modelling neurodegenerative diseases using pluripotent stem cells

Atlas of Human Pluripotent Stem Cells doc

... induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, and especially those related to stem cell-based ... cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early ... ﬂuorescent in situ hybridization (FISH) methods as they apply to the ﬁeld of pluripotent stem cells Induced pluripotent stem cells attract great interest for their potential in understanding the basics...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Danh mục: Báo cáo khoa học

... pluripotent and differentiated cells Nat Biotechnol 2010, 28:1079-1088 54 Sha K, Boyer LA: The chromatin signature of pluripotent cells In StemBook Edited by Girard L Massachusetts: Harvard Stem ... of pluripotent stem cells from fibroblast cultures Nat Protoc 2007, 2:3081-3089 84 Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S: Induction of pluripotent stem cells ... Teitell MA, Pelegrini M, Plath K, Lowry WE: Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini...

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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps

Danh mục: Báo cáo khoa học

... Pluripotent stem cells in neurodegenerative and neurodevelopmental diseases Hum Mol Genet 2010, 19:R71-R76 32 Zhu H, Lensch MW, Cahan P, Daley GQ: Investigating monogenic and complex diseases with pluripotent ... complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte Nat ... Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova...

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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

Danh mục: Báo cáo khoa học

... induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human-induced pluripotent stem cells Stem Cells ... chromosome 12qF1 in mouse induced pluripotent stem cells Nature 2010, 465:175-181 Newman AM, Cooper JB: Lab-specific gene expression signatures in pluripotent stem cells Cell Stem Cell 2010, 7:258-262 ... induced pluripotent stem cells for regenerative medicine Nat Cell Biol 2011, 13:497-505 Zhu H, Lensch MW, Cahan P, Daley GQ: Investigating monogenic and complex diseases with pluripotent stem cells...

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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot

Danh mục: Báo cáo khoa học

... Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent stem ... live cells EMBO J 2007, 26:4252-4262 Shi Y, Do JT, Desponts C, Hahm HS, Schöler HR, Ding S: A com bined chemical and genetic approach for the generation of induced pluripotent stem cells Cell Stem ... mouse induced pluripotent stem cells without viral vectors Science 2008, 322:949-953 Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K: Induced pluripotent stem cells generated without viral...

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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells

Danh mục: Y - Dược

... are pluripotent 42 This technique is common in germ cells because they possess pluripotential characteristics In another instance, pluripotent cells can also be derived from germline stem cells ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs),...

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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages

Danh mục: Cao đẳng - Đại học

... induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers of pluripotency These cells were ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent ... Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent...

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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS 2

Danh mục: Cao đẳng - Đại học

... to detect pluripotent- like stem cells From all the previous experiments and observation, CDy9 can now be safely classified as a compound that selectively stains mouse pluripotent stem cells only ... feeder cells were negative for CDy9 Next immunocytochemistry was carried out for the colonies using pluripotentstem cells specific antibody, anti-oct4 (ab19857) and it was also noticed that these cells ... determine the pluripotent and differentiated states of the cells (refer to Table 2) 21 primers were chosen that define the pluripotent states of the cells These genes are upregulated in ES cells and...

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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS 3

Danh mục: Cao đẳng - Đại học

... induced pluripotent stem (iPS) cells 2008 Cell 134(5): 877–886 9) N Maherali, K Hochedlinger Guidelines and Techniques for the Generation of Induced Pluripotent Stem Cells 2008 Cell Stem Cell ... staining and interaction with the stem cells and a better understanding of what is resulting in pluripotent stem cells to bind specifically to CDy9 as compared to the other cells This may eventually ... induced pluripotent stem cells 2011 J Clin Invest 121(3):1217–1221 33) S W Yun, N Y Kang, and Y T Chang Diversity Oriented Fluorescence Library Approach for Stem Cell Probe Development 2011 Stem Cells...

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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS 1

Danh mục: Cao đẳng - Đại học

... probe for mouse pluripotent stem cells to enable live imaging of the cells with very minimal or no alteration to the cell’s function or capabilities Introduction to stem cells Stem cells are a unique ... function (1 - 3) Typically, stem cells can be broadly classified into main groups, the embryonic stem cells and the somatic or adult stem cells The latter, adult stem cells are found in various ... adult stem cells are hematopoietic stem cells which are formed in the bone marrow These cells play a role by differentiating and replacing the blood cells in the body Other types of adult stem cells...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc

Danh mục: Sức khỏe giới tính

... sources of pluripotent stem cells These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... Kawamata Chapter 21 Modelling of Neurological Diseases Using Induced Pluripotent Stem Cells 413 Oz Pomp, Chen Sok Lam, Hui Theng Gan, Srinivas Ramasamy and Sohail Ahmed Part Pluripotent Alternatives ... pluripotent cells to osteoblasts Part 7: Pluripotent Alternatives Induced Pluripotent Stem Cells (iPSCs) Chapters 18-21 discuss induced pluripotent stem cells (iPSCs) starting with a great review...

