nano micro patterning of semiconductors by site selective chemical etching using noble metals as catalyst
Báo cáo khoa học: Probing suggested catalytic domains of glycosyltransferases by site-directed mutagenesis Tobias Hefner and Joachim Stockigt ¨ ppt
... SDS/PAGE and staining by Coomassie-blue (I, marker proteins; II, AS- WT; III, AS- L86I; IV, AS- A204V; V, AS- E368D; VI, AS- E368A; VII, AS- H360K; VIII, AS- H360R; IX, H360E; w, arbutin synthase and its muteins) ... model For this model it has been assumed that mutation of His by other basic amino acids such as Arg or Lys would probably be tolerated by enzymes of the NRD1 family In contrast to this theory, the ... synthase exhibits the His-Glu site (Fig 2), proposed as a catalytic domain of general importance for the mechanism of sugar transfer The significance of this HisGlu site we also explored by an...
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Báo cáo hóa học: " Properties of nanocones formed on a surface of semiconductors by laser radiation: quantum confinement effect of electrons, phonons, and excitons" pptx
... Appearance of nanocones on the irradiated surface of semiconductors and their height dependence to the laser intensity has been found by measurements of the irradiated surface morphology by AFM, as shown ... irradiated surface of Si0.7Ge0.3 [18], as shown in Figure 4 Non-monotonous dependence of Si crystal microhardness as a function of the laser intensity The increase of microhardness with increasing LR intensity ... The decrease of the microhardness is explained by formation of nanocones as a result of plastic deformation of the mechanically stressed layer, which is characteristic to the SLA stage, as shown...
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Báo cáo hóa học: " Fabrication of ordered nanoporous anodic alumina prepatterned by mold-assisted chemical etching" docx
... mechanism of localized microetching relied on the diffusive transport of chemicals within a stamp [18-20] Figure shows the scheme of mold-assisted microetching of substrate The PDMS stamp was soaked ... mediated by a PDMS template By means of using the diluted (2%) mixed acid solution as a chemical etchant, the wet soft stamp can indent nanoscale shallow concaves on aluminum without the need of excessive ... material by simply contacting with the substrate (e.g., HF for SiO2 or HCl/FeCl for Cu) [18-20] Localized etching is mediated by a mold-assisted chemical etching initiated from the stamp microfeatures,...
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Báo cáo khoa học: Highly site-selective stability increases by glycosylation of dihydrofolate reductase ppt
... provide further support [14] that, at least in the case of dihydrofolate reductase, increased stability through the addition of glycans is because of highly site- specific effects rather than nonspecific ... thermal stability of site- selectively glycosylated dihydrofolate reductase ChemBioChem 6, 1338–1340 15 Baek WO & Vijayalakshmi MA (1997) Effect of chemical glycosylation of Rnase A on the protein ... generation of neoglycoproteins via site- selective glycosylation of proteins using chemical modification of biotechnologically produced proteins [2,3,28–31] One such approach combines site- directed...
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Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site specificities and release of matrikines pptx
... MALDI-LIFT-TOF ⁄ TOF MS ⁄ MS experiments by applying 2000 laser shots Proteolysis of tropoelastin For proteolysis using the catalytic domains of either MMP-7 or MMP-12, recombinant tropoelastin was dissolved ... ratio of : 500 (m ⁄ m) for h at 37 °C Before MS analysis, all samples were stored at )30 °C MALDI-TOF ⁄ TOF MS analysis Analysis of the digests was conducted by of ine nanoHPLC ⁄ MALDI-TOF ⁄ TOF ... are based on the total amounts of each of the amino acids in tropoelastin (isoform 2) (2 of 157 possible x-Ala bonds) but are cleaved to a small extent by MMP-9 (12 of 157) and even more by MMP-12...
