... fluorescent protein Constructs expressing full-length and C-terminal (KKKKKKSKTKCVIM) domain of human K- Ras (K- Rasfull and K- RasC14) fused to green fluorescent protein (GFP) and to its spectral ... targeting of K- Ras B C D E Fig Protein expression and subcellular localization of YFP -K- RasC14 and YFP -K- Rasfull in CHO -K1 cells (A) Homogenates from CHO -K1 cells expressing YFP, YFP -K- RasC14 or YFP -K- Rasfull ... YFP and YFP -K- RasC14 as bands of 27 kDa and 27.5 kDa, respectively, and YFP -K- Rasfull as a band of 55 kDa according to the expected molecular mass (Fig 1A) Membrane association of the expressed...
... subfractions of LF from seven samples of different human milk Results are mean ± SD from three measurements for each of seven LF preparations Substrate Km (lM) 103 · kcat (min)1) 10)3 · kcat/Km (min)1ÆM)1) ... 177–184 Kanyshkova, T.G., Semenov, D.V., Buneva, V.N & Nevinsky, G.A (1999) Human milk lactoferrin binds two DNA molecules with different affinities FEBS Lett 451, 235–237 Nevinsky, G.A., Kanyshkova, ... A from human milk catalyzes milk protein phosphorylation Appl Biochem Biotechnol 75, 77–91 Akagi, K. , Murai, K. , Hirao, N & Yamanaka, M (1976) Purification and properties of alkaline ribonuclease...
... distribution of rat sarcosine dehydrogenase Eur J Biochem 257, 556–561 Noshiro M, Usui E, Kawamoto T, Kubo H, Fujimoto K, Furukawa M, Honma S, Makishima M, Honma K & Kato Y (2007) Multiple mechanisms ... role of the ‘spot 14’ protein in the regulation of lipid synthesis J Biol Chem 270, 16615–16618 Laustsen PG, Michael MD, Crute BE, Cohen SE, Ueki K, Kulkarni RN, Keller SR, Lienhard GE & Kahn ... cholesterol uptake, the upregulation of Ldlr and Scarb1 suggested enhanced uptake of cholesterol in liver of rats in group H Furthermore, downregulation of Hmgcr suggested inhibition of endogenous...
... values of m and Dm The curve fittings were performed using MICROSOFT ORIGIN software Calculation of the rate constants from the kinetics of refolding HSA was found to follow two phases of refolding, ... of Nile red Ó FEBS 2004 Mechanism of folding and unfolding of HSA (Eur J Biochem 271) 1795 Fig Equilibrium refolding of HSA in the presence of different concentrations of GdnHCl The recovery of ... discrepancy in the rate constants of the slow phase may be due to a difference in the rates of folding of the local environment of the probes Taken together, the results of the kinetic studies suggested...
... nm) of the reaction products (AII for the hydrolysis of AI; Phe-Arg for the hydrolysis of BK, Ile-Ser-BK and [Thr6]BK; TyrArg for the hydrolysis of [Tyr8]BK; MKRSRGPSP for the hydrolysis of MKRSRGPSPRR; ... figp The Ki of inhibition of ANCE by [Thr6]BK was determined by measuring the kinetics of Abz-YRK(Dnp)P hydrolysis in the presence of 0, 10, 20, 50 and 80 nm [Thr6]BK Structure-activity of Drosophila ... hydrolysis of the peptide and no inhibition of the cleavage of Abz-YRK(Dnp)P by ACER ANCE ACER Substrate BK (RPPGFSPFR) Ile-Ser-BK (ISRPPGFSPFR) [Tyr8]BK (RPPGFSPYR) [Thr6]BK (RPPGFTPFR) MKRSRGPSPRR...
... to Profit and Risk College Park (MD): Department of Agricultural and Resource Economics, University of Maryland Working Paper 03-10 Hanson JC, Johnson DM, Peters SE, Janke RR 1990 The profitability ... amount of organic matter in the upper 15 cm of soil in the organic farming systems was approximately 110,000 kg per The soil of the upper 15 cm weighed about 2.2 million kg per Approximately 41% of ... Smolik and colleagues (1995) found that corn yields in South Dakota were somewhat higher in the conventional system, with an average yield of 5708 kg per ha, compared with an average of 4767 kg...
... Industrial Research, India [grant no 37/(1071)/01-EMR-II] The authors would like to thank Professor S Basak of SINP, Kolkata for extending help in conducting the time-resolved fluorescence experiments, ... properties of a transcriptional activator: the cII protein of phage J Biol Chem 257, 9128–9134 Shotland, Y., Koby, S., Teff, D., Mansur, N., Oren, D.A., Tatematsu, K. , Tomoyasu, T., Kessel, M., Bukau, ... structural aspects of limited proteolysis of native proteins Biochim Biophys Acta 1382, 191–206 26 Karata, K. , Verma, C.S., Wilkinson, A.J & Ogura, T (2001) Probing the mechanism of ATP hydrolysis...
... Ye Y, Godzik A: Database searching by flexible protein structure alignment Prot Sci 2004, 13:1841-1850 Kohno T, Goto T, Takasaki T, Morita C, Nakaya T, Ikuta K, Kurane I, Sano K, Nakai M: Fine ... complex Figure Comparisonsof the VSV and FLUAV N structures Comparisonsof the VSV and FLUAV N structures Stereo cartoon drawings for the superposition of the C-terminal domain of the FLUAV N ... C-terminal end of the FLUAV N protein RNA binding cavity Since structures of both BDV and FLUAV N proteins were determined in the absence of RNA, comparisons to the structure of the N-RNA complex of VSV...
... 85:531-534 Ito Y, Joklik WK: Temperature-sensitive mutants of reovirus I Patterns of gene expression by mutants of groups C, D, and E Virology 1972, 50:189-201 Schuerch AR, Joklik WK: Temperature-sensitive ... § ‡ ‡ PQIVSDMINRKRLRVHTPSDRRAAQLMHLPYHVKRGASHVDVYKVDVVDVLLEVVDVADGLRNVSRKLTMHTVPVCILEMLGIEIADYCIRQEDGMFTDWFLLLTMLSDGLTDRRTHCQYLINPS F LL LQ SS NE KI I T R F IK S LQ SVI LI L T K N I S M F L M ... V M V V 560 P 570 580 590 600 610 †∗ 620 † PLAKDLLAPMARKFYDNSMSEGRLLTFAHADSELLNANYFGHLLRLKIPYITEVNLMIRKNREGGELFQLVLSYLYKMYATSAQPKWFGSLLRLLICPWLHMEKLIGEADPASTSAEIGWHIPRE V H EE L F M M D A I S...
... Ye Y, Godzik A: Database searching by flexible protein structure alignment Prot Sci 2004, 13:1841-1850 Kohno T, Goto T, Takasaki T, Morita C, Nakaya T, Ikuta K, Kurane I, Sano K, Nakai M: Fine ... complex Figure Comparisonsof the VSV and FLUAV N structures Comparisonsof the VSV and FLUAV N structures Stereo cartoon drawings for the superposition of the C-terminal domain of the FLUAV N ... C-terminal end of the FLUAV N protein RNA binding cavity Since structures of both BDV and FLUAV N proteins were determined in the absence of RNA, comparisons to the structure of the N-RNA complex of VSV...
... 85:531-534 Ito Y, Joklik WK: Temperature-sensitive mutants of reovirus I Patterns of gene expression by mutants of groups C, D, and E Virology 1972, 50:189-201 Schuerch AR, Joklik WK: Temperature-sensitive ... § ‡ ‡ PQIVSDMINRKRLRVHTPSDRRAAQLMHLPYHVKRGASHVDVYKVDVVDVLLEVVDVADGLRNVSRKLTMHTVPVCILEMLGIEIADYCIRQEDGMFTDWFLLLTMLSDGLTDRRTHCQYLINPS F LL LQ SS NE KI I T R F IK S LQ SVI LI L T K N I S M F L M ... V M V V 560 P 570 580 590 600 610 †∗ 620 † PLAKDLLAPMARKFYDNSMSEGRLLTFAHADSELLNANYFGHLLRLKIPYITEVNLMIRKNREGGELFQLVLSYLYKMYATSAQPKWFGSLLRLLICPWLHMEKLIGEADPASTSAEIGWHIPRE V H EE L F M M D A I S...
