... for these molecular and functional propertiesof the triple mutant, and thereby gain more knowledge about the zymogenicity determinants of FVIIa, mutants with substitutions at one or two of positions ... rate of hydrolysis of peptides of various lengths (from ể FEBS 2002 5954 E Persson and O H Olsen (Eur J Biochem 269) P4-P1Â to P4-P7Â of FX) by V158D/E296V/M298Q-FVIIa as compared with that of ... different from that of wild-type FVIIa (not shown) The amidolytic activity of free FVIIa has been shown to be at least about 10 times higher in the presence of mM Ca2+ than in the absence of the metal...
... Alignment of the deduced amino acid sequence of lac1 with deduced amino acid sequences of other fungal laccases showed highest overall identity with laccase (BAB84354) from Lentinula edodes (58%), laccase ... XYL [5] Higher levels oflaccase mRNA were also reported in cultures of three white-rot fungi supplemented with aromatic compounds [47] Transcription of only one of two laccase genes in T versicolor ... oxidase of Cryptococcus neoformans: identification as a laccase J Bacteriol 176, 656–664 39 Zhao, J & Kwan, H.S (1999) Characterization, molecular cloning, and differential expression analysis of laccase...
... lg of platelet protein and 0.5 lg of GST and GST±Rab proteins; (B) an [a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lg of GST and 0.5 lg of ... form of Rab32 (see Discussion) Fig Puri®cation and propertiesof wild-type and mutant Rab31 and Rab32 expressed as GST±fusion proteins Samples of platelet particulate fraction protein and of puri®ed ... (Fig 4) To determine the amounts of these Rab proteins in platelets, immunoblots of 10±20 lg of platelet protein were compared with those of standard amounts of the recombinant Rab proteins (0.5±20...
... 10 20 50 500 500 500 25 25 – – – – 2392 000 000 000 000 000 000 000 000 000 of 0.5 fs of 1.0 fs of 2.0 fs of 2.0 fs of 2.0 fs FEBS Journal 274 (2007) 2385–2395 ª 2007 The Authors Journal compilation ... uncertainties of peak intensities were evaluated as the SD of the spectral noise measured in a region free of crosspeaks The heteronuclear NOE values were determined from the ratio of peak volumes of spectra ... Ciccotti G & Berendsen HJC (1977) Numerical integration of the Cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes J Comput Phys 23, 327–341 39 Berendsen HJC,...
... properties to Program Offices (e.g., Office of Water, Office of Solid Waste and Emergency Response, Office of Prevention, Pesticides and Toxic Substances) and Regional Offices Also, SPARC has been used ... 3.4.5 Conclusion PHYSICAL PROPERTIES 4.1 Estimation of Physical Properties 4.2 Physical Properties Computational Approach 4.3 SPARC Molecular Descriptors 4.3.1 Average Molecular Polarizability ... bimolecular union to form a transition state or distinct product The behavior of chemicals depends on the differences in electronic propertiesof the initial state of the system and the state of...
... investigation of the inuence of the heme orientation on functional propertiesof myoglobin Biochim Biophys Acta 1388, 349362 Emerson, S.D & La Mar., G.N (1990) NMR determination of the orientation of the ... globins A reliable indicator of the dynamic stability of distal heme pockets in globins is the rate of reorientation of the aromatic rings in the heme pocket [37,53] The rate of reorientation can be ... the tilt of the major magnetic axis, z, away from the heme normal (z axis), a is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and...
... moiety of the ATP molecule, but that it is merely within reach of the highly reactive azido group of 8-azido-ATP In the crystal structure of the Ca2+-ATPase, Lys492, the equivalent of Lys480 of the ... the presence of both subunits The a subunit, which is referred to as the catalytic subunit, has a relative molecular mass of 100–113 kDa, depending on the presence of different isoforms: a1, a2, ... is highly glycosylated and has a relative molecular mass of about 60 kDa The mass of the protein moiety of this subunit is 36–38 kDa, depending on the isoforms b1, b2, or b3 The b subunit crosses...
... advantages: Properties can be calculated from only four basic molecularproperties These are the molecular weight, van der Waals volume, length of the repeat unit, and the Tgof the polymer Since these properties ... value of the storage modulus G' ob- 2oooo r / Mf,gdmole Figure 12 Molecular weight at zero flexural strength ( M , ) vs entanglement molecular weight ESTIMATION OF MECHANICAL PROPERTIESOF POLYMERS ... discussion of these phenomena The molecular weight dependence of a polymer is strongly related to the entanglement molecular weight Above 2Me, the viscosity of the melt increases as the 3.4 power of...
... organization of a system, can be and of time function of redundancy (see Annex) Evolution of the organization as a expressed as a be calculated function of time can thus different types of showing ... Cy-hydroxyls of a monomeric propane chains (Fig 1C5to Molecular associations and cell wall lignification Formation ofmolecular associations with other cell wall components is the third main feature of ... 1976), as in the case of secondary metabolism (Terashima and Fukushima, 1988) these authors: in Fagus, the lignin moiety of LCC would consist of a small number of extremely large molecular fractions,...
