network drivers part 2 packet reception and transmission
![progress in botany. 73 [electronic resource]](https://media.store123doc.com/images/document/14/y/md/small_mdt1401382716.jpg)
progress in botany. 73 [electronic resource]
... E2 S2 k24f, k24r 25 E1 S2 þ E2 S3 ! E1 S2 E2 S3 k25f, k25r 26 E1 S1 E2 S2 ! E1 S2 E2 S2 k26f, k26r 27 E1 S1 E2 S2 ! E1 S2 E2 S3 k27f, k27r 28 E1 S2 E2 S2 ! E1 S2 E2 S3 k28f, k28r 29 E1 S1 E2 ... 20 E1 E2 S3 þ S1 ! E1 S1 E2 S3 k20f, k20r 21 E1 E2 S3 þS2 ! E1 S2 E2 S3 k21f, k21r 22 E1 S1 þ E2 S2 ! E1 S1 E2 S2 k22f, k22r 23 E1 S1 þ E2 S3 ! E1 S1 E2 S3 k23f, k23r 24 E1 S2 þ E2 S2 ! E1 S2 ... k1r E1 þ S2 ! E1 S2 k2f, k2r E2 þ S2 ! E2 S2 k3f, k3r E2 þ S3 ! E2 S3 k4f, k4r E1 S1 þE2 ! E1 S1 E2 k5f, k5r E1 S2 þE2 ! E1 S2 E2 k6f, k6r E2 S2 þE1 ! E1 E2 S2 k7f, k7r E2 S3 þE1 ! E1 E2 S3 k8f,...
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Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling doc
... Dreze M, Ayivi-Guedehoussou N et al (20 05) Towards a 19 92 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 proteome-scale map of the human protein–protein interaction network Nature 437, 1173–1178 Paravicini ... 99 100 101 1 02 receptor coactivator 1-dependent coactivation of transcription Mol Endocrinol 20 , 21 22 21 40 Kim MJ, Kim HR & Paek KH (20 06) Arabidopsis tonoplast proteins TIP1 and TIP2 interact ... Spindler-Barth M (20 02) Ligand-induced heterodimerization between the ligand binding domains of the Drosophila ecdysteroid receptor and ultraspiracle Eur J Biochem 26 9, 323 7– 324 5 Dues G, Muller...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... Baird CL (20 09) Engineering an ultra-stable affinity reagent based on Top7 Protein Eng Des Sel 22 , 325 –3 32 FEBS Journal 27 7 (20 10) 20 06 20 14 ª 20 10 The Authors Journal compilation ª 20 10 FEBS ... Biotechnol 23 , 125 7– 126 8 59 Hosse RJ, Rothe A & Power BE (20 06) A new generation of protein display scaffolds for molecular recognition Protein Sci 15, 14 27 FEBS Journal 27 7 (20 10) 20 06 20 14 ª 20 10 ... 27 7, 19 82 1995 Tomizaki K, Usui K & Mihara H (20 10) Protein– protein interactions and selection: array-based techniques for screening disease-associated biomarkers 20 12 15 16 17 18 19 20 21 22 ...
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Báo cáo y học: "Natural selection of protein structural and functional properties: a single nucleotide polymorphism perspect" potx
... http://genomebiology.com /20 08/9/4/R69 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Genome Biology 20 08, Sirotkin K: dbSNP: the NCBI database of genetic variation Nucleic Acids Res 20 01, 29 :308-311 Jiang ... Two−hybrid interactions 0 .2 0.1 0 2- 4 5-10 All interactions 2. 8 Two−hybrid interactions 2. 6 2. 4 2. 2 >10 Number of interaction partners 2- 4 5-10 >10 Number of interaction partners Figure Selective ... proteins, [0 .22 , 0 .26 ] for cytoplasmic proteins, and [0 .26 , 0 .29 ] for membrane proteins transporter activity' and 'motor activity', and the opposite groups are 'receptor activity' and 'isomerase...
