methylation in embryonic stem cells in vitro pdf
báo cáo hóa học:" Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro" pdf
... Medicine 2009, 7:6 http://www.translational-medicine.com/content/7/1/6 CD34+ Figure stem cells transdifferentiated in muscle cells in vitro CD34+ stem cells transdifferentiated in muscle cells in ... amplified in co-cultures, indicating the absence or very low expression of dystrophin in co-cultures (data not shown) Transdifferentiation of CD34+ stem cells into muscle cells During the expansion ... when murine bone marrow stem cells fused to murine embryonic stem cells [29] However, the real meaning of fusion versus transdifferentiation is still controversial [30-33] Adult stem cells transplantation...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... Foundation, and Parkinson's Action Network, as well as the Cancer Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent ... funding of research involving the more than 60 existing stem cell lines, but will not sanction or encourage destruction of additional human embryos Indeed, the embryos from which the existing ... Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004, 114:1364-1370 Donovan PJ, Gearhart J: The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation-related ... detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the...
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Báo cáo hóa học: " Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro" pdf
... doxorubicin was contained in the culture to maintain K562/AO2 cells drug resistance in its daily culture The inhibition of cell growth was measured by MTT (Microculture Tetrazolium) assay Initially, ... attracted a great deal of attention in fabricating continuous ultrafine fibers or fibrous structures for various polymers, with typical examples including engineering plastics, biopolymers and polymer ... leukemia cells and lead to the much higher intracellular drug concentration in the target cells, resulting in the remarkable enhancement of the intracellular fluorescence of drug-resistant leukemia cells...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s ... hindering the access for the long form protein, thus having an advantage to be evolutionarily selected and maintained Since the truncated protein itself does not contain the c-terminal cysteines, ... predicted open reading frame Thin lines indicate exon-exon junctions A The thick line points to the junction of exon and skipping exon in the truncated form but is included in the long form of...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types Reprod Biomed Online ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... units up to 0.4 mm in diameter was established Feeder-independent murine embryonic stem cells (ESCs) were kindly provided by K Pfeffer (Institute for Microbiology, Heinrich Heine University of...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
... sources including autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic ... BMP-2 Shown are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without ... The increase in reaction products during PCR was monitored by measuring the increase in fluorescence intensity caused by the binding of SYBR green to doublestranded DNA that accumulated during...
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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain, ... ZNF8 genes with expression in seasonal H1N1 infected cells being lower than pdmH1N1 infected cells was maintained Over-representation analysis using InnateDB To determine the biological relevance ... seasonal influenza viruses in its induction of cytokine responses in human macrophages and epithelial cells [2-4] This suggests that the cytokine dysregulation seen in H5N1 infection is not an intrinsic...
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Báo cáo y học: "Thymic plasmacytoid dendritic cells are susceptible to productive HIV-1 infection and efficiently transfer R5 HIV-1 to thymocytes in vitro" pdf
... After rinsing in PBS, the sections were exposed to biotinylated-mAb (Vectastain, Vector Laboratories Inc., Burlingame, CA) for 30 min, rinsed with PBS and then incubated with Steptavidin-HRP ... virus proteins within the DC during a spreading infection (Figure 3B) In comparison to thymic DC cultures, we did not detect a statistically significant difference in EGFP+ cells in blood pDC ... for 30 Immunostaining was revealed using 3,3’-Diaminobenzidine substrate solution according to the manufacturer’s guidelines (Dako) Sections were counterstained with haematoxylin and blued with...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... by genotyping The ES cell lines were maintained in the undifferentiated state by culturing on gelatinized plates in KO-DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with leukemia inhibitory ... chromatin components In this regard, compensatory chromatin modifications have been previously described, including an increase in H3K27me3 levels in Suv39h1/h2 deficient ES cells [25] Minor and ... was seen in Dicer KO cells (17 compared with in matched wild-type cells) Increased minor and major satellite transcripts were also seen in Eed deficient ES cells but not in other mutant lines that...
