mice show increased levels and activities of matrix metalloproteinases and reduction of sirt1 levels and activity as well as enos levels in lungs by cs

Báo cáo y học: "Suppression of LPS-induced matrixmetalloproteinase responses in macrophages exposed to phenytoin and its metabolite, 5-(p-hydroxyphenyl-), 5-phenylhydantoin" pptx

Ngày tải lên : 11/08/2014, 03:20

... MMP-9 and TIMP-1 (Fig 1D and Fig 2) Levels of Figure The effect of phenytoin, HPPH and LPS on levels of (A) matrix metalloproteinase-1, (B) matrix metalloproteinase-2, (C) matrix metalloproteinase-3, ... This finding is in agreement with in- vitro and in- vivo studies which report a relative reduction in MMP-1 and MMP-8/TIMP-1 in gingival fibroblasts and in serum and GCF concentration in CSA-associated ... elevated levels of PHT in LPS-stimulated macrophages were associated with decreases in both MMP and TIMP levels Therefore the decrease in TIMP-1 levels was counteracted by decreases in MMP levels As...

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Báo cáo y học: " High sensitivity C-reactive protein is associated with lower tibial cartilage volume but not lower patella cartilage volume in healthy women at mid-life" pdf

Ngày tải lên : 09/08/2014, 10:23

... any of the following: a clinical diagnosis of knee OA as defined by American College of Rheumatology criteria, knee pain lasting for > 24 h in the last years, a previous knee injury requiring ... identified by number, not by name, and the study was conducted in accordance with the Declaration of Helsinki principles Measurement of hsCRP Measurement of hsCRP was performed using a particle ... imaging (MRI) has good tissue contrast and anatomical resolution [7], thus allowing a non-invasive examination of the joint structure in pre-disease or early stage OA MRI can visualize joint...

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Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot

Ngày tải lên : 17/03/2014, 10:20

... decrease in ATP and the increase in glucose 6-phosphate in yeast grown in glucose could be explained by an enhanced activity of hexokinase produced by both the release of its inhibition by trehalose ... charge, ATP and inosine were determined as described in Materials and methods H2O2 evokes a decrease and an increase in the intracellular concentration of ATP and inosine, respectively Searching for ... the main if not unique source of inosine is the intracellular pool of adenine nucleotides, (b) the decrease in ATP and the increase in inosine, promoted by H2O2, cannot be explained solely by a...

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Báo cáo y học: "Aspergillus fumigatus allergen expression is coordinately regulated in response to hydrogen peroxide and cyclic AMp" doc

Ngày tải lên : 13/08/2014, 13:22

... 1:200) was induced after 24 h but decreased after 48 Page of 11 h of incubation Since Asp f is a ribotoxin and Asp f is a peroxiredoxin, the up-regulation of genes coding for these proteins was expected ... expected Genes involved in protein synthesis and folding, such as Asp f 8, 11 and 23 were also induced during the first 24 h as expected In contrast, lower MOI (> 1:2000) caused repression of several ... fumigatus is well studied as an invasive pathogen of humans [17-19] but is also a major source of fungal allergens involved in allergy and exacerbations of asthma such as Severe Asthma with Fungal...

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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx

Ngày tải lên : 07/03/2014, 15:20

... two independent effects (Table 1) Using control as the baseline, perfusion with 0.25 or 0.5 mM palmitate increased a-1 AMPK activity by at least 3-fold and a-2 AMPK activity was increased by at ... epinephrine Epinephrine increased the rate of cardiac lipolysis measured as glycerol accumulation in the perfusate from 0.13 ± 0.03 to Fig Eﬀects of palmitate, insulin and epinephrine on a-2 AMPK activity ... that an increase in serum NEFA after 24 h of starvation was accompanied by increased AMPK activity and P-T172 abundance in vivo This increase in AMPK activity is likely to be a contributing factor...

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Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot

Ngày tải lên : 23/03/2014, 06:20

... activation of these proteases, the activation of caspase being transient and the activation of caspase being stronger and sustained over time In addition, the length of activation of caspase is similar ... we examined the time course of activation of caspases by cellular stress, we found that activation of caspase is greater and more sustained than that of caspase (Fig 2E) Moreover, caspase activation ... signiﬁcantly induced Other protease activities, including caspase 8, calpain or cathepsin B, were not affected Activation of the effector caspase and caspase suggests that hDlg could be proteolysed by...

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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf

Ngày tải lên : 23/03/2014, 10:21

... minimizing cell injury and maintaining tissue integrity in response to damaging levels of an environmental agent As such, the integrity of this system plays a role in modifying progression of diseases ... in media containing Geneticin to select for cell containing vector DNA (as in Fig 5A,G) After three weeks, the number of cells was determined by ﬁxing cells and staining with dye Individual colonies ... cells were split and plated in media containing Geneticin to select for cell containing vector DNA After three weeks, the number of cells was determined by ﬁxing cells and staining with dye: vector...

