... apoptosis in testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction among germ ... pathways involved in testicular germcell apoptosis pertaining to specific treatments As previously shown [7], testicular cell apoptosis follows both extrinsic and intrinsic pathways The extrinsic ... resuspended in medium-199 containing 100 lgÆmL)1 streptomycin sulfate and 100 IUÆmL)1 penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess...
... resulting in a change from thymine to cytosine which led to a substitution in valine to alanine at position 166 Patient had a mutation at nucleotide number 332 resulting in a change from guanine ... followed by incubation in plastic flasks for hours The nonadherent cells were then washed away and the remaining adherent cells were cultured in 10% fetal bovine serum (FBS) DC medium containing 100 ... variability from day to day in staining or thawing The samples were tested in independent setups over days We have included internal controls in each experiment, one of those being a frozen leukapheresis...
... of malignant giant celltumors of bone or malignant foci inside benign giant celltumors has been described also in a small number of patients [24,25], and pulmonary metastases can be found in ... of giant celltumors As they are rich in stromal cells that express RANKL, a key mediator of osteoclast activation [14], increasing interest has been paid to monoclonal antibodies against RANKL, ... available Here we report our experience with intensitymodulated radiotherapy in the treatment of giant celltumors occurring outside the extremities in combination with a short review of the literature...
... receptors that contain intracellular tyrosine kinase domains The unique extracellular domain of these tyrosine kinase receptors (RTKs) is what permits them to be classified into distinct families (Figure ... cetuximab plus cisplatin/vinorelbine compared with cisplatin/vinorelbine alone as first-line therapyin EGFRexpressing advanced non-small -cell lung cancer Ann Oncol 2008 in press Pirker R, Szczesna ... of rapamycin) pathway leading to protein synthesis, and the PI3K-AKT (phosphatidylnositol-2 kinase Akt) pathway sustaining cell survival (Figure 2) In cancer cells, abnormal cell signaling through...
... (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each other (Figure 5) Within the ... bioluminescence imaging of intact cells HSV-TK-Luc expressing U87 glioma cells were transferred to a black microtiter plate in order to minimize light scattering, and MTT assay was performed in ... a protein known to be overexpressed in cancer cells [27], and (4) coelenterazine is much more expensive than D-Luciferin Iyer et al [28] examined noninvasive imaging using PET and BLI in CD-1...
... avail- Protein Name Cyclin dependant kinase Cell cycle regulating D-cyclin Cyclin E2 Proliferation of germ cells (POG) Cyclin D-dependant kinase inhibitors p27 Kip1 a Cdk inhibitor Cdk2 CyclinD2 Gcd/Pog ... spermatids) These intracellular signaling molecules in their absence cause impaired folliculogenesis resulting in a germcell deficiency within the gonads Effect on germcell development Sertoli cell specific ... coupling plays an indispensable role in early germcell development and in post-natal folliculogenesis Other signaling molecules implicating effects ingermcell development are ligands for tyrosine...
... injected in the gonad with 100 lL of saline solution containing dsRNA, or saline solution for the control After dsRNA injection, oysters were maintained in raceways in conditions allowing optimal ... Effects of in vivo oyvl-dsRNA injection on germcell development in oysters, month postinjection (A) Female control, injected with saline solution Oocytes are in vitellogenesis (B) Female injected ... specifically expressed ingerm cells and was thought to play a role in germline development [16,17] In this study, the oyster vasa-like gene was chosen to develop in vivo RNAi in the oyster, not...
... Ca2+-binding EF-hand family protein Plastid movement impaired (PMI2) Ca2+-binding EF-hand family protein Protein kinase Zinc-finger (C3HC4-type RING finger) family protein Zinc-finger (C3HC4-type RING ... Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis Plant Cell 19, 1898–1911 Podell S & Gribskov M (2004) Predicting N-terminal myristoylation sites in plant proteins ... synthesized in the wheat germ cell- free translation system Effect of the combination of amino acids at positions and on protein N-myristoylation in the wheat germ cell- free translation system Ser6 in...
... Freiburg, Germany) at a density of 107 cells/ ml for hr, and the adherent cells were incubated in CellGro DC medium containing 1000 IU/ml rhIL-4 and 1000 IU/ml rhGM-CSF (Cell Genix, Freiburg, Germany) ... surface as MHCII complexes, a crucial information for the clinical evaluation of patients involved in vaccination therapy trials Methods Dendritic cells Dendritic cells (DC) were prepared as described ... J, Steinman RM, Mellman I: Transport of peptide-MHC class II complexes in developing dendritic cells Science 2000, 288:522-527 10 Cella M, Engering A, Pinet V, Pieters J, Lanzavecchia A: Inflammatory...
... stem cells and Schwann Cells results in functional recovery in a rat spinal cord contusion injury model Biomed Environ Sci 2007, 20:242-249 Lindvall O, Kokaia Z, Martinez-Serrano A: Stem celltherapy ... S, Weidner N, Winkler J: Adult stem celltherapyin stroke Curr Opin Neurol 2005, 18:59-64 Li M, Decherchi P, Raisman G: Transplantation of Olfactory Ensheathing Cells into Spinal Cord Lesions ... until staining Immunohistochemistry staining Immunohistochemical staining of nestin and BrdU were performed to assess the property of NSC in cultures and track the migration and homing of allogeneic...
