microstructure and properties of in situ toughened silicon carbide
Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) Such concerted reactions result in formation of ... consisting of covalent crosslinks of proteins beneath the plasma membrane of terminally differentiated keratinocytes [23,24] Furthermore, TGase in hair follicles is involved in crosslinking structural ... Detection of in situ TGase activities in the mouse skin section Hematoxylin and eosin staining is shown at the left FITC– pepE51 (1 lM) was reacted with frozen mouse skin section in the presence of...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... constant of trypsinolysis was determined by plotting ln(At ⁄ Ao) (where Ao and At denote the intensity of protein bands of intact unhydrolized protein at the beginning and at the fixed time of incubation) ... HSP22 and its mutants, we plotted ln(At ⁄ Ao) (where Ao and At are the intensities of the band of intact protein at the beginning of trypsinolysis and at the fixed time of trypsinolysis) against ... Reduction of the disulfide bonds of insulin results in dissociation of its peptide chains and aggregation of chain B Addition of the wild-type HSP22 retarded the onset of aggregation and decreased...
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Tài liệu Nature and Properties of Micro-organisms doc
... penicillins, cephalosporins and vancomycin lipoteichoic acid, septic shock Beta-lactamases hydrolyse penicillins and cephalosporins secreted by Gram positive bacteria Within periplasm of Gram ... invasiveness of pathogenic bacteria by reducing opsonisation and protecting from phagocytosis E.g Streptococcus pneumoniae in RTI vaccination: pneumococcal; meningococcal A and C GLYCOCALYX ... bacteria, shorter and finer than flagella Two classes: ordinary pili ‘colonisation antigens’ Protein, attachment to host cells Can be involved in host cell invasion e.g Neisseria meningitidis ...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... GTAAAACGACGGCCAGT Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... sipV Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... the indicated temperature by the addition of 35S[methionine] (50 mCiáL)1) and chased by the addition of nonradioactive methionine and cysteine (2.5 gáL)1) Samples were collected at intervals and...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... determine the unique functions of these avin proteins? In this study, we present the crystal structure of DvMF avoredoxin and discuss the key residues for ligand binding and metal ion binding, ... avin-binding domain of ferredoxin : NADP+ reductase [10] The lysine residue is not conserved; instead, an arginine residue interacts with the FAD molecule of the enzyme In the case of FMN-binding ... reductase and avin reductase activities FeR and Fre activity were examined using avoredoxin, DrgA [13] and FMN-binding protein [14] DrgA from Synechocystis sp PCC6803 and FMN-binding protein from...
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Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inflammatory mediators ppt
... target (Fig 1) Indeed, IL-1ra reduces brain injury in animal models of cerebral ischemia and, in a recent randomized clinical trial, intravenous administration of recombinant human IL-1ra in patients ... K & Gearing A (2001) Immunohistochemistry of matrix metalloproteinases in reperfusion injury in rat brain: activation of MMP-9 linked to stromelysin-1 and microglia in cell cultures Brain Res ... PJ, Hopkins SJ & Rowland M (2008) Pharmacokinetic modelling of interleukin-1 receptor antagonist in plasma and cerebrospinal fluid of patients following subarachnoid haemorrhage Br J Clin Pharmacol...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... determination of molecular mass of tetrathionate hydrolase in SDS/PAGE Proteins were stained by GelCode Blue Stain Reagent (Pierce, Rockford, IL, USA) and the SilverXpress Silver Staining Kit (Invitrogen) ... by GelCode Blue Stain Reagent and SilverXpress Silver Staining Kit in SDS gel electrophoresis (Fig 1) Isoelectric focusing in the 2D gel detected a single spot and the pI of the tetrathionate ... of the high NaCl concentration (1 M) Most of the remaining proteins in the extract did not bind to the cation exchange resin in the presence of NaCl and tetrathionate hydrolase was then eluted...
