những vấn đề việt nam có thể đề xuất trong vòng đàm phán doha liên quan tới luật lệ của wto về chống bán phá giá
Báo cáo y học: "JNK suppression is essential for 17b-Estradiol inhibits prostaglandin E2-Induced uPA and MMP9 expressions and cell migration in human LoVo colon cancer cells" ppsx
... (Figure 1B) was induced following PGE2 (10-6M) treatment within h, and was maintained up for 24 h The quantitative results showed that uPA was significantly increased by approximately 2.47-fold within ... PGE2-induced motility of LoVo colon cancer cells derived from male origin The above study findings strongly suggest that estrogen treatment is an important program against the development of colon ... in colorectal cancer World J Surg 2002, 26(7):767-771 12 Seetoo DQ, Crowe PJ, Russell PJ, et al: Quantitative expression of protein markers of plasminogen activation system in prognosis of colorectal...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
... Ó FEBS 2004 Dynamic regulation of HO-1 expression in RPE (Eur J Biochem 271) 3077 visible light [22] and in cultured ... (Stratagene, La Jolla, CA, USA), then cloned into pCR-bluntII-TOPO (Invitrogen, Carlsbad, CA, USA), and named pCR-hHO1 RT-PCR was performed for preparation of the human TGF-b1 cDNA First strand cDNA was ... solution, amplified fragment was cloned into pGEM-Teasy vector (Promega, Madison, WI, USA), and named pGEM-hTGFb1 SP6 RNA polymerase was used for transcription of RNA probe from pCR-hHO1 Renilla...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
... When we examined MIG-17–GFP expression along developmental stages using Monoclonal 2, we detected strong bands corresponding to the activated form during L3 and L4, weak bands in L2 and the adult ... with Leu– Leu did not affect the processing, whereas replacement of Lys202–Lys203 with Leu–Leu strongly inhibited it [17] It is possible that MIG-17 with Leu–Leu in place of Arg205–Lys206 is cleaved ... two of the six hybridomas was inhibited by addition of the neo-epitope These two antibodies were named Monoclonal and Monoclonal Frozen sections were prepared as previously described [33] After...
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Báo cáo khoa học: Cas utilizes Nck2 to activate Cdc42 and regulate cell polarization during cell migration in response to wound healing docx
... selected fields were evaluated for directional protrusions in each of three independent experiments To quantify the protrusions’ length, the distance of the leading edge from the nuclei of wound edge ... Gelkop S, Shin NY, Hanks SK, Pawson T & Mayer BJ (2006) Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B Proc Natl Acad Sci USA ... 26 Hall A & Nobes CD (2000) Rho GTPases: molecular switches that control the organization and dynamics of the actin cytoskeleton Philos Trans R Soc Lond B Biol Sci 355, 965–970 27 Nakamura N,...
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Báo cáo Y học: Bax-induced cell death in yeast depends on mitochondrial lipid oxidation doc
... lipids The rate of this decrease was strongly accelerated in mitochondria isolated from Bax-expressing cells (Fig 4) This supports the hypothesis that Bax strongly sensitizes mitochondrial lipids ... oxidation, namely a-tocopherol and resveratrol were also assayed: both compounds significantly decreased kinetics of Bax-induced cell death (Fig 7B) Taken together, these results strongly support ... strong promoter GAL1/10 (Fig 8A) However, under these conditions, yeast supports a massive cytochrome c relocalization [25], which is likely to interfere with the response of these probes, namely...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... undertook to investigate the roles of PAK1 activation in LPA-induced cell motility and present herein strong evidence that PAK1 activation is involved in LPAinduced FAK phosphorylation and human melanoma ... were fixed and stained with DiffQuik reagents (Dade Behring, Inc.) Chemotaxis was densitometrically quantified using EAGLESIGHT software v3.2 (Stratagene) for data analysis, as described previously ... Slack, J.K., Taylor, J.M & Weed, S.A (2000) Focal adhesion kinase: a regulator of focal adhesion dynamics and cell movement Oncogene 19, 5606–5613 14 Abedi, H & Zachary, I (1997) Vascular endothelial...