... propertiesof food proteins: effects of reduction of disulfide bonds on film properties and foam stability of glycinin Journal of Food Science 1987, 56, 242-258 27) Kinsella, J E Functional properties ... type of coagulants will form gels of different physicochemical properties, thus the effects of other coagulants should also be studied Chapter of the study involved the study of the effect of chloride-type ... and emulsifying propertiesof the protein system The chapter of the study involved the study of the foaming and emulsifying propertiesof 2S soy protein in relation to other molecular protein...
... rotational free energy of the system of non-interaction rotators and is one of angles determining the position of symmetric axe ofmolecular field for the coordinate system of crystal In self-consistent ... constant volume of the molecular crystals of type N2 is studied by combining the SMM and the SCFM This paper represents further investigations of anharmonic effects of lattice vibrations and molecular ... energy of crystal is that of N harmonic oscillators: Ψvib ≈ 3N u0 + θ x + ln(1 − e−2x ) , ; u0 = ϕi0 (2) i Using SCFM, only taking account ofmolecular rotation, the rotational free energy of crystals...
... Candidate Molecular Wires and their properties ………………………………… 10 1.3.1 Organic molecular wires………………………………………………………10 1.3.2 Organometallic molecular wires……………………………………………….12 1.4 Self assembly ofmolecular ... or taken into account in the design ofmolecular devices It is also interesting to explore the possibility of controlling the electronic propertiesof the molecular devices using electric or ... simulations and experimental results.93 1.8 The scope of my work The scope of my project includes investigating propertiesof a variety ofmolecular wires on different platforms In my first chapter,...
... and Optical PropertiesofMolecular Complexes 203 Interaction occurs generally by transfer of a lone electron pair from an elec tron donor or base into an unfilled molecular orbital of an electron ... Sec 4.4 of the present book (A.V.) Chemical and Optical PropertiesofMolecular Complexes 211 shaping factors are the mechanical and electrical coupling of the internal and external modes of the ... Optical PropertiesofMolecular Complexes 221 phonon transition opening the possibility of vibrational selectivity study The first works of infrared conversion in matrices have focussed upon unimolecular...
... operations Chapter 7- Propertiesof Convolution 127 2.0 a Original signal Amplitude 1.0 0.0 -1.0 FIGURE 7-3 Example of calculus-like operations The signal in (b) is the first difference of the signal ... curve of the form: sin(x)/(x) , is used to separate one band of frequencies from another c Sinc 0.3 Amplitude Amplitude 0.3 0.2 0.1 0.0 -0.1 -20 -15 -10 Sample number Chapter 7- Propertiesof Convolution ... affect any of the output signal values As shown by the examples in Fig 7-6, the impulse response of a causal system must have a value of zero for all negative numbered samples Think of this from...
... Assistant Professor of Medicine and Staff Physician at the Department of Gastroenterology and Hepatology, University Hospital Freiburg, Germany Since 2004 he is the head of the Division of Gastroenterology ... independently of the pre-initiation factors necessary for cap-dependent translation The three-dimensional structure of the HCV IRES bound to the 40S ribosomal subunit illustrates the molecular basis of ... the understanding of the molecular mechanisms of this enzyme could enable a specific inhibition as a novel antiviral strategy [47, 48] 5.4.3 NS4B Due to its very hydrophobic properties, NS4B belongs...
... is the appearance of serum markers of HBV infection that establishes the diagnosis of the disease Of these, HBsAg is considered to be the sentinel marker for the confirmation of acute infection ... necessary should the rate of emergence of resistance and the impact of such mutations turn out to be clinically relevant for therapy management Molecular Mechanisms of Drug Resistance HBV is ... technology, molecular beacons) that decrease the number of handling steps, reduce contamination, and increase throughput and the accuracy of quantification will further enhance the utility of these...
... summary of the independent parameters the modified foaming apparatus FOAMING PROPER TIES used far experi- Nature Fixed Diameter Porosity Volume Volume Mass ofof sparging disc of sparging disc of ... = Tw Did = = percent of gas initially in 70 ml of foam (gas Flow Rate) - ml/min of nitrogen time to fill the column with foam volume of liquid in the foam initially volume of liquid drained from ... characteristics of protein i.e the foaming capacity of protein and the stability and strength of the foam Foaming ability i.e Gi denotes the amount of gas retained in the foam reflecting the potential of...
... University of Bari in 1979 University Researcher at the of Engines and Energy Institute of the Faculty of Engineering at the University of Bari from 1983 to 1987 Associate professor of Technical ... measure of conductance The analyses, done in the laboratory of Technical Physics of the Polytechnic of Bari, were performed on some samples of calcarenite sandstone and calcareous stone of Fasano ... typologies of building wall: a wall in blocks of calcarenite sandstone assembled with mortar, typical of the constructions of "Sassi of Matera," and a masonry made with dry calcareous stones of Fasano,...
... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension ... Increasing time of heatsetting had effect on tensile propertiesof PLA at varying times of 30s to 90s at temperature of 130°C Optimum effect on tensile properties occurred at times of 30 – 45s PLA ... [PLA] Figure Mean [Ā] of effect of time of heatsetting on the tensile extension EM [%] of Ingeo™ Poly (lactic acid) 3.2 Linearity of load extension curve LT [-] The linearity of load extension curve...