... Hosokawa H, Sawamura T, Kobayashi S, et al Cloning and characterization of a brain-specific amino acid transporter J Biol Chem 1997; 272: 8717-8722 50 Bommarius AS, Makryaleas K, Drauz K An enzymatic ... was earlier reported (20) Apart from NO synthesis L-arginine may also serve asa substrate for glutamate formation and may also provide increased substrate for arginase (50) No significant changes ... et al (28) as described by Sadasivudu et al (29) Estimations of NO, TBARS and TAS: NO was estimated as NOx by Griess reaction after conversion of nitrate to nitrite by nitrate reductase, as described...
... from each hourly interval were thawed, and 20 μl of each sample were analyzed NO was measured as NO2 - /NO3 - by the nitrate reductase method [29] using a commercially available kit (Cayman Chemical, ... latent TGF-β1 (panel B) and active TGFβ1 (panel A) was maintained across all groups The substantial levels of latent TGF-β1 in female rats at baseline and after CYP was accompanied by only minor ... levels of active and latent/total TGF-β1 at baseline and after CYP Urine levels of active and latent/total TGF-β1 at baseline and after CYP Active and latent/total TGF-β1 values are reported as pg/mg...
... nitricoxide synthase after sensitization and allergen challenge of Brown Norway rat lung Br J Pharmacol 1997, 121:1241-1246 27 Prado CM, Leicki-Maldonado EA, Arata V, Kasahara DI, Martins MA, ... 12 Barnes PJ: NO or noNO in asthma? Thorax 1996, 51:218-220 13 Kharitonov SA, Yates D, Robbins RA, Logan SR, Shinebourne EA, Barnes PJ: Increased nitricoxide in exhaled air of asthmatic patients ... analyzer was calibrated with Bronchoalveolar lavage (BAL) BAL was performed immediately after ENO sampling The lungs were lavaged twice through the tracheal cannula with 10 mL of saline (total:...
... 381S Badamchian, M., Spangelo, B.L., Bao, Y., Hagiwara, Y., Hagiwara, H., Ueyama, H & Goldstein, A. L (1994) Isolation of a vitamin E analog from a green barley leaf extract that stimulates release ... participate in the TS effect, and that the inhibitory effect of a- T was mediated by a nonantioxidative reaction a- T was reported to decrease PKCa activity due to activation of protein phosphatase 2A ... highest grade commercially available Data were expressed as the mean ± standard deviation of at least three independent experiments Statistical significance was assessed by multiple-comparison test...
... only an a- amino moiety (NOHAgma) interacted with iNOSoxy with an affinity (Kd 250 lm) much lower than that found for BuNOHG, and the NOHA analogue bearing only an a- carboxylate moiety (NOHGPA) ... N-aryl-N¢-hydroxyguanidines that lead to kcat values of formation of NOas high as 83 and 69% that obtained for NOHA (Table 2) As in the case of the N-hydroxyguanidines, the kcat values of NO formation from ... experiments are shown in Table NOHA was found to bind to iNOS with a Kapp value slightly lower than that of l-Arg (18 ± lm, Table 1) Homo-L-Arg and homo-NOHA, the l-Arg and NOHA analogues bearing one...
... regulatory factors Clin Diagn Lab Immunol 9, 530–543 26 Funatogawa, K., Matsuura, M., Nakano, M., Kiso, M & Hasegawa, A (1998) Relationship of structure and biological activity of monosaccharide ... H2SO4 and the absorbance was measured at 450 nm Quantification of each cytokine (in ngÆmL)1 for IFN-c and in pgÆmL)1 for IL-12p70) was performed based on the standard curve in each assay Preparation ... from the American Type Culture Collection (Manassas, VA, USA) and maintained in our laboratory, was also used For cell culture, RPMI-1640 (Dainippon Pharmaceutical Co Ltd, Osaka, Japan) supplemented...
... immunoblotting analysis was performed as described above The immunoreactive material was detected and quantified as described above Assay of NOS activity NOS activity was assayed by detecting the ... The Authors Journal compilation ª 2008 FEBS 2503 In vivo degradation of NOS and HSP90 by calpain M Averna et al Table Levels of native and 15 kDa calpastatin species in brain and aorta of NMS and ... aorta, the inhibitor was predominantly inactivated As both the inactivation and fragmentation of calpastatin are known to be produced by active calpain [32], these observations further indicate...
