microinjection of baboon sperm
Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... recognizes both isoforms each of the isoforms of interest to confirm cellular localization In contrast to results observed with the homologous mouse SMO isoforms, each of the three human isoforms examined ... each enzyme was capable of oxidizing spermine as opposed to N1-acetylspermine With both enzymes, the kcat value of spermine is approximately 27-fold that of N1-acetylspermine Spd Spm * * * v ... where the possibility of detoxification of the ROS prior to damage is reduced Additionally, the oxidation of spermine to spermidine diminishes nuclear spermine pools Because spermine has essential...
- 12
- 395
- 0
Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf
... effects on sperm function, including a diversity of enzymes, hormones, growth factors and transport proteins [6] However, the precise role of most of the seminal plasma proteins in sperm physiology ... sperm physiology remains obscure Spermadhesins are a family of 12–16 kDa proteins found in seminal plasma and ⁄ or attached to the spermatozoal surface of a variety of mammalian species (e.g boar, ... the presence of mm CaCl2 (Table 1), emphasizing the specificity of the effect of Zn2+ Thermal destabilization of PSP-I ⁄ PSP-II was also noticed at acidic pH (Fig 2B) in the absence of Zn2+ cations...
- 8
- 387
- 0
Identification of baboon microRNAs expressed in liver and lymphocytes pptx
... (pre-miRNA) sequences with draft baboon genome sequence data to identify putative baboon miRNAs After identifying the baboon miRNAs in-silico, we determined expression profiles of baboon liver and lymphocytes ... profiling for baboon liver and lymphocyte RNA miRNA Expression Figure The number of mismatches compared to the number of predicted and expressed baboon miRNAs The x-axis indicates the number of ... status of the draft baboon genome sequence and that use of a microarray from a closely related species is important to discovering miRNA genes of an unannotated genome Our discovery of baboon...
- 8
- 177
- 0
báo cáo khoa học: "Angiofibroma of the spermatic cord: a case report and a review of the literature" potx
... Angiofibroma of the spermatic cord: a case report and a review of the literature Journal of Medical Case Reports 2011 5:423 Submit your next manuscript to BioMed Central and take full advantage of: ... angiofibroma: a benign neoplasm distinct from angiomyofibroblastoma and spindle cell lipoma Am J Surg Pathol 1997, 21:636-644 Laskin WB, Fetsch JF, Mostofi FK: Angiomyofibroblastomalike tumor of ... of the scrotal and inguinal area, is rich in fibroblasts, and of vascular origin A safe initial diagnosis is difficult because of its location, nature, and correlation with other structures of...
- 4
- 385
- 0
Báo cáo y học: " Angiomyofibroblastoma of the spermatic cord: a case report" pptx
... Angiomyofibroblastoma of the male inguinal region Arch Pathol Lab Med 2000, 124:1679-1681 Laskin WB, Fetsch JF, Mostofi FK: Angiomyofibroblastomalike tumor of the male genital tract: analysis of ... Scully RE: Angiomyofibroblastoma of the vulva and vagina Mod Pathol 1996, 9:284-291 Laskin WB, Fetsch JF, Tavassoli FA: Angiomyofibroblastoma of the female genital tract: analysis of 17 cases including ... angiomyxoma: reappraisal of its relationship to angiomyofibroblastoma in a series of 16 cases Histopathology 1997, 30:3-10 10 Malik A, Singh KJ, Mehta A: Aggressive angiomyxoma of the spermatic cord:...
- 4
- 449
- 0
GROWTH PERFORMANCE AND SPERM QUALITY OF STRESS NEGATIVE PIÉTRAIN BOARS AND THEIR HYBRIDS WITH DUROC
... ejaculation volume (VOL), spermatozoon motility (MO), sperm concentration (CO) and total number of spermatozoon in ejaculate (NT), rate of abnormal spermatozoon (R) and pH of semen (pH) VOL was determined ... (GLM) procedure of SAS software (SAS 1989) in order to identify significant sources of variation The least-squares means were compared using Tukey HSD tests from each genetic group The sperm quality ... coefficient of determination 219 Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Table Production performance, Least square means (LSMSE) of PiDu...
- 6
- 734
- 0
Tài liệu Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis) pptx
... properties of muscle phosphofructokinase Mol Cell Biochem 57, 147–154 Hand SC & Somero GN (1983) Phosphofructokinase of the hibernator Citellus beecheyi, temperature and pH regulation of activity ... Phosphofructokinase: mechanism of the pH-dependent inactivation and reactivation of the rabbit muscle enzyme J Biol Chem 251, 5630–5636 Bock PE & Frieden C (1976) Phosphofructokinase: role of ligands ... skeletal muscle of the golden-mantled ground squirrel (Spermophilus lateralis) Particular attention is paid to the effects of temperature on enzyme allostery and the influence of temperature in...
- 9
- 579
- 0
Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
... pancreatic ducts is of considerable significance in the pathology of a number of diseases including cystic fibrosis and cancer Equally important is the fate of the cytoplasmic tail of the mucins once ... is of particular interest as we, and others, have detected multiple forms of the cytoplasmic tail of MUC1, MUC1FDTR and its derivatives [9,13] Further, the migration of the CT2-reactive forms of ... modeling of SEA domain of MUC1 The composer and biopolymer modules of the sybyl 6.91 software package (Tripos Inc., St Louis, MO, USA) were used for comparative molecular modelling of the human...
- 11
- 605
- 0
Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... of intact sperm recovered in Study Sperm counts tallied both intact sperm and sperm heads not attached to a tail The number of intact sperm was expressed as a percentage of the total number of ... a Sperm Count Index of 6.0 with a mean sperm count of 67 ± million motile sperm per cauda epididymis As the higher Sperm Count Index indicated, a much larger proportion (7/8) of this group’s sperm ... contains many spermatocytes (sp), round spermatids (rs) and condensing spermatids (cs) Tails (t) of condensing spermatids and newly released testicular sperm are seen in the lumen (Lu) of some tubules...
- 15
- 967
- 0
Báo cáo khoa học: The role of histones in chromatin remodelling during mammalian spermiogenesis pot
... extent than in spermatids Therefore the identification of HIRA partners in spermatids would be of great interest in understanding the molecular basis of histone replacement during spermiogenesis ... up to 55% of the total linker histones Mice bearing invalidated H1t gene display no phenotype [14–16], but the analysis of enriched populations of pachytene spermatocytes and round spermatids ... Chromatin code and spermatogenesis (Eur J Biochem 271) 3461 Fig Sequence analysis of known histone variants expressed during spermatogenesis The sequences of conventional histones and their spermatogenic...
- 11
- 616
- 0
Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf
... abolishes proper nuclear targeting of the mSMOl isoform Results Structural analysis and modeling of vertebrate SMO proteins Comparison of the derived amino acid sequences of vertebrate SMO proteins has ... mammalian-specific function of SMO (e.g nuclear targeting of the mSMOl isoform) Inspection of the mSMOl modelled structure (Fig 2) indicates that both regions are located on the tip of the FAD-binding ... sequence comparison of members of the spermine oxidase (SMO) and polyamine oxidase (PAO) family (A) Amino acid sequence alignment of the SMO and PAO proteins (B) Alignment of the deduced amino...
- 8
- 413
- 0
Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... pCI-neo /baboon CYP17 cDNA The activity of baboon CYP17 was determined in HEK-293 cells transfected with pCI-neo /baboon CYP17 cDNA Expression of the recombinant enzyme permitted the investigation of ... chemicals were of reagent grade purchased from scientific supply houses Determination of the cytochromes P450 and b5 contents of baboon adrenal microsomes Microsomes were prepared from baboon adrenal ... CYP17 cDNA RT-PCR amplification of baboon mRNA, using baboon specific primers complementary to the nucleotide sequence encoding the amino and carboxy terminal of baboon 5612 A C Swart et al (Eur...
- 9
- 420
- 0
Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc
... What is the origin of the high level of GSH in spermatogeinc cells? If GSH is important for the protection of spermatozoa from oxidative damage, why is the GR content so low in spermatogenic cells? ... block of GSH, is required for spermatozoa, the presence of GSH presents somewhat of an obstacle due to its reducing power As the biosynthetic rate of protamine production is very high during the spermiogenic ... oxidation of sulfhydryls is related to the acquisition of motility [4,5] During epididymial migration, sulfhydryls in the nucleus and the tail of spermatozoa are oxidized to disulfides Levels of antioxidative...