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cell migration in inflammation and immunity
... into four sequential steps: tethering, triggering, strong adhesion, and migration The selectin family of adhesion molecules mediates tethering; strong adhesion is mediated by the integrin family, ... and Quantification of Interacting Cells The described experimental setting reproduces some of the physiological behaviors of homing lymphocytes, including tethering, rolling, and sticking Quantitative ... can be quantified To limit the description to chemokine-triggered integrin-dependent rapid adhesion, we will focus only on stable arrest analysis Integrin-Dependent Rapid Adhesion 23 Quantitative...
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Báo cáo y học: " Vasoactive intestinal peptide inhibits TNF-α-induced apoptotic events in acinar cells from nonobese diabetic mice submandibular glands" docx
... and exocrine secretion, VIP was postulated as a trophic factor for acini [17] and it has shown strong anti-inflammatory properties in several models of chronic autoimmune and immune-mediated inflammatory ... (ECL; Pierce, Rockford, IL, USA) Densitometric analysis of protein levels was performed with ImageQuant software (GE Healthcare, Chalfont St Giles, UK) RNA extraction and PCR amplification of cDNA...
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Báo cáo y học: "Hypoxia upregulates angiogenesis and synovial cell migration in rheumatoid arthritis" docx
... potential DNA contamination, RNA was treated using DNAse with RNasin (Ambion Ltd, Huntingdon, UK) Quantification of the RNA yield from each sample was carried out at 260 nm on a spectrophotometer ... primers were re-designed and revalidated Exon-spanning PCR primers (MWG, Ebersberg, Germany) for quantitative PCR were designed using Primer Software and UCSC Genome Bioinformatics [26], and are ... VEGF/ PlGF heterodimer No VEGF, PlGF or VEGF/PlGF protein was detected in the filtrates For mRNA quantification, the ABI Prism 7700 Sequence Detection System (Applied Biosystems, Foster City, CA,...
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Báo cáo y học: "Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo" doc
... images were captured by Gel Doc™ gel documentation system (Bio-Rad, USA) and intensity was quantified using Quantity-One software version 4.62(Bio-Rad, USA) In vivo tumor-growth inhibition studies ... filter for orange-fluorescent J-aggregate at hyperpolarized membrane potentials To measure the quantitative change of mitochondrial potential, we applied JC-1 with fluorescence plate reader Briefly, ... had green fluorescence (Figure 2A) JC-1 staining was used to determine mitochondrial integrity To quantify the change of mitochondrial potential, we applied JC-1 with fluorescence plate reader The...
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Báo cáo y học: "Directed cell migration in the presence of obstacles" doc
... cells and the obstacles under consideration The dynamics of cell movement very close to the obstacle surface are also influenced by short-range hydrodynamic interactions between the two In the interest ... is deferred to the last section of this article We shall now quantify the efficiency of chemotactic obstacle avoidance A convenient quantity to calculate is the capture radius rcap For a cell ... the capture probability separates two distinct regimes of dynamical behavior: (i) the chemotaxis-dominated regime in which cells strongly follow the chemical gradient (ii) the diffusion-dominated...
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Genetic analysis of the role of ARP2 3 complex in border cell migration in drosophila melanogaster
... expected to migrate (C) The quantification of border cell migration The upper panel shows how to quantify the border cell migation in stage The middle panel is the quantification of migration ... Red, WGA.(G) Quantification of number of nurse cells Scale bars, 20 µm 2.3 FRT-l(2)SH 1750 homozygous border cells show migration defect FRT-l(2)SH1750 is the second mutant line showed strong border ... border cells showed strong border cell migration delay Mutant border cell clusters were rare and a total of 14 full border cell clones at stage were recovered 12 of them showed strong migration delay...
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Cancer cell migration in 3d collagen gel matrix system
... migration through in vitro connective tissue models 1.2 Quantitative in vitro 3D cell migration assays Here we will analyze the rationale of using quantitative in vitro 3D cell migration assays for ... track cell movement 13 Chapter Introduction in 3D and quantitatively analyze cell speed and directionality Secondly, using this model, we will quantitatively evaluate drug effects on cell migration ... understanding cancer metastasis We will then unveil why anti-metastasis drug research requires quantitative in vitro three-dimensional (3D) cell migration assays Finally we will discuss the reasons...