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Development of a novel toll like receptor based two hybrid assay for detecting protein protein interactions and its application in the study of CD14 dimerization and FcyRIIA activation
... digestion 72 2.1 .2. 17 Site-directed mutagenesis 73 2. 1 .2. 18 Sequencing 74 2. 2 Cell biology 75 2. 2.1 Materials 75 2. 2.1.1 Stimulant 75 2. 2 .2 Methods 75 2. 2 .2. 1 Mammalian cell culture 75 2. 2 .2. 2 Storage ... 76 2. 2 .2. 3 Liposome-based cell transfection 76 2. 2 .2. 4 Using calcium phosphate cell transfection 77 2. 2 .2. 5 Dual luciferase assay 77 2. 2 .2. 6 Treatment of cells with specific stimuli 78 2. 2 .2. 7 ... 2. 2 .2. 8 Isolation of human peripheral blood monocytes 80 2. 2 .2. 9 Generation of macrophages 81 2. 2 .2. 10 Cell activation 81 2. 2 .2. 11 Preparation of ImIgG, Heat aggregated-IgG and IgG beads 81 2. 2 .2. 12...
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Báo cáo khoa học: On peptide bond formation, translocation, nascent protein progression and the regulatory properties of ribosomes ppt
... A- and P-loops, 25 56–7 and 22 54–5, respectively, can be superimposed on each other with no apparent deviations, whereas noticeable differences are found between their neighbors, 25 54–5 and 22 52 3 ... A-site tRNA and G2553 [41], can be formed in the symmetryrelated region While rotating, the RM interacts with the rear-wall bases belonging to nucleotides C2573, A2451, and C24 52, and slides along ... of L 22; on the conservation of the L 22 double-hook; and on the finding that sequence related translational arrest could be suppressed by mutations localized in the L 22 double-hook region [ 72] We...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... 20 09 FEBS K R Wolthers and N S Scrutton 19 20 21 22 23 24 25 26 27 28 stopped-flow spectrophotometry Biochemistry 43, 490– 500 Wolthers KR & Scrutton NS (20 07) Protein interactions in the human methionine ... 0.01 ± 0.01 0. 02 ± 0.01 0. 02 ± 0.01 0.13 0.91 0.75 1. 12 0.09 0.18 ± ± ± ± ± ± 0.01 0.14 0. 02 0.04 0. 02 0. 02 0.06 1 .23 1 .20 1.19 0 .20 0.19 ± ± ± ± ± ± 0.01 0.15 0.10 0.03 0.01 0. 02 stimulation ... n-fold 8500 1880 0.3 4100 21 00 420 0.5 1.1 1540 100 50 10 2. 3 3669 FEBS Journal 27 6 (20 09) 19 42 1951 ª 20 09 The Authors Journal compilation ª 20 09 FEBS K R Wolthers and N S Scrutton nine from...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... (LSHM-CT -20 06-036903, MTKD-CT -20 06-0 425 86 and COST Action BM06 02) and by the Polish Ministry of Scientific Research and Information Technology (2 P05A01 127 , 63 ⁄ 6PRUE ⁄ 20 07 ⁄ and 339 ⁄ 6PR-UE ⁄ 20 07 ... IL-1beta MCPIP protein as an RNase 19 20 21 22 23 24 25 26 27 28 mRNA show unequal IL-1beta protein accumulation but similar polyribosome profile J Immunol 153, 27 7– 28 6 Dinarello C (1996) Biologic ... residues (D141, E185, D 226 and D244) involved in binding Mg2+ ions during RNA degradation 7388 FEBS Journal 27 6 (20 09) 7386–7399 ª 20 09 The Authors Journal compilation ª 20 09 FEBS Cytoskeleton...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... Gomez (Eur J Biochem 27 1) 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 binding domain: a model system for analysis of solute eects on protein processes Biochemistry 42, 22 022 217 Courtenay, E.S., ... )40 )29 )23 170 144 129 131 96 77 )22 8 )171 )153 )151 )106 )81 28 5 28 8 29 1 29 0 29 4 29 6 c 0.3 0.1 0.3 0.5 2 3 2 2 3 6 10 10 10 9 DCp (kcalặmol)1ặK)1) Ts (K) 3 3 28 9 ... 333.3 3 32. 6 327 .6 322 .2 313 Tm  (K) Tm (K) DHm (kcalặmol)1) DHmc (kcalặmol)1) DSm (calặmol-1ặK-1) DSmc (calặmol)1ặK)1) Th (K) 25 6 26 2 26 6 26 7 27 5 28 2 58 48 43 43 31 24 )58 )45...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... PEP CO2 CO2 Oxalacetate Oxalate Oxalate Metal ion Mn2+ Mg2+ Mn2+ Mn2+ Mn2+ Mn2+ Mg2+ d Mn2+ Mn2+ Mn2+ Mg2+ d a Kd (lM) 17.4 1400 1800 24 33.8 24 .8 128 508 0 .24 53.8 10 300 13 700 156 26 136 ... presence of substrates and metal ions at saturating concentrations Substrate or ligand s1 (ns) f1 s2 (ns) f2 v2 ATP, Mn2+ PEP, Mg2+ PEP, Mn2+ ATP, oxalate, Mg2+ 5.15 5 .27 5. 52 6.10 5.75 0.34 0.37 ... dierent ligands at saturating concentrations The error is estimated as 5% of stated values Ligand kq (109 ATP, Mn2+ ATP, Mg2+ ATP, oxalate, Mg2+ ADP, Mn2+ AMP, Mn2+ Oxaloacetate, Mn2+ CO2, Mn2+ PEP,...