... sensory nucleus and the lateral parabrachial nucleus of the brainstem A small number of scattered NPS-positive cells were found in other brain areas, such as amygdala and hypothalamus The NPS-producing ... produce a transient increase in intracellular free Ca2+, indicating that NPS might be an excitatory transmitter in vivo by elevating intracellular Ca2+ A radiolabled analog of NPS (125I-labeled ... profound increase in locomotion, measured as the total distance traveled over one hour It is well known that animals naturally show increased exploratory activity when they are exposed to a novel environment...
... NGF-treated cells showed a significant increase in AP-1 and CRE activity at 12 hr, while NF-κB activity was not affected Asa result, there was also a significant increase in AP-1 and CRE activity ... rat iNOS were as follows; forward 5'-CAC GGA GAA CAG AGT TGG-3' and reverse 5'-GGA ACA CAG TAA TGG CCG ACC-3' Amplified samples were run on agarose gels and stained with ethidium bromide Images ... interleukin-1beta; iNOS, inducible nitricoxide synthase; MAPK, mitogen activated protein kinase; NF-κB, nuclear factor kappa B; NGF, nerve growth factor; NO, nitric oxide; nNOS, neuronal nitricoxide synthase;...
... Neuropharmacological data indicate that Abeta toxicity is mediated by an excitotoxic cascade involving blockade of astroglial glutamate uptake, sustained activation of NMDA receptors 2+ and an overt ... spatial and temporal regulation of cortical perfusion following basal forebrain activation Aluminium accumulation in the brain can alter neuronal signal transduction pathways associated with glutamate ... and contralateral) was measured by the Lowry method using bovine serum albumin (Sigma, USA) asa standard [18] Statistical analysis Results are given as means ± SD Experimental group comparisons...
... up-regulation of nitricoxide and tumor necrosis factor-alpha from peritoneal macrophages J Ethnopharmacol 2004, 95, 69-76 Kim YM, Bombeck CA, Billiar TR Nitricoxideasa bifunctional regulator of apoptosis ... conducted to examine the potential use of the novel β-glucan purified from P polymyxa JB115 as an immunostimulant or as an adjuvant of some animal vaccines β-glucan-induced nitricoxide production ... YJ Macrophage activation by polysaccharide fraction isolated from Salicornia herbacea J Ethnopharmacol 2006, 103, 372-378 12 Li J, Li DF, Xing JJ, Cheng ZB, Lai CH Effects of beta-glucan extracted...
... that of the glycerinated saline Data analysis An intention-to-treat approach was followed in the analysis of efficacy data All patients with a baseline and at least one postrandomization measurement ... Prieto has served as consultant to GSK, Novartis and Stallergenes, and has received grant founding from GSK and Novartis; R Palacios and D Aldana are employees of Diater Laboratorios SA; A Ferrer, ... moderate or serious severity Specific immunotherapy Alternaria alternata extract and nAlt a1 were produced by Diater (Madrid, Spain) Raw material containing spores and mycelia of Alternaria Alternata...
... 2-way ANOVA with repeated measures was used, as appropriate, to assess group means This was followed by the Scheffé multiple-range test for post hoc assessment of individual means P < 0.05 was ... oxygenase: contrasts to nitricoxide synthase Proc Natl Acad Sci USA 2001, 98:10996-11002 42 Kaur C, Sivakumar V, Ang LS, Sundaresan A: Hypoxic damage to the periventricular white matter in neonatal ... therapeutic applications Physiol Rev 2006, 86:583-650 37 Satoh T, Baba M, Nakatsuka D, Ishikawa Y, Aburatani H, Furuta K, Ishikawa T, Hatanaka H, Suzuki M, Watanabe Y: Role of heme oxygenase-1 protein...
