
... antiserum 1311C as a diffuse band of 42–44 kDa (Fig 2, lanes 11 and 12) With antisera 1311N and 1311NC, we could not precipitate this product (Fig 2, lanes and 3, and 20 an 21), possibly because ... antisera (1311N, 1311NC and 1311C) immunoprecipitated this fragment in its deglycosylated forms, namely as proteins of 23 and 24 kDa (Fig 2, lanes 5, 14 and 23, and 6, 15 and 24, respectively) ... the 1311N and 1311C antisera detected a newly synthesized protein of 62 kDa and a less abundant protein of 64 kDa (Fig 2, lanes and 10) Both proteins represent intact forms of Ac45 and vary...
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Báo cáo y học: " Human immunodeficiency virus type 1 Vpr: oligomerization is an essential feature for its incorporation into virus particles" ppsx
... domains I, II and III dysregulate Vpr oligomerization and alter the ability of Vpr to incorporate into virus particles Page of 11 Materials and Methods Cells and plasmid HeLa, and 293T cells ... anti-Gag (p24) and anti-Vpr antibody Similarly, cells were pelleted and cell lysates were used to detect the presence and expression of Gag and Vpr in transfected culture Results Construction and characterization ... AS, JKS and VA conceived and designed the experiments, NJV, LAW, OT and YL performed the experiments, NJV, LAW, OT, AR, NY, AS, JKS, RCM and VA analyzed the data, NJV, LAW, OT, TL, TMD, AR and NY...
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Báo cáo y học: " The cellular source for APOBEC3G’s incorporation into HIV" pdf
... were lysed in hypotonic TE buffer, and the S1 fractions were either untreated (lane and 2) or treated with 0.5% Triton X-100 at 4°C (lane and 4) and 37°C (lane and 6), respectively Following the ... JM, XYL and JX have made equal contributions to conception and design, acquisition of data, and analysis and interpretation of data JM was involved in drafting the manuscript QZ, ZLL and PPJ ... Western blots of the P100 and S100 fractions were then probed with antibody specific for HA (top), TFR (middle), and caveolin-1 (bottom) S and P represent the S100 and P100 fractions, respectively...
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Báo cáo y học: "Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef" ppsx
... and mDC-IFNa were separately infected with WT NL(AD8) and ΔVpu, and the expression of tetherin and HIV-1 Gag in DCs at h and 3-7 days post-infection were assessed by immunoblotting The p24 bands ... [25] and tetherin (BST-2 or CD317) [26,27] Tetherin is a host transmembrane protein [26,27] and is expressed by a wide-range of human and animal cells [28,29] Mouse and human pDCs [30,31] and ... experiments and participated in the experimental design PS contributed to some experiment design and data analyses and provided tetherin anti-serum CMC and LW wrote the manuscript All authors read and...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... electrophoresis and measure of the absorbance at 260 and 280 nm Materials Semi-quantitative RT-PCR Ovine FSH (oFSH-21) was kindly provided by the National Institute of Arthritis, Metabolic and Digestive ... bFGF producing cells [2,33] and are the target of this growth factor as bFGF inhibits FSH-induced estradiol synthesis ([5], and our results) HSPG and especially glypicans and syndecans have been ... cell layer in M NaOH and subsequent neutralization by M KH2PO4, DNA was quanti®ed in a Kontron spectro¯uorimeter using Hoescht 33258 as ¯uorescent probe and calf thymus as standard 365 488 289...
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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
... Characteristics of CRLPs and pCRLPs The lipid content of the CRLPs and pCRLPs used is shown in Table The total cholesterol, triacylglycerol and phospholipid content, and the triacylglycerol/total ... antioxidants on atherosclerosis [49], and has been shown to bind to VLDL and LDL, and to provide the particles with greater antioxidant protection than vitamin E and many other antioxidants [50] The ... cholesterol) for 48 h, and the levels of mRNA for the genes indicated were determined by RT-PCR The bands were quantified by absorbance (OD) analysis and the values were normalized using those...
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Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins docx
... insoluble protein in the cases of GFP and hCypA and mostly soluble protein for HMP and PpiB Given that the chromophore in GFP involves a tyrosine (Tyr66), and that its photophysical properties ... pBH964) [18], PpiB (with and without an N-terminal His6 tag, using pND1098 and pKO1154, respectively), and cycle mutant GFP (using pMH1200) Plasmids pND1098 (His6-PpiB) and pKO1154 (PpiB) were ... either amino acid at 50 lm (data not shown) With 10 and lm DOPA, the yields were 20 and 50% lower, respectively, than with 10 lm tyrosine, and there was still discernible production of PpiB...
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Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
... degradation and removal of CS-chains from the complex resulted in the disappearance or a large reduction of the bands in the stacking gel and the band at Ó FEBS 2003 300 kDa, and new bands around ... reveal bands in the stacking gel and a band around 300 kDa (Fig 3A, lane 1) When the MMP-9/ CSPG complex was incubated with 10 mM CaCl2 for h at 37 °C prior to electrophoresis, both these bands ... both these bands either disappeared or were strongly reduced, and new bands with lower Mr appeared (Fig 3A, lane 2) Two weak bands at 80 and 85 kDa were seen along with a strong doublet at 74/76...
