neuroticism genetic mapping of
báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... region of LG5 of both F1 (NA6 × AU6) parental maps (Fig 3, 4, 5, 6) Comparative genetic mapping based on R gene loci Genetic mapping facilitated map integration between trait-specific ryegrass genetic ... xlpcat-561cg xlpffta.1 Figure Genetic linkage maps of LGs 1–4 from the NA6 parental genotype of the F1(NA6 × AU6) cross Genetic linkage maps of LGs 1–4 from the NA6 parental genotype of the F1(NA6 × AU6) ... xlpthc.2 xlpmads4.2 Figure Genetic linkage maps of LGs 5–7 from the NA6 parental genotype of the F1(NA6 × AU6) cross Genetic linkage maps of LGs 5–7 from the NA6 parental genotype of the F1(NA6 × AU6)...
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báo cáo khoa học: " Genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid" docx
... introgression of a small fraction of the wild genome species while keeping the genetic background of the cultivated is a good mean to explore the largely untapped reservoir of useful alleles of interest ... application of molecular breeding approaches for the genetic improvement of cultivated peanut Up to date, few genetic linkage maps have been published in Arachis At the diploid level, three genetic ... due to the low level of polymorphism between cultivated peanut varieties, QTLs have been detected attesting of the interest of molecular breeding tools in genetic improvement of peanut Burow et...
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Báo cáo y học: "Genetic polymorphism of p53, but not GSTP1, is association with susceptibility to esophageal cancer risk – A Meta-Analysis"
... reached a consensus on all of the items Data extracted from these articles included the first author’s name, year of publication, country of origin, ethnicity, number of cases and controls, genotype ... DSTPLAN4.2 software, in order to assess the probability of detecting an association between RANTES polymorphisms and asthma at the 0.05 level of significance, assuming a genotypic risk of 2.0 and ... located in epithelial cell of the mouth throat or lungs and adenocarcinoma is composed of cells of glandular tissue Over the past decades, many changes in the prevalence of esophageal cancer have...
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Tài liệu Báo cáo khoa học: Mapping of the epitope of a monoclonal antibody protecting plasminogen activator inhibitor-1 against inactivating agents pptx
... measurement of the increase in absorbance at 405 nm The specific inhibitory activity of PAI-1 was calculated from the amount of PAI-1 that had to be added to inhibit 50% of the uPA The halflives of the ... PDB VIEWER Results Measurements of the effects of Mab-1 or Mab-5 and neutralizers on the specific inhibitory activity of PAI-1 Epitope mapping To measure the effects of antibodies and inactivators ... peptide In the presence of Mab-1, the half-life for latency transition of PAI-1 was increased by a factor of 1.5 The half-lives in the presence of Mab-1 and in the presence of VN were indistinguishable...
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GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH pptx
... characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork arrest at Tus-Ter complexes have been determined This and a brief description of the current state of ... activation domain of Gal4 of yeast (pGAD424-X) A suspected interacting Genetic Manipulation of DNA and Protein – Examples from Current Research Fig Crystal structure of Tus-Ter complex of E coli and ... structure of the cofactor binding-site of Hfx mediterranei GlcDH (Fig 6) indicates the spatial location of the residues mutated here and the interaction of R207 and R208 with the 2’-phosphate group of...
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Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx
... different parts of MUC5AC Fig Mapping of the 45M1, 166M1 and 2-12M1 epitopes on the C-terminal cysteine-rich part of human MUC5AC The upper part of the figure shows a schematic representation of full-length ... recombinant C-terminal cysteine-rich part of human MUC5AC A schematic picture of the recombinant C-terminal cysteine-rich part of human MUC5AC is given in the upper part of the figure The GDPH-cleavage site ... cleavage fragment The lower part of the figure shows an alignment of the N-terminal MUC5AC sequences of M-MUC5AC-CH-long and M-MUC5AC-CHshort and the corresponding sequences of human, rat and mouse MUC5AC...
