... 1,25-(OH)2D3 on podocyte apoptosisand podocytopenia The present study aimed to investigate the effects of 1,25-(OH)2D3 on podocyte apoptosisand podocytopenia induced by adriamycin (ADR) and the potential ... Bax/Bcl-2 ratio and finally limiting glomerular cell apoptosis Our study demonstrates the protective effect of 1,25-(OH)2D3 on podocyte apoptosis by down-regulating Fas, FADD and Bax and up-regulating ... collagenase and culture Early cellular outgrowths at 5-7 days were selectively removed by a cylinder cloning technique Cells were replated, and plates growing pure colonies were then expanded and identified...
... of TJ proteins such as claudin-1 and claudin-4 induces apoptosisand suppresses metastatic potencies [10,11] Occludin has also been reported to induce apoptosisand apoptotic sensitization, which ... occludin (OccWT) and variant occludin in apoptosisand invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosisand the reduction ... concentration increases apoptosisand decreases invasiveness On the basis of the differences between OccWT and OccDE9 with regard to mitochondria-dependent apoptosisand reduction of invasion,...
... microvilli and junctional complexes, and blebbing can occur In several cases, an intense endocytosis is observed, and this probably Fig Multiple pathways to apoptosis, both dependent and independent ... activation (GluRd2-Lc) Yue and collaborators [113] showed that GluRd2, nPIST and beclin-1 interact, and that autophagy can be induced by nPIST and beclin-1 synergy and by the mutated GluRd2-Lc, ... section will examine its relationship with apoptosisApoptosis vs autophagy Notwithstanding the abundant evidence for a role for both autophagy andapoptosis in various diseases, their interplay...
... reduced tumour proliferation, and tumour regression, accompanied by apoptosis [40] An understanding of how and under what circumstances MEK inhibition can promote apoptosis may permit a more targeted ... variants, BIMS, BIML and BIMEL (BIM-short, BIM-long and BIM-extra long, respectively), are all cytotoxic and subject to different modes of regulation by various prodeath and prosurvival signalling ... loss of ERK1 ⁄ and ⁄ or PKB signalling and, as a consequence, promote increased expression of BIM and BIM-dependent cell death in tumour cells Here we review recent advances in understanding BIM...
... kinases ATM and ATR ATR and ATM in turn phosphorylation-dependently activate the downstream checkpoint kinases Chk1 and Chk2, respectively, and the tumour suppressor p53 Furthermore, ATM and ATR ... we focus on the apoptotic p53, p73 and HIPK2 in apoptosis signalling routes of the DDR regulated mainly from the cell nucleus and the key molecules p53, p73 and homeodomain-interacting protein ... cisplatin) and has been shown to drive the expression of apoptotic p53 target genes, such as p53-upregulated modulator of apoptosis (Puma), p53 regulated apoptosis- inducing protein (p53AIP1) and breakpoint...
... caspase-1, caspase-9 ⁄ HIP-1 & HIPPI mediated apoptosisand transcription caspase-6, and caspase-3 Cleavage of Bid and release of cytochrome c and apoptosis- inducing factors from the mitochondria ... apoptosisand transcription Hippi Also, expression of the mitochondrial-coded genes ND1 and ND4, the nuclear genome-coded mitochondrial genes SDHA and SDHB and the antiapoptotic genes BCL-2 and ... in the regulation of cytoskeletal and endocytic processes HIP-1 and its interacting partners – roles in apoptosisand survival Various pathways followed during apoptosis have been reviewed recently...
... the androgen receptor to control cellular processes such as proliferation, apoptosisand other metabolic events in prostate cancer [14] Taken together, a combination of gene amplification and androgen ... 24 h and (D) 48 h (D) Overlay of (A) and (C) TPD52 downregulation activates caspase activity and increases the loss of mitochondrial membrane potential (E, F) Showing the relative caspase-3 and ... as TPD52 and its locus has been mapped on chromosome 8q21 [4,5] Its encoding gene is also referred to as PrLZ and is a member of the tumor protein D52 (TPD52) gene ⁄ protein family and is designated...
... necrosis and natriuretic peptides are well known indicators of cardiac dysfunction and filling pressures In addition, certain other biomarkers are known to be indicators of inflammation and apoptosis, ... Fas/Fas ligand system, shows increased activity in HF patients and correlates to disease severity [6,7] To briefly summarize, soluble Fas-Ligand (sFas-L) binding to membrane-bound Fas triggers apoptosis, ... manufacturer protocol and using standard curves and software The kits for NTproBNP (fmol/ml) were purchased from ALPCO Diagnostics, Salem, New Hampshire; for IL-6 (pg/ml) and TNFα (pg/ ml) from...
... degenerative disorders [8,9] Apoptosis is a physiological process and is a highly selective way to eliminate aged and injured cells In addition to internal pathways that trigger apoptosis mainly in response ... of the apoptosis- inducing receptor Fas in periarticular tenocytes of patients with OA and studied their susceptibility to apoptosis in the presence or absence of recombinant human FasL and TNF-α ... Germany) and reverse transcribed using random hexamer primers Primers and FAM-TAMRA-labelled probes for the real-time PCR were purchased as a predeveloped assay from Applied Biosystems and used...
... contributions YZ and AY are researchers working in cancer biology and carried the study QL and YB undertook the Statistical analysis QZ along with MZ designed the work and interpreted the results QZ and MZ ... western blot and stained by both anti-Bcl-2 (BH3) and anti-Bax (6A7) antibodies, express conformational change of Bcl-2, which may play a role in arsenic trioxide-induced apoptosisand Bax activation ... arsenic trioxide on early and late apoptosis/ necrosis of SGC7901 cell (* represents p < 0.05 compared to the black control group and arsenic trioxide treated groups) at 24 hours and the difference compared...
