molecular and signaling pathways that modulate mesenchymal stem cell self renewal

Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2

Ngày tải lên : 11/09/2015, 16:02

... differential expression patterns between ES cells and differentiated cells and these may be prime candidates to examine further for a role in ES cell self renewal Of primary interest are genes whose ... specifically that the enzymes and cellular machinery required to process Lefty2 are present in ES cells and assures us that Lefty2 processing is indeed active in ES cells and that mature Lefty2 proteins ... us that Nodal signaling may be active in ES cells and this further render such a hypothesis plausible It is a well established fact that SMAD2 is an intracellular mediator of Nodal signaling, and...

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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3

Ngày tải lên : 11/09/2015, 16:02

... embryonic stem cell fate Also, this thesis extends the current understanding of ES cell self renewal mechanism beyond the established roles of LIF/STAT3, BMP and WNT pathways and that of the core ... maintenance of ES cell self- renewal To achieve this aim, a candidate gene approach was adopted and 70 candidates were shortlisted An RNAi screen in mouse ES cells (E14) using Oct4 and Nanog promoter ... mechanics governing ES cell self- renewal Much is left to be learnt The primary aim of this project hence serves to identify and delineate the roles of additional factors and pathways that are important...

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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4

Ngày tải lên : 11/09/2015, 16:02

... 4 Materials and Methods 4.1 Cell culture 4.1.1 Routine cell line maintenance All cell cultures were maintained at 37 °C with 5% CO2 and were fed daily Feeder-free E14 Mouse ES cells (ATCC, Manassas, ... transfection 4.5.1 Mouse ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For ... medium, few cells remained in the untransfected control wells indicating that selection was effective The surviving cells were trypsinized and resuspended in ES cell medium 300 or 500 cells were...

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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5

Ngày tải lên : 11/09/2015, 16:02

... M., Andrews, P W., Itskovitz-Eldor, J., and Daley, G Q (2004) LIF/STAT3 signaling fails to maintain self- renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and ... Yuan, X., and Sharkis, S J (2006) Activin A maintains self- renewal and regulates fibroblast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells Stem Cells 24, ... Moore, H D., and Andrews, P W (2004) Specific knockdown of Oct4 and beta2microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells Stem Cells 22, 659-668...

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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency

Ngày tải lên : 11/09/2015, 16:02

... renewal and cell fate determination of ES cells 1.2 Molecular basis underlying ES cell self renewal Despite the importance of stem cell self renewal, we are only beginning to understand how it ... indeed plays a role in self renewal and cell fate decisions of pluripotent cell populations in vivo and also that of embryonic stem cells in vitro An in-depth mechanistic understanding of how Nodal ... ES cell self renewal and pluripotency revolves around the roles of LIF/STAT3, BMP and WNT signaling pathways, and that of the core transcriptional regulatory circuit governed by OCT4, SOX2 and...

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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency

Ngày tải lên : 11/09/2015, 16:03

... ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed with ES transcription factor like Nanog during mouse preimplantation development Oct4 and ... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... Sox 2and Nanog target in human ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells...

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Báo cáo khoa học: A biophysical view of the interplay between mechanical forces and signaling pathways during transendothelial cell migration doc

Ngày tải lên : 22/03/2014, 21:20

... transmigration Cell transmigration is also involved in processes such as cancer cell metastasis and stem cell homing, and although the steps of cancer cell transmigration are similar to those for immune cells, ... PECAM-1 [44] These results suggest that cell cell junctions both sense and transmit local forces Cell cell contact has been shown to both inhibit and stimulate cell proliferation, in different ... regulate cell cell contact For example, a recent study by Gray et al [45] has demonstrated that EC proliferation is biphasic with regard to degree of cell cell contact In this study, cell cell contact...

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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc

Ngày tải lên : 30/03/2014, 04:20

... vivo and in vitro, to pinpoint the efﬁciency and the effectiveness of MK591 Experimental procedures Cells and cell cultures Effects of SEB on human PBMCs vitamin solution (Gibco BRL, Grand Island, ... ⁄ reduce aberrant signaling and cellular activities that may ultimately lead to SEB-induced lethal shock Our goal was to evaluate multiple signaling cascades induced by SEB and target pathway ... major signaling systems efﬁciently utilized by cells to transmit and integrate a plethora of intracellular activities [6] Although the well regulated signal transduction is crucial for typical cell...

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Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt

Ngày tải lên : 18/06/2014, 19:20

... marrow-derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic origins, the ethical issue regarding the source and safety of allo- and xeno-grafting ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note ... organ dysfunction after stem cell therapy [12,13,16,20,28] In the present study, we found that both CM-Dil-positive cells and cells positive for endothelial markers (i.e CD31 and vWF), were abundantly...

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BREAST CANCER – CARCINOGENESIS, CELL GROWTH AND SIGNALING PATHWAYS ppt

Ngày tải lên : 27/06/2014, 17:20

... epithelial cell ADAM-17 to shed AREG which then, is necessary to stimulate EGFR signaling in the stromal fibroblasts 2.1.2 Breast epithelial stem cell and ErbB signaling Potential mammary gland stem and ... the ligand during cardiac development (97) 14 Breast Cancer – Carcinogenesis, Cell Growth and Signaling Pathways In the context of cellular behaviors, it appears that the EGFR signaling system ... shedding for the ligand to be released and available to bind receptors that may be on the same cell (autocrine signaling) , or on neighboring cells (paracrine signaling) (5) The proteases that mediate...

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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Ngày tải lên : 10/08/2014, 05:21

... converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues using ... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... factors, such as Oct4 and Nanog, normally expressed in early embryos and ESCs, inhibit tissue-specific genes and enhance self- renewal and pluripotency [24] To evaluate whether loss of stem- like properties...

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Báo cáo y học: " Human T lymphotropic virus type-1 p30II alters cellular gene expression to selectively enhance signaling pathways that activate T lymphocytes" doc

Ngày tải lên : 13/08/2014, 13:20

... such as apoptosis, cell cycle, cell adhesion, transcription/translation factors and T cell activation or cell signaling In all the categories, p30II was an overall repressor of cellular gene expression, ... [11,12] and cytotoxic T cells [13] that recognize recombinant proteins or peptides of the pX ORF I and II proteins are present in HTLV-1 infected patients and asymptomatic carriers Using molecular ... regulator of gene expression and identified several novel and important alterations in gene expression profiles, unique to cell cycle regulation, apoptosis and T cell signaling/ activation In addition,...

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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)

Ngày tải lên : 09/09/2015, 11:21

... endothelial cells and parenchymal cells after APAP overdose before cell injury [29] Both hydrogen peroxide and peroxynitrite decomposition products, OH and NO2 radicals can initiate LPO and lipid and ... Lai, W Wong, Y Y Dan, S K Lim and H K Ho, Mesenchymal stem cell- derived exosomes promote hepatic regeneration in drug-induced liver injury models, Stem Cell Research and Therapy, 5(3):76 (2014) ... cytotoxicity and cellular uptake of -TP, -T3, T3 and -T3 in TAMH hepatocytes 110 Figure 28 Concurrent effects of -TP, -T3, -T3 and -T3 in cell viability after APAP- and H2O2-induced...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... 2009;Human mesenchymal stem cells express vascular cell phenotypes upon interaction with endothelial cell matrix J Cell Biochem (4):714-722 103 Lozito TP, Taboas JM, Kuo CK, et al 2009 ;Mesenchymal stem ... Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, self- renewal and differentiation Arthritis Res Ther (1):204 ... 123 Beresford JN 1989;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991 ;Mesenchymal stem cells J Orthop Res (5):641650 125...

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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Ngày tải lên : 18/02/2014, 16:20

... catabolism and insulin resistance and sensitizes cells to apoptotic stimuli The inﬂuence of cell volume on cell function requires structures that register volume changes (‘osmosensing’) and trigger signaling ... relevance The understanding of how cell swelling integrates into the cell cycle machinery and how cell shrinkage sensitizes cells to apoptotic stimuli requires further scientiﬁc effort Cell hydration ... trigger signaling pathways towards effector sites (‘osmosignaling’) Anisoosmotically exposed cells and tissues were frequently used as a model in order to study osmosensing and osmosignaling By this...

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NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) ppt

Ngày tải lên : 20/03/2014, 09:21

... Therapeutic potential of mesenchymal stem cells producing interferonalpha in a mouse melanoma lung metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy: ... show that the mesenchymal stem cells injected into mice at the dose of 107 cells/ml can inhibit the proliferation of cancer cells The number at tumors in mice injected with mysenchymal stem cells ... cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING MESENCHYMAL (MESENCHYMAL STEM CELL - MSC) IN THE TREATMENT OF CANCER AGENCY - RECTUM RATS...

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báo cáo hóa học: " Function, Adjustment, Quality of Life and Symptoms (FAQS) in Allogeneic Hematopoietic Stem Cell " potx

Ngày tải lên : 20/06/2014, 15:20

... V, Lauson J: Development, feasibility and compliance of a web-based system for very frequent QOL and symptom home self- assessment after hematopoietic stem cell transplantation Qual Life Res 2005, ... patient self- report and include: physical and mental functioning, HRQL, psychosocial adjustment, and spiritual well-being Aspects of the symptom experience evaluated in this study include physical and ... questions with a sample of size 80 and an alpha level of 0.05 [12] In that study, no difference in fit between a model that assumed compound symmetry and one that assumed an unstructured covariance...

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Báo cáo y học: "Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cell" ppsx

Ngày tải lên : 10/08/2014, 10:20

... Yarmush ML: Mesenchymal stem cells: mechanisms of immunomodulation and homing Cell Transplant 2010, 19:667-679 Lee PH, Kim JW, Bang OY, Ahn YH, Joo IS, Huh K: Autologous mesenchymal stem cell therapy ... human mesenchymal stem cells generate osteosarcoma-like lesions in the lung Stem Cells 2007, 25:1586-1594 doi:10.1186/1423-0127-18-54 Cite this article as: Chang et al.: Mesenchymal stem cell ... Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation in Experimental Lupus Nephritis Cell Transplant 2011, 20:245-257 32 Parekkadan B, Milwid JM: Mesenchymal stem cells as therapeutics...

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báo cáo khoa học: "The research on the immuno-modulatory defect of Mesenchymal Stem Cell from Chronic Myeloid Leukemia patients" pdf

Ngày tải lên : 10/08/2014, 10:21

... in the endothelial cells of patients with CML, suggesting that CML might originate from hemangioblastic progenitor cells that can give rise to both blood cells and endothelial cells Although Interferon-a, ... by Annexin-V and PI double staining and analyzed by flow cytometry Apoptosis of T cells was analyzed in T cells alone (Ts), normalMSC cocultured with activated T cells (MSC + Ts), and CML patient-derived ... abnormalities, that is, the T lymphocytes in CML might existed in a unusually activated state leading to self injury Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem...

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