neurofilaments are required for radial growth
Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... sequence contains positive regulatory element(s) required for basal Prm3 activity Bioinformatic analysis of Prm3, using the matinspectorTM program [26], for transcription factor elements between )154 ... 16 000 g for 10 and the protein concentration of nuclear extracts were determined using the Bradford assay [57] For electrophoretic mobility supershift assays, nuclear extracts were prepared under ... Kin340; 5¢-dTGTCAGATGCAAATCACTAGAA-3¢) Note, only forward oligonucleotides are given and sequences of the corresponding complementary strands are omitted For electrophoretic mobility supershift assays,...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... Saccharomyces cerevisiae is an appropriate system for studying the components of coactivators that are required for their recruitment During growth on nonrepressing, non-inducing sugars such ... Tup-Ssn6 corepressor complex are differentially important for the repression of different target genes in fission yeast [45,46] Swi1 and Snf5 are known to be required for the structural integrity ... for comparison of wildtype and mutant strains Primers used for quantification of transcripts and promoter regions were for the most part designed using primer3 software [54] Primer sequences are...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... values were determined by a nonlinear least-squares fit of the initial velocity vs substrate concentration using a GRAFIT software package (Erithacus Software Limited) Large scale expression and purification ... sequence at the N-terminus of the protein was also shown to be required for the activity and/or secretion of the GCPII carboxypeptidase As for the N-terminally modified variants, the absence of the ... fold are cycled via the calnexin-calreticulinglucosidase I and II system within the ER lumen, providing space and time for the unfolded/partially folded proteins to acquire the correct 3D conformation...
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Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt
... mammalian Pig-M protein and its yeast ortholog Yjr013wp are required for addition of Man1 to GPI precursors [19], and the PIG-B ⁄ Gpi10 proteins for addition of Man3 [20–22] However, a candidate GPI-MT-II ... manner except that each growth medium was supplemented with mm ethanolamine and mm choline, and metabolic labeling was performed with 20 lCi [14C]ethanolamine for 23 h at 25 °C For radiolabeling ... grown in SGlyYE medium for days at 25 °C, then grown in SGalYE or SGlcYE medium for 16 h Cells were shifted to 25 °C or 37 °C for 20 and radiolabeled with 15 lCi [3H]inositol for h Radiolabeled lipids...
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Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... are known to be functional substrates for thiolase, but kinetic constants have been reported only for AcAc-SPP [3]; for example, Km and kcat are 73 lm and 469 ⁄ s for AcAc-SPP, whereas they are ... of thiolase are important for competent binding of the substrate Therefore, a calorimetric study was carried out to further investigate the importance of sulfur–sulfur interactions for the affinity ... thiolase at 25 °C The values and error estimates are calculated from separate measurements (three for the wild-type and two for C89A) Ka and Kd are the association and dissociation constants,...
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Báo cáo sinh học: " Transcription and translation of human F11R gene are required for an initial step of atherogenesis induced by inflammatory cytokines" doc
... plates were incubated at 37°C for hr Paraformaldhyde (4%), pH 7.4, was added to each well and incubation continued at 23°C for 15 The addition of paraformaldehyde, before washings, did not affect ... [29] reported a role for the F11R in the repair of the injured, inflamed endothelium, by showing that JAM-A/F11R molecules expressed on endothelial progenitor cells are required for the re-endothelialization ... applied to the media and the ECs were further incubated at 37°C for up to 24 hrs analysis was performed on the normalized values All values are the average of three immunoblots ± SEM Silencing of the...
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Báo cáo y học: "Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisia" pot
... through RRG10 (for 'Required for respiratory growth' ) as the functional analysis described below proves that their products are novel factors required for respiratory growth Comparative growth analysis ... affected genes are only indirectly related to maintenance of respiratory activity Genes required for protein translation in mitochondria Other factors required for mtDNA inheritance are Mgm1, Doc1 ... genes include, but are not limited to, genes encoding respiratory chain components, factors required for folding and assembly of respiratory chain subunits, proteins required for mitochondrial...
