mota b proton channel complex of the proton driven flagellar motor of salmonella enterica
Báo cáo khoa học: "Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles" ppsx
... nim rof Co56 ta dna nim rof Co35 ,nim rof Co49 ta selcyc 03 yb dewollof saw nim rof Co59 ta elcyc ,sremirp CIRE eht roF nim rof Co56 ta dna nim rof Co25 ,nim rof Co49 ta selcyc 03 ,nim rof Co59 ... siditiretnE S bk 75-45 a fo ecneserp eht detroper evah ]2[ la te relhciB dnab ezis bk51 a derobrah setalosi siditiretnE S llA fo mumixam ot morf erew snrettap eseht rof srebmun dnab ehT bk12 ot 9.1 ... noissucsiD nekcihc morf setalosi ni ylno dnuof erew bk 51 naht rellams fo sezis dnab eht tub ,detset setalosi lla ni detceted saw ezis bk 51 fo dnab A dnuof erew ,bk 12 ot 9.1 morf gnignar ezis ralucelom...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... reduction of the double bond by FMN, the N10-N5-C5 angle value of 92° (Fig 7B) is distinct form the range of angles (125° to 170°) observed in flavoenzyme-substrate complexes [31] Any putative motion of ... productive binding of steroid substrates: in the first, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive ... both residues in the binding of substrates and inhibitors (Table 1; Fig 2) Anaerobic stoppedflow studies of FMN reduction by NADPH in H181A and H184 enzymes indicated reduction of the FMN, Table...
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Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf
... This kind of protein is exemplified by the Thermus thermophilus protein, PDB entry 2B3 B [28] The mode of binding the monosaccharide is completely different in terms of orientation of the sugar, ... [27] By presenting the sugar first, recognition by the permease can be largely independent of the presence or absence of the glyceryl moiety Comparison with available sequences The presence of the ... Fig 5B, illustrating the ‘fan’ of A B related conformations observed The GGal complex is the most closed structure found to date, perhaps because of the significantly larger number of hydrogen bonds...
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... al to the 500 kDa bc1 sub -complex Therefore, the presence of both Qcr9p and Bcs1p is required for the insertion of ISP into the bc1 sub -complex, but the presence of only one of these two subunits ... TX-100 B 150 500 kDa bc1 sub -complex (%) To investigate the stability of the association between the bc1 subunits in the 500 kDa bc1 sub -complex, Triton X-100 was used for the solubilization of the ... putative sub-complexes bind to each other during the assembly of the bc1 complex is also unknown Furthermore, as in the case of the biogenesis of other multi-subunit complexes of the mitochondrial...
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Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt
... KCNQ1–KCNE1 channels Diversity of KCNE4 expression KCNQ1 + KCNE1 T NB B 30 kDa 25 IB:FLAG 15 35 kDa B IB:GAPDH KCNQ1 + KCNE4 T NB B 50 kDa 30 25 IB:HA 35 kDa C IB:GAPDH KCNQ1 + KCNE1 + KCNE4 T NB B 75 ... accessory subunits Conceivably, the variety of channel complexes can be expanded further by mechanisms combining pore-forming subunits with multiple different types of accessory subunits In considering ... to block the internal pore of KCNQ1 The C-terminus of KCNE4 might also stabilize the channel in another non-conducting state There have been no investigations into the structural determinants of...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... important in the brain, therefore the role of copper binding or loss of its binding could be related to specific cerebellar function(s) of stefin B [18], which remains to be seen by more in vivo ... EMBO J 20, 4629– 4633 12 Stubbs MT, Laber B, Bode W, Huber R, Jerala R, ˚ ˇ ˇ Lenarcic B & Turk V (1990) The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the ... the maximal value of ThT fluorescence intensity was taken as 100% From these, for each reading of ThT fluorescence the percentage of inhibition of the fibril growth was obtained The percentage of...
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Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf
... 386 of PhKc from rabbit muscle Identities are shown by the blue boxes with white text, while blue text and green boxes indicate regions of sequence similarity Numbering is taken from the rabbit ... conformational flexibility in the two EF hand motifs of each CaM domain The presence of a relatively large number of Ca2+⁄ CaM ⁄ peptide complexes in the PDB opens up the possibility of using these structures ... binding but also in the relative orientation of the two CaM domains These differences are facilitated by the flexibility of the linker between the two domains as well as a certain degree of conformational...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... appears as a double band in unboiled samples (lanes A1 and B1 ) Identification of the genes encoding the subunits of the Hme complex and sequence analysis The N-terminal sequences of the four polypeptides ... containing only minor amounts of the 16-kDa c-type cytochrome The Table Features of the subunits of the Hme complex from A fulgidus Data are either derived from the analysis of the sequence (calculated ... cytochrome and the hydrophilic catalytic subunit [7]; the subunit structure of the Hme complex isolated from A fulgidus is considerably more complex The analysis of the sequence of the gene cluster...
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Báo cáo khoa học: Estrogen-related receptor a and PGC-1-related coactivator constitute a novel complex mediating the biogenesis of functional mitochondria potx
... there were four times as many FTC-133 cells as RO 82 W-1 cells The level of inhibition was probably related to the different proliferative statuses of the cells Nevertheless, the inhibition of ... the ERRa– PRC complex is involved in the control of the early phase of the cell cycle This is in accordance with the role played by PRC and ERRa in the transition from the G1 to the S phase of ... Chalfont, Buckinghamshire, UK) by electroblotting The membranes were incubated in 5% nonfat milk in TBSTween (Tris-buffered saline with 0.1% Tween-20) The membranes were incubated with dilutions of the...
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Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx
... BN-PAGE, probably because they were below the level of detection The identified complexes resemble subcomplexes of the importomer of the peroxisomal protein import machinery originally described ... interaction of the membrane-bound AAA complex and the importomer Therefore, the protein complexes isolated with protein A fusions of Pex1p, Pex6p and Pex15p were tested for the presence of peroxins of the ... data not shown), thereby confirming the specifity of the isolation Taken together, these data indicate a close association of the membrane-bound AAA complex and the importomer The findings that...
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Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... respectively The distances between the sugars and the aromatic rings of ˚ the two residues are 4 A The mode of acarbose binding to the active site readily explains the exoglucanase activity Raw starch binding ... the resolution of the native Glu structure, but has in addition revealed the presence of a second acarbose (substrate analogue) ˚ binding site on the surface of the enzyme, 25 A remote from the ... active site and the other on the sur˚ face of the enzyme about 25 A away, Fig The active site acarbose fits tightly into the pocket (Fig 3) and the electron density for all the acarbose atoms is...
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Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf
... wash Bound proteins were then eluted by incubating the beads with 20 lL of 10 mM Fig Binding of B5 6a to the A subunit of PP2A [35S]Methioninelabeled proteins generated in vitro were incubated ... included in the pro®les The binding of the two conserved regions from B and PR72 to GST-A To test whether these two conserved regions of B subunits found in B/ B55/CDC55 and PR72 /B ¢ family members indeed ... Substitution of certain amino acids in the intrarepeat loops abrogates the binding of some B subunits but not others [13,28] The results here de®ne two distinct PP2A binding domains in the B subunits...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... 29 3N9K a BGC1 is the glucose residue from the nonreducing end of laminaripentose (L5) bound to the exterior of the protein b BGC2 is the weakly bound glucose residue of L5 bound in the Phe-Phe ... Hydrogen bonds formed between the glucose moiety and the protein backbone A bridging water molecule makes two additional hydrogen bonds to the backbone (D) Space filling model of the bound glucose ... solved the crystal structure of this GH5 enzyme in the presence of two different mechanismbased inhibitors, thereby revealing the close network of interactions that hold the terminal glucose of the...
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Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
... assumed to be an assembled Thermoplasma complex of 50S DLS is better able to deal with the polydispersity in the sample of assembled complex Analysis of the autocorrelation curve using the volume ... with the substrate specificity observed for the recombinant E1 enzyme in the absence of the other complex components [14] The relative activities are given in Table 1, along with those for the ... the E coli OGDHC and of the Bacillus subtilis BCOADHC (Fig 1) However, in addition to the identity of the neighbouring residues, it is the exact positioning of the lysine in the lipoyl domain that...
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Báo cáo khoa học: Hemitoxin, the first potassium channel toxin from the venom of the Iranian scorpion Hemiscorpius lepturus ppt
... lM unlabeled aDTX and was subtracted from all data points Total binding (B0 ) was determined in the absence of ligand Specific binding (%) was obtained by calculating 100 · (B – NS) ⁄ B0 The data ... model of HTX Backbone ribbon representation of the models of HTX: HTX mod1 was obtained using atomic coordinates of both MTX and Pi4 as templates HTX mod2 was obtained using atomic coordinates of ... the binding to the voltage-gated K+ channels in a number of toxins [16,31,34,35] It was established that Kv channel toxins exerted their activity through the solvent-exposed face of their b- sheet...
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Báo cáo khoa học: Concerted mutation of Phe residues belonging to the b-dystroglycan ectodomain strongly inhibits the interaction with a-dystroglycan in vitro pot
... polyclonal antibody directed against the C-terminal domain of a-DG on intact 293-Ebna cells (B) Detection of b- DG was carried out using b- DG antibody in permeabilized 293-Ebna cells Both subunits of wild-type ... binding between the two DG subunits in vitro, has no evident effect on the plasmalemmal targeting of b- DG (Fig 5B) The correct membrane targeting of DG was also confirmed by the immunodetection of the ... which greatly reduces the affinity of b- DG for the a-subunit in vitro, did not influence the localization of the two DG subunits (Fig 5A ,B) In order to detect any effect of the double mutation Phe692...
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Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
... N-terminus of helix I of the domain and the three residues in loop L2 contributing to the E3-binding site Backbone dynamics of PSBD The region (Met132 to Ser134) before the start of helix I of the PSBD ... differences between the backbone resonances of the uncomplexed PSBD and PSBD bound to E3int indicate that several amino acids near the N-terminus of helix I of the PSBD are at or near the E3-binding ... representing the PSBD of the dihydrosuccinyltransferase chain of the 2-oxoglutarate dehydrogenase complex of E coli [12] and of the dihydroacetyltransferase chain of the PDH complex of B stearothermophilus...
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Báo cáo khoa học: PA700, the regulatory complex of the 26S proteasome, interferes with a-synuclein assembly pptx
... promote the assembly of the 26S proteasome from the PA700 complex and 20S proteasome; (b) the opening of the channel leading to the 20S proteasome; and (c) the binding and unfolding of substrate ... superimposable to that of a-synucleins in the absence of PA700 at 140 lm If PA700 binds monomeric a-synuclein, the assembly kinetics in the absence or the presence of PA700 would be superimposable as the ... and B) In the elongation phase (time h), semiflexible, unbranched fibrils are observed They coexist with the globular and the short curved oligomers observed at the earlier stages of assembly (Fig...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
... combed as described below For the study of the interaction between C1r and C1q bound to combed T4 DNA, 150 lL of 6.5 pM T4 DNA were preincubated with lL of lM unlabeled C1q for 45 min, then lL of ... association between the A, B and C chains constituting the six ABC heterotrimeric triple helices of C1q The cationic sequence 14–26 of the A chain, as well as the sequence responsible for the kink of the ... in the absence of C1q (data not shown) Therefore the binding of C1r is a direct consequence of the presence of C1q on DNA strands Ó FEBS 2003 4718 B Tissot et al (Eur J Biochem 270) Altogether...
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Báo cáo khoa học: The complex of the insect LDL receptor homolog, lipophorin receptor, LpR, and its lipoprotein ligand does not dissociate under endosomal conditions pot
... that the stability of the complex is imparted by LA-1–7, which were shown to comprise the binding site for HDLp The data accumulated imply that the stability of the complex is engendered by the ... Quantification of the number of cells in population revealed that the number of cells that bound ligand was 91.5 ± 6.3% of the number that bound antibody, indicating that binding of HDLp was proportional ... endosomal pH was able to disrupt the complex, the issue was addressed of whether LpR binds HDLp in a different manner than other LDLR family members bind their ligands Therefore, the ability of receptor-associated...
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