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Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx
... (taurocyamine kinase, TK), phospholombricine (lombricine kinase, LK), phosphoopheline (opheline kinase, OK), phosphohypotaurocyamine (hypotaurocyamine kinase, HTK) and phosphoarginine (arginine kinase, ... (30% of that of taurocyamine) and glycocyamine (7% of that of taurocyamine) Recombinant Arenicola MiTK was incapable of phosphorylating creatine even though this TK has a higher degree of sequence ... alignment of Fig (arrow b) is strictly conserved in each of phosphagen kinases, namely Arg in CK, Ile in GK, Tyr in AK and Lys in LK While this residue is not directly involved in substrate binding in...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... mL of a 0–0.15 M linear gradient of NaCl in buffer C, at a flow rate of mLÆmin)1, collecting mL fractions The F 4¢-OMT-containing fractions were pooled, desalted through dialysis and the obtained ... plot of inhibition kinetic of AdoHcy The obtained experimental data at increasing inhibitor Ó FEBS 2003 3428 P Curir et al (Eur J Biochem 270) Table Kinetic parameters of S-adenosyl-L-methionine:flavonoid ... of inhibition kinetic of S-adenosyl-Lhomocysteine (AdoHcy) Lineweaver–Burk plot of 1/v vs 1/[s] (where s ¼ kaempferol) in the presence of different fixed concentrations of S-adenosyl-L-methionine...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... of low-Mr GTP-binding proteins, in that in addition to the DXXG sequence present in the cloning primer, they encoded the GXXXXGK(S/T), NKXD and EXSA amino-acid residues also involved in binding ... Blue-stained gel showing 10 lg of platelet protein and 0.5 lg of GST and GST±Rab proteins; (B) an [a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 ... 0.5 lg of GST and 0.5 lg of GST±Rab proteins, except for GST±Rab5A (0.1 lg); (C) an immunoblot of a gel containing 20 lg of platelet protein and 0.1 lg of GST and GST±Rab proteins, using a :...
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Photonic Properties of Er-Doped Crystalline Silicon
... apply Vinh et al.: Photonic Properties of Er-Doped Crystalline Silicon greatly lower the binding energy due to the interactions between O and Si, and also O and Er, atoms, thus enabling the incorporation ... screening; therefore the transitions are only slightly allowed and recombination times remain longVin the millisecond range In an extreme case, for Er3þ in an insulating host Proceedings of the ... Restrictions apply Vinh et al.: Photonic Properties of Er-Doped Crystalline Silicon (Bx[ is the position of the line in the spectrum) This value is in good agreement with the separation of lines L2 , L2...
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Mechanics and Properties of Composed Materials and Structures doc
... effects of masonry, reinforced concrete and soil, such as yielding in compression, cracking in tension, opening and closing of cracks, tensile and shear stress of cracking material, etc The macro and ... yielding and crushing in compression, the soil cracking in tension, anisotropic soil properties and other nonlinear soil effects according to Sects 3.1 and 3.2 3.5 Modelling of the Strain Rate ... horizontal and vertical direction, tensile and shear stiffness of cracked masonry, • Concrete yielding in compression, opening of cracks in concrete in tension, mechanism of opening and closing of cracks...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... describe the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are compared ... (Novagen) in the absence of IPTG HMS-F was routinely cultured in the presence of 10 lgÆmL)1 tetracycline to maintain carriage of the F episome E coli strains were cultured in L-broth and on L-agar ... electroporated into the E coli strain HMS-F [29] The resulting ampicillin- and tetracycline-resistant transformant was cultured with and without IPTG, and expression of a (His)6-tagged protein of the...