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CHARACTERISATION OF DRUG INDUCED CELL DEATH IN MYCOBACTERIUM SMEGMATIS
... 3.5.2 Kanamycin treatment: E coli produces hydroxyl radicals in response to kanamycin This is observed in the Kohanski study and by us during our assay development experiments Since, Kanamycin ... ofloxacin isoniazid, ethambutol, and kanamycin All drugs were purchased from Sigma-Aldrich 25 The fluoroquinolones were prepared at a concentration of 0.5mM Kanamycin, isoniazid and ethambutol were ... 3.5 Hydroxyl radicals in response to Isoniazid, Ethambutol and Kanamycin Different concentrations of Isoniazid, Ethambutol and Kanamycin were applied to cell cultures and hydroxyl radicals were...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... Dynatech, MeLean, VA, USA) 96-well plate reader at a wavelength of 450 nm Cytokine levels were quantified by comparison with standards The sensitivity of IL-1b and IL-6 was < 0.1 and < 0.7 pgÆmL)1, ... the cells treated with ngÆmL)1 of SEC1 for 24 h The NF-jB-DNA binding activity (lanes 2–8) was quantified by densitometry The time-course groups were compared with the Ctl group to obtain the ... necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome Cold Spring Harb Symp Quant Biol 51 Part 1, 611–624 Spink J, Cohen J & Evans TJ (1995) The cytokine responsive vascular...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
... parent cell line with PYM over a period of years At the beginning of induction, cell growth was strongly suppressed However, at the end of induction, Tca8113 ⁄ PYM cells exhibited a stable growth ... dickkopf homolog (DKK1) Table Genes with upregulated expression in Tca8113 ⁄ PYM cells No Gene name GenBank ID Ratio Gene product 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 CA9 ... 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 Gene name GenBank ID Ratio Gene product PTGES FBN2 MAGEB2 RBP4 GALNT10 NM_198797 NM_001999 NM_002364...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
... the cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations Quantification of cell death Apoptosis was investigated by Annexin V labeling using a Sigma-Aldrich ... according to the manufacturer’s instructions Annexin V–fluorescein isothiocyanate (FITC) is used to quantitatively determine the percentage of cells within a population that are actively undergoing ... with Annexin V–FITC ⁄ propidium iodide Annexin V-positive and propidium iodide-positive cells were quantified using a FACScan FEBS Journal 275 (2008) 2096–2108 ª 2008 The Authors Journal compilation...
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Báo cáo Y học: Requirement of caspase and p38MAPK activation in zinc-induced apoptosis in human leukemia HL-60 cells potx
... was observed in Zn-treated cells [37–39] Intracellular Zn exists as fixed pools of Zn or as more dynamic and labile Zn pools, which are thought to be associated with the regulation of apoptosis by ... homolog 1, a novel cysteine protease similar to CPP32 J Biol Chem 271, 1825–1828 57 Tewari, M., Quan, L.T., O’Rourke, K., Desnoyers, S., Zeng, Z., Beidler, D.R., Poirier, G.G., Salvesen, G.S & ... of apoptosis with change in intracellular labile Zn (II) using Zinquin [(2-methyl-8-toluenesulphonamido-6-quinolyloxy) acetic acid], a new specific fluorescent probe for Zn (II) Biochem J 296, 403–...
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Báo cáo Y học: SF2/ASF protein inhibits camptothecin-induced DNA cleavage by human topoisomerase I potx
... preparation used in the cleavage assay as Ôphosphorylated SF2/ASFÕ The amount of htopo I and SF2/ASF was quantified by densitometric scanning of SDS-polyacrylamide gels using BSA as a standard DNA cleavage ... electrophoresis for 1.5 h at 100 V Gels were stained with ethidium bromide and the reaction was quantified by densitometric scanning of negatives of photographed electrophoretic gels Relaxation...
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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc
... key elements was quantitated by immunoassays as described in the Experimental procedures Phosphorylation of all samples except JNK were analyzed using immunoassays whereas JNK was quantitated using ... by a double outline around the name together with the corresponding functionally related symptom of SEB All proteins are indicated by a single outline around the name Symbol ‘X’ indicates inhibition ... to a profile that is specific for SEB [18] Even though RT-PCR is considered to be a semi-quantitative gene quantification method, the results observed are significant for two reasons The purpose of...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose