... shunfriends and family and apprehended going to the grocery store whereshe dreaded the supermarket owner’s greetings and offers of help.Her husband’s business activities included a certain amount of ... of the hands and a mask-like rigidity of the face.4. Sweating À through evaporation it cools off straining muscles.5. An urge to urinate (in some an inability to do it). Intestinal cramps and ... similarities and unique responses to culturally defined socialdemands. Chapter 2 traces the historic evolution of the notion of socialphobia and its equivalents (mostly from the end of nineteenth...
... permission.Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.The Natureand Scope of MarketingHunt, Shelby D.Journal of Marketing (pre-1986); Jul ... without permission.Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.Reproduced with permission of the copyright owner. Further reproduction ... Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.Reproduced with permission of the copyright owner. Further reproduction...
... Identi®cation of the sipV(ba) and sipW(ba) gene regions of B. amyloliquefaciens. (A1/B1) PCR reactions with genomic DNA of B. amy-loliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and ... PstIandEcoRI in case of sipV or EcoRI-SacIincaseofsipW and ligated into corresponding sites of pUC18 DNA. The ligation mixes were used for PCR with oligo nucleotides Uni1 or Uni2 and pairs of ... 2002Identi®cation andpropertiesof type I-signal peptidases of Bacillus amyloliquefaciensHoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer and JuÈ rgen HofemeisterInstitute...
... theKm and kcatvalues of E. coli Fre for riboflavin arereported as 2.5 lm and 52.4 s)1with NADPH as theelectron donor and 1.3 lm and 30.6 s)1with NADH[13]. For FMN, Km and kcatvalues of ... twofold axis associated with thedimer passes through the vicinity of the side chains of Pro13, Ile126, Gln133 and Ile163. The correspondingTable 1. Summary of x-ray data collection, phasing and ... the case of A. vinelandii ferric reductase, the enzymewas purified as a mixture of two proteins or a hetero-dimer with molecular masses of 44 600 and 69 000 Da[25]. Although none of the amino...
... decomposition of tetra-thionate was proposed by Steudel et al.[16],Hazeuet al.[17] and Meulenberg et al. [18] in experiments with intactcells of A. ferrooxidans and A. acidophilum.Propertiesofpurified ... mMNADP+(Sigma) and enzyme. The production of NADPH was determinedspectrophotometrically at 340 nm. One unit of Glc6Pdehydrogenase was defined as the amount of protein thatreduced 1 lmol of NADP+in ... enzyme may bedue to several reasons. Copper and several other divalentions stimulated the activity of the tetrathionate hydrolase and they and other cofactors may be present in the crudeenzyme...
... lombricine (30% of that of taurocyam-ine) and glycocyamine (7% of that of taurocyamine). Neither TK catalyzedthe phosphorylation of creatine. Comparison of the deduced amino acidsequences of mitochondrial ... 3381–3385.Cytoplasmic and mitochondrial taurocyamine kinases K. Uda et al.3530 FEBS Journal 272 (2005) 3521–3530 ª 2005 FEBSOrigin andpropertiesof cytoplasmic and mitochondrialisoforms of taurocyamine ... correlations of this relaxed sub-strate specificity and the possibility of cytoplasmic and mitochondrial isoforms of TK, we have amplified twocDNAs coding for Arenicola brasiliensis TKs and cloned...
... mixture of 0.05MNaPibuffer, pH 3, and acetonitrile (6 : 1, v/v);separation was performed isocratically, at a flow rate of 1mLÆmin)1, and the volume of injected samples was 10 lL.The amounts of ... & Grisebach, H. (1982) Enzymic synthesis of lignin precursors. Purification andpropertiesof 4-coumarate:CoA ligase from cambial sap of spruce (Picea abies L.). Eur. J.Biochem. 123, 583–586.12. ... the method of Baayen and Elgersma [13], with a 500-lLdropofFod conidialsuspension at a concentration of 9 · 106conidiaÆmL)1;20additional plants, inoculated with a 500-lL drop of doubledistilled...
... lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an[a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rabproteins, ... GST±Rab31 and Fig. 7. Puri®cation andproperties o f wild-type and mutant Rab31 and Rab32 expressed a s GST±fusion proteins. Samples of platelet particu-late fraction protein andof puri®ed GST and ... expression andpropertiesof Rab31 and Rab32New blood platelet Rab proteinsXiankun Bao1, Andrea E. Faris2, Elliott K. Jang1 and Richard J. Haslam1,2Departments of 1Pathology and Molecular...
... The effect of geometrical parameters and material propertiesof facesheets and core are studied on the face wrinkling of sandwich plates. Comparison of the present results with those of plate ... anisotropic propertiesof strength and stiffness of masonry in horizontal and vertical direction, tensile and shear stiffness of cracked masonry,• Concrete yielding in compression, opening of cracks ... tension,mechanism of opening and closing of cracks in concrete under dynamic load,tensile and shear stiffness of cracked concrete,• Strain rate effect of the material propertiesof masonry, reinforced...
... expression andpropertiesof the recombinant enzymeY. T. Pan1, J. D. Carroll2 and A. D. Elbein11Department of Biochemistry and Molecular Biology and 2Department of Microbiology and Immunology, ... the cloning and expression of the M. tuberculosis tps gene in E.coli,andtheproduction of active TPS in these cells. The propertiesof therecombinant enzyme have been determined, and are com-pared ... starting at position 397 of the M. tuber-culosis TPS, and another sequence of about 16 amino acidsstarting at position 421 of the M. tuberculosis TPS.Isolation and purification of recombinant TPSTo...
... the effect of a mutation in this part of the molecule on the struc-ture andpropertiesof human sHsp.Oligomeric structure of HSP22 and its mutantsAll samples of recombinant HSP22 and its mutantspurified ... understand the role of this part of molecule in the structure and func-tion of small heat shock proteins, we mutated two highly conservative resi-dues (K137 and K141) of human HSP22 and investigated ... trypsinolysis of the wild-type HSP22 and its K137E and K137,141E mutants. Kinetics of trypsinolysis of the wild-typeHSP22 (A) and its K137E (B) and K137,141E (C) mutants. The time of incubation...
... Carroll4,Irena Pastuszak1 and Alan D. Elbein11Department of Biochemistry and Molecular Biology and 4Department of Microbiology and Immunology, University of Arkansas forMedical Sciences, ... a number of individualpapers can be s treaked with the sugar solution and all run atthe same time in the same solvent. Standards of trehalose and maltose are applied to the sides of the paper ... and 20 mMconcentration of the unlabeledother disaccharide (cold mMmaltose with radioactive trehalose and vice versa). The amount of radioactive m altose p roduced from treha-lose, or vice...