- 9
- 494
- 0
Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
... Determination of the DCA-binding sites of histone H2B Because the carboxamide group of Gln9 of histone H2B is selectively transamidated with the e-amino group of Lys5 or Lys12 of histone H4 in ... chromatin of starfish sperm, Gln9 of histone H2B and Lys5 or Lys12 of histone H4 must be in close proximity This may have important implications for the chromatin organization in the sperm of starfish ... and H4, are substrates of an endogenous nuclear transglutaminase in starfish sperm nuclei In this study, we also showed that linker histone of starfish sperm was a substrate of the nuclear transglutaminase...
- 10
- 503
- 0
Báo cáo khoa học: "Effect of ethylene glycol monoethyl ether on the spermatogenesis in pubertal and adult rats" pdf
... the control Effects of EGEE on the spermatogenesis of different age of male rats 49 Fig Epididymis weight of pubertal (a) and adult (b) rats administered with various doses of EGEE for weeks Weights ... of cells actively synthesizing Effects of EGEE on the spermatogenesis of different age of male rats DNA The haploid region can be split into two peaks based on the differential stainability of ... round/elongated spermatids The chromatin of the elongated spermatids is highly condensed and binds less to fluorescent dye when compared to that of the round spermatids The elongated spermatids appear...
- 5
- 296
- 1
Báo cáo khoa học: " Testicular morphology and cauda epididymal sperm reserves of male rats exposed to Nigerian Qua Iboe Brent crude oil" ppsx
... sa puorg tnemtaert hcae rof detaluclac erew sthgiew ralucitset dna sevreser mreps lamydidipe aduac eht rof rorre dradnats dna naeM sisylana lacitsitatS ypocsorcim thgil rof nisoe dna nilyxotameh ... erew snoitces kciht mµ ehT gninoitces rof xaw niffarap ni deddebme dna ,enelyx ni deraelc ,lonahte fo slevel dedarg ni detardyhed erew yeht ,retaL h 84 rof diulf s’niuoB ni noisremmi yb dexif ... tup gnieb erofeb ecnalab reltteM a gnisu dehgiew erew yehT demmirt erew seussit suoenartxe dna detcessid erew spuorg tset dna lortnoc eht ni star fo sedimydidipe eht ,htaed gniwolloF stnemerusaem...
- 5
- 173
- 0
Báo cáo khoa học: "A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen" pptx
... μg/mL of heparin, 10 mg/mL of bovine serum albumin (BSA) to evaluate sperm quality and to use as 100 μL-droplets of spermatozoa for IVF Evaluation of sperm For evaluation of progressive motile sperm, ... may be due to differences among the sperm separation techniques in the removal of spermatozoa damaged in the plasma-membrane of the sperm head (CFDA/PI) and the sperm tail (HOST) These results indicate ... 10 μL of diluted semen was placed on a clean microscope slide, and covered with a coverslip The percentage of progressive motile spermatozoa was determined by observing a minimum of 300 sperm, ...
- 7
- 496
- 2
báo cáo khoa học: "Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa" ppt
... may often lead to the loss of functional competence of the spermatozoa The major advantage of AFM in pathological studies of spermatozoa is that it allows the evaluation of position and form of ... spectrum of the above profile showing the scaling of roughness d Histogram plot of the height of the head region e 3D image of the head region of the spermatozoa mechanical response of the cell ... normal human spermatozoa AFM of AFM of normal human spermatozoa a 2D image (8.00 × 8.00 µm scan) of normal sperm head b Height profile of the head region of spermatozoa showing clear difference...
- 6
- 363
- 0
Báo cáo khoa học: "Morphology and head morphometric characters of sperm in Thai native crossbred stallions" potx
... software (Oval Metrix Version 4.18) Recognition of sperm and the rejection of other cells were performed at an accuracy consistent with the hardware and software specifications The analysis software ... equine sperm microscopy was 60× 200 morphologically normal sperm heads were acquired in each test, and consequently a total of 800 sperm were analyzed for each animal The software reported five sperm ... significantly different (P > 0.05) in respect of percentage of sperm that exhibited normal morphology However, the percentages of each type of morphologically abnormal sperm were significantly different...
- 9
- 283
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008