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báo cáo khoa học: " Plastid chaperonin proteins Cpn60α and Cpn60β are required for plastid division in Arabidopsis thaliana" docx
... and ptCpn60β are required for proper FtsZ ring formation To confirm the reduction of ptCpn60β proteins in ptcpn60β mutants and further examine localization of the proteins, we prepared antibodies ... indicating that FtsZ ring formation is perturbed in both mutants Discussion In this study, we showed that both ptCpn60α and ptCpn60β are required for plastid division as well as for greening of plastids ... FtsZ ring formation is impaired in the respective chaperonin mutants even though FtsZ protein levels are normal (Figure 4A) These results suggest that ptCpn60 proteins are not required for import...
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báo cáo khoa học: " Kinesin-5 motors are required for organization of spindle microtubules in Silvetia compressa zygotes" doc
... envelope breakdown (NEB) [14] when they are released into the cytoplasm and accumulate at the midzone and poles of the forming spindle They are required for maintenance of bipolar spindle integrity ... disruption slows tip growth Rhizoids of zygotes treated with paclitaxel, oryzalin or monastrol appeared shorter and broader than control rhizoids (Fig 5B–E) We therefore assayed tip growth by measuring ... Embryonic growth over a 72 h period Data are from one representative experiment sampling ten undivided zygotes for each treatment Diamonds, 0.2% DMSO; triangles, μM paclitaxel; squares, μM oryzalin;...
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Báo cáo y học: "Two discrete events, human T-cell leukemia virus type I Tax oncoprotein expression and a separate stress stimulus, are required for induction of apoptosis in T-cells" pptx
... enhances both cell growth (transformation) and cell death (apoptosis) ([34]) To dissect the cell growth/ death paradox relevant to HTLV-I, we sought to examine the requirements for Tax to cause ... transformed cell lines, as indicated, was after treatment with ZnCl2 or CdCl2 for 24 hours (B) or 48 hours (C) HTLV-I transformed cell lines treated with Zn showed lower cell viability compared ... emerges plausibly from our current work is that Tax primarily enforces changes in cellular metabolism for accelerated growth and transformation; however, these driving impulses may unwittingly dysregulate...
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Brutons tyrosine kinase and protein kinase c µ are required for TLR7 9 induced IKKα and IRF 1 activation and interferon β production in conventional dendritic cells
... centrifugation at 13,000 rpm for 10 mins at 4uC For immunoprecipitation studies, cell lysates were pre-cleared with Protein A/ G Plus-agarose in the presence of goat IgG (Santa Cruz) for h at 4uC mg of ... were added to the pre-cleared lysate and rotated for h at 4uC Subsequently, beads were washed times with phospholysis buffer and boiled in loading buffer for 10 mins For Western blot analyses, ... experiments and procedures were performed in compliance with Guidelines on the Care and Use of Animals for Scientific Purposes of the National Advisory Committee for Laboratory Animal Research (NACLAR)...
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Báo cáo khoa học: The b-N-acetylglucosaminidases NAG1 and NAG2 are essential for growth of Trichoderma atroviride on chitin doc
... NAG1 or NAG2 is essential for growth on chitin A B C D Fig Biomass and chitinolytic enzyme activities upon growth on chitin in shake flask cultures Values for biomass are given per mL of treated ... atroviride, our results clearly showed that they are essential for growth on chitin Dnag1Dnag2 strains showed significantly reduced biomass formation upon growth on colloidal chitin, and did not grow ... are not a natural growth medium for T atroviride, and whether the NAGases were cell-wallbound or extracellular did not influence growth under more natural conditions on agar plates, and therefore...
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Báo cáo khoa học: "The relative importance of cork harvesting and climate for stem radial growth of Quercus suber " doc
... and March to September (lighter bars) Average annual air temperatures are displayed for CJM (circles) and HP (squares) Stem growth of Quercus suber L 441 Table I Characteristics of the selected ... though rather poorly with growth variables The growth rate was high during the first two years (4.0–8.2 mm yr–1), as a new cork layer was being formed (figure 3) Afterwards, radial increments decreased ... “86”) On the first Spring after harvesting, radial increment was rather small at CJM (table II) as compared to the following years In contrast, at HP radial growth on the first Spring after harvesting...