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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf

Danh mục: Sức khỏe giới tính

... sources of pluripotent stem cells These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... Kawamata Chapter 21 Modelling of Neurological Diseases Using Induced Pluripotent Stem Cells 413 Oz Pomp, Chen Sok Lam, Hui Theng Gan, Srinivas Ramasamy and Sohail Ahmed Part Pluripotent Alternatives ... pluripotent cells to osteoblasts Part 7: Pluripotent Alternatives Induced Pluripotent Stem Cells (iPSCs) Chapters 18-21 discuss induced pluripotent stem cells (iPSCs) starting with a great review...

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báo cáo khoa học: " Infection, vascularization, remodelling - are stem cells the answers for bone diseases of the jaws?" pot

Danh mục: Báo cáo khoa học

... bone formation of embryonic stem cells and cord blood stem cells in vivo Tissue Eng Part A 2010, 16:2475-2483 Luo JZ, Xiong F, Al-Homsi AS, Roy T, Luo LG: Human BM stem cells initiate angiogenesis ... embryonic stem cells Head Face Med 2008, 4:10 doi:10.1186/1746-160X-7-5 Cite this article as: Handschel and Meyer: Infection, vascularization, remodelling - are stem cells the answers for bone diseases ... Zhang Q, Li Y, Chen C, Chen W, Wang S, Le AD, Shi S: Cell-based immunotherapy with mesenchymal stem cells cures bisphosphonate-related osteonecrosis of the jaw-like disease in mice J Bone Miner...

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Báo cáo y học: " Mesenchymal stem cells in autoimmune diseases: hype or hope" ppt

Danh mục: Báo cáo khoa học

... mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis Arthritis Res Ther 2010, 12:R31 Nauta AJ, Fibbe WE: Immunomodulatory properties of mesenchymal stromal cells Blood ... HH, Betancur M, Wolfberg A, Klingemann H: Umbilical cord mesenchymal stem cells increase expansion of cord blood natural killer cells Biol Blood Marrow Transplant 2008, 14:1031-1038 Shlomchik WD, ... Mönckeberg G, Figueroa FE: Autologous mesenchymal stem cell treatment increased T regulatory cells with no effect on disease activity in two systemic lupus erythematosus patients Lupus 2010, 19:317-322...

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Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates

Danh mục: Cao đẳng - Đại học

... osteoblasts, Targeted cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal ... Cell-material constructs as bone grafts 66 2.4.1 Mesenchymal stem cells (MSCs)-material constructs 67 2.4.2 Non-mesenchymal stem cells (Non-MSCs)-material constructs 69 2.5 Regulating ... important to expedite expansion and differentiation of stem cells into tissue-specific lineages without changing the plasticity nature of the stem cells Various biomaterial fabrication techniques aim...

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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders

Danh mục: Tổng hợp

... Stem cells 1.1.1 Human pluripotent stem cells 1.1.2 In vitro differentiation potential of human pluripotent and neural stem cells 1.2 Candidate diseases ... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... neural stem cells By translating knowledge from developmental neurobiology, protocols to generate distinct neural cell types from pluripotent cells have been established Pluripotent stem cells...

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Tiểu luận glycome chẩn đoán của con người induced cells pluripotent stem sử dụng lectin microarray

Danh mục: Y khoa - Dược

... normal- trung bình (25) Tái lập trình yếu tố chép gây Dẫn đến toàn cầu đảo chiều xuống cho Nhà nước Pluripotent Cellular Glycome Cấp Well-Sử dụng lectin microarray phát triển, phân tích 135 mẫu tế...

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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Danh mục: Y học thưởng thức

... Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymal stem cells are capable of ... transplantation of stem cells, it is very important to observe the survival and curative effects of transplanted stem cells Traditional immunohistochemical method can easily identify the transplanted cells ... expressed in injured tissues play important roles (2) Stem cells circulate among tissues, and stem cells migrate to the injured tissues once injury occurs Stem cell homing is a complicated process in...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Danh mục: Y học thưởng thức

... efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells ... and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems GmbH, Altlussheim, Germany) In the mFISH technique, each chromosome (1–22 and X and Y) is painted a different color, using combinatorial...

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