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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc
... system in generating genome-wide homozygous mutations to facilitate recessive genetic screens in vitro by identifying mCat-1 as a critical gene in ES cells that is required for retroviral infection ... high infection efficiency must be achieved so that every single infectable cell in the culture has at least one infection event In practice, the need to infect every cell in a culture of 109 cells ... on ES cells after a single round of infection with 0.1 ml viral supernatant are illustrated by staining puromycin-resistant colonies in 24-well plates Figure (see following page) Super-infection...
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Dissecting transcriptional network in mouse embryonic stem cells
... undifferentiated cells and differentiated cells of multiple lineages EC cells could be maintained indefinitely with mitotically inactivated embryonic fibroblast in vitro, and is able to give rise to cells ... state of ES cells for in vitro feeder free culture, in vivo ICM cells are able to develop into ES cells in the absence of LIF signaling, indicating that alternative pathways might be involved Recent ... These cells were initially maintained in culture as self-renewal and pluripotent cell lines in either EC cell-conditioned medium (Martin, 1981), or in a co-culture system in which cells were grown...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... effective in vitro system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells ... importance of SHH in early mammalian development, there is significant value in studying its function in human embryonic stem cells (hESC), which are in vitro counterparts of the inner cell mass...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... joint consensus motif in the Oct4 and Sox2 ChIP-PET datasets 6.5 Binding of Oct4 and Sox2 overexpressed (OE) proteins on biotin-labelled DNA probes containing Oct4 and Sox2 binding sites using ... protein kinase (MAPK) and extracellular signal regulated kinase (ERK) protein kinase; SMAD: similar to mothers against decapentaplegic homologue; SHP2: SH2-domain-containing protein tyrosine phosphate-2; ... repression in somatic cells and loss of Gcnf-induced restriction of Oct4 in the germ line The same phenotype is observed in Gcnf-deficient mice containing a targeted deletion of the DNA-binding domain...
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Regulation of gene expression by esrrb in embryonic stem cells
... cells Multipotent stem cells can give rise to cells of certain specialized lineages Many adult stem cells are multipotent, including hematopoietic stem cells, mesenchymal stem cells, and other ... embryonic tissues to extra-uterine sites resulted in teratocarcinoma formation (Solter et al., 1970; Stevens, 1970) This finding indicates the embryonic origin of teratocarcinoma stem cells In ... nuclear factor homeobox containing protein family, inner cell mass inhibitor of differentiation insulin-like growth factor receptor JmjC domain-containing proteins leukaemia inhibitory factor liver...
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The role of kruppel like factors in embryonic stem cells
... transcripts zinc finger protein 42 zinc finger protein 143 zinc finger protein of the cerebellum XI Introduction CHAPTER I INTRODUCTION Introduction CHAPTER I INTRODUCTION Stem cells can be identified ... CHAPTER I INTRODUCTION 1.1 Pluripotent stem cells 1.1.1 Mouse embryonic carcinoma cells 1.1.2 Mouse embryonic stem cells 1.1.3 Mouse embryonic germ cells and pluripotent spermatogonial stem cells ... to activation of a MAP kinase signaling cascade, including MAP kinase, MAP kinase kinase (MKK), and MAP kinase kinase kinase (MKKK) 16 Introduction Much evidence has indicated that the Ras-Mek-Erk...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... staining (b) H1 von Kossa staining (c) hFOB H & E staining (d) hFOB von Kossa staining Figure At 4-week time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... applications 1.2.2.2 Embryonic stem cells: Another type of stem cells by conventional categorization is embryonic stem cells based on its origin from the fertilized egg In 1981, embryonic stem cells (ESC) ... somatic cells They repeatedly introduce certain proteins channeled into the cells via poly-arginine anchors and sufficiently induced pluripotency[45] This new technique brings in new hope in stem ... These included substituted purines, pyrimidines, indoles, quinazolines, pyrazines, pyrrolopyrimidines, pyrazolopyrimidines, phthalazines, pyridazines and quinoxalines[55] High-throughput screens...
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