miniaturization of cell based assays luminescence and fluorescence
mechanical properties of polymers based on nanostructure and morphology, 2005, p.738
... Donald G LeGrand and John T Bendler Handbook of Polyethylene: Structures, Properties, and Applications, Andrew J Peacock Polymer and Composite Rheology: Second Edition, Revised and Expanded, Rakesh ... composites, and biomedical materials by means of electron and atomic force microscopy His special field of interest is the study of toughness enhancement and of the mechanisms of deformation and fracture ... Prediction of Polymer Properties: Third Edition, Revised and Expanded, Jozef Bicerano Fundamentals of Polymer Engineering, Anil Kumar and Rakesh K Gupta Handbook of Polypropylene and Polymer,...
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Báo cáo nghiên cứu khoa học " ACHIEVEMENTS AND LESSON LEARN FROM IMPLEMENTATION OF COMMUNITY-BASED FOREST DEVELOPMENT AND MANAGEMENT " ppt
... experience 3.3.3 Establishment nurseries of village -based One of the constraints in development of both community forest lands and household allocated lands is the lack of quality forest seedlings Some ... confidence to contribute and invest labor and other inputs to the protection and development of community forest lands for overall local benefits Allocation of communication forest lands to communities ... commune and district staff 3.2 % of Community forest land allocated 100 100 100 100 Establishment of community forest management (CFM) plan After receiving the official rights of using the CF lands,...
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Báo cáo sinh học: "The nature of cell-cycle checkpoints: facts and fallacies" docx
... are specific for the different phases of the cell cycle and determine the targets of the kinases Exit from each phase of the cell cycle occurs on degradation of the bound cyclin The CDK-cyclin complex ... Khodjakov and Rieder: Journal of Biology 2009, 8:88 88.5 use because fluorescence- activated cell sorting (FACS) cannot distinguish 4N G2 cells from 4N mitotic cells (or even 4N G1 cells that ... flies Cell 2008, 133:1032-1042 Clute P, Pines J: Temporal and spatial control of cyclin B1 destruction in metaphase Nat Cell Biol 1999, 1:82-87 Gascoigne KE, Taylor SS: Cancer cells display profound...
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Báo cáo y học: "Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury" potx
... contributions AP and BN conceived of the study, participated in its design and coordination, and drafted the manuscript VS and AH carried out the adhesion assays JR and HAH participated in the design of the ... calculated as: *(No of rolling cells) × 100)/(No of rolling cells + No of firmly adherent cells) Mean rolling velocities were determined from more than 25 individual velocity profiles for each experimental ... maldistribution of leukocytes away from local sources of inflammation and towards areas with compromised flow and/ or endothelial damage Abbreviations ANOVA: analysis of variance; ANCOVA: analysis of covariance;...
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Báo cáo y học: "Analysis of cell-based RNAi screens" pdf
... Experimental steps in a cell- based HTS assay Experimental steps in a cell- based HTS assay A cell- based HTS assay consists of a set of experimental steps, shown in the left part of the figure, which ... multi-channel cell- based screens' of the cellHTS package As an example of the analysis of a high-content screening dataset, the vignette 'Feeding the output of a flow cytometry assay into cellHTS' of the ... currently no standard experimental protocols for high-throughput RNAi experiments and, because of rapid developments in RNAi reagents and cell- based assays, we not expect a limited set of standard protocols...
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Construction of physics based brain atlas and its application
... Flowchart of the proposed model 1.3 Anatomy of the Human Head and Brain Head is considered one of the most critical parts of the body A general knowledge of the anatomy and physiology of the head ... structure and properties of soft tissue and second section covers the basics of 41 continuum mechanics which cover issues relevant to the numerical modeling of deformable soft tissue 2.2 Soft Tissue: ... Structure and Properties In this section, we make a brief overview of anatomy and biophysics of brain and other soft tissues with emphasis on their passive mechanical properties 2.2.1 Anatomy of soft...
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Performance analysis of filtering based chaotic synchronization and development of chaotic digital communication schemes
... basins of attractions (basin of attractor of NCA and stable fixed point) As a result of the converging nature of Kalman gain in the basin of attraction of the stable fixed point and the presence of ... 59 4.4 Error dynamics of IM for NPF and EKF based schemes 60 4.5 NMSE of state xR (IM) for NPF and EKF based schemes 61 4.6 TNMSE of IM for NPF and EKF based schemes ... dynamics of Lorenz system for UKF and PF based schemes 46 3.11 NMSE of state x (Lorenz) for the PF, UKF and EKF based schemes 47 3.12 TNMSE of Lorenz system for the PF, UKF and EKF based schemes...
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Development of internet based remote maintenance and telemonitoring system
... Remoting is a combination of technologies enabling the intercommunication of computers of all platforms and languages and software applications of all languages One major advantage of NET Remoting framework ... List of Tables Table 2.1 Benefits of remote maintenance for supplier and customer (from [10]) 12 Table 6.1 Differences between Control Command and Monitoring Data 96 Table 6.2 Configurations of ... research in the area of Internet -based remote maintenance Internet and distributed object technologies are utilized in the design, development, and implementation of the Internet -based remote maintenance...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... encoding regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell- based, in vitro and in silico experiments ... just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based and in silico analysis) will facilitate systematic understanding of the underlying ... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software...
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Báo cáo sinh học: "High ERCC1 expression predicts cisplatin-based chemotherapy resistance and poor outcome in unresectable squamous cell carcinoma of head and neck in a betel-chewing area" pdf
... expression of ERCC1 As can be seen in Table a summary of result of the ERCC1 immunostaining of the cancer cells and its correlation with the clinicopathologic variables, the high and low ERCC1 ... the date of start of chemotherapy to the date of death or most recent follow-up Progression free The median age of the patients was 53 years (range 3672 years), and fifty-five (96.5%) out of 57 ... surgery and standard radiotherapy alone [12] In Taiwan, for public healthy insurance, cisplatin is the backbone of the chemotherapy regimen as a component of IC and CCRT in the treatment of locally...
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báo cáo hóa học:" High ERCC1 expression predicts cisplatin-based chemotherapy resistance and poor outcome in unresectable squamous cell carcinoma of head and neck in a betel-chewing area" pdf
... expression of ERCC1 As can be seen in Table a summary of result of the ERCC1 immunostaining of the cancer cells and its correlation with the clinicopathologic variables, the high and low ERCC1 ... the date of start of chemotherapy to the date of death or most recent follow-up Progression free The median age of the patients was 53 years (range 3672 years), and fifty-five (96.5%) out of 57 ... surgery and standard radiotherapy alone [12] In Taiwan, for public healthy insurance, cisplatin is the backbone of the chemotherapy regimen as a component of IC and CCRT in the treatment of locally...
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Báo cáo y học: "Apoptotic cell-mediated suppression of streptococcal cell wall-induced arthritis is associated with alteration of macrophage function and local regulatory T-cell increase: a potential cell-based therapy" ppsx
... accumulation of mononuclear cells with release of proinflammatory cytokines and erosive destruction of subchondral and periarticular bone and cartilage [18-20] Systemic administrations of IL-4, TGFβ ... Institute of Dental and Craniofacial Research, National Institutes of Health All animal studies were performed according to National Institutes of Health guidelines for use and care of live animals and ... test the efficiency of apoptotic cell infusion to modulate the arthritic response The feasibility of cellular therapy based on the immunomodulatory properties of apoptotic cells has already been...