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Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
... Fig Schematic view of the active site of ACE2 and tACE Binding interactions of the inhibitor (A) MLN-4760 at the active site of ACE2 and (B) lisinopril at the active site of tACE Hydrogen bonds ... the CL2 site may be absent Conclusion This study has highlighted the importance of Arg273 in binding of the C-terminus of peptide substrates by ACE2 The complete loss of activity observed as a result ... ACE makes sense in view of the fact that ACE2 has only one chloride-binding site (CL1) whereas ACE has two sites (one in each subdomain) In addition to this, the CL1 site is found in subdomain...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... properties of the wild-type, His120Asn, His145Asn and Asn149Asp variants of arginase II Purified wild-type, His120Asn and His145Asn variants of arginase II were active even in the absence of added ... agmatine was only about 5% of the arginase activity of the wildtype enzyme As measured by kcat ⁄ Km, the catalytic efficiency of the Asn149Asp variant was found to be about 36-fold lower than that of ... agmatinase, the Asn149Asp was markedly resistant to inhibition by arginine (Fig 4) Clearly, arginine was very poorly recognized as a substrate or inhibitor by the Asn149Asp variant The opposite...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... structures by using the software package provided with the instrument Molecular modelling The agmatinase structural model was obtained by homology methods, using the structure of B caldovelox arginase ... of the structures of B caldovelox arginase (red), rat liver arginase (yellow) and the modelled structure of E coli agmatinase (blue) Note the shorter extension, in the case of E coli agmatinase, ... replacement of Asp153 with asparagine, the whole topology of the active site was found to be conserved in agmatinase In the modelled active site structure, the side chains of Asp153 and Asn153 can be accommodated...
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Tài liệu Báo cáo Y học: Study of substrate specificity of human aromatase by site directed mutagenesis pdf
... quantification of the aromatase expressed was used as an indicator of the transfection efficiency Whole cell aromatase activity and inhibition Aromatase activity was assessed in whole cells using the ... by human P450arom cDNA and the aromatase activity was evaluated by the tritiated water assay using [1b,2b-3H]-androstenedione as a substrate, as described in Materials and methods The aromatase ... aromatase using testosterone or nor-testosterone as substrates Cells were transfected by human P450arom cDNA and the aromatase activity was evaluated by radioimmunoassay of 17b-estradiol, using testosterone...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... representation of the relative densities of the free DNA measured at increasing concentrations of HbpR The percentage of free DNA was calculated from densitometric measurements of the radiolabeled bands as ... sequence, or different mutants (as indicated at the top of each panel), in the presence of increasing concentrations of HbpR fusion protein (0–1200 nM) Assignment of the UAS mutant names corresponds ... in DNaseI footprints by HbpR [19] and are used as such also in other figures Sequence numbers refer to the locations of the transcriptional start site of hbpC (B) Alignments of the sequences of...
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Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot
... Cys proteases, the basic pKa has been attributed to the triad His [26,32] Elucidation of the inuence of His107 and Asp122 on the catalytic reactivity of Cys68 has remained elusive, as The catalytic ... protein assay [53] NAT2 activity assay The specic activity of wild-type and mutant NAT2 was measured using PNPA as the acetyl donor and PABA as the acetyl acceptor in Mops buffer (pH 7, 25 C), as ... nm of the formation of Schiff base (e450nm = 52835 m)1ặcm)1) [54] The initial velocity of the reaction with AcCoA PNA was measured as a linear decrease in the absorbance at 430 nm because of...
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Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... (AT1) appears as two fragments corresponding to alternative sites of proteolysis; one fragment has a molecular mass of 32582 Da and the second has a molecular mass of 32739 Da As expected, after ... regulated by the rate of release of the diketide product by the TE domain Studies of the extent of loading of multienzymes with more than one module are needed to establish if product release is ... in a PKS has been seen when the levels of a PKS were increased without also increasing the levels of intracellular precursors [15] The throttling back of the process of product release could...
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Báo cáo khoa học: Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis potx
... increased GTPase activity in the case of the GST–S4 mutant (130.7% ± 3.4% as compared with GST–WT as 100%), and greatly increased GTPase activity in the case of the GST–S5 mutant (353 ± 38% as ... case of the filter paper method in the presence of mM Ca2+ (D) Inhibition of residual transglutaminase activity of recombinant TG2s by GTP, using the microtiter plate method Activity is shown as ... that might serve as Ca2+-binding sites [19] The aim of the present study was to identify the exact Ca2+-binding sites of human TG2, by using sitedirected mutagenesis and targeting sites homologous...