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Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx
... Comparison of the Mg2+-dependent activity of the unmodified deoxyribozyme 10–23 with that of thio-substituted deoxyribozyme RP-P5 in the presence of mM MgCl2 Time course of cleavage reaction of a chimeric ... ratio of kMg of obs the reference unmodified enzyme to kMg of the particular obs FEBS Journal 274 (2007) 1062–1072 ª 2007 Centre of Molecular and Macromolecular Studies of the Polish Academy of Sciences ... °C c Mg k rel ¼ the ratio of the kobs values of modified deoxyribozyme to unmodified deoxyribozyme, in the presence of Mg2+ e k Mn ¼ the ratio rel of the kobs values of modified deoxyribozyme to...
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Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
... subsite mapping of other a-amylases found in the literature Evaluations of subsite maps of rice and barley a-amylases are thus also presented MATERIALS AND METHODS SUMA software: subsite mapping of ... Hiromi, K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions Evaluation of subsite affinities of exo-enzyme Biochem Biophys ... Fig Structure of the computer program Subsite mapping of PPA–‘five subsite model’ is confirmed Porcine pancreatic a-amylase is one of the most exhaustively studied model enzymes of mammalian a-amylases...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
... description of antibody-LPS binding examined by STD NMR spectroscopy and shows the advantages of this direct approach for the purpose of relatively quick and direct epitope mapping LPS each of wild-type ... substituents, respectively, of (s), 3-E (n), 3-Z (e), and (h) are shown The two signals of the N-methyl groups of showed identical behaviour A signal of the C-methyl group of the acetamido group in ... groups of 2-E; NMe, N-methyl group of the isomers of 3; NAmCH3 and NAcCH3, C-methyl group of the acetimidoylamino and acetamido substituents, respectively at which the N-methyl signals of already...
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Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt
... for the genetic basis of b2GPI plasma variation, but the exact molecular basis of this variation remains largely unknown b2GPI is suggested to regulate thrombin inactivation by heparin cofactor ... gene of the pGL3-basic reporter vector The sequence of each construct was verified by sequencing (data not shown) Figure 1A shows the expression of deletion mutants in COS-1 cells 5¢ deletions of ... characterization of APOH promoter Fig ECR Browser conservation profile of the 5¢ region of APOH (1418 bp; )1375 ⁄ +43 nucleotides from the translation initiation codon ATG) Sequence elements of significant...
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Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
... dissociation was observed with the A8B8 isoform of GAPDH leading to the A2B2 isoform with a higher NADPHdependent activity [39] By analogy with the CTE of the isoform of GAPDH [15,37], we hypothesize ... changes in activities of the enzymes is thus of crucial importance to find a general mode of light regulation of the Calvin cycle in all photosynthetic organisms GAPDH is composed of four identical ... et al on the A2B2 isoform of GAPDH [24,37], formation of a disulfide bridge in the oxidized CTE prevents the interaction between the 2¢-phosphate of NADPH and the side chain of Arg77 (spinach numbering,...
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Genetic Manipulation of DNA and Protein – Examples from Current Research Edited by David Figurski potx
... characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork arrest at Tus-Ter complexes have been determined This and a brief description of the current state of ... activation domain of Gal4 of yeast (pGAD424-X) A suspected interacting Genetic Manipulation of DNA and Protein – Examples from Current Research Fig Crystal structure of Tus-Ter complex of E coli and ... structure of the cofactor binding-site of Hfx mediterranei GlcDH (Fig 6) indicates the spatial location of the residues mutated here and the interaction of R207 and R208 with the 2’-phosphate group of...
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Báo cáo khoa học: Mapping of the active site of glutamate carboxypeptidase II by site-directed mutagenesis potx
... decrease of both the Km and kcat values The mutation of Gly518 to Pro led to a slight increase of the Km value and one order of magnitude decrease of the kcat value (Table 2) Analysis of inhibitor ... Stable transfection of Drosophila S2 cells and large scale expression of mutant forms of rhGCPII Conclusions In conclusion, we report a detailed analysis of the active site of glutamate carboxypeptidase ... QM ⁄ MM structure of NAAG bound in the active site of GCPII.The carbonyl group of L-aspartate from NAAG (depicted in green) accepts a hydrogen bond ˚ from the hydroxyl group of Tyr552 (distance...