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Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
... collected 15 days after each injection and tested for affinity and specificity and stored at )20 °C Mouse monoclonal antibodies against Tyr-tubulin (Tub1A2) and total a-tubulin (DM1A), peroxidase-conjugated ... original volume with MEM buffer and kept cold (0 °C for 30 min) with gentle stirring The samples were then centrifuged at 100 000 g for 10 at °C, and the pellet and supernatant fractions were ... cytoskeletons, and fixed with anhydrous methanol at )20 °C The samples were washed, incubated with 2% (w/v) BSA in NaCl/Pi for 30 min, and stained by double indirect immunofluorescence using anti-nitro and...
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Báo cáo khoa học: Dissection of LolB function – lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers potx
... b-barrel, and two loops covering the barrel The barrel and the loops containing three a-helices form a hydrophobic cavity, which has recently been found to undergo opening and closing on binding and ... immunodetection system, as described in (B) The densities of the bands were determined and plotted as a function of the amounts of LolB and mLolB LolB and mLolB (each lg) were analysed by SDS–PAGE, followed ... coli phospholipids and 0.26 lgÆmL)1 mLolB for the indicated times The reaction mixtures were fractionated into pellet (p) and supernatant (s) fractions and analysed by SDS–PAGE and immunoblotting...
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Báo cáo khoa học: Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica) doc
... 7A, reruns of the purified quail (lanes and 4), chicken (lanes and 5) and guinea fowl (lanes and 6) ZP1 proteins showed a dominant band after western blotting and probing using antiZP1 serum (lanes ... light and 10 h darkness and were provided with tap water and a commercial diet (Tokai-Kigyo, Toyohashi, Japan) ad libitum The birds were killed by bleeding from the carotid artery, and the serum and ... (lane 2), chicken (lane 3), turkey (lane 4) and guinea fowl (lane 5) were subjected to SDS-PAGE and detected with CBB staining (C), or by ligand blotting and probing with radiolabeled ZP3 (D) Results...
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Báo cáo sinh học: " Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy" docx
... quantitative PCR after DNA isolation from the cell lysate, and the viral copy numbers were normalized to actin DNA in the samples The * indicates p < 0.05 and ** indicates p < 0.01 as analyzed by the Student's ... studies and in the preparation of the manuscript JLC assisted in the design of the study and manuscript preparation HW conceived of the study, participated in its design and coordination, and drafted ... receptor CAR, and delivered transgenes into both high-CAR and low-CAR cells more efficiently than the unmodified Ad5 vector Our data further showed Ad5.PTDtat infected cells via both CAR and PTDtat...
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Báo cáo sinh học: " The 3'''' sequences required for incorporation of an engineered ssRNA into the Reovirus genome" pot
... gene and protein σ2 to study the function of this protein and its role in reovirus replication and host cell interaction Additionally, it will now be possible to use reovirus as a vaccine and ... the Materials and Methods section We then examined the ssRNA, dsRNA, and CAT-dsRNA using Northerns, 12 hours following lipofection Thirty-six hours later, samples were harvested and plaque assays ... purification and/ or vaccine development Extension of these studies to larger genes should expand the effectiveness of this reovirus system Methods Virus and cell lines Reovirus ST3 strain Dearing and...
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Báo cáo hóa học: " Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy" ppt
... quantitative PCR after DNA isolation from the cell lysate, and the viral copy numbers were normalized to actin DNA in the samples The * indicates p < 0.05 and ** indicates p < 0.01 as analyzed by the Student's ... studies and in the preparation of the manuscript JLC assisted in the design of the study and manuscript preparation HW conceived of the study, participated in its design and coordination, and drafted ... receptor CAR, and delivered transgenes into both high-CAR and low-CAR cells more efficiently than the unmodified Ad5 vector Our data further showed Ad5.PTDtat infected cells via both CAR and PTDtat...
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Báo cáo y học: "CXCL12 is displayed by rheumatoid endothelial cells through its basic amino-terminal motif on heparan sulfate proteoglycans" ppsx
... labelling of CXCL12/HSPGs (OA) synovial tissues Double arthritis (RA) and osteoarthritisand CXCL12/MECA-79 of rheumatoid arthritis (RA) and osteoarthritis (OA) synovial tissues Frozen RA or OA sections ... cytometry and immunofluorescence studies, FB and FAS prepared the peptides and antibodies used and contributed to drafting and reviewing the manuscript, GP and IGC contributed to the collection and ... Binding (RA) ECs and cytokine-treated HUVECs and rheumatoid arthritis (RA) ECs and cytokine-treated HUVECs Endothelial cells (ECs) were incubated with 300 nM biotinylated CXCL12α and, after extensive...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose