ms ms and sequential mass spectrometry msn
Báo cáo y học: "Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)" pps
... analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI -MS/ MS) Anal Chim Acta ... coupled with diode array detector and mass spectrometry (HPLCDAD-ESI -MS) and to assess the quality of two commercial mycelial AC products Methods Plant Powdered mycelium and an intact fruiting body ... coupled with an ion trap mass spectrometry detector was used The system was equipped with an electrospray ionization (ESI) source and an ion trap analyzer for UV and MS data acquisition A reverse...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... Direct organic solvent analysis demands some specific features and capabilities of the ICP -MS instrument, particularly in the sample introduction and plasma systems Details are given in the following ... chamber by self-aspiration The spray chamber and plasma torch are made of highpurity quartz and any seals in the sample introduction and drain systems are replaced with solvent resistant materials ... For quadrupole ICP -MS (ICP-QMS) the established and most effective method of reducing polyatomic interferences in high purity matrices is the use of the ShieldTorch System and cool plasma conditions...
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KHỐI PHỔ LC MS mass spectrometry LC MS
... T-Wave thứ hai: cho MS2 TOF: Lựa chọn V mode/ W mode GC -MS Tách GC Xác định MS Có thư viện phổ chuẩn Interface Mass spectrometer acts as a detector for GC LC -MS • Tách LC Xác định MS • Khó khăn: loại ... nhận số lượng chúng tỷ lệ với cường độ tín hiệu Máy MS tứ cực chập (Trip Quad) TSQ (Thermo) WS 2005 Waters Micromass Q-Tof Premier Waters Micromass Q-Tof Premier Phun: Z-spray T-Wave = ion optics ... phun điện ESI (Electrospray Ionization) LC -MS • Tách LC Xác định MS • Khó khăn: loại ảnh hưởng dung môi thành phần pha động; thư viện phổ • Để ghép nối LC MS: • giao diện ion hoá hoá học áp suất...
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Phương pháp phổ khối lượng (Mass Spectrometry - MS)
... Monitoring) MRM (Multiple Reaction Monitoring) Đối với MS tứ cực chập ba, máy đo khối phổ hai lần liên tiếp (MS/ MS), kỹ thuật ghi phổ MS/ MS có độ nhạy cao thường sử dụng SRM MRM + SRM: cô lập ... với thiết bị phân tích khối Giao diện kết nối GC vào MS tương đối đơn giản Sơ đồ khối cấu tạo thiết bị GC /MS- MS (kiểu tứ cực chập ba) − Với LC /MS, đầu LC dòng dung môi pha động đưa trực tiếp toàn ... cho MS; dễ dàng bảo dưỡng vận hành; thời gian lưu píc sắc kí đồng giảm thiểu tối đa doãng píc Với thiết bị LC /MS, phận ion hoá thiết kế phù hợp với giao diện kết nối LC với MS thường đặt phía MS...
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Water quality application of inductively coupled plasma mass spectrometry (ICP MS) part 2 determination of 62 elements
... plasma mass spectrometry (ICP -MS) for the determination of elements — Part 1: General guidelines and basic principles Terms and definitions For the purposes of this document, the terms and definitions ... coupled plasma mass spectrometry Blackie & Son Ltd., London (1989) [5] JARVIS, K.E GRAY, A.L and HOUK, R.S Handbook of inductively coupled plasma mass spectrometry, Blackie, Glasgow and London, ... 211-230 [2] ADAMS, F., GIJBELS, R and GRIEKEN, VAN R Chemical analysis, Volume 95 A series of monographs on analytical chemistry and its applications, inorganic mass spectrometry John Wiley and Sons,...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... UK) Peak lists were generated and processed using mascot distiller v2.1.1.0 (Matrix Science) Search parameters for peptide and MS ⁄ MS mass tolerance were ± 0.1 Da and ± 0.1 Da, respectively, with ... HLA-A1, SLC25A4 and TAPBP The results of the western blot analysis in the tumoral and normal tissues confirmed the LC -MS ⁄ MS results The expression 3032 levels of CLDN3, HLA-A1 and SLC25A4 were ... prior to MS analysis In our method, we decided to analyze the sample directly by RPLC -MS ⁄ MS three times to obtain a confident protein identification result Using the iTRAQ labeling mass spectrometry, ...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... 2.5, 8.0 and 11.5, and aliquots of each pH value were incubated at 37 °C, 50 °C and 70 °C, respectively From these, aliquots of lL were taken at 0, 1, 2, 4, and 24 h, and prepared for MS analysis ... production and purification of the enzyme [13] The molecular mass of each purified product, as measured by MALDI-TOF MS, was 11 947.87±2 for (Met1)-ONC (M23L) and 11 838.21±2 for rONC, which conforms well ... contribution of Met1, and Met23 substituted for Leu, Fig Comparison of the cyclization reaction as a function of temperature and pH (A), (B) and (C): (d), (s) and (.) correspond to pH 2.5, 8.0 and 11.5,...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... °CÆmin)1 and to 310 °C at 10 °CÆmin)1 ES -MS Negative-ion ES -MS and CID MS/ MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer Nitrogen was used as desolvation and nebuliser ... optimal fragmentation and, typically, 40–43 V was used for CID of [M-H]–, and 23–27 V for [M-H]2– A scan rate of 1.5 s per scan was used for both ES -MS and CID MS/ MS experiments, and the acquired ... pattern and sequence analysis of underivatized oligosaccharides by combined MS/ MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry J Am Soc Mass Spectrom...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... Rmsd bond lengths (A) Rmsd bond angles () Number of all atoms Number of protein atoms Number of waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms ... cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for proteomics and other biological ... using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry Mass Spectrom Rev 23,...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... (Asp) ) from TBDMS-Asp fragment 390 – and [2-3] of PYR (Ala) ) from TBDMS-Ala fragment 232 ) based on Eq (1) The relationship between the carbon atoms of Glu and these of OAA [2-4] and Ala [2-3] ... Modeling System (GAMS) environment (ÔGAMS Development Corporation, 1998, www.gams.com), a high-level modeling system for mathematical programming problems, and the weighted least-squares mass isotopomer ... of PEP, and thereby Phe, and these of PYR, and thereby Val, are expected to be at natural abundance Fig 8C, on the other Ó FEBS 2003 Systematic flux analysis using stable isotopes and MS (Eur...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... citrate and 0.1% formic acid) and spotted (0.5 lL) on a stainless steel MALDI target (Opti-TOF; Applied Biosystems) MALDI-TOF-TOF MS and MS ⁄ MS spectra were acquired with a MALDI-TOF ⁄ TOFÔ 4800 mass ... in 1% formic acid and stored at )20 °C until MS analysis High mass MALDI-TOF MS High -mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spectrometer ... and msx-3d [12]) with a mass tolerance of p.p.m We therefore used MALDITOF-TOF and ⁄ or nanoLC-Orbitrap tandem MS to further identify peptides from this list Twenty-five mono-linked peptides and...
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Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc
... ATLACPL and KS1 but not onto AT1, KR1, ACP1 or TE The loading module fragment also showed two peaks, LM1 and LM2 with mass increase of 67 and 137 Da (theoretical mass increase of 70 and 140 Da, ... specificity and organization of type I PKSs The methodology is based on accessible and simple mass spectrometry equipment rather than advanced FT-ICR -MS Nevertheless, the technology can achieve mass ... Limited proteolysis and MS of modular PKSs Fig Deconvoluted mass spectra of loading didomain ATL-ACPL released from DKS by limited proteolysis (A) unliganded loading module; (B) and (C), loading...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... threshold 441 Present and < Mass 443 High -mass resolution 442 Base peak or >50% of Mass 198 High -mass resolution and sensitivity 443 15-24% of Mass 442 High -mass resolution and isotope ratio All ... peak Low -mass sensitivity 68
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MASS SPECTROMETRY IN DRUG METABOLISM AND DISPOSITION docx
... Lee, and Guodong Chen Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... hybrid mass analyzers for MS/ MS studies, and ancillary techniques such as ion mobility spectrometry, have added new dimensions to the mass spectrometry experiment, while the advent of high mass ... Accelerator Mass Spectrometry 525 John S Vogel, Peter Lohstroh, Brad Keck, and Stephen R Dueker 17 Standard-Free Estimation of Metabolite Levels Using Nanospray Mass Spectrometry: Current Statutes and...
