nogo mag and omgp and their impact on axon regeneration
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... selection, cells were cloned by limiting dilution and the clones analysed by FACS for coexpression of CCR5 and CD4 Western blot analysis and detection 35 S-labelled CCR5-myc-his CCR5-myc-his containing ... consequence of the lipid concentration, because the detergent partitions between the solution, protein and membrane, but only the concentration in the membrane determines the outcome of reconstitution ... Efficient reconstitution is strongly dependent on detergent concentration for a given lipid/protein ratio [44] The optimal detergent concentration was frequently found to be close to that at the onset...
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Báo cáo khoa học: Involvement of NF-jB subunit p65 and retinoic acid receptors, RARa and RXRa, in transcriptional regulation of the human GnRH II gene pot
... estrogen-responsive element site and an SP1 site at positions )1252 ⁄ )1256 and )1726 ⁄ )1717, respectively [3] In addition to cAMP and estrogen, other hormonal regulation of GnRH II expression has ... effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the promoter ... negative controls (No immunoprecipitation, lane 3; anti-rabbit IgG, lane 7; and PCR negative, lane 8) These controls indicate that there was neither nonspecific precipitation nor PCR contamination Positive...
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Báo cáo khoa học: ABCG transporters and disease pdf
... Harrison AA, Highton J, Jones PB, Stamp LK et al (2010) A strong role for the ABCG2 gene in susceptibility to gout in New Zealand Pacific Island and Caucasian, but not Maori, case and control ... studies will continue to uncover additional novel physiological substrates and functions for ABC transporters and define additional roles in human disease The powerful combination of genetic and physiological ... transfer by ABCG5 and ABCG8: in vitro assay and reconstitution J Biol Chem 281, 27894–27904 18 Wang J, Zhang DW, Lei Y, Xu F, Cohen JC, Hobbs HH & Xie XS (2008) Purification and reconstitution of sterol...
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báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
... cell invasion and migration because it contains thrombospondin-type repeats [130] Step 7- Cell Adhesion, Differentiation & Migration: Focal Adhesion Kinase & Receptors Focal Adhesion Tyrosine ... processes involved in cell-adhesion and protein-protein interactions necessary for enhanced virus replication, cell growth, migration and invasion Expression of BAI1 and BAI3 receptors in HIV-infected ... diverse cellular functions, including cell-cell interactions, cell adhesion, cell aggregation, cell migration, cell cycle progression, differentiation, inflammation, angiogenesis, and maintenance of...
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báo cáo khoa học: " A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptors ETR1 and ERS1" ppsx
... hypocotyl elongation in comparison with the wild-type WS control at all tested ethylene concentrations, which is consistent with dose-response analysis of the etr1-7 loss-of-function mutant (Fig ... no mutation being isolated for ERS1 [13] Loss-of-function (LOF) mutations in any single ethylene receptor demonstrated little or no effect upon seedling growth, consistent with functional overlap ... ETR1 and ERS1 play more predominant roles in the regulation of ethylene responses in Arabidopsis than previously suspected Results Isolation of the ers1-3 and etr1-9 T-DNA insertion mutations...
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Báo cáo khoa học: " Monocarboxylate Transporters and Lactate Metabolism in Equine Athletes: A Review" pdf
... membranes prevents the passage of protons and lactate anions (Juel 1997) The role of nonionic diffusion increases with increasing lactic acid concentration and also when pH decreases It can be ... tarandus) Comp Biochem Physiol 1996, 114A, 277-281 Räsänen LA, Lampinen KJ, Pösö AR: Responses of blood and plasma lactate and plasma purine concentrations to maximal exercise and their relation ... respiration Protein content within the cell is high, and proteins function as buffers, as does the dipeptide carnosine The concentration of the latter is especially high in equine muscles and is concentrated...
