... thống ubiquitin- kinh gây b i rối loạn ch m ối v i bệnh Parkinson, có nhi u nghi ngờ v vai trò protein UCH-L1 (Ubiquitin Carboxyl -terminal Hydrolase L1), protein có vai trò trung tâm hệ thống ubiquitin- proteasome, ... cuối C Glycine ubiquitin lysine củ phân tử ubiquitin khác g n vào phân tử , tiếp tụ trình phụ thuộ y hình thành chu i ubiquitin Quá trình ubiquitin hóa c thực b i ba enzyme: E1 - ubiquitin activating ... thông qua thioester E2 - ubiquitin conjugating enzyme - m Trang ạt hóa ổ tốt nghiệp ạt E3 - ubiquitin ligase - chuy ủ ả ệ ến g m m ệ ễ Kh u nhóm terminal carboxyl ubiquitin c g n vào c u nối...
... regression of soluble E-selectin and anti-Sip1 Cter IgM.l Correlation and linear regression of soluble E-Selectin (ng/ml) and serum IgM specific to the carboxy- terminal subunit of Sip1 (optical ... clone, is 427 residues long and has 100% identity with the carboxy- terminal subunit of the splicing factor Sip1 (Figure 1a) The expected molecular size of 48 kDa, the purity and the immunoreactivity ... antigens, we identified a strongly reactive clone encoding the carboxy- terminal subunit of the splicing factor Sip1 (Sip1 C-ter) The carboxy- terminal region of Sip1 – a novel endothelial autoantigen...
... Sequence similarity between NS4B carboxyterminal domain and the membrane binding domain of D-lactate dehydrogenase Sequence similarity between NS4B carboxyterminal domain and the membrane binding domain ... and domain distribution mutants4 D-lactate NS4B carboxy membrane binding Cellular in Huh7 cellsof dehydrogenase terminal domain Cellular distribution of NS4B carboxyterminal domain mutants and ... first and second panels respectively Third panels show merged images by characteristics in the domain other than the cysteines at positions 256 and 260 Membrane association of the carboxy terminal...
... Sequence similarity between NS4B carboxyterminal domain and the membrane binding domain of D-lactate dehydrogenase Sequence similarity between NS4B carboxyterminal domain and the membrane binding domain ... and domain distribution mutants4 D-lactate NS4B carboxy membrane binding Cellular in Huh7 cellsof dehydrogenase terminal domain Cellular distribution of NS4B carboxyterminal domain mutants and ... first and second panels respectively Third panels show merged images by characteristics in the domain other than the cysteines at positions 256 and 260 Membrane association of the carboxy terminal...
... by caspase-3 and/ or -6 (double amino acid-substitution mutants p65 D94E;D97E-tag (lanes and 4) and p65 V91L;D94E-tag (lanes and 10) were resistant to cleavage by both caspase-3 and caspase-6; ... produced in T cells (PBLs and Jurkat) that did not undergo apoptosis On the contrary, they showed a high viability and an increased NF-κB-dependent activation This carboxy- terminal fragment of p65/RelA ... LMB or PMA for and hours respectively Ten micrograms of cytosolic and nuclear protein extracts were analyzed by immunoblotting (IB) using specific antibodies against IκBα and the carboxy- terminus...
... contribution of Met1, and Met23 substituted for Leu, Fig Comparison of the cyclization reaction as a function of temperature and pH (A), (B) and (C): (d), (s) and (.) correspond to pH 2.5, 8.0 and 11.5, ... The pH was adjusted to 2.5, 8.0 and 11.5 and the samples were incubated at 37 °C, 50 °C and 70 °C Aliquots were taken at different time-points from each sample and analyzed by MALDI-TOF MS The ... hydrolysis reaction as a function of denaturing agent and pH (A), (B) and (C): (d), (s) and (.) correspond to guanidinium chloride concentrations of , and M, respectively Hydrolysis was calculated as...
... also called TIF2), and activator for thyroid hormone and retinoid receptors (ACTR) (also called RAC3, pCIP, AIB1 and TRAM1) These co-activators bind directly to the DNA-bound NRs and apparently function ... [19] Furthermore, CBP and CARM1 also Autoregulation of GRIP1 functions via C -terminal region support synergistic cross-talk through their HAT and HMT specificities for histones and other transcriptional ... interact with this C -terminal region in vitro GST–GRIP1-1305–1398 pulled down full-length GRIP1 (amino acids 5–1462) and C -terminal GRIP1 fragments (amino acids 1122–1462) but not N -terminal (amino...
... 22.2% α-helix, 23.3% β strand, and 37.2% coil in His-CI Native CI also shows nearly identical CD spectrum at 30°C and was found to carry ~29.9% α-helix, 20% β strand, and 34.2% coil (data not ... binding and kinetic study Equilibrium binding and kinetic study Equilibrium binding of CI to O64 (A) and OL (B) operators was studied according to standard method as described in Materials and methods ... performed with chymotrypsin and trypsin separately according to standard techniques As shown in Fig 1A, two major protein fragments of nearly 16 and 10.5 kDa (designated c and e, respectively) were...
