multiscale in situ measurements of intertidal benthic production and respiration
in situ detection of dna damage, methods and protocols
... Detection In Situ: Potentials and Pitfalls The development of techniques for in situ end-labeling (ISEL) of fragmented DNA has allowed the recognition of DNA damage in single cells both in vitro and in ... colorimetric and fluorescent staining of cultured cells and tissues is presented thereby allowing investigators to utilize the TUNEL staining procedure with a minimal investment in laboratory reagents and ... 3.3.3 Labeling of 5'-OH Ends of DNA Prepare a reaction tube by adding µL of 10× T4 kinase reaction buffer, µL of sample from step of Subheading 3.3.1., µL (50 Units) of T4 Kinase (New England BioLabs,...
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Applying method of project based teaching in teaching knowledge of electric power production and use for high school students
... subject and scope Research subject: Teaching and studying activities in teaching by project method Research scope: Teaching and studying activities in teaching by project method in production and ... According to Do Huong Tra (2011), include: Finding ideas of the project; Defining targets of the project; Building orienting questions; Designing the project; Plan of teacher and students; Supporting ... method of the teacher, including: proposing ideas, assigning tasks, and grouping, assigning works, inspecting, supporting students and evaluating results 1.2.11 Evaluation in teaching by project method...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
... distributions of in situ nitrifying activities were determined The relationship between the spatial organization of nitrifying bacterial populations and the in situ activity of these populations within ... Satoh, and Y Watanabe 1999a In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes Appl Environ Microbiol 65: 3182-3191 Okabe, S., H Satoh, and ... of Water and Environment Technology, Vol.2, No.2, 2004 Therefore, we investigated successional development of nitrifying bacterial community structure and in situ nitrifying activities in biofilms...
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The Role of Rainfed Agriculture in the Future of Global Food Production doc
... agricultural research; and reforming policies and increasing investments in rainfed areas WATER HARVESTING Water harvesting involves concentrating and collecting the rainwater from a larger catchment ... being grown Types of external catchment systems include runoff farming, which involves collecting runoff from the hillsides into flat areas, and floodwater harvesting within a streambed using ... harvesting takes place In addition, most water harvesting operations consist of a catchment area and a receiving area for the capture of runoff, and are generally small both in size and in level of...
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in situ photography of picosecond laser ablation of nickel
... result in phase explosion, resulting in the ejection droplets of superheated liquid Both of these processes will result in scattering and attenuation of the probe beam On the other hand, the probing ... are seen in the photographs of Fig at an average fluence of 5.3 J/cm2 At this fluence probe beam attenuation beginning at À30 ps and the attenuated region is fully expanded to a diameter of approximately ... shown in the final photograph at in nity Photographs at an average fluence of 121 3.1 J/cm2 are shown in Fig Probe beam attenuation begins at À10 ps and expansion of the attenuated region continues...
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Báo cáo hóa học: " In situ Control of Si/Ge Growth on Stripe-Patterned Substrates Using Reflection High-Energy Electron " potx
... line in Fig 4g) The latter is caused by the surface roughening of the 2D Ge wetting layer surface associated with the formation of a high density of dimer vacancy lines and subsequently missing ... intensity evolution of the specular spot and two 3D diffraction spots indicated by the dashed square and circle in (f) as a function of Ge coverage Accordingly, an onset of ripple formation and ... Right: Intensity evolution as a function of coverage of 123 the specular spot, 3D ripple, and 3D island spot (purple, blue, and red line, respectively) indicated by the dashed square and circle in...
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Báo cáo hóa học: " In Situ Mineralization of Magnetite Nanoparticles in Chitosan Hydrogel" ppt
... result from TEM Principle of In Situ Mineralization of Magnetite in Chitosan Hydrogel Fig TG curves of pure magnetite (a) and the mineralized magnetite nanoparticles (b) existence of chitosan layer ... on mineralization of magnetite in organic hydrogel have great scientific and practical significance Inspired by magnetic bacteria, we propose in situ mineralization of magnetite nanoparticles in ... iron ions and chitosan The XPS spectra of chitosan and CS–Fe complex were shown in Fig The binding energies for N have a significant change between chitosan and CS–Fe complex The N s band of chitosan...