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Báo cáo y học: "Wildtype epidermal growth factor receptor (Egfr) is not required for daily locomotor or masking behavior in mice" pps
... transforming growth factor-alpha and neuregulin-1 suppress active behaviors and cause weight loss in hamsters Brain Research 2005, 1038:171-182 Wakefield LM, Yang Y, Dukhanina O: Transforming growth ... demonstrated suppression of activity throughout the pulse Grey areas, lights off; white area, light pulse Page of (page number not for citation purposes) Journal of Circadian Rhythms 2006, 4:15 ... that EGFR activity is not required to produce and is not a major mediator of abnormal activity phenotypes, and that other environmental, genetic, or stochastic effects are required Table 1: Activity...
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AMINIMUM NUMBER OF PHYSICAL EDUCATION CREDITS SHOULD BE REQUIRED FOR GRADUATION OF COLLEGE
... each competitor that wins Thanks to it, we can learn to share, to be self sacrificed,to work together with others for common goal These are the reasons why I feel physical courses should be part ... rewards are distributed equally on the basis of mutual interactive behavior among individuals By this definition we learn that a cooperatives situation requires at least one competitor to fail for ... courses should be part of the graduation requirements of any university or college; they are of high value for a student’s health, character-training and his betterment , {468 words) ...
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Tài liệu In this lab, 2 ISDN routers are required. If ISDN routers are not available, review the lab to become familiar with the process. An Adtran Atlas550 ISDN emulator is used to simulate the switch/ISDN cloud. pdf
... the reason for the call This information is shown for each channel: Tokyo#show dialer h Which dialer strings are associated with Dialer1? _ i What is the last status for dial string ... DDR dialer information Configure the correct dialer information so that the dialer profile and dialer interface function correctly This includes all of the following: • IP address information • ... Step Specifying interesting traffic for DDR Traffic must be defined as ‘interesting’ to cause the DDR interface to dialup the remote router For the moment, declare that all IP traffic is interesting...
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why colors are important for brands
... spaces or animations to attract users Thus, all these are very simple tips to focus on and using them for small business can achieve unsurpassed growth rates It’s good to get things colored but should ... matter a lot Connect: Aut hored by: Samir Feroze Samir Feroze is the founder of VeriQual Ltd, a software services company specialising in RoR, PHP, Android and iOS He has also founded Yello.PK which ... technology, Location based applications, financing early stage ventures and outsourcing In his spare time he likes to work on rotary See complet e prof ile Would you like to contribute to this...
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Tài liệu T24 – Giải pháp ngân hàng trung tâm thế hệ mới đối với phát triển bền vững - New Generation Core Banking System For Sustained Growth pdf
... information • Improving Productivity – All modules are integrated – Front, middle and back office operations are fully supported – Timely information are provided Thank you! ... book • Multi-server Architecture • Non-stop Processing • Improved Performance Enabling Growth • AZ T24 Upgrade - Benefits derived for Techcombank • Increased ROIs – Develop new products and services ... take care of the rest • Reducing Costs – Improved efficiency in real-time processing results in lower costs – Manual processes and paper flows are reduced by increasing computerized information...
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Tài liệu effective internet presenceted demopoulos’now required for success in business and life doc
... They are all online Who Are You? Who are you? Who, who, who, who? I really wanna know Who are you? Who, who, who, who? Tell me, who are you? – The Who, 1978 Today when people want to know “Who are ... Internet Presence: Now required for success in business and life Ted Demopoulos There are lots of choices, and there are bound to be some you enjoy and that work well for you Develop an action ... • Hobbies • Family • Foolishness Of course these are just guidelines Some of them are open to debate Effective Internet Presence: Now required for success in business and life Ted Demopoulos Notice...
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