một số quy định pháp luật liên quan đến công tác bảo vệ
Báo cáo y học: " TGF-β1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by " pot
... transcription and quantitative realtime PCR Total RNA was extracted as previously described [25] The ABI 7300 real-time PCR machine (Applied Biosystems, Foster City, CA) was used for real-time quantitative ... 3.1.7 image acquisition software lated with TGFβ1 ng/ml and E-cadherin and αSMA expression quantified by quantitative real time PCR TGFβ1 significantly reduced E-cadherin mRNA levels while simultaneously ... isolated and assessed in triplicate for the expression of E-cadherin, α SMA and β-actin by means of quantitative real-time PCR Expression levels were normalized to the housekeeping gene β-actin and...
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Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
... Expression of the type II collagen gene as evaluated by quantitative real-time PCR (D) RT-PCR analysis with the primers recognizing WISP1v (E) A quantitative real-time PCR analysis performed with ... expression levels of the marker genes of chondrocyte differentiation and mineralization by realtime quantitative RT-PCR No significant difference was observed in the mRNA level of type II collagen ... WISP1v and WISP1vx in HCS-2 ⁄ on the differentiation marker genes of growth plate chondrocytes Quantitative real-time PCR analysis was performed with WISP1v and WISP1vx overexpressing HCS-2 ⁄...
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Báo cáo khoa học: Differential effects of histone deacetylase inhibitors on phorbol ester- and TGF-b1 induced murine tissue inhibitor of metalloproteinases-1 gene expression docx
... of Superscript II reverse transcriptase (Invitrogen), according to the supplier’s instructions Quantitative RT-PCR (qRTPCR) was performed using the Applied Biosystems, Warrington, UK) ABI Prism...
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Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf
... http://respiratory-research.com/content/10/1/36 Quantity of TGF-β1 and the percentage of active TGF-β1 in BALF Figure Quantity of TGF-β1 and the percentage of active TGF-β1 in BALF (A–a) The quantity of total and active ... compared to the quantity of total TGF-β1 in the saline control group; *P < 0.05, as compared to the quantity of active TGFβ1 in the saline control group; ΔP < 0.05, as compared to the quantity of ... sample, equivalent to the quantity of TGF-β1 representing active TGF-β1, or treated samples acidified with HCl and subsequently neutralized with NaOH which were equivalent to the quantity of TGF-β1...
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Báo cáo khoa học: Regulatory feedback loop between NF-jB and MCP-1-induced protein 1 RNase pdf
... were visualized by 48 h exposure to a phosphoimage screen and read using molecular imager fx and quantity one software (BioRad, Hercules, CA, USA) EMSA HepG2, HepG2-mock and HepG2-IjBa(S ⁄ A) cell...
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báo cáo hóa học: " Apolipoprotein E expression is elevated by interleukin 1 and other interleukin 1-induced factors" ppt
... intensities were quantified with Scion Image 4.0.3.2 Conditions were established to ensure that maximal cycle number fell within the linear phase of amplification Real-time (quantitative) RT-PCR ... subjected to gel-based RT-PCR for ApoE and GAPDH B: Quantification of the ApoE mRNA (relative to that for GAPDH) by densitometry of the RT-PCR image or by quantitative (real-time) RT-PCR (qRT-PCR); values ... slow-release pellet containing 100 ng IL-1β (IL-1β Pellet); A: semi-quantitative RT-PCR was performed on the RNA B: Results quantified by densitometry showed significant differences in mRNA levels...
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Báo cáo y học: "α Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism" pps
... gels facilitated identification of the enzymes and allowed comparisons between gels The relative quantities of proteolytic enzymes were analysed by scanning densitometry (Umax Colour Scanner, GMbH, ... assay Cell death was measured using the CytoTox 96® assay (Promega, Southampton, UK) This assay quantitatively measures lactate dehydrogenase present in the culture medium that has been released ... active MMP-9 0.2 TNF-α (100 ng/ml) – + + – – C2-ceramide (50 µ µM) – – 2-AP (10 mM) – – – + + – + + Quantitative analysis of (a) MMP-2 and (b) MMP-9 synthesis by bovine articular cartilage Medium...
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Báo cáo y học: "Dynamic compression counteracts IL-1β induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs" pdf
... eliminate any contaminating DNA from the RNA sample (Ambion Applied Biosystems, Warrington, UK) RNA was quantified using the Nanodrop ND-1000 spectrophotometer (LabTech, East Sussex, UK) and stored in ... reactions were prepared for each sample by omitting the Stratascript™ reverse transcriptase Real-time quantitative PCR assays coupled with molecular beacons were performed in 25 μl reaction mixtures ... & Therapy Vol 10 No Chowdhury et al Table Description of the Beacon designer sequences used to quantify gene expression and real-time reaction efficiencies of PCR assays Gene Accession number...
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Báo cáo y học: "Tumor necrosis factor α-induced adipose-related protein expression in experimental arthritis and in rheumatoid arthritis" pptx
... phenylmethylsulfonyl fluoride [PMSF], and 0.5% NP-40) Where indicated, protein concentrations were quantified using the bicinchoninic acid reagent (Pierce, Rockford, IL, USA) Samples (10 μg of total ... 100 μg of anti-TNFα mAb at day ameliorated the disease, as indicated by a rapid fall in the semiquantitative score of arthritis (Figure 3a) [3] To explore the relevance of the therapeutic effect ... of arthritis was associated with upregulation of TIARP mRNA in CD11b+ cells, as demonstrated by quantitative PCR (P < 0.05 at day 7) (Figure 4a) These findings suggest the induction of TIARP in...