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Báo cáo hóa học: " In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release" ppt
... water -in- oil microemulsion is developed to encapsulate the bFGF within MSNs in situ The in vitro releasing kinetics of bFGF from MSNs has been investigated through colorimetric enzyme linked ... determined by ELISA kit; M0, the original amount (wt) of bFGF, that is, 10 lg In Eq 1, C0 refers to the original concentration of bFGF in aqueous phase of microemulsion, i.e 10 lg in 0.1 mL, and ... buffer, and the tunable nano-channels in MSNs as showed in Fig 2b Our efforts are continuously focusing on how to control the initial burst and increase the loading efficiency On the other hand,...
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Báo cáo hóa học: " In Situ Generation of Two-Dimensional Au–Pt Core–Shell Nanoparticle Assemblies" pptx
... analysis software Surface coverage of different nanoparticles was estimated by analyzing the mean grain area and number of grains present in a typical lm lm (total area = lm2) AFM image of the respective ... adsorbed ions during bimetallic NP formation, considering the difference in ionic charges of the respective ions and electrostatic interactions working in the process Water contact angle measurements ... surfaces and sensors The earlier discussion of AFM results points to the possible core–shell structure formation during in situ synthesis of Au–Pt NPs on the surface from the observed increase in nanoparticle...
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Báo cáo hóa học: " In situ Polymerization of Multi-Walled Carbon Nanotube/Nylon-6 Nanocomposites and Their Electrospun Nanofibers" pot
... spectra of (a) P-MWNTs and (b) F-MWNTs In situ Polymerization In situ polymerization of e-caprolactam in the presence of the F-MWNTs and the P-MWNTs was carried to prepare the F-MWNT/nylon-6 and ... formation of extended chain crystals and is typically obtained from a process involving elongational flow [47] The reflections at 2h = 11 and 21° are -201/200/001 and 020 of the c form, respectively, and ... and methanol, and were finally dried under a reduced pressure for days at 100 °C In situ Polymerization of the Nanocomposites In situ polymerization of e-caprolactam in the presence of F-MWNTs (or...
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Báo cáo y học: "Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+ T cells from patients with rheumatoid arthritis" ppsx
... cytokine production, and IL-10 can directly suppress this response by inhibiting CD28 tyrosine phosphorylation and binding of phosphatidylinositol 3kinase [24] To evaluate the responsiveness of ... defective IL-10 inhibition of CD28-costimulated production of both IFN-γ and IL-2 Numerous cytokines, both proinflammatory and anti-inflammatory, have been detected in the ST of RA, and the balance ... phosphatase-2-binding domain of receptors such as gp130 [40] SOCS1 and SOCS3 are induced by various cytokines, including IL-6 and IL-10, as mediators of negative feedback and crosstalk inhibition [7]...
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Báo cáo lâm nghiệp: "In situ measurement of leaf water use efficiency of lilac (Syringa vulgaris): comparison with crop plants" docx
... be induced by: 1) individual variability 2) changes in A between morning and evening at the same level of incident and PPFD The maximum values for potato about the same as those for lilac are increases ... The data shown in following figures correspond to a typical day for each species Each symbol represents a leaf on different plants in the field for maize and potato, and of trees in a hedge for ... between versus 0.20 g, Up to and 0.23 , s m’ mol the g dependence of A is c c almost linear and the slope of this line represents C Beyond these values, i - C a A does not increase for both C plants,...
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Báo cáo y học: "CD147 overexpression on synoviocytes in rheumatoid arthritis enhances matrix metalloproteinase production and invasiveness of synoviocytes" pptx
... predominantly on the MLS and FLS in the lining and sublining layers of RA synovium, and macrophages acted as the amplifier of the pathogenetic cascade in RA via the increase of MMP production by interacting ... important in both autocrine and paracrine stimulation of MMPs From the findings that homophilic CD147 binding has occurred in the context of both heterotypic and homotypic cell–cell interactions and ... characteristic of RA These findings, together with a better understanding of the relationship between CD147 and RA, and of the possible mechanism and regulation of the effect of CD147 on MMP production, ...
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Báo cáo y học: " Pharmacology of airways and vessels in lung slices in situ: role of endogenous dilator hormones" ppt
... arteries and veins to histamine and 5-HT in lung explants of guinea-pigs Br J Pharmacol 1998, 123:1525-1532 Martin C, Uhlig S, Ullrich V: Videomicroscopy of methacholineinduced contraction of individual ... airways, whereas veins where at a distance from them, and 2) arterial walls had a thick media and their inner lining was slightly wrinkled, whereas veins were thinner and wrinkles were inconspicuous ... indomethacin (10-5 M) or the combination of apamin and charybdotoxin (5 × 10-7 M and 10-7 M) which together inhibit EDHF release (18) For these experiments incubations were continued for 10 minutes...
