methods for structural and chemical characterization of nanomaterials
Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers Previous structural analysis of the ... jellyroll domain formed by two b-sheets and a distal domain formed by insertions between the strands of the jellyroll domain (Fig 3) Antiparallel b-sheets made up of strands CHEF and BIDG pack against ... purification, and chemical analysis of adenovirus Virology 20, 199–207 Pettersson U, Philipson L & Hoglund S (1968) Structural proteins of adenoviruses II Purification and characterization of the adenovirus...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... assignments of conserved glycine amide protons, at position six of the 12-residue long calcium-binding loops of EF-hand proteins, have chemical shifts 10 p.p.m for filled loops and chemical shifts of ... to dimerize and both EF-hands of CR I–II bind calcium We conclude that the significant structural and biochemical differences between CR I –II and Calb I –II are related to a region of poor sequence ... data for ‘position 6’ glycine residues also support calcium-binding to both EF-hands of CR I–II (Fig 6) but only to EF-hand I of Calb I– II These glycine chemical shifts report the formation of...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
... the residues of S100 A4 critical for S100 A4 dimerization [23] The amino-acid sequence of calhepatin was aligned with those of the human form of each S100 protein, except for MRP126 and ictacalcin, ... sequencing, and by a dotted line arrow for those inferred on the basis of their amino-acid analysis Each peptide is labelled with a letter (E for peptides derived by Glu-C digestion and K for peptides ... equation, where v is the number of moles of Ca2+ bound per mol of monomer, x is the free Ca2+ concentration, n is the number of binding sites per monomer, and Ka1 and Ka2 are macroscopic binding...
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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
... appear as a loose network of thin bers with occasional formation of bundles (Fig 5A) Bundle formation differs between actin isoforms and is a function of polymer concentration and ionic strength [21] ... composed mainly of a helices (Figs and 2) The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) anking a ... concluded that EF-hand of the Iba proteins is not capable of functional Ca2+ binding Function of EF-hand In both Iba1 crystal structures, the loops of EF-hand adopt essentially the same conformation (Fig...
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Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx
... HPLC, Denise LeBlanc for loan of a size exclusion HPLC column, Steve Locke for trypsin, and Neil Ross for time on his fluorimeter and patient instruction in software use for the instrument We ... intensity of a higher-molecular-mass band The apparent molecular mass of this band was 38 kDa, which is substantially higher than the molecular mass of the monomer band, indicating the presence of a ... monomer bands of 18 kDa and dimer bands of 33 kDa To examine whether Ca2+ binding was required for dimerization, smelt AFP was incubated with BS3 in buffer containing 10 mM EDTA instead of CaCl2...
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Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf
... room at 10 V cm–1 for 2–5 h RESULTS AND DISCUSSION 3.1 Sequencing and structural characteristics of the pine GS1 genomic clone A λgt10 subgenomic library of Scots pine was screened for GS clones ... pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5’region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation codon was isolated ... tissues of developing seedlings and the level of the transcript was affected by developmental and light conditions [9] Here, we present the DNA sequence of a partial genomic clone containing exons of...
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Báo cáo y học: "Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments" pot
... crucial role of shorter NT-domain in the biological function of apoE and provide the basis for the explanation of diverged functionality of truncated apoE isoforms Materials and methods Plasmids ... protein isoforms in terms of structural stability was in the order of apoE2 > apoE3 > apoE4 Previous structural studies indicated that Cys112 of apoE3 is partially buried between helices and 3, ... level of p = 0.95, a best fitted average anhydrous friction ratio (fr), a resolution value N of 200, and sedimentation coefficients between and 20 S For the data fitting of apoE-(72-166) in PBS and...
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Development of microextraction based techniques for quantification and behaviour characterization of nanoparticles in aquatic environments
... My first and foremost gratitude goes to my supervisor, Professor Hian Kee Lee, for his continuous and unconditional support of my Ph.D study and research, invaluable suggestions, and for his patience ... (circle) and zeta potential (triangle) (a), and release of Cu2+ (diamond) (b), for 100 µg L-1 CuO NPs with 0.2% w v-1 of TX-114 and incubation at 40 °C for 45 Error bars show standard deviations of ... water chemistry and temperature on the behavior and fate of ENPs in aqueous media A review of the application and environmental implications of NPs, and characterization, separation, and quantification...