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Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx
... chaperone that aids in the refolding of misfolded proteins Loss of function of protein homeostasis genes also causes accumulation of damaged proteins and activation of gpdh-1 expression Green arrows ... powerful model system for forward genetic analysis Mutagenesis and genetic screening allows the unbiased identification of genes underlying a biological process of interest, allows genes to be ordered ... injection of double stranded RNA (dsRNA), or at the population level by feeding worms dsRNA-producing bacteria Libraries of dsRNA feeding bacteria are now available that allow for over 90% of the...
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Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx
... exon of the zebrafish B-FABP gene The 50 lL reaction contained 1· PCR buffer, 1.25 U of Taq DNA polymerase (MBI Fermentas), 1.5 mM MgCl2, 0.2 mM of each dNTP, 0.2 lM of each primer and lL of linker-ligated ... and other members of the FABP family, that the N-terminal halves of these proteins are more highly conserved than their C-terminal halves [27] Mapping of the initiation site of transcription for ... end of the mature B-FABP mRNA The 5¢ RACE product contained a 166 bp sequence corresponding to a portion of exon including the 5¢ UTR of the Fig Product of 5¢ RLM-RACE derived from the 5¢ end of...
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Báo cáo khoa học: Mapping of chorismate mutase and prephenate dehydrogenase domains in the Escherichia coli T-protein doc
... quantity of papain T-protein (20 lg) was dissolved in 100 lL of 0.1 M NH4Ac, 0.004 M EDTA, 0.01 M cysteine (pH 6.8) and 0.4 lL of 0.1 mgÆmL)1 (1 : 50 ratio) or lL of 0.01 mgÆmL)1 (1 : 1000 ratio) of ... respectively One unit of enzyme was defined as the amount of enzyme required to produce lmol of product per minute at 37 °C Specific activity was expressed as units per mg of protein Expression ... absence of NAD+, the CM activity of fragments T/1–88, T/1–94 and T/1–108 was unaffected by Tyr at concentrations up to 300 lM The CM activity of fragment Table CM and PDH activities of cloned...
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Lập bản đồ di truyền (genetic mapping) potx
... lập đồ vật lý (physical mapping) liên quan tới việc sử dụng kỹ thuật phân tử di truyền tế bào để xác định vị trí vật lý...
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the genetic manipulation of plants
... cell genetics of higher plants Annual Review of Genetics 8: 267–78 17 Kleinhofs, A & Behki, R (1977) Prospects for plant genome modification by nonconventional methods Annual Review of Genetics ... plant Lack of organ specificity in gene expression has been a major problem for 34 • SEEDS OF CONCERN genetic engineers A prime example of this kind of imprecision is the production of a toxic ... possible impacts of genetically modified plants on genetic diversity, the environment, human health, or human society, then here is a balanced source of information Uncritical proponents of genetically...
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unsw press seeds of concern the genetic manipulation of plants
... cell genetics of higher plants Annual Review of Genetics 8: 267–78 17 Kleinhofs, A & Behki, R (1977) Prospects for plant genome modification by nonconventional methods Annual Review of Genetics ... plant Lack of organ specificity in gene expression has been a major problem for 34 • SEEDS OF CONCERN genetic engineers A prime example of this kind of imprecision is the production of a toxic ... possible impacts of genetically modified plants on genetic diversity, the environment, human health, or human society, then here is a balanced source of information Uncritical proponents of genetically...
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three dimensional mapping of optical near field of a nanoscale bowtie antenna
... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its ... Three-dimensional mapping of the near-field intensity can be acquired by turning off the feedback and scanning the probe over the sample at a manually set height This approach allows the measurement of the...
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