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Báo cáo sinh học: "Metabolic reconfiguration is a regulated response to oxidative stress" pdf

Ngày tải lên : 06/08/2014, 18:21

... and tandem mass spectrometry) to show that inactivation of TPI results in increased concentrations of pentose phosphate pathway metabolites Importantly, they found that the more that TPI was inhibited, ... and 6PGDH enzyme activities have been shown to be maintained in yeast cells during oxidant exposure [13,14] Direct evidence that flux through the pentose pathway is increased during oxidative stress ... provided by measurements of the NADPH/NADP+ ratio, which showed that reducing TPI activity shifts the redox ratio towards a more reducing state; this state is important for maintaining antioxidant activity...

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báo cáo khoa học: " The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism" potx

Ngày tải lên : 11/08/2014, 11:21

... http://www.biomedcentral.com/1471-2229/11/108 h, peaking by h and then falling to basal levels within before increasing between 12-24 h (Figure 1A-d) To determine if AtTSPO accumulation was a transcriptional response ... Gabaculine and Norflurazon Gabaculine acts as a tetrapyrrole biosynthesis inhibitor by blocking the glutamate-1-semi aldehyde aminotransferase activity [30,31] The herbicide Page of 17 Norflurazon inhibits ... localization of TSPO in both chloroplasts and mitochondria is consistent with its role in porphyrin trafficking Plant TSPO has been proposed to participate in the interaction between plastid and mitochondrial...

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báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt

Ngày tải lên : 11/08/2014, 11:21

... 15-day-old pVvMYB60:GUS seedling (E) Magnification of leaf primordia in (D), showing staining of differentiating stomata (F) Detail of a pVvMYB60:GUS mature leaf, showing staining of fully differentiated ... cells in Arabidopsis, and is down-regulated by ABA (A) 15-dayold pVvMYB30:GUS seedling (B) Magnification of leaf primordia in (A), showing staining at the base of trichomes (C) Detail of an emerging ... expression in pre- and post-fertilization flowers from most pVvMYB60:GUS lines In two transgenic lines, a weak staining was occasionally detected in stamens, at the interface of filaments and anthers...

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Báo cáo y học: " The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent" potx

Ngày tải lên : 12/08/2014, 14:20

... cytoplasmic staining pattern, resembling that observed in the staurosporine control, which increased in a time-dependent manner (Figure 4C-D) At puffs GPS, cytoplasmic staining appeared as early as ... rest of the samples, as the number of puffs increased Active caspase-3 was absent from the sample exposed to puffs Equal loading was verified by probing the samples analysed with an anti--tubulin ... pertaining to the cytoplasmic release of cytochrome C and the subsequent activation of caspase-3, collectively pointed towards the activation of the caspase-3 dependent apoptotic pathway in a...

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Báo cáo y học: " Research Upregulation of CD23 (FcεRII) Expression in Human Airway Smooth Muscle Cells (huASMC) in Response to IL-4, GM-CSF, and IL-4/GM-CSF" pot

Ngày tải lên : 13/08/2014, 13:22

... exists in the phenotypic expression of the ASMC in culture [25] In addition to the combination of IL-4/GM-CSF inducing increased expression of CD23, both IL-4 and GM-CSF alone independently increased ... IL-13, and IL-5, as well as other enzymes and chemokines are active in the asthmatic patient GM-CSF has been shown to be involved in asthma pathogenesis and in vivo can induce TH2 differentiation independent ... supporting the role of γc chain in CD23 expression by IL-4 The signs of signal transduction in response to IL-4, and the increase in protein content of the cell in response to IL-4 and GM-CSF combination...

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Báo cáo y học: " The histone deacetylase Rpd3p is required for transient changes in genomic expression in response to stress" ppt

Ngày tải lên : 14/08/2014, 21:20

... these loci increased in wild-type cells, but not the mutants In contrast, both the promoter and open reading frame of iESR gene XKS1 showed increased histone acetylation and nucleosome loss in wild-type ... [5], and protein kinase C (Scott Topper and APG, unpublished) pathways in response to osmotic shock, DNA damage, or reducing agents, respectively, and the protein kinase A and target of rapamycin ... genes within the ESR Indeed, here we show that Rpd3p is required for proper initiation of the ESR, including normal regulation of both induced and repressed genes, in yeast responding to multiple...

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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1

Ngày tải lên : 24/08/2014, 12:43

... within a column A Bradford Assay was used to measure the protein concentration of the post binding wash, and there was no detectable protein in the solution (data not shown) indicating 100% binding ... will make up the column bed and act as the stationary phase and the solution containing the antibody of interest will act as the mobile phase In most instances as well as in this study, the antigen ... cytoplasm and the nucleus as opposed to NF-κB which was almost exclusively localized in the cytoplasm In the presence of IL-1, the concentration of SIMPL in the nucleus was increased, and in the...