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Báo cáo y học: " In situ detection of Gag-specific CD8+ cells in the GI tract of SIV infected Rhesus macaques" pptx
... multimer is low Staining pattern and staining intensity of Gag CM9 Qdot 655 multimer positive cells Confocal microscopy revealed a punctate staining pattern of individual cells stained with the Gag ... availability of a sensitive and specific technique for in situ localization of virus specific CD8+ T cells may prove useful in the study of the pathogenesis of SIV infection and the role vaccines and ... modeling of the staining pattern using Volocity (Improvision) software revealed the close proximity between CD8 molecules and the T cell receptor (Fig 2E, G) The CD8 staining and the Gag CM9 staining...
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Báo cáo sinh học: "Phenotypic plasticity of body pigmentation in Drosophila: Detection of on cytoplasmic production" pptx
... simulation of a dairy cow population with cytoplasmic effects but their interest was in biases introduced through ignoring cytoplasmic effects, and not in the detection of cytoplasmic variance In this ... that both c and m were Fixed effects included in the model were year of record and sex A single record at hogget age was analysed For the fitting of the cytoplasmic effect the female in the base ... studies following that of Bell et al (1985) have presented evidence for the influence of cytoplasm on production in cattle However, Kennedy (1986) pointed out that careful analysis of data was...
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In situ spectroscopic of the early events in the rodium mediated pauson khand reaction 2
... Allian, Ayman D.; Tjahjono, Martin and Garland, Marc In- situ mid-Infrared Vibrational and Kinetic Study of the fragmentation of the ([(1-heptyne)Rh4(CO)7(μ-CO)2 (Manuscript in preparation) Selected ... G.3 Far-infrared Region A representative spectra of the far-infrared region, 400-700 cm-1, are shown in Figure G.1 Figure G.1 In- situ raw far-infrared spectra of the hydroformylation of 3,3-dimethyl-1-but-ene ... comparison of predicted and experimental and bond lengths and bond angles and (3) predicted and experimental mid-infrared spectra All are in very good agreement Furthermore, the dihedral angle of butterfly...
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In situ spectroscopic of the early events in the rodium mediated pauson khand reaction
... designing and machining of flow through high/low pressure reactors and spectroscopic cells, (2) using various spectroscopic tools (IR and Raman and NMR) (3) carrying out blind deconvolution of the ... achieved by a single individual, therefore, I was very fortunate to be part of very competent team at the National University of Singapore and Institute of Chemical and Engineering Sciences (ICES) ... pre-catalytic events involved in the Rh4(CO)9(µCO)3 mediated Pauson Khand reaction using in- situ spectroscopy In particular, the investigation focused on understanding the (i) catalytic precursor and (ii)...
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Cell culture microchip with built in sensor array for in situ measurement of cellular microenvironment
... BUILT -IN SENSOR ARRAY FOR IN- SITU MEASUREMENT OF CELLULAR MICROENVIRONMENT ZHANG LIN (B.Eng, ZJU, China) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF BIOENGINEERING ... encapsulation of these sensors β-TC-6 cell was used as the cell line cultured in the final microfluidic device and in- situ measurement of three parameters including pH, oxygen and glucose concentration in ... 25 2.4.3 Fusion of Microfluidics and Optics Very recently, there’s the trend of development of optofluidics, the fusion of microfluidics and optics [47] The idea of optofluidics initially refers...
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In situ distribution of enolase isozymes in chronic liver disease
... a-Enolase was found in almost all hepatocytes and epithelial cells of hile ductules and interlobular and septal bile ducts in all five cases The intensity of the staining of this antigen in bile duct ... Grant -in- Aid for Encouragement of Young Scientists ofthe Ministry of Education, Science, and Culture of Japan Reprint requests: Yoshihide Fukuda, M.D Second Department of Internal Medicine Nagoya ... results of the distribution of the isozymes in various cells are summarized in Table Chronic active hepatitis and cirrhosis Staining patterns of a- and ^-enolases were almost the same in chronic...
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