neuronogenesis and stem cells in normal brain aging

Báo cáo hóa học: " Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats" pptx

Ngày tải lên : 18/06/2014, 16:20

... in brain infarct area and differentiation into endothelial-cell phenotype The IHF imaging (A) (400×) results showing double stains of CM-Dil and DAPI-positive cells (white arrows) in the brain ... Angiogenesis and Neurogenesis Figure Oxidative index in brain infarct area Western blotting showing notably increased oxidative index, protein carbonyls, in BIA of group compared with groups and on ... several candidates of stem cells, including embryonic stem cell, neuron stem cells, bone marrow-derived mesenchymal stem cell, and peripheral blood-derived stem cells have been frequently investigated...

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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

Ngày tải lên : 07/03/2014, 04:20

... these cells Thus, we studied the uptake and release of creatine in EAC and S180 cells as a model system Figure shows that, if the cells were incubated for h in the uptake medium containing creatine, ... estimation of proline and ornithine J Biol Chem 199, 91–95 45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956) Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methyl-guanidine ... membrane occurs in malignant cells, and needs further investigation The presence of both AGAT and GAMT in EAC and S180 cells and in sarcoma tissue indicates that the upregulated proteins are entirely...

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Stem Cells in Human Reproduction Basic Science and Therapeutic Potential Second Edition pot

Ngày tải lên : 22/03/2014, 20:21

... embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells by co-culture ... pivotal role in translation initiation by binding eIF4E and eIF4A, by forming a bridge between the mRNA and the ribosome, and by binding PABPC1 to facilitate translation of poly(A)-containing mRNAs ... stored mRNAs RINGO/Spy protein is absent in the oocyte cytoplasm, while its mRNA is maintained in a dormant state This is achieved by binding of Pumilio-2, an RNA-binding protein initially described...

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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

Ngày tải lên : 18/06/2014, 16:20

... recruited into the study after understanding and signing informed consent Exclusion criteria included: systemic immune-compromised state, ongoing infection or disease conditions, and significant abnormalities ... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... stained with Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic media and confirmed calcium deposition A and B: Oil red O stain, C and D: Alizarin red S stain, ... New canine spinal cord injury model free from laminectomy Brain Res Brain Res Protoc 2005, 14, 171-180 11 Gimble J, Guilak F Adipose-derived adult stem cells: Transplantation of ASCs in spinal...

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báo cáo khoa học: " Stem cells in clinical practice: applications and warnings" pot

Ngày tải lên : 10/08/2014, 10:20

... such as intestine [52] and from the ectodermal tissues including skin [53], deciduous teeth [54] and nerve tissue [8,9,55,56] ASCs originate during ontogenesis and remain in a marginal area in a ... islets and reintroduce physiological secretion of human insulin AHSCT improves b -cells function and frequently decreases the exogenous insulin need [20] or induces a persistent insulin independence ... uncertainties must be borne in mind during the independent peer review of the preclinical data Only when the compelling preclinical data are available, careful and incremental testing in patients...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Ngày tải lên : 11/08/2014, 20:21

... were fixed in formalin (4%) until further procession Formaline-fixed microspheres were dehydrated in increasing ethanol concentrations (50%, 75%, 90% and 100%) and embedded in paraffin (Paraplast ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... slides, deparaffinized with xylol and rehydrated in decreasing ethanol concentrations Samples were stained with alizarine red solution (2%) to detect calcium and counterstained with toluidine blue,...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington DC; 1981 Landry DW, ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving...

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Pulmonary tuberculosis associated with increased number and percentage of natural killer and B cells in the peripheral blood pot

Ngày tải lên : 29/03/2014, 03:20

... patients in this study Immunity against MTB comprises of a predominant cellular response and majority of researchers have dismissed the participation of B cells in defending against the infection B cells ... response against MTB by increasing the infection inoculum (Vordermeier et al., 1996; Maglione et al., 2007) In addition the protection of mice against MTB infection by administration of intravenous ... detected in patients with PTB in this study may therefore indicate the contribution of NK cells in the host immune response against MTB infection The immune response after MTB infection and disease...

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scientific american - 1995 11 - guardian cells in the brain

Ngày tải lên : 12/05/2014, 15:02

... macrophagelike cells responding to the damage would be residents of the brain tissue, not interlopers from the blood Finally, they examined the brain region containing the remains of the aíected nerve cells ... monocytes attracted to the developing nervous system by neurons that died during the sculpting of the brain and spinal cord But new findings contradict that view For instance, Jutta Schnitzer of the ... Its marks can be found in North Atlantic marine sediments, Scandinavian and Icelandic glacial moraines, and northern European and maritime Canadian lakes and bogs New England also cooled signiịcantly...

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báo cáo hóa học: "Kinematic aspects of trunk motion and gender effect in normal adults" pdf

Ngày tải lên : 19/06/2014, 08:20

... gender, normalized walking speed and BMI Therefore, after excluding the confounding effect of the normalized walking speed and body size, the most prominent gender difference in the kinematic ... at least in part, and care should be taken when interpreting the clinical implications Posterior tilting of the trunk (Tilt (G) graph in Figure 1) begins with the initiation of the single limb ... motion and transverse plane motion are possibly interconnected in three dimensional space This concurs with the findings of a previous study, in which coupling between lateral bending and axial...