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Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... (A) Cleavage of angiotensin I by tACE (B) Cleavage of angiotensin I by ACE2 (C) Cleavage of angiotensin II by ACE2 Generation of product was determined by HPLC Values are the mean of triplicate ... (A) Cleavage of angiotensin I by tACE variants (B) Cleavage of angiotensin I by ACE2 variants (C) Cleavage of angiotensin II by ACE2 variants Generation of product was determined by HPLC Numbers ... site- mediated regulation of tACE and ACE2 activities are unknown In addition, although Arg1098 has been identified as essential to chloride regulation of the CL2 6034 site of sACE, the roles of...
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Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx
... nm, determined as described by Thannhauser et al [22], was used For activity measurements, the concentration of the RNase stock solutions was determined by use of the BCA protein assay kit (Pierce, ... Heidelberg, Germany) The rate constants of proteolysis (kp) were calculated from the decrease in the peak areas of the intact RNase band as a function of time of proteolysis, which followed pseudo-rst-order ... Results Design of the RNase A variants Analysis by use of the program FIRST [30] (http:// rstweb.asu.edu/) indicates the highest exibility of the peptide backbone of native RNase A at the N-terminus...
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Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot
... KARI was added to a final concentration of mgÆmL)1 and the absorbance at 412 nm was followed The data were analyzed as described in Results NADPH binding was measured as described by Dumas et ... activity was readily measured and could be used for comparing the isomerase activity of mutants with that of the wildtype The equilibrium constant for the isomerase reaction was estimated by incubating ... at a single active site [1] One of the main purposes of this study was to try to dissect the two reactions by mutagenesis of active site residues, expecting to find mutants of the E coli enzyme...
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Cancer incidence, mortality and survival by site for 14 regions of the world. pdf
... of using available incidence distributions by site, together with estimates of site- specific survival, to estimate the distribution of cancer deaths by site Table Globocan 2000 estimates of global ... Cancer was estimated to account for about million deaths (12% of all deaths) worldwide in 2000 (1), only preceded by cardiovascular diseases (30 % of all deaths), and by infectious and parasitic ... numbers of cases for the year 2000 These data have been used to estimate the global burden of cancer as part of the Global Burden of Disease 2000 project (GD 2000) (12) Version estimates of cancer...
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Báo cáo khoa học: Mapping of the active site of glutamate carboxypeptidase II by site-directed mutagenesis potx
... JH (2004) Synthesis of ureabased inhibitors as active site probes of glutamate Active site of glutamate carboxypeptidase II 35 36 37 38 39 40 carboxypeptidase II: efficacy as analgesic agents ... al Active site of glutamate carboxypeptidase II dent on the nature of the amino acid newly introduced When Asn519 was mutated to aspartate, the N519D mutant exhibited a 24-fold increase in Km ... determined by a radioenzymatic assay 4736 increased by two- to five orders of magnitude The highest increase was observed for the R210A mutant protein, which showed a KI value five orders of magnitude...
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Báo cáo khóa học: Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide doc
... SSU fused to GFP As above As above Transit peptide of cysteinyl-tRNA synthetase fused to GFP As above As above Transit peptide of histidyl-tRNA synthetase fused to GFP As above As above None pTSSU.tp.S34A-GFP ... fluorescence was determined by confocal microscopy as described [19] Results Generation of fusion constructs and mutagenesis of the phosphorylation signal The consensus phosphorylation site in chloroplast ... Alteration of the phosphorylation site did not impede targeting of the passenger GFP to the chloroplasts, nor was there any mistargeting to mitochondria The pattern of GFP fluorescence after targeting by...
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Báo cáo khoa học: Defining the QP-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy doc
... region of FrdB on the cytoplasmic side of the membrane The other site, the ˚ QD site (the distal Q -site) is located approximately 25 A from the QP site on the opposite (periplasmic) side of the ... sites towards the inner (cytoplasmic) and outer (periplasmic) sides of the membrane-intrinsic domain of the enzyme (FrdCD) One site, the QP site (the proximal Q -site) , is located in the interface ... there may be two Q-sites present – a polar QB site (equivalent to the QP site) , and an apolar QA site (equivalent to the QD site) [13–15] Investigation of the steadystate kinetics of quinol-dependent...
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