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INFLUENCE OF STRUCTURAL AND CHEMICAL ASYMMETRY OF NANOSTRUCTURES ON THE KINETICS OF WETTING
... nanostructures and energy losses in the form of skin drag and form drag It was found that skin drag tends to be stronger along the length of the nanofins while the converse is ix true for form drag Therefore, ... not for the following institutions and people Singapore-MIT Alliance, both the offices and the program, for the provision of a bond-free scholarship and excellent administrative execution Prof ... angle (θc) for silicon nanopillars 143 Table 5-2: Geometrical parameters of nanofins used in this study where h refers to the height of the nanofins, and definitions of p, q, m and n can be...
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Structural and epitope characterization of major allergens from dust mite, BLO t 21 and DER f 7
... STRUCTURAL AND EPITOPE CHARACTERIZATION OF MAJOR ALLERGENS FROM DUST MITE, BLO T 21 AND DER F TAN KANG WEI (B Sc., UKM) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... characterization and ligand binding studies of Der f will be presented as well These results may pave a way for understanding the allergenic properties of these proteins, and aid in the development of hypoallergens ... characterized for its 3D structure and IgE epitope (Gajhede, Osmark et al 1996; Mirza, Henriksen et al 2000; Spangfort, Mirza et al 2003) The 3D structure of Bet v consists of β-strands and α-helices forming...
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Structural and functional characterization of TRX16, a thioredoxinlike protein and altering substrate specificity of a serine protease inhibitor
... structure, as structure and function go hand in hand Decades of effort using X-ray crystallography and NMR have produced thousands of protein and complex with binding partner structures and these structures ... can express the profound respect and gratitude I have for my supervisors Prof K Swaminathan and Prof J Sivaraman I thank them for their perpetual guidance, unceasing cooperation and constant encouragement, ... integration of structural biology, chemistry, molecular biology and genetics, has much to offer for the modern day chemical and pharmaceutical industries Protein engineering is undergoing the most profound...
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Structural and binding characterization of the antiviral host proteins, VIPERIN and VAPC
... beta-strand, for random coil and -1 for α-helix (Figure 1.10) strand, 14 Figure 1.9 Chemical shift analysis of the peptide backbone NMR signals Each panel represents a chemical shift analysis of the ... Alignment of the amino acid sequences of hVAP-C with hVAP-B and hVAP-C 64 Figure 3.1.7 Model for the mechanism of formation of HCV replication complex on lipid raft 68-69 Figure 3.3.1 Purification profiles ... torsion angles Ф and χ1, 3JNHHα and 3JHαHβ are used thus providing important information on conformations of peptide backbone and amino acid side chains Deviation of 3JNHHα values from random coil...
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Structural and functional characterization of haditoxin, a novel neurotoxin isolated from the venom of ophiohagus hannah (king cobra
... (Selected as “Paper of the Week” and for the cover page illustration for the March 12, 2010 issue of the JBC) (4) Roy A, Rajagopalan N, and Kini RM Identification and characterization of a α-helical ... continent except for Antarctica and few islands such as Ireland, Iceland, and New Zealand (Conant and Collins, 1991) Fifteen families are currently recognized, comprising 456 genera and over 2,900 ... scope for nicotinic acetylcholine receptor ligands 49 vii 1.13 Aim and scope of the thesis 50 CHAPTER TWO: ISOLATION AND PURIFICATION OF HADITOXIN 52 2.1 INTRODUCTION 52 2.2 MATERIALS AND METHODS...
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Structural and functional characterization of signaling protein complexes
... N Number of binding sites Nav Voltage gated sodium channel isoform Nav1.4IQ IQ motif of voltage gated sodium channel isoform 1.4 Nav1.6IQ IQ motif of voltage gated sodium channel isoform 1.6 ... modelling Complex formation, crystallization and data collection Cloning of CaM, ΔNav1.6 Expression of ΔNav1.6 and association with CaM Expression and purification of CaM Pull down assays and gel filtration ... model for memory formation, and the early phase of LTP indicates the start of memory formation 14 1.4.3 ERK/MAPK pathway in late LTP Maintaining LTP in activated neurons is essential for the formation...