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Báo cáo y học: " Effect of HIV-1-related protein expression on cardiac and skeletal muscles from transgenic rats" docx
... column with solvents previously described [11] Fluorescence detection was used for separation and quantification of the dansyl derivatives The redox pairs (i.e., GSH and GSSG, Cys and Cyss) were ... of each annealing and extension step All reactions were performed in triplicate and the starting quantity of the gene of interest was normalized to 18S rRNA for each sample The delta-delta Ct method...
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báo cáo khoa học: " Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death" potx
... expression in rice by quantitative real-time PCR Biochem Biophys Res Commun 2006, 345:646-651 38 Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR ... independent OsHIR1 transgenic lines (Col-0/HIR1-1, Col-0/HIR1-2, and Col-0/HIR1-3) were used for quantitative studies in (b), (c), and (d) and pathogen-inoculated (Figure 4d) plants In both mock-treated ... (expression in untransformed control was set to 1) Error bars show the standard errors (N = 3) (c) Semi-quantitative analysis of OsHIR1 and OsLRR1 electron microscopy signals in the untransformed control...
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Báo cáo y học: "Bone Morphogenetic Protein (BMP)-4 and BMP-7 regulate differentially Transforming Growth Factor (TGF)-β1 in normal human lung fibroblasts (NHLF)" pdf
... transcriptase (Promega UK, Southampton, UK) and μg total RNA in 50 μl volume Real-time quantitative PCR Real-time quantitative PCR was performed using the SYBRGreen JumpStart Taq Ready Mix detection ... stained with Coomassie brilliant blue and analysed Bands were quantified by densitometry with ImageJ software Levels of MMP-13 were quantified in supernatants from NHLF stimulated for 72 h by ELISA ... tenascin C and 250 ng/ml for fibronectin MMP activation and production MMP-1 and MMP-2 activation was quantified by gelatin zymography Proteins of cell supernatants were separated on a 10% acrylamide/0.1%...
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Báo cáo y học: " Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication" ppsx
... target cloned in 3'UTR of pMIR-REPORT™ Expression of the mature hsa-miR-29a and 29b was confirmed by quantitative real-time PCR (Fig 3B) The inserted pre-miRNA disrupted the 5' UTR region of the EGFP ... pEGFP-N3 vector containing pre-miR29a and 29b (B) Expression of hsa-mir-29a and 29b confirmed by quantitative real-time PCR Data show the expression levels of mir-29a and 29b from pEGFP-miR29a ... http://www.retrovirology.com/content/5/1/117 Nef expression, and the cell supernatant was used for HIV1 p24 antigen capture ELISA to quantitatively assess virus production Over-expression of hsa-miR-29a significantly inhibited both...
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Báo cáo y học: " Distinct expression profiles of TGF-β1 signaling mediators in pathogenic SIVmac and non-pathogenic SIVagm infections" pot
... isolation, total RNA extraction from PBMC and reverse transcription were previously described [1] Quantification of t-bet, gata-3, smad3, smad4 and smad7 transcripts was performed by using Taqman ... (Statview, Wilcoxon signed-rank test) [1] http://www.retrovirology.com/content/3/1/37 Results We quantified the expression profiles of pro- and antiinflammatory factors (tnf-α, ifn-γ and il-10) ... pathogenic and non-pathogenic SIV infections In order to search for further early differences, we quantified the transcript levels of t-bet and gata-3, which encode for essential transcription factors...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... b-galactosidase Overexpression of MCPIP was confirmed by western blotting (right upper panel) Semi-quantitative RT-PCR was performed for the luciferase transcript to show the reporter transcript ... overexpressing MCPIP) or endogenous transcripts encoding IL-1b and GADPH was determined by semi-quantitative PCR (with EF-2 as a reference transcript) A total RNA sample (0.1 lg RNA) served as ... 5¢-CCGAGCCACATCGCTCAGAC-3¢ (forward) and 5¢-GTTGAGGTCAATGAAGGGGTC-3¢ (reverse) The relative level of transcripts was quantified by the DDCT method Northern blot analysis For determination of MCPIP and IL-1b transcript...
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Tài liệu Báo cáo khoa học: Transduced human PEP-1–heat shock protein 27 efficiently protects against brain ischemic insult pptx
... Blood Flow Metab 26, 371–381 43 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein–dye binding Anal Biochem 72, 248–254 ... Vector (Burlingame, CA, USA)) They were then visualized by staining with 3,3’-diaminobenzidine Quantitative analysis To calculate the number of surviving neurons, sections (10 per animal) representing...
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Báo cáo khoa học: Adipophilin protein expression in muscle – a possible protective role against insulin resistance pot
... Rockford, IL, USA) Films were scanned with a GS800 densitometer (Bio-Rad) and signals were quantified with Quantity One software (Bio-Rad) The signal intensity of Gapdh or Acta1 was used to calculate...
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Báo cáo khoa học: Insulin like growth factor-1-induced phosphorylation and altered distribution of tuberous sclerosis complex (TSC)1⁄TSC2 in C2C12 myotubes pptx
... used as the cytosolic fraction control IMAGEJ software was used for quantification Protein contents in IGF-1-stimulated sample were quantified as the relative amount compared to the simultaneously ... the cytosolic fraction Alterations in the relative amounts of both TSC1 and TSC2 proteins were quantified as a percentage value compared to the control group in each fraction using IMAGEJ software ... pan-14-3-3 antibody precipitates both TSC1 and TSC2 (B) Levels of TSC2 bound to 14-3-3 proteins were quantified using IMAGEJ software (n = plates per condition) Each value is the mean ± SE *Significant...
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