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Báo cáo y học: "TPO, but not soluble-IL-6 receptor, levels increase after anagrelide treatment of thrombocythemia in chronic myeloproliferative disorders"

Ngày tải lên : 03/11/2012, 10:52

... with at least 50 % of the baseline value, and treatment failure (F) as a reduction of the platelet count with < 50 % of the baseline value Here, and for the purpose of cytokine levels and platelet ... Vainchenker W, Wendling F Prominent role of TGF-beta in thrombopoietin-induced myelofibrosis in mice Blood 2002;100:3495-503 32 Erusalimsky JD, Franklin R and Hong Y Is the platelet lowering activity ... dose was 0.5 mg b.i.d If there was no response the dose was increased by 0.5 mg per day per week The mean maintenance dose was 2.3 mg/day Complete response (CR) was defined as fulfillment of the...

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Báo cáo khoa học: "Kerbs von Lungren 6 antigen is a marker of alveolar inflammation but not of infection in patients with acute respiratory distress syndrome" docx

Ngày tải lên : 13/08/2014, 10:20

... KL-6 was measured by ELISA (Eisai Corporation, Tokyo, Japan) according to the manufacturer's instructions in BALF and in plasma The intra-assay coefficient of variation was 5.1% and the inter-assay ... in epithelial lining fluid [8,9] It has been suggested that an increase in circulating KL-6 levels in interstitial pneumonitis is therefore due to an increase in KL-6 production by regenerating ... aetiology of lung injury The presence of active alveolar infection was defined by the presence of >104 colony-forming units of pathogenic bacteria in BALF (irrespective of mode of injury) A significant...

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Báo cáo y học: "Genetic polymorphism of p53, but not GSTP1, is association with susceptibility to esophageal cancer risk – A Meta-Analysis"

Ngày tải lên : 25/10/2012, 11:40

... polymorphisms and asthma at the 0.05 level of significance, assuming a genotypic risk of 2.0 and 1.5 The Egger’s test and inverted funnel plots were utilized to provide diagnosis of publication bias (Linear ... Origin Ethnicity Lin DX&[28] 1998 China Asian Morita S[29] 1998 Japan Asian van Lieshout EM[30] 1999 The Netherlands Caucasian Tan W&[31] 2000 China Asian Lee JM[22] 2000 China(Taiwan) Asian Casson ... with increased esophageal SCC risk [40], it is worth mentioning that there are main forms of esophageal cancer histologically, squamous cell carcinoma (SCC) and adenocarcinoma, and each has distinct...

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In order to become competent in a foreign language, it is important for language learners not only to acquire new vocabularies and a new set of phonological and syntactic rules but also to learn what Wilson (1986)

Ngày tải lên : 17/04/2013, 16:09

... Phước, Tây Ninh thành phố Hồ Chí Minh; ĐBSCL gồm tỉnh Long An, Tiền Giang, An -18- Giang, Kiên Giang, Cà Mau, Sóc Trăng, Bạc Liêu, Đồng Tháp, Bến Tre, Hậu Giang, Vónh Long, Trà Vinh thành phố ... lớn, nước ròng… Nam Bộ vùng đất đa dạng sinh học Khí hậu - thuỷ văn tạo điều kiện cho động thực vật sinh sôi nảy nở, thích hợp cho việc phát triển nguồn sinh vật cạn nước, thuận lợi cho canh tác ... lớn nước Ven biển có rừng ngập mặn rộng lớn Từ Cà Mau đến Kiên Giang có rừng nguyên sinh U Minh Thượng, U Minh Hạ Rừng có nhiều loài động thực vật quý Hình ảnh quen thuộc sân chim, kèo ong, sếu...

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Tài liệu Báo cáo khoa học: Activated Rac1, but not the tumorigenic variant Rac1b, is ubiquitinated on Lys 147 through a JNK-regulated process docx

Ngày tải lên : 18/02/2014, 16:20

... function and resulting in increased invasive properties of the pathogens [5,6] Finally, dysregulations of Rho GTPases have been observed in a variety of human tumors [7]; overexpression of Rho GTPases, ... accumulated in cells expressing UbR48 (Fig 1B), indicating that, in the absence of Lys48, the chain cannot be extended Consistently, inhibition of proteasome using MG132 resulted in an increase of polyubiquitinated ... arginine codons AGA and AGG respectively Rac1L61G189 was obtained by mutation of the cysteine codon TGC into the glycine codon GGC In all cases, the absence of additional mutations was veriﬁed by...

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Cytoskeleton reorganization mediates alpha beta integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells pdf

Ngày tải lên : 05/03/2014, 17:20

... gene by insulin and luteinizing hormone in cultured porcine granulosa-luteal cells: possible convergence of protein kinase a, phosphatidylinositol 3-kinase, and mitogen-activated protein kinase ... an increase in cell death, a decrease in progesterone secretion and an increase in estradiol secretion Previous investigations using inhibitors of actin microfilament polymerization, such as ... important functional changes such as a decrease in proliferation rate and an increase in pyknotic rate of GC, as well as a decrease in progesterone and an increase in estradiol secretion (p < 0.01 for...

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