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preparation and properties of magnetic iron oxide nanotubes
... in sample S2 (c and d) and ␥-Fe2 O3 nanotubes in sample S3 (e and f) B Lv et al / Particuology (2008) 334–339 and outer and inner diameters of 70–120 nm and 45–80 nm, respectively Fig 3(c) and ... bonds between adsorbed phosphate ions and iron ions would form spin pinning and block spin flip conversion, directly resulting in the increase of coercivity of the samples Second, the nanotube structure ... López-Urías, and Mu˜ oz-Sandoval (2005) n simulated the micromagnetic property of iron nanorings, and they found large coercive fields for din /dout > 0.5 (din and dout are the inner and outer diameters of...
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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
... Albert Einstein College of Medicine, New York The E coli strains TOP10 and BL21Star (DE3) (Invitrogen) were used for cloning and expression studies, respectively E coli strains were cultured in Luria–Bertani ... activity of TreS was routinely measured by determining the formation of reducing sugar when enzyme was incubated with trehalose Assays were carried out in a final volume of 100 lL, containing 40 ... Q-TOF MS, and identified in the M smegmatis genome (shown in bold type and underlined) These peptides allowed the gene for TreS to be identified in the genome and its cloning and expression in...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... S-transferases contain two sites for substrate binding; a G-site for the binding of glutathione and a H-site for the binding of hydrophobic substrate S-hexylglutathione has previously been shown to bind to ... W G Willmore and K B Storey 49 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem ... occur in many stress-tolerant organisms For example, the maximal activities of GST increased during anoxia exposure in brain of the leopard frog Rana pipiens [17] but decreased during freezing in...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... emulsions of both medium and long chain triacylglycerols (Fig 3) Areas of olive oil and tricaprylin hydrolysis are clearly seen and correspond with the single protein band stained in native-PAGE gel In ... containing lipase were divided into 35 mL aliquots and stored at )80 °C prior to chromatography Preliminary experiments to investigate binding properties of the lipase, using ion exchange resins, ... We chose to examine the effect of Ca2+ loss on stability by incubating LipA in the presence and absence of Ca2+ and examining effects over time Concentrated enzyme preparations in 30 mM Tris/...
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Báo cáo khoa học: "Smart Paradigms and the Predictability and Complexity of Inflectional Morphology" docx
... Nouns in French have two forms (singular and plural) and an intrinsic gender (masculine or feminine), which we also considered to be a part of the in ection table Most of the unpredictability comes ... qmul.ac.uk/˜mpurver/software.html one third of the nouns of the lexicon were not included in the experiment because one of the form was missing The vast majority of the remaining 15,000 nouns are ... paradigms and the guess heuristics are implemented manually and with a high certainty; in unsupervised learning of morphology the paradigms are induced from the input forms with much lower certainty Of...
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Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248±254 14 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the ... proteinase Bovine chymotrypsin and trypsin are stabilized by calcium [23,24] The addition of 2.5 lgámL)1 of BSA per lgámL)1 of proteinase was suf®cient to completely maintain the proteinase ... proteinase, whose production by F culmorum was induced/enhanced in the presence of grain protein, has been puri®ed from growth medium and characterized The properties and the amino-acid sequence of...
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Sythesis and properties of spinel NiAl2O4, CoAl2O4, ZnAl2O4
... ô sở: 2+ a = 5,790 + 0,95R + 2,79 R 3+ Tồn dạng cấu trúc: spinel thông thường (nornal spinel) spinel ngược (inverse spinel) Spinel thường Ion B3+ chiếm lỗ trống bát diện, chiếm phân nửa ... Phương pháp tiền polymer hóa Pechini thích hợp cho việc tổng hợp spinel NiAl2O4, CoAl2O4 , ZnAl2O4 cho chất lượng độ kết tinh cao Phổ XRD cho thấy chất thu cấu trúc tinh khiết Ảnh chụp SEM cho ... A2+ chiếm 1/8 lỗ trống tứ diện Nếu ion có kích thước gần lượng mạng tinh thể lớn Ví dụ: loạt spinel nhôm MgAl2O4… Spinel nghịch Ion A2+ chiếm lỗ trống bát diện Ion A2+ chiếm 1/8 lỗ trống...
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