... the cleavage of proteins during apoptosisand necrosis because its cleavage products in Jurkat cells undergoing apoptosis (89 kDa) and undergoing necrosis (60 and 50 kDa) are well established ... nuclear proteins in apoptosisand necrosis revealed by autoantibody probes Cell Death Differ 1998, 5:183-190 Utz PJ, Anderson P: Posttranslational protein modifications, apoptosis, and the bypass ... in apoptosisand in necrosis, prompting us to examine whether specific Golgi proteins are targeted for proteolysis in these modes of cell death Golgi autoantigens are cleaved during apoptosis and...
... generated and provided the recombinant chimeric TRAIL protein PDR conceived of the study and participated in its design and coordination and helped to edit the manuscript All authors have read and ... USA) plasmid, and expressed protein was purified using the His-band Resin and Buffer Kit (Novagen) [13] Analysis of the purified rTRAIL protein demonstrated that both trimeric and dimeric TRAIL ... expression of TRAIL/Apo-2 ligand in activated mouse T and B cells Eur J Immunol 1998, 28:1492-1498 Jeremias I, Herr I, Boehler T, Debatin KM: Trail/Apo-2-ligandinduced apoptosis in human T cells...
... rejection level of 5% Data are expressed as mean ± standard deviation Results Expression of CD95 and CD95 ligand To determine whether CD95 and its ligand were involved in induction of cell death in ... of apoptosisand that of necrosis were increased by antiCD95 treatment, and these increases were diminished by inhibition of p38 MAPK (Figure 3b) Thus, both CD95 induced chondrocyte apoptosisand ... [lower right] and panel c [lower right]); and y axis is FL2-H (log), and fluorchrome is red for PI (panel b [upper left] and panel c [upper left]) Living cells are shown to the lower left and double...
... anterior and posterior commissure and covered the whole brain in all sequences Conventional T1-weighted spin-echo, fluid-attenuated inversion recovery and dual (fast spin-echo proton density and T2-weighted) ... MTR, peak height and peak location Nonparametric Mann–Whitney tests were performed to compare clinical and grey and white matter MTR histogram parameters between patients with and without IgM ... aCLs and 13 for Lac, yielding a comparison of nine versus nine patients for IgM aCL, nine versus nine patients for IgG aCL, and 13 versus five patients for Lac For anti-dsDNA and anti-ENA, four and...
... tables and figures and has been involved in type of packaging likewise in the submission process PCL has been involved in conception and design of the study and in drafting the manuscript and has ... are 1) non-homologous end joining (NHEJ) and homologous recombination repair (HRR) [29,30], and 2) cell-cycle checkpoints that provide time for repair andapoptosis [31] Depending on the severity ... spontaneous apoptosis) ; and the coefficient b defining the slope of the curve a and b followed a normal distribution (Kolmogorov-Smirnov test, p > 0.05) Mean of b was 7.93 ± 2.68 standard deviation...
... eagle medium (Gibco, Grand Island, NY) containing 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Grand Island, NY), penicillin (100 U/mL) and streptomycin (100 mg/mL), and they were maintained ... the expression of b1- and b2-ARs was determined at both the mRNA and protein level in SGC-7901 and BGC-823 cells by RT-PCR and western blot Our results showed that b1- and b2-adrenergic receptors ... the data and plotting of the figures were performed with the aid of software (Origin Version 7.5 and SPSS Version 13.0) Results Expression of b1- and b2-adrenergic receptors in SGC7901 and BGC-823...
... contributions PCS and CT carried out the experiments and drafted the manuscript BHC, CWL, and CPY participated in the design of the study and performed the analysis JSJ conceived of the study, and participated ... concentration- and time-dependent manners SAHAresistant cancer cells express high levels of cyclin A, Ki67, p53, survivin, and p21 Discussion HDACi inhibits acetylation of histones H3 and H4 and induce ... concentrations Specifically, SAHA induced apoptosisand sub-G1 arrest in 320 HSR colon cancer cells by inhibiting the protein expression of anti -apoptosis proteins, survivin and Bcl-xL Multiple mechanisms...
... DCM and ICM VEGF treatment maintained mitochondrial fission and fusion balance, increased the expression of inhibiting apoptosis proteins OPA1 and Bcl-xL, decreased the expression of promoting apoptosis ... in cardiac hypertrophy and normal cardiac function A, Representative transthoracic M-mode echocardiogram for sham and TAC mice B, IVSd, LVPW, and LVFS data between sham and TAC mice Cardiomyocyte ... mitochondrial apoptosis pathway regulation proteins Bcl-xL and Bax were also detected by western blotting The up-regulation of Bcl-xL and down-regulation of Bax expression further substantiated the anti-apoptosis...
... Immunohistochemical analysis The apoptosis downstream factor, CASPASE-8 and CASPASE-3, plays vital role in both cell intrinsic and extrinsic apoptosis CASPASE-8 and CASPASE-3 was induced in the ... tbx5-MO-treated embryos apoptosis related genes either anti -apoptosis related gene (bcl-2) or pro -apoptosis related genes (bad and bax) were all transcriptionally activated, and so were the cell ... markers (cdk2 and pcna) and cyclin kinase inhibitors (p27 and p57) In protein level, remarkably plentiful production of apoptosis- related proteins including CASPASE-3, CASPASE-8, and BAD were...