một số nét về đa dang sinh học vườn quốc gia ba vì
Mechanisms in dendrite pruning of drosophila dendritic arborization neurons
... the Xba I linearized pUAST vector with the Xba I site at 5’ end and the Nhe I and Xba I sites at 3’ end The correct pUAST 3HA-FM plasmid was linearized with the Nhe I enzyme and the pUAST backbone ... mixed, centrifuged briefly and incubated for hour at 37°C At the end of the incubation 2μl DNaseI, RNase- 22 free (Roche) was added to the mixture and incubated for 15 at 37°C to remove the DNA ... and 18 h APF stages The number of samples (n) in each group is above the bars Error bars represent standard deviation Scale bar, 50 μm of to primary and secondary dendrites of ddaCs in mical15256...
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Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
... 150 125 100 75 50 25 – + * 17 16 250 200 14.3 kDa band 13.3 kDa band 12.5 kDa band 150 100 50 0 BACE1 KO WT BACE1 Tg BACE1 KO WT BACE1 Tg Fig BACE1 expression decreases FLAPP steady-state levels ... wild type) 55 250 140 120 100 80 60 40 20 BACE1 KO WT BACE1 Tg 14.8 kDa band 13.4 & 12.6 kDa bands 9.6 kDa band 200 150 100 50 BACE1 KO WT BACE1 Tg Fig BACE1 expression decreases FLAPLP2 protein ... type) FL APLP1 (% of wild type) 8.2 kDa – 7.8 kDa bands 5.9 kDa band D 250 200 150 100 50 150 100 50 BACE1 KO WT BACE1 Tg BACE1 KO WT BACE1 Tg Fig BACE1 expression decreases FLAPLP1 levels and gives...
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Báo cáo hóa học: " Human cytomegalovirus UL27 is not required for viral replication in human tissue implanted in SCID mice" ppt
... X17403) Two additional viruses were constructed by one of us (GK) in the bacterial artificial chromosome, AD169-BAC One virus, ∆UL27BAC deletes the amino terminal 90% of UL27 and the carboxyl terminal ... implants in SCID-hu mice Virus Days post infection AD169 (ATCC)a T2092-1-1-7 AD169 RVc ∆UL27BAC 1/3∆UL27BAC 16 28 42 3.0 ± 0.58 (4/12)b ± (0/12) 2.6 ± 0.17 (4/12) ± (0/12) ± (0/12) 3.5 ± 1.3 (5/12) ... genomes, restriction fragments were compared to the AD169-BAC and the predicted changes in fragment sizes were observed No other alternations in the BAC DNA were apparent The coordinates of the deletions...
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Báo cáo hóa học: " Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia" pot
... incubated with a monoclonal mouse anti-heparan sulfate (10E4 epitope) antibody diluted 1:100 in PBS followed by a Cy3 conjugated donkey anti-mouse IgG diluted 1:200 (Jackson Immunoresearch, Bar ... replaced with fresh media After a 48-hour incubation, cells were treated for 15 minutes with 400 µl of enzyme-free cell dissociation buffer (Gibco, Carlsbad, CA) and lifted from plates The suspension ... 2000, 269:137-147 Bartlett JS, Wilcher R, Samulski RJ: Infectious entry pathway of adeno-associated virus and adeno-associated virus vectors J Virol 2000, 74:2777-2785 Summerford C, Bartlett JS, Samulski...
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Báo cáo y học: "Wildtype epidermal growth factor receptor (Egfr) is not required for daily locomotor or masking behavior in mice" pps
... Laboratory (Bar Harbor, ME) The derivation of the Egfrwa2 congenic lines involved backcrossing the Egfrwa2 allele for greater than ten generations to C57BL/6J and 129S1/SvImJ genetic backgrounds ... mixed genetic background In order to resolve the discrepancies implicating a critical role for EGFR in locomotor and masking behaviors, as well as to examine potential genetic background effects ... results Since the animals were not properly controlled for genetic background, numerous other interpretations (such as unequal genetic background distribution in control and test mice) most likely...
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Báo cáo Y học: The cytoplasmic C-terminus of the sulfonylurea receptor is important for KATP channel function but is not key for complex assembly or trafficking pdf
... before incubation with mL 0.5 mgÆmL)1 EZ-Link Sulfo-NHS-LCBiotin (Pierce, Rockford, Illinois, USA) in NaCl/Pi at °C for 30 Dishes were washed twice with ice-cold NaCl/Pi before incubation with ... subsequently incubated at 65 °C for 10 before dilution with mL 1% (v/v) Triton in Tris/NaCl containing mM phenylmethanesulfonyl fluoride and the protease inhibitor cocktail Lysates were then incubated on ... After the incubation period, the binding resin was pelleted by centrifugation (20 000 g for at °C) followed by five washes with mL binding buffer Bound protein was recovered by incubation with 80...