... MNCs in GCTs CD68, an intracellular glycoprotein, was expressed in monocyte lineage cells, including OPCs and OCs [20] Therefore, it is possible that the infiltrating MNCs and monocyte-like cells ... examine the pY-Flt-1 and pY-FAK levels in GCT samples, the staining intensity of each specimen was scored as follows: (weak staining; less than 10% of cells were positive), (intermediate staining; ... VEGF The VEGF levels in GCT-CM were determined using an ELISA kit from R&D Systems (Minneapolis, MN) Cell proliferation assay rOPCs cells seeded in culture plates were incubated in serum-free media...
... MNCs in GCTs CD68, an intracellular glycoprotein, was expressed in monocyte lineage cells, including OPCs and OCs [20] Therefore, it is possible that the infiltrating MNCs and monocyte-like cells ... examine the pY-Flt-1 and pY-FAK levels in GCT samples, the staining intensity of each specimen was scored as follows: (weak staining; less than 10% of cells were positive), (intermediate staining; ... VEGF The VEGF levels in GCT-CM were determined using an ELISA kit from R&D Systems (Minneapolis, MN) Cell proliferation assay rOPCs cells seeded in culture plates were incubated in serum-free media...
... indicating weak staining, the thick arrowhead indicating medium staining, and the tailed arrow indicating strong staining D: Staining with p53 antibody(original magnification ×250) The star (*) indicates ... Immunohistochemical Staining A: Hematoxylin-eosin stain (original magnification ×250) The star (*) indicates the malignant heterologous component of uterine MMMT B: Hematoxylin-eosin stain (original magnification ... Immunostaining results were scored on a semi-quantitative scale including staining intensity and percentage of positive cells The extent of immunostaining was divided into four categories according...
... significantly increased by SAHA and combination treatment as compared to HIT only in the two sarcoma cell lines - again in contrast to the osteoblast cell line Changes in the repair kinetics of DSB p53-independent ... observable 14 In contrast to our previous findings concerning the combination of XRT and SAHA in the three cell lines investigated [7], repair protein expression was not influenced by the combination ... which is in line to the mutated p53 Multiple pathways are involved in maintaining the genetic integrity of a cell after exposure to ionizing radiation A common cellular response to DNA damaging 15...
... staining Cells were dissociated and fixed in PBS containing 1% glutaraldehyde for h After washing in PBS, cells were stained with 200 μM Hoechst 33342, mounted on slides, and visualized using ... in BNCTtreated cancer cells should be clarified p21 binds to and inhibits the cyclin-dependent protein kinases that drive the cell cycle, and is responsible for G1 arrest [34-36] In SAS/neo cells, ... SAS/neo cells in the G0/G1 phase was 30%, and it increased to 39% at h after BNCT At 12 h, it decreased to 6%, and cells in the G2/M phase were increased to 34% Sub-G1 peaks, indicating apoptotic cells,...
... (combination of lamivudine, stavudine and efavirenz) followed by 1a (combinations of lamivudine, stavudine and nevirapine), 1d (combination zidovudine, lamivudine and efavirenz) and 1c (combination ... capsule using Indian ink following the standard procedure [14] Toxoplasmosis was diagnosed by detecting immunoglobulin G using Enzyme-linked immunosorbent assay in addition to its clinical features ... Lamivudine/Stavudine/Efavirenz Lamivudine/Stavudine/Nevirapine Zidovudine/Lamivudine and Efavirenz 21.8 22.1 0.92 Zidovudine/Lamivudine/Nevirapine 19.4 20.8 0.68 Single 28.8 31.9 0.42 Married 47.0...
... observed for the combinations of FWGE and 5-FU (6 out of cell lines) and to a lesser extend for irinotecan and oxaliplatin (2 out of cell lines) Drug interaction for the remaining cell lines was additive ... irinotecan or oxaliplatin was assessed in a panel of colon cancer cell lines The mode of drug interaction was analyzed by the method of Drewinko and the data summarized in table Overall, mainly ... cancer cell lines It’s interesting to note that the IC50-values of the human CRC cell lines included in this screen range close to the average IC50 Mueller et al Journal of Experimental & Clinical...
... Minami , Masaoka M, Matsuda H: Primary germcelltumorsin the mediastinum: a 50-year experience at a single Japanese institution Cancer 2003, 97(2):367-376 Droz JP, Horwich A: Extragonadal germ ... the final manuscript • Convenient online submission • Thorough peer review • No space constraints or color figure charges Competing interests The authors declare that they have no competing interests ... Droz JP, Horwich A: Extragonadal germcelltumors Comprehensive Textbook of Genitourinary Oncology New York: Lippincott Williams and WilkinsVogelzang NJ, Scardino PT, Shipley WU, Doffey DS , 2000...
... PE: 7-Aminoactinomycin D Allophycocyanin Bicinchoninic acid Bovine serum albumin 4',6-diamidino-2-phenylindole Dulbecco’s Modified Eagle Medium Erythropoietin Extracellular regulated kinase Fetal ... proliferation in certain cell types (including hematopoietic stem cells, progenitor cells, and mast cells) while highlighting the importance of genetic in vivo studies restricted to specific cell lineages ... protein Guanosine diphosphate Granulocyte-macrophage progenitor Guanosine triphosphate High proliferation potential cell Hematopoietic stem cell Hoechst Interleukin-3 Interleukin-6 Interleukin-13...
... whilst maintaining a tolerance to fuel impurities in the reformate feed Durability is one of the most critical remaining issues impeding successful commercialization of broad PEM fuel cell transportation ... three critical interfaces that must remain properly intermingled for optimum MEA performance: platinum/carbon interface (for electron transport and catalyst support); platinum/Nafion interface (for ... water effects are not included in the model, the cell voltage dose not exhibit an increasingly steep drop as the cell approaches its limiting current density This drop off in performance is clearly...