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... Acad Sci USA 91, 22 60– 22 64 34 Welcker M, Singer J, Loeb KR, Grim J & Bloecher A (20 03) Multisite phosphorylation by Cdk2 and GSK3 controls cyclin E degradation Mol Cell 12, 381–3 92 35 Kim SY, Song ... signaling network Science 29 7, 1018–1 023 24 Heinrich R, Neel BG & Rapoport TA (20 02) Mathematical models of protein kinase signal transduction Mol Cell 9, 957–970 25 Roach PJ (1991) Multisite and hierarchal ... Hill coefficient ranges between and (Fig 3E) For random and mixed FEBS Journal 27 4 (20 07) 1046–1061 ª 20 07 The Authors Journal compilation ª 20 07 FEBS C Salazar and T Hofer ¨ Kinetic models of...
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Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot
... Biophys Acta 1387, 22 6 23 8 30 Ryan, M., Carlson, B.M & Ohlendieck, K (20 00) Oligomeric status of the dihydropyridine receptor in aged skeletal muscle Mol Cell Biol Res Commun 4, 22 4 22 9 31 He, Z., ... SDS/PAGE under reducing conditions was carried out by standard methodology [21 ] using 7% gels and 20 lg protein per lane [22 ] Protein band patterns were visualized by Coomassie Brilliant Blue ... calcium-handling proteins in native sarcoplasmic reticulum membranes from rabbit skeletal muscle Biochim Biophys Acta 1515, 120 –1 32 Ó FEBS 20 02 Supramolecular calsequestrin complex (Eur J Biochem 26 9)...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... Journal 27 7 (20 10) 1597–1605 ª 20 10 The Authors Journal compilation ª 20 10 FEBS Biophysical properties of hGBP5a ⁄ b and hGBP5ta M Wehner and C Herrmann to results obtained for hGBP1 and hGBP2 [17], ... 2. 6 ± 0.3 15 ± )14.7 ± 0.1 )31.3 ± 0.4 )21 .4 ± 0.8 )7.7 ± 0 .2 )23 .5 ± 0.6 )14.9 ± 0.9 11 ± ± 0 .2 26 ± )16.5 ± 0.1 )25 .3 ± 0.3 )38.1 ± 0.8 )9.7 ± 0 .2 )18 .2 ± 0.3 )31.9 ± 0.9 exothermic effects ... mant-GDP 0.046 ± 0.003 0 .28 ± 0.04 0 .23 ± 0. 02 5.0 ± 0.8 mant-GTPcS 0.099 ± 0.003 2. 08 ± 0.04 1.11 ± 0.01a 11 .2 ± 0.5 0.19 ± 0.02b 1.9 ± 0.3 mant-GTP 0.014 ± 0.001 0. 62 ± 0.01 – 44 ± 4* hGBP5ta...
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Báo cáo Y học: Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein–protein interactions pdf
... 13 and 16) Truncations of IRF-3 to amino acids 328 , 26 4 or 24 1 resulted in much stronger binding to the ISRE (lanes 19, 22 and 25 ) In the presence of GST-CBP-N a doublet of supershifted bands ... Wathelet, M.G (1998) Structure and Function of Ó FEBS 20 02 10 11 12 13 14 15 16 17 18 19 20 21 22 Mechanism of IRF-3 virus-dependent activation (Eur J Biochem 26 9) 6151 the Interferon-b Enhanceosome ... -p300-N, -M and -C were described previously [24 ], GST-CBP-N1, -N2, -N3, -C1, -C2, -C3 and GST-p300-C1, -C2, -C3 were generated by subcloning PCR products and verified by sequencing GST and GST fusions...