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Báo cáo y học: "ptimized design and data analysis of tag-based cytosine methylation assays" pps
... MSCC [15] and HELP-seq [10] assays, not only by means of technical modifications such as the use of EcoP15I but also because of the concurrent use of MspI for normalization The effect of these ... cytometry with SSEA4, CD24 and Oct4 markers To extract DNA, the cells were suspended in 10 ml of a solution of 10 mM Tris-HCl (pH 8.0), 0.1 M EDTA and ml of 10% SDS to which 10 μl of RNase A (20 mg/ml) ... success of these projects, while the careful ongoing assessment of potential sources of bias will also be essential for improving assay performance Materials and methods Cell preparation and DNA...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays
... bovine serum and 10 μg/mL insulin in a humidified atmosphere of 5% carbon dioxide in air at 37°C 53 2.3 ERα and ERβ cell- based bioassays 2.3.1 Establishment of ERα and ERβ stable cells (from Yap ... and 10 nM of icariin, icaritin and desmethylicaritin; 5, 30 and 60 nM of icariside I and 71 icariside II, respectively and internal standard in pooled rat sera, were placed at the beginning and ... Optimization of ERα and ERβ cell- based bioassay parameters ERα and ERβ cells were developed by Yap et al., 2005 and optimization of bioassay parameters was performed by seeding cells at various...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 2
... steroid 89 3.1.3 Validation of ERα and ERβ cell- based bioassays The inter- and intra-assay variations of both ERα and ERβ bioassays, were determined by using 0.1 nM and 0.5 nM of estradiol, respectively ... 175 200 [E2], pM 92 3.1.4 Isoform selectivity of DPN, PPT and genistein To assess the ER-isoform selectivity of ERα and ERβ bioassays, compounds such as, DPN, PPT and genistein, which are non-steroidal ... estrogenic activity using ultra-sensitive, cell- based ER-driven reporter gene assays 3.1.1 Responses of ERα- and β-driven reporter gene assays to estradiol and its metabolites To examine their response...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 4
... ERα and ERβ proteins respectively were selected for the development of ERα and ERβ cell- based bioassays The production of stable cell lines eliminates the necessity of constantly producing DNA and ... determined to be 8.1% and 16% respectively Detection limits for estradiol in ERα and ERβ bioassays were 8.4 and 13.1 pM, respectively This set of assays is simple and rapid to perform and offers highthroughput, ... understand biological effects of estrogenic compounds, alone and in combination 4.1.1 Ultra-sensitive bioassays expressing ERα and ERβ can be used to detect ERisoform selective activity and study of...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 5
... analytical methods coupled with cell- based bioassays that can measure global estrogenicity of sera of animals were employed to understand the levels and bioactivity of conjugated and unconjugated active ... levels of estrone and estradiol by tandem mass spectrometry, for ERα and ERβ bioactivity and MCF-7 breast cancer cell proliferative effects The concentrations of icaritin and desmethylicaritin and ... bone, endometrium and breast, the next step is to develop and validate a panel of such cell- based bioassays Even within the same cell line, differences exist in the ability of different compounds...
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Study of pharmacokinetics of prenylflavonoids and dynamics of estrogen action in sera following ingestion of epimedium using validated, ultra sensitive cell based bioassays 6
... Brouwer, A and van der Burg, B 2005 Development of androgen- and estrogen-responsive bioassays, members of a panel of human cell line -based highly selective steroid-responsive bioassays Toxicol ... C.X and Chen, Y.M 2009 Effect of long-term intervention of soy isoflavones on bone mineral density in women: a meta-analysis of randomized controlled trials Bone 44(5):948–953 Liu, J., Ye, H and ... 27008–27016 Mak, P., Cruz, F.D and Chen, S 1999 A yeast screen system for aromatase inhibitors and ligands for androgen receptor: yeast cells transformed with aromatase and androgen receptor Environ...
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Control of Cell Proliferation and Growth byMyc Proteins
... deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting that the balance of expression of members of the ... in cell proliferation: for example, deletion of n-myc in neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of ... generally required for cell proliferation and cell growth or whether its essential role in proliferation is restricted to specific cell types Control of Cell Proliferation and Growth by Myc Proteins...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25