... pentobarbital sodium (50 mg/kg) and placed on a heating pad The trachea was intubated to facilitate ventilation, and the femoral artery was cannulated for monitoring systemic arterial pressure (SAP) ... proteins: RAMPing up adrenomedullin signaling Mol Endocrinol 2007, 21:783-796 Kuwasako K, Kitamura K, Ito K, Uemura T, Yanagita Y, Kato J, Sakata T, Eto T: The seven amino acids of human RAMP2 and RAMP3 ... light and electron microscopy Brain Res 2000, 853:245-268 Takahashi H, Watanabe TX, Nishimura M, Nakanishi T, Sakamoto M, Yoshimura M, Komiyama Y, Masuda M, Murakami T: Centrally induced vasopressor...
... obstruction was classified as asthma when FEV1was 12% as compared to baseline and at least 200 ml and lung volumes returned to predicted normal range An incomplete bronchodilator response was stated ... 12% and > 200ml but norm value not reached Δ FEV1 > 12% and > 200ml and norm value reached Overlap between asthma and COPD Asthma Yes NoNo OAD Figure tive airway disease) Diagnostic decision making ... small risk of inducing severe bronchospasm [7] A promising non-invasive and easily available method for diagnosing asthma seems to be the measurement of fractional exhaled nitricoxide (FENO),...
... moderate to severe BHR positive bronchodilator reversibility (n = 58)§ *prevalence of asthma = 46.9%, prevalence of 'no asthma' = 53.1% § prevalence of asthma = 36,3%, prevalence of 'no asthma' ... Schermer T, Gindner L, Laux G, Szecsenyi J, Meyer FJ: Diagnosing asthma in general practice with portable exhaled nitricoxide measurement – results of a prospective diagnostic study Respir Res ... Nair A, Lipworth BJ: Portable exhaled nitricoxide measurement: Comparison with the "gold standard" technique Chest 2007, 131:410-414 Publish with Bio Med Central and every scientist can read...
... discontinuation of NO 150 ppm, hemodynamic and respiratory paramaters were measured at the same ventilator settings as in phase Statistical analysis Cardiorespiratory parameters at control were compared between ... fracture Measurements Systolic and diastolic arterial pressures (SAP and DAP), and systolic and diastolic pulmonary arterial pressures (SPAP and DPAP) were simultaneously measured using the arterial ... DPAP, pulmonary capillary wedge pressure (PWP), right atrial pressure (RAP), VT, Paw and gas flow were recorded at a paper speed of 50 mm/s Mean arterial pressure (MAP) was calculated as 1/3 SAP...
... JA, Hamada K, Mehta S, Scott JA, Jiao A, Yandava CN, Kobzik L, Wolyniec WW, Fabian AJ, Venugopal CS, Grasemann H, Huang PL, Drazen JM: Contribution of nitricoxide synthases 1, 2, and to airway ... in association with human airway inflammatory diseases such as asthma, does not seem to occur in mouse models of asthma [43] Therefore the importance of granule proteins, such as heme peroxidases, ... [abstract] Am J Respir Crit Care Med 2000, 161 :A5 42 44 Honda K, Kobayashi H, Hataishi R, Hirano S, Fukuyama N, Nakazawa H, Tomita T: Inhaled nitricoxide reduces tyrosine nitration after lipopolysaccharide...
... Critical Care Vol 11 No Salem and Mebazaa no difference in secondary outcomes such as resolution or duration of the CS, New York Heart Association functional class and 6-month mortality There was, ... CS with early acute MI primary angioplasty remains the gold standard Percutaneous left-ventricle-assisting devices may serve asa bridge to recovery or final treatment, namely transplantation Competing ... patients with cardiogenic shock: looking for the reasons JAMA 2007, 297:1711-1713 Dzavik V, Cotter G, Reynolds HR, Alexander JH, Ramanathan K, Stebbins AL, Hathaway D, Farkouh ME, Ohman EM, Baran...
... Medical School, an Associated Physician at Massachusetts General Hospital, and an employee of Novartis Pharmaceuticals Novartis Pharmaceuticals was otherwise not involved in this research and ... tilation-induced activation of apoptosis signal-regulated kinase (ASK1), nuclear factor-κB-inducing kinase (NIK), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways [12,25,32] ... lungs were lavaged and lung tissue was prepared for pathological examination or measurement of EBD extravasation, MPO activity, and kinase activation Oxygen was fed into the inspiratory port of...