... (32) n μ⊥ n k α.n > 0.4 Page of μ⊥ +1 n k (36) B The rank deficiency of Rna According to (36), the rank of Rna is such that rank Rna = k μ⊥ + + μ⊥ n k (37) Therefore, the rank deficiency of Rna, ... Algorithm 1: Estimation ofk and μ⊥ Input: Value of n and na ˆ ˆ ˆ Output: Value ofk and μ⊥ ˆ for k = to n − 1do ˆ for Z = to n − k ˆ μ⊥ = μ⊥ na − k − Z ; ˆ ˆ ˆ n ˆ ˆ k= kk ; end end It is done ... matrices of size (L × l) Algorithm 2: Estimation of (n − k) parity check ˆ ˆ Input: y, n, k and μ⊥ ˆ ˆ ˆ Output: (n − k) parity check ˆ ˆ for s = to (n − k) ˆ ˆ xs = y1 (t) · · · yk (t) yk+s (t)...
... (1) /burorororo/ [b o o o o], (2) /karakarakarakarakarakorokorokorokorokoro / [ka aka aka aka aka ako oko oko oko oko o] (1) /potyaN/ [pot an], (2) /pikori/ [piko i] (1) /waN/ [wan], (2) /wauQ/ ... crying sound of a flowing stream sound of a noisy construction site (mainly the machinery noise of a jackhammer) sound of fireworks sweeping tone knock (knocking on a hard material like a door, twice) ... /tyurororo/ [t Ï È ËÏ (1) /riN/ [ in], (2) /kiriiN/ [ki i: n] (1) /goo/ [ o:], (2) /zaaaaa/ [dza:] (1) /katsukotsu/ [kats kots ], (2) /kotoQ kotoQ/ [koto koto ] Ï Ï È È EURASIP Journal on Audio,...
... Tλ,μ , BbR ∩ K, K i Tλ1 ,μ1 , BbR ∩ K, K 4.7 By the additive property and 4.4 , 4.7 , we have i Tλ,μ , BbR Ω ∩ K, K −1 4.8 Therefore, 3.20 has another positive solution in BbR \Ω K at λ, μ ∈ ... ⊂ Ω2 Let T : K ∩ Ω2 \ Ω1 → K be a completely continuous such that either i ii T u ≤ u for u ∈ K ∩ ∂Ω1 and T u ≥ u for u ∈ K ∩ ∂Ω2 or T u ≥ u for u ∈ K ∩ ∂Ω1 and T u ≤ u for u ∈ K ∩ ∂Ω2 Then ... fixed point in K ∩ Ω2 \ Ω1 Theorem 2.7 Let X be a Banach space, K a cone in X and Ω bounded open in X Let ∈ Ω and T : K ∩ Ω → K be condensing Suppose that T x / νx, for all x ∈ K ∩ ∂Ω and all...
... k − j j a k j 1−q q k a qm k j x −1 a qm a 1−q j m a k j j − qm k j ∞ −1 j −1 k j j j j a j 1−q a k j k j −1 j j j a k m k j r−1 m q m j m m m k r−1 m m m m k m m k qm x m 1−q j k j k j r qa ... r,q x k r ∞ r −1 −1 r−1 m m m m m q m m r ∞ r r−1 m q ∞ r −1 q ∞ r −1 r k j r k r kk a j j j a a a −1 m m x 1−q m j qm k j x j j x k j x k j qm a k j j r−1 m m 4.1 k j qa k j r x a, j − a, k − ... interpolation function of the generating functions of higher order q-Genocchi polynomials From 1.20 , we easily see that ∞ k ∞ tk k! r Gk,q x r r! kk ∞ r r −1 r−1 m m m q m m m x k tk r k r ! 3.1 From...
... minimum m, define Tk T − {m}; otherwise set Tk T If T has a left scattered maximum M, define Tk T − {M}; otherwise set Tk T Definition 2.2 For f : T → R and t ∈ Tk , the delta derivative of f at the point ... to the best of our knowledge, few people considered the second-order dynamic equations of increasing homeomorphism and positive homomorphism on time scales For the existence problems of positive ... papers, the purpose of our paper is to show the existence of twin or an arbitrary odd number of positive solutions to the BVP 1.1 , 1.2 The most important is that the authors would like to point out...
... blocks, half of which are coded with the distortion D0 , and half with the shaper distortion Ds Here we assume that all blocks of Description have the same expected distortion as blocks of Description ... Section PACKETIZATION AND TRANSMISSION The bitstream of the proposed video coder is packetized as follows A group of pictures (16 frames) is split into 3Dvolumes of size 16 × 16 × 16 One packet should ... Description If the size of such a block (one shaper volume and four residual volumes) is small, several blocks are packed into one packet The proposed coder uses DPCM prediction of DC coefficients in...