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Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt
... Biol Chem 26 0, 788–794 22 MacLennan, D.H., Rice, W.J & Green, N.M (1997) The mechanism of Ca2+ transport by sarco (endo) plasmic reticulum Ca2+-ATPases J Biol Chem 27 2, 28 815 28 818 23 Michalak, ... 12 in vivo and by casein kinase I J Biol Chem 26 8, 26 7 12 26 718 36 Cowles, E.A., Agrwal, N., Anderson, R.L & Wang, J.L (1990) Carbohydrate-binding protein 35 Isoelectric points of the poly- 3 820 ... changes in its distribution during end plate development and after denervation Proc Natl Acad Sci USA 86, 721 8– 722 2 29 Keryer, G., Skalhegg, B.S., Landmark, B.F., Hansson, V., Jahnsen, T & Tasken, K...
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Báo cáo khoa học: Calcium-dependent protein–protein interactions induce changes in proximity relationships of Cys48 and Cys64 in chicken skeletal troponin I ppt
... sTnC and cTnC into TnT-treated myofibrils was compared by running the SDS/PAGE Densitometric pCa ± ± ± ± ± ± 3.83 17.63 5 .23 3 .23 2. 22 2.86 28 6.37 110. 42 303.83 27 7 .25 301.18 1 02. 35 ± ± ± ± ± ± 22 .0 ... muscle FEBS Lett 70, 25 4 25 6 25 Reinach, F.C & Karlsson, R (1988) Cloning expression, and sitedirected mutagenesis of chicken skeletal muscle troponin C J Biol Chem 26 3, 23 71 23 76 26 Kobayashi, T., ... FEBS Lett 181, 28 1 28 5 29 Liou, Y.M & Fuchs, F (19 92) Pyrene-labeled cardiac troponin C: Effects of Ca2+ on monomer and excimer fluorescence in solution and myofibrils Biophys J 61, 8 92 901 30 Liou,...
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Báo cáo khoa học: Structural and mutational analyses of protein–protein interactions between transthyretin and retinol-binding protein doc
... 65.5–1.59 (1.63–1.59) 3 027 6 (20 71) 3 .2 (2. 0) 99.1 ( 92. 9) 17.3 (2. 1) 0.051 (0. 423 ) P2 121 2, a = 42. 56 b = 86 .28 , c = 64.83 64.8 2. 30 (2. 42 2. 30) 9936 (1599) 5.9 (6 .2) 99.3 (100) 5 .2 (3.9) 0.095 (0.156) ... Rmergea a V20S TTR variant Y114H TTR variant C 222 , a = 159. 62, b = 22 3.18, c = 121 .43 15. 72 3.36 (3.51–3.36) 25 746 (1 622 ) 3.0 (2. 6) 84.4 (77.7) 3.3 (1.9) 0.16 (0.34) P2 121 2, a = 42. 00 b = 83.81, ... 94 0 .23 9 (0.313) 0.3 12 (0.357) 37.5 13.4 0 .20 9 (0 .27 7) 0 .22 9 (0.303) 17.8 – 0 .21 8 (0 .25 8) 0 .28 1 (0. 326 ) 23 .3 – 0.010 1.5 0.011 1 .29 0.006 1.3 jj Rcryst ¼ Rhkl RFo jÀkjjFc jj, where |Fo| and |Fc|...
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dna–protein interactions principles and protocols second edition - tom moss
... M., and Draper, D E (1991) Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostrepton J Mol Biol 22 1, 125 7 126 8 Wyman, J and ... Curtis A Machida, 20 00 125 Glycoprotein Methods and Protocols: The Mucins, edited by Anthony P Corfield, 20 00 124 Protein Kinase Protocols, edited by Alastair D Reith, 20 01 123 In Situ Hybridization ... Hybridization Protocols (2nd ed.), edited by Ian A Darby, 20 00 122 Confocal Microscopy Methods and Protocols, edited by Stephen W Paddock, 1999 121 Natural Killer Cell Protocols: Cellular and Molecular...
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Báo cáo hóa học: " Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions" pdf
... Biochem Biophys 20 06, 453:108- 122 Page 10 of 11 (page number not for citation purposes) Virology Journal 20 08, 5:61 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 Shepherd DN, ... PstI and partially digested with NcoI Fragments of both plasmids approximately 0.33 kbp, 0.69 kbp, and 4.4 kbp in size (respectively referred to as K1, K2, and K3 for pKNco and S1, S2, and S3 ... purified fragments from pKNco and pSNco, using the following combinations of fragments: 1) S1, K2, K3; 2) K1, S2, K3; 3) S1, S2, K3; 4) K1, S2, S3; 5) S1, K2, S3; 6) K1, K2, S3 These six ligations...
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