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Báo cáo y học: "JNK1 is not essential for TNF-mediated joint disease" doc
... 7.4) for 10 This was followed by 30 of incubation with a biotinylated antirat IgG secondary Ab (Vector, Burlingame, CA, USA) Then, sections were incubated with the appropriate VECTASTAIN@ABC reagent ... osteoclasts was similar in the two animal groups (b) Vertical bars indicate standard deviation JNK, c-Jun N-terminal kinase destruction Evidence from basic and clinical research has established TNF-α as ... 400×) Results of quantification (percentages of positive cells) are given in separate bar charts Vertical bars indicate standard deviation JNK, c-Jun N-terminal kinase; p-c-Jun, phosphorylated...
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Báo cáo y học: "Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 " ppt
... D) Blots were reprobed for ERK2 as loading reference (D) * indicates the hCycT1-specific band The lower band seen in all splenocyte samples was considered non-specific # gives the ID numbers of ... closely related transgene vector backbone, respectively [12], and with the targeted expression of hCycT1 in tg mice [10,25,26] Furthermore, it provides the conceptual basis to generate potentially ... HIV-1 in Supernatant (ng p24/ml) 30 20 10 70 60 Ba- L 50 40 the employed HIV-1 strain and HIV-1R7/3 YU-2 Env GFP is superior to the HIV-1NL4-3-based virus The steady rise of the percentage of...
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Báo cáo y học: " The carbohydrate at asparagine 386 on HIV-1 gp120 is not essential for protein folding and function but is involved in immune evasion" potx
... LuSIV cells were incubated with the two viruses and luciferase was measured after 24 hours RLU, relative light units Error bars represent standard deviations B DCs were incubated for hours with ... only a single band corresponding with the preprotein form of gp120 (Reduced uncleaved = Ru) was present directly after the pulse (fig 3) After hrs of chase, however, a prominent band just below ... HIV-1LAI virus and wt and N386D pseudovirus derived from HIV-1JR-FL were preincubated with antibody and subsequently incubated with TZM-bl cells containing a luciferase reporter construct under control...
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Báo cáo y học: "Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue" docx
... fragment was cloned in the pECFP-C1 vector backbone (Clontech) An untagged tetherin plasmid was cloned by amplification of tetherin with primers introducing XbaI and MluI sites and subcloning in the ... CD4+ T-cell depletion in human lymphoid tissue ex vivo J Virol 2004, 78:12689-12693 Glushakova S, Baibakov B, Margolis LB, Zimmerberg J: Infection of human tonsil histocultures: a model for HIV pathogenesis ... KA, Votteler J, Schubert U, Bibollet-Ruche F, Keele BF, Takehisa J, Ogando Y, Ochsenbauer C, Kappes JC, Ayouba A, Peeters M, Learn GH, Shaw G, Sharp PM, Bieniasz P, Hahn BH, Hatziioannou T, Kirchhoff...
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Báo cáo y học: " Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2" ppsx
... doxycycline treatment and the cells incubated with ZEBOV-GP-Fc or podoplanin-Fc, respectively, in the presence of PBS (dark bars) or mM EDTA containing FACS buffer (white bars) Bound proteins were detected ... stained with 18H5 (B) PBMCs were incubated with μM staurosporine for the indicated times and podoplanin expression (black bars) and annexin V binding (white bars) were determined by flow cytometry ... in HIV Therapy Edited by: Reeves JD, Derdeyn CA Basel: Birkhäuser Verlag; 2007:31-47 14 Cameron PU, Freudenthal PS, Barker JM, Gezelter S, Inaba K, Steinman RM: Dendritic cells exposed to human...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... nucleotide through complementary base-pairing between the snoRNA and the rRNA substrate The snoRNAs involved in rRNA modification can be divided into two major classes based on conserved sequence elements ... lane The figures show autoradiographs of RNA transferred to nylon membranes incubated in En3Hance (Amersham Biosciences) Bands corresponding to major intermediates and mature rRNAs are indicated and ... incubated in glucose medium for different time intervals and hybridized against specific oligonucleotide probes The relative positions of the probes on the 35S pre-rRNA are indicated in (B) Bands...