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Báo cáo y học: " Expression of eicosanoid receptors subtypes and eosinophilic inflammation: implication on chronic rhinosinusitis" pdf
... expressed on Th2 cells (CRTH2) [12] In airways, PGE2 may induce bronchodilation and airway relaxation by acting via EP2 receptor [13,14] Basal expression of EP2 and EP4 receptors is increased on bronchial ... modulation of inflammation and immune responses by prostaglandins and thromboxanes J Clin Invest 2001, 108:15-23 Peacock CD, Misso NL, Watkins DN, Thompson PJ: PGE2 and dibutyryl cyclic adenosine monophosphate ... differentiation, recruitment and maturation [22-24] In other hand, we did not found any changes in BLT receptors expression between controls and disease groups These findings correspond with previous...
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Báo cáo khoa học: "Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland" ppt
... minor (50%) Results Hormone concentrations and ovary evaluation (intact mares) The results of the oestradiol and progesterone concentrations and ovarian findings are summarised in ... collection and fixation Adrenal glands were collected immediately after euthanasia in all mares and weighed However, time from collection to fixation of the tissue was from 20 minutes up to one hour, ... manufacturer's instructions The relative crossreactions of the antibody were 0.9% with corticosterone and 0.1% with testosterone The inter- and intra-assay coefficients of variation for progesterone were as...
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Báo cáo y học: "Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeas" potx
... Distribution among different sensitivity/resistance groups and functional classification of metal tolerance affecting mutations Distribution among different sensitivity/resistance groups and functional ... iron supplentatio on cadmium and nickel tolerance Effect of4 Effect of iron supplementation on cadmium and nickel tolerance Serial dilution assays comparing the iron uptake impaired deletion ... responsive [11] Their identification among metal sensitivity-conferring mutations suggests that not only the clearance of damaged (or unwanted) proteins by the proteasome and transcriptional...
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The role of adenosine, adenosine receptors and transporters in the modulation of cell death
... including the hyperpolarization and depolarization of mitochondria, translocation of Bax and cytochrome c, elevation of cytosolic Ca2+ level, cellular acidification and activation of caspases An intrinsic ... including the hyperpolarization and depolarization of mitochondria, translocation of Bax and cytochrome c, elevation of cytosolic Ca2+, cellular acidification and activation of caspases An intrinsic ... Pharmaceutical Implications 218 vii Chapter Conclusion and Future Directions 5.1 Conclusion 219 5.2 Future Directions 221 5.2.1 Studies of Adenosine-Induced Apoptosis 221 5.2.2 Adenosine and Adenosine...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... different conformations when immobilized on the biosensor chip The observation of two kinetic components in the association and dissociation kinetics also suggests the presence of two populations of ... internalize and degrade [125I]TC-PCSK9 at 37 °C However, the interaction of ligands with cell surface receptors at 37 °C is a function not only of the rate constants of association and dissociation, ... prediction was tested by comparing the dissociation rate for binding under the pre-equilibrium conditions (60 min) and equilibrium conditions (240 min) As can be seen in Fig 5A, dissociation of...
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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression profiling ppt
... of AaE75 and AaHR3 expression, we propose that these NRs could compete for their common response element on the AaVg promoter acting in the regulation of AaVg synthesis and termination The NR ... of fluctuation in the protein levels of AaEcR and AaUSP isoforms during vitellogenesis [13], suggest a lack of significant transcriptional and translational regulation of the two components 1242 ... protein–protein interactions AaHR38 and AaSvp, during the arrest and termination of vitellogenesis, respectively, bind to AaUSP preventing its heterodimerization with AaEcR, and, consequently, the 20Edependent...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... between the b3-strand and the a3-helix; (b) the catalytic machinery consists of the b4-strand aspartate (nucleophile), b5-strand glutamate (proton donor) and b7-strand aspartate (transition-state stabilizer); ... indicating evolutionary relationships of all these enzymes and proteins and hinting at their eventual evolutionary histories It is worth mentioning that to understand the common evolutionary history ... region in rBAT and 4F2hc proteins, which should appear more parsimoniously only once; and (b) the gain of the analogous function Because the family GH13 enzymes are spread throughout the whole taxonomy...