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Design, structural and functional characterization of human beta defensin analogs
... Expression and purification of Def-A and rHBD-3 in LB and M9 media 39 44 2.2.1 Media (LB and M9) used for the expressions 44 2.2.2 Expression of peptides in M9 media 44 2.2.3 Purification of peptides ... intensities and connectivities and the solution structure of SDS bound Def-A (A) Relative intensities and connectivities of NOEs and (B) Superposition of 10 lowest energy structures for SDS bound ... 2.1 Reagents and volumes used for the PCR 40 Table 2.2 Temperature cycles and time intervals used for each cycle 40 Table 2.3 Volumes of different DNA substrates and enzymes used for double digestion...
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Structural and epitope characterization of dust mites allergens, der f 13 and blo t 5
... isolation 12 1.3 Structural and epitope characterization of allergens 1.3.1 Structural biology of allergens Structural studies of allergenic proteins are essential in identification of structural attributes ... Mechanism of allergy 1.2 Dust mite, an important source of allergens 1.3 Structural and epitope characterization of allergens 13 1.3.1 Structural biology of allergens 13 1.3.2 IgE epitope mapping of ... activation of two main tyrosine kinases, Lyn and Syk ITAMs of β and γ chains will be phosphorylated by Lyn and followed by recruitment and activation of Syk to the ITAMs of γ chains (Abbas and Lichtman,...
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Different physical and chemical pretreatments of wheat straw for enhanced biobutanol production in simultaneous saccharification and fermentation
... Experiment and 2, 1.6 g of wheat straw was mixed in 50 ml of sterilized distilled water in 100 ml shaker flask and autoclaved at 135°C for h During Experiment and 4, 1.6 g of wheat straw in 50 ml of ... sample tube format and CytoSoft Software with expandable software modules Samples were diluted 20-fold using deionized water The diluted samples were repeatedly vortexed at high speed for and thereafter, ... Chemist Soceity and Institute of Food Technology She is a current Ph.D candidate Yaser Dahman holds a Ph.D in Chemical and Biochemical Engineering from the University of Western Ontario and an MBA...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... strongly on the local environment of the amino acid and is a sensitive probe of conformation in the vicinity of tryptophan residues 5888 The binding of ANS and other hydrophobic probes to a protein ... studies of wild-type and mutant Hsp25 The thermostability of wild-type and mutant Hsp25 was investigated by monitoring the increase in light scattering at 360 nm as a result of the formation of large ... rate of °CÆmin)1 Wild-type Hsp25 and Q194A remained in solution up to temperatures of 100 °C By contrast, the E190A, E199A and E204A mutants precipitated out of solution within °C of the onset of...
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Aircraft measurements over Europe of an air pollution plume from Southeast Asia – aerosol and chemical characterization pot
... adsorption of nitric acid on the wall of the sampling tube, respectively The accuracy of the NO and NOy measurements is and 15%, respectively, for a time resolution of s Detections of CO and O3 were ... Much of our current understanding of the impact of intercontinental air pollution transport on the chemical composition of the atmosphere is based on the results of model studies (e.g Wild and ... positive band of CO (Gerbig et al., 1996) and UV absorption (Thermo Electron Corporation, Model 49), respectively The accuracy of the CO and O3 measurements is 10 and 5% for a time resolution of s...
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Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... DIPEA/DMF for coupling and piperidine/DMF for Fmoc deprotection Coupling of Fmoc-Ala-Ala to Fmoc-Hypro Wang resin (50 mg) was performed manually and then loaded onto the automated synthesizer for the ... addition of 10 lL 10% aqueous trifluoroacetic acid and immediately injected onto RP-HPLC and fractions collected were collected and analyzed with ESI and MALDI-MS Chemical reduction The native and ... increments in t1 and spectral widths of 8000 Hz and 17 591 Hz in the 1H and 13C dimensions, respectively A total of 256 summed scans were collected with a relaxation delay of 1.3 s All 1H and 1H-13C...
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