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Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
... (stippled bars), PKRD6 (dark bars), or eIF2a-S51A (hatched bars) on the differentiationinduced IRES activation relative to the fold induction value with GFP that was set as 100% (light bars) (B) ... fused to a fragment encoding the cytosolic form of firefly luciferase from pGL3-basic (Promega) as the second cistron A 22-base pair fragment containing StuI and NcoI sites separates the stop codon ... NaCl and 0.5 lL anti-eIF2a mAb [21] and incubated for h at °C Next, rabbit anti-(mouse IgG) Ig (Jackson Immuno Research) were added for further incubation of h, followed by addition of 10 lL packed...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
... cysteine was introduced into the periplasmic loop of MscL at position 54 (MscL F54C) Based on the structure of the Mycobacterium tuberculosis MscL homologue, this position is expected to be exposed ... collected and incubated for 10 in buffer containing EDTA This treatment permeabilizes the outer membrane to facilitate access of AMS, which was added subsequently After of incubation, unbound AMS ... apparent molecular mass of 180 kDa (arrow) band at 180 kDa that presumably represents the MscL–HA pentamer The aberrant electrophoretic mobility is probably due to binding of the detergent (DDM)...
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Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx
... conserved histidine that is probably part of the catalytic triad of the active site (Fig 1B) The third member of the catalytic triad could not be identified by sequence-based searches PTS1-dependent ... RP-HPLC [14] Proteins in individual HPLC fractions were further separated by SDS–PAGE, and protein bands were cut out and analysed by mass spectrometry Lpx1p (systematic name: Yor084wp) was extractable ... soluble thiolase Fox3p (EC 2.3.1.x) than of Lpx1p appears to be present in the supernatant This is probably due to partial peroxisome rupture during preparation, and might indicate that Lpx1p, in contrast...
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Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt
... mitogen-activated protein kinase Cancer Res 61, 3084–3091 Shimada M, Andoh A, Araki Y, Fujiyama Y & Bamba T (2001) Ligation of the Fas antigen stimulates chemokine secretion in pancreatic cancer cell ... Khosravi-Far R, Chang HY & Baltimore D (1997) Daxx, a novel Fas-binding protein that activates JNK and apoptosis Cell 89, 1067–1076 Chang HY, Nishitoh H, Yang X, Ichijo H & Baltimore D (1998) Activation ... activation is not required for FasL-induced NF-jB activation Conversely, the transient expression of IjBa-SR, which dramatically reduced the FasLinduced NF-jB activation (Fig 2B and [10]), caused little...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
... proteins were transformed into BL21 bacteria Expression of fusion proteins was induced by incubation with 0.1 mm isopropyl thio-b-dgalactoside (IPTG) for h at 25 °C The bacteria were harvested by centrifugation, ... corresponding arginine (Arg45) to Ala in PTP1B led to very low catalytic activity, probably as a result of structural perturbation of the catalytic site [42] As seen from the crystal structure of RPTPa-D2 ... Saito H & Frederick CA (2005) Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45 J Exp Med 201, 441–452 Debant A, Serra-Pages C, Seipel K, O’Brien...
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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... plants [Arabidopsis thaliana (ARATH) and Nicotiana tabacum (TOBAC)], algae [Chlamydomonas reinhardtii (CHLRE) and Porphyra purpurea (PORPU)] and cyanobacteria [Synechcoccus elongatus (SYNEL) and Prochlorococcus ... The knock-out allele was introduced into the tobacco plastid genome by particle bombardment-mediated chloroplast transformation [21] From 10 bombarded leaf samples, 19 chloroplast transformants ... genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids DNA Res 10, 137–145 29 Inoue H, Tsuchiya T, Satoh S, Miyashita H, Kaneko T, Tabata S, Tanaka A & Mimuro...
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Báo cáo khoa học: Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation potx
... of pSKBYD1.4 P, PstI; Xb, XbaI; S, SacI; B, BamHI; E, EcoRI; C, ClaI; Bg, BglII; C1, C2, V1 and V2 represent complementary and virion sense ORFs, respectively The bar represents 500 bp The intron ... [46] Lane 1, extracts from cells bombarded with p35SHA6HISRep alone; lane 2, extracts from cells bombarded with p35SHARepA alone; lane 3, extracts from cells bombarded with both p35SHA6HISRep and ... agar plates and preincubating the cells for 3–4 days prior to bombardment pBYD1.4mer and pDintron were generously provided by J Stanley (John Innes Centre, Norwich, UK) For bombardments, one micron...
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