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CHARACTERIZATION OF IMPURITIES AND DEGRADANTS USING MASS SPECTROMETRY pot
... Screening HPLC -MS and Infusion Orbitrap MS 4.9 Determining Substructures: Orbitrap Tandem Mass Spectrometry (MSn) 4.10 Multianalyzer (Hybridized) System: The Linear Ion Trap/Orbitrap for MS/ MS and Higher-Order ... TANDEM MASS SPECTROMETRY Tandem MS is the practice of using multiple stages of ion manipulations, including m/z isolation and fragmentation Tandem MS is also referred to as MS/ MS” for two TANDEM ... such as liquid chromatography mass spectrometry (LC -MS) profiling and liquid chromatography–tandem mass spectrometry (LC -MS/ MS) substructural techniques has become a standard analytical platform...
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Tandem Mass Spectrometry – Applications and Principles Edited by Jeevan K. Prasain docx
... instruments are also used to perform tandem mass spectrometry in space (Hoffmann and Stroobant 2007) Tandem Mass Spectrometry – Applications and Principles Higher order MSn spectra can be acquired by ... reaction enthalpies, proton and ion affinities, gas-phase acidities, and so on 4 Tandem Mass Spectrometry – Applications and Principles Mass spectrometry is extremely versatile and several areas of ... Introduction 1.1 Mass spectrometry brief history and recent developments The first and most important question to be asked: What is mass spectrometry? The basic principle of mass spectrometry (MS) is to...
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mass spectrometry of proteins and peptides
... of tandem mass spectrometry (MS/ MS /MS or MS3 ) using nanoelectrospray on an ion trap (17) Thus, the first stage of tandem mass spectrometry produces the series of doubly charged y-type ions, and ... Winston, S., and Hauer, C R (1986) Protein sequencing by tandem mass spectrometry Proc Natl Acad Sci USA 83, 6233–8238 Yost, R A and Boyd, R K (1990) Tandem mass spectrometry: quadrupole and hybrid ... conventional scan (m/z 400–1600) and for the three MS/ MS experiments On the LCQ instrument, the mass range for MS/ MS is calculated based on a +2 charge state Tandem mass spectra are searched against...
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protein and peptide analysis by mass spectrometry
... Resonance Mass Spectrometry VCH, Wemheim, Germany 15 Busch, K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry /Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry ... Tandemmassspectrometryor MS/ MS (sometimeswritten MS2 ) is an important technique that is proving to be increasingly useful in many areasof analysis (15) Most MS/ MS instruments consist of two massanalyzers ... mstrument, and can open up new, analytically useful, fragmentation routes Ion-trap and FTMS mstruments are suitable for MS/ MS experiments without being combined with other mstrumentation and can...
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Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot
... of 3.5 kV The MS/ MS data was recorded using a 0.5 sec MS survey scan and 2.5 sec MS/ MS scans on the three most abundant ions found in the survey scan The CID energy was between 25 and 65 eV depending ... analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis of the mass spectrometry data DEH and CSM coordinated the research efforts and edited the ... membrane- and core-enriched fractions were directly digested with trypsin, and analyzed using LC-ESI-Q-TOF MS This methodology resulted in 319 tandem mass spectra that matched 202 total peptides, and...
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