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Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
... production and extracellular acidification), we found no evidence for a functional response, even at high ligand concentrations (exceeding micromolar) using pNPY, pPYY, cPYY and cPP (only cPYY ... polymerase, on an ABI PRISM 310 Genetic Analyzer, and found to be identical to the genomic sequence The expression construct contains one upstream in-frame methionine codon (immediately after the cloning ... Experimental procedures Isolation and sequencing of the chicken Y6 gene and cloning into an expression vector Degenerate PCR primers, based on several mammalian and the nontetrapod Y1 subfamily, were...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... oligomerization [1,2] In the present study we used two agonists (dopamine and NPA) and one inverse agonist (raclopride), and demonstrated that none of these ligands affects the oligomerization process ... fusion protein [47] In conclusion, we have demonstrated that the dopamine D2L and D2S receptors can form constitutive homo- and hetero-oligomers in two expression systems (Sf9 and HEK293 cells) and ... National Institute for Biological Standards and Controls (NIBSC, London, UK) [3H]Spiperone was from Amersham International (Bucks., UK) All the other reagents were obtained as indicated Construction...
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Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx
... elution conditions Disulfide bond formation and protein purification The reduced synthetic peptide was oxidized in a refolding buffer (0.1 M sodium acetate, M GdnHCl and mM EDTA, pH 7.8) containing ... Addition of lM sWntx-5 to the standard solution did not block neuromuscular transmission, even after 30-min incubation In contrast, the potent a-cobratoxin used as a control, on both washed out and ... for WTX from N kaouthia Considering that the two toxins display 11 residue differences and that the competition systems used (human and rat on one hand, and chicken on the other) are not identical...
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Tài liệu Báo cáo Y học: Human and Drosophila UDP-galactose transporters transport UDP-N-acetylgalactosamine in addition to UDP-galactose doc
... suppressed by one of the additional mutations introduced into the hUGT1(N5aaCST)-cHA mutant To identify the second mutation responsible for the suppression of the N47A phenotype, we ®rst constructed ... examined the expression levels of these mutant proteins and their intracellular distribution As shown in Fig 9, introduction of the I51T mutation suppressed the N47A mutation and resulted in the ... using a Kodak IS440CF image analysis system (NEN Life Sciences) DNA sequencing Subcellular fractionation and nucleotide sugar transport assay The subcellular fractionation and transport assay were...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... reduction and alkylation produced only one band in SDS/PAGE with the expected molecular mass of the monomer Considering that dimerization could occlude the TCR binding site, we also constructed ... NaCl/Pi and concentrated to mgÆmL)1 hVb2.1hCb2 and hVb1hCb2 were also produced as inclusion bodies and refolded at pH 8.5 Purification steps included gel filtration on a Superdex 200 FPLC column and ... [26] On the contrary, immobilization of monomer wtSSA and SSA(C26S) yielded a Kd ¼ 10 lM with soluble Vb5.2, thus confirming the dissociation constant value for the couple SAg–TCR In addition,...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... B1CB2 and B1YB2 (Fig 2B) Agonist-induced internalization of modified B1KB2 constructs Phosphorylation patterns of B2wt and of B1 ⁄ B2 chimeras reflect their agonist-inducible internalization The ... phosphorylation The chimera on the other hand displayed little basal phosphorylation in the absence of their agonist DAK, although this may, in part, be a sensitivity problem due to their lower expression ... with 250 lgÆmL)1 hygromycin B [3H]Agonist binding studies For the determination of dissociation constant Kd and receptor number Bmax, confluent monolayers on 24-well plates (B2wt) ⁄ 12-well plates...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... experimental conditions for the production of the I7 OR in yeast and used biochemical and immunological methods to estimate the levels of receptor expression and its cellular localization Results ... band corresponds to the receptor monomer The 51 kDa band may correspond to a glycosylated form of the receptor since I7 OR contains two N-glycosylation sites, one at the N terminus and another ... odorant and ⁄ or its solvent toxicity to yeast cells or to receptor desensitization at the highest ligand concentrations It was checked that a final odorant concentration of · 10)5 m corresponds...
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