... 22.2% α-helix, 23.3% β strand, and 37.2% coil in His-CI Native CI also shows nearly identical CD spectrum at 30°C and was found to carry ~29.9% α-helix, 20% β strand, and 34.2% coil (data not ... binding and kinetic study Equilibrium binding and kinetic study Equilibrium binding of CI to O64 (A) and OL (B) operators was studied according to standard method as described in Materials and methods ... performed with chymotrypsin and trypsin separately according to standard techniques As shown in Fig 1A, two major protein fragments of nearly 16 and 10.5 kDa (designated c and e, respectively) were...
... only after 10 and 20 h of infection, and IL-7 level was elevated only Figure epithelial cells S pneumoniae induce the release of distinct CC and CXC, as well as Th1 and Th2 cytokines and growth ... experimental design and drafted the manuscript KM participated in the study design and performed biochemical and cellular studies JZ participated in the study design and performed biochemical and cellular ... the study design and performed molecular studies PG participated in the study design and performed molecular studies BO participated in the study design and performed biochemical and cellular studies...
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... mutant ubiquitin inhibits the interaction between the vi hydrophobic surface of ubiquitinand the ubiquitin- binding protein p62/SQSTM1, showing conceptually that Cif activity impairs ubiquitin/ ubiquitin-like ... two discrete phases requiring an ensemble of players: ubiquitin conjugation (ubiquitination) and degradation 1.2 Ubiquitination Ubiquitinand UBLs typically modulate protein function following ... (ubiquitin activating enzyme), E2 (ubiquitin conjugating enzyme) and E3 (ubiquitin ligating enzyme) (Hershko and Ciechanover 1998) In an ATP dependent manner, an E1 initially adenylates the carboxyl-terminal...
... of Baxand Bak on mitochondrial membranes, which liberates cytochrome c and activates caspase and the apoptosome [23] Transfection of tBid directly triggers mitochondrial-dependent apoptosis and ... general mechanism of control of apoptosis by ubiquitin ligases Materials and methods Plasmids, antibodies and reagents All plasmids encoding Itch and Myc -ubiquitin have been described previously ... gift of the Bid vectors, and P S McPherson and P A Barker for useful discussion and advice We are also extremely grateful to Michel Bouvier and Billy Breton for guidance and assistance in our BRET...
... of polyubiquitin (A) Interaction of NUB1 with polyubiquitin linked by a-peptide bonds GST and GST-fused ubiquitin monomer and polyubiquitins (a-peptide linkage) were expressed in bacteria and purified ... thiol-proteases that include ubiquitin C -terminal hydrolases (UCHs) andubiquitin isopeptidases UCHs cleave the linear ubiquitin precursor linked by a-peptide bonds, such as UbC1, while ubiquitin isopeptidases ... trimer), and GST-UbC1 (ubiquitin nanomer) The ubiquitin trimer and nanomer were polyubiquitin linked by a-peptide bonds Treatment with a thiol-blocking reagent To inhibit the activities of C-terminal...
... [6-8] In this system, the ubiquitin is split into N -terminal ubiquitin (Nub) and C -terminal ubiquitin (Cub) but the Nub and Cub have high affinity towards each other and is able to reassemble ... back together spontaneously into ubiquitin protein When Nub and Cub moieties bind together within a cell, this would activate the ubiquitin- specific protease (UBP) and hence, the transcriptional ... plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting proteins 115 Results and Discussion 3.1 Principle of Split-Ubiquitin...
... lập số phải kể đến promoter ubiquitin gen ngô, promoter gen rbcS (ribulose – 1,5-bisphosphate carboxylase small subunit), promoter gen mã hóa sucrose synthase [4] Ubiquitin - protein sốc nhiệt ... chuyển gen Do gen Ubiquitin biểu hầu hết mô thực vật điều kiện tự nhiên, nên promoter ubiquitin sử dụng để biểu gen loại thực vật chuyển gen đặc biệt loài gần gũi Sự biểu mạnh promoter ubiquitin chi ... điều khiển Ubiquitin thực vật 14 2.1 Lemna aequinoctialis DB1; Spirodela polyrrhiza DB2 21 3.1 Điện di DNA tổng số gel agarose 0,8% 33 3.2 Sơ đồ cấu trúc đoạn yếu tố điều khiển biểu gen ubiquitin...
... đương với BNP chẩn đoán suy tim ABSTRACT ROLES OF NT-PROBNP (N -TERMINAL B-TYPE NATRIURETIC PEPTIDE) IN DIAGNOSIS OF HEART FAILURE Vu Hoang Vu, Dang Van Phuoc * ... department, Cho Ray hospital from May, 2005 to March, 2006 including 152 heart failure pateints and 98 non-heart failure patients Results: NT-proBNP level in patients with heart failure were higher ... 89%, sensitivity of 90%, specificity of 89% There was no differ significant beetween NT-proBNP and BNP in diagnosis of heart failure (AUC of NT-proBNP: 0,981, AUC of BNP: 0.963; 95% CI = 0.004-0.041;...