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Báo cáo sinh học: " Research Article Estimation of Time-Varying Coherence and Its Application in Understanding Brain Functional Connectivity" potx

Ngày tải lên : 21/06/2014, 16:20

... perspective into the characteristics of time-varying spectrum via studying the properties of the corresponding kernel However, to maintain physical meaningness in time-varying spectrum and coherence, ... stationary neighborhoods (a) in the coordinates of two time instants and (b) in the coordinates of the center time location and the time index diﬀerence (1) The time-varying spectrum, as an energy ... W Gaetz, and H Zhu, “The Stockwell transform in studying the dynamics of brain functions,” in Proceedings of the International Workshop Pseudo-Diﬀerential Operators: Complex Analysis and Partial...

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Báo cáo khoa học: "Macrophages, myofibroblasts and mast cells in a rat liver infected with Capillaria hepatica" ppsx

Ngày tải lên : 07/08/2014, 20:23

... myofi- Capillaria hepatica infection in a rat liver 213 broblasts and mast cells were increased in animals with hepatic fibrosis and these cells might play a role in the formation of hepatic ... myofibroblasts and mast cells in the liver in the hepatic fibrous septa of an animal infected with C hepatica This finding is consistent with that previously seen in a rat model of CCl4-induced hepatic ... relationship and role of macrophages, myofibroblasts and mast cells in the hepatic septal fibrosis induced by C hepatica We hypothesize that a similar mechanism involving macrophages, myo-fibroblasts and...

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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C ... latipsoH gnihcaeT lacideM yranireteV ytisrevinU lanoitaN luoeS ta god lergnom gk 85 a fo noitces nairaseaC eht gnirud deniatbo saw BCU :noitcelloc lleC sCSM-BCU eninac fo snoitaraperP dehsilbatse saw ... yliad semit eerht tsael ta demrofrep saw noisserpxe reddalb launam ,dedeen fi dna ,tinu erac evisnetni na ni derotinom erew sgod eht ,noitarepo eht retfA h 21 retfa devomer neht dna ,erutus epyt...

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Báo cáo y học: "Expression of novel extracellular sulfatases Sulf-1 and Sulf-2 in normal and osteoarthritic articular cartilage" pdf

Ngày tải lên : 09/08/2014, 10:23

... processes Competing interests The authors declare that they have no competing interests Sulf-1 and Sulf-2 expression in murine temporomandibular joints joints Safranin O staining (a-c) and immunohistochemistry ... with safranin O staining (Figure 6a–c) and immunohistochemistry for Sulf-1 and Sulf-2 (Figure 6d–i) Histology showed thinning and reduced cell density in articular cartilage with increasing age ... levels in regions of developing cartilage and bone [37] The present study reports on the expression of Sulf in mature cartilage and changes with aging and OA In normal articular cartilage, Sulf-1 and...

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Báo cáo y học: "Mesenchymal stem cells in arthritic disease" pot

Ngày tải lên : 09/08/2014, 13:22

... delivered via either adenoviral infection or osmotic pumps in inhibiting bleomycin-induced increases in TNF-α, IL-1α, and trafficking of lymphocytes and neutrophils into the lung [56] These successful ... and IL-6, and secrete MMPs, leading to the systemic inflammation that eventually results in joint destruction [78,80] Pharmacological interventions aiming at reducing inflammation, including methotrexate ... Technology insight: adult stem cells in cartilage regeneration and tissue engineering Nat Clin Pract Rheumatol 2006, 2:373-382 Bianco P, Robey PG, Simmons PJ: Mesenchymal stem cells: revisiting history,...

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Báo cáo y học: "Anti-tumour necrosis factor therapy and B cells in rheumatoid arthritis" pptx

Ngày tải lên : 09/08/2014, 14:22

... these cells in the inflamed synovia and that blocking the effects of TNF decreases trafficking into and retention of B cells in the synovia, thereby increasing their proportion in the peripheral ... Leandro leucocytes into inflamed joints), angiogenesis and tissue destruction [7] However, TNF and a related cytokine, lymphotoxin alfa (LTα), also play an important role in the development and ... structures in the inflamed synovia [10] Souto-Carneiro and colleagues [1] suggest that the decrease in circulating pre-switch memory B cells in RA patients is due to the accumulation of these cells in...

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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx

Ngày tải lên : 10/08/2014, 09:22

... for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids as ... were stained with only haematoxylin Three pathologists (M.N., H.O., and T.N.) independently made a diagnosis using the μm sections stained with haematoxylin and 2 Immunohistochemical Staining The ... between LCNEC and LCC, and 453 in the overlap among three groups; SCLC contained a total of 1,096 including 362 unique, 100 in the overlap only between SCLC and LCC and the overlapped proteins described...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... of stem cells and for understanding their behaviour in physiological and pathological conditions Niches are local tissue microenvironments that maintain and regulate stem cells [52], Livraghi and ... systems Labeled cells retain both biological and proliferating activity, and are ideal for in vitro cell labeling, in vitro proliferation studies and long term, in vivo cell tracking In the current ... the underlying molecular mechanisms that link MSCs to the targeted inhibition of tumor cells Despite their distinct origins, stem cells and tumor cells share many characteristics[15,16] In particular,...

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