measurements of threshold of mitochondrial permeability transition pore opening in intact and permeabilized cells by flash photolysis of caged calcium
Tài liệu Báo cáo khoa học: Mitochondrial chaperone tumour necrosis factor receptor-associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening docx
Ngày tải lên :
16/02/2014, 14:20
... hypoxia-induced cell death in primary cultured cardiomyocytes Cell death was determined by incubating normoxic cells, hypoxic cells, vector-infected hypoxic cells and Ad-TRAP1-infected cells after ... opening by inhibiting the activity of the peptidyl-prolyl cis-trans isomerase of CypD [27,28] The consequences of MPTP opening are cell necrosis and apoptosis and, even if MPTP opening is insufficient ... accompanied by an increase in MPTP opening [32– 34] It is considered that Dw reflects the state of MPTP opening indirectly In the present study, we found that silencing TRAP1 induces Dw loss in cardiomyocytes,...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf
Ngày tải lên :
16/02/2014, 09:20
... specifically inhibits PTP opening by binding to cyclophilin D in the matrix and on the inner surface of the IM [51–54] Growing evidence has implicated MPT in the necrotic and apoptotic death of hepatocytes ... swelling of liver and heart mitochondria by cyclosporine is probably caused by the inhibitor binding to mitochondrial- matrix peptidyl-prolyl cis–trans isomerase and preventing it interacting with ... vector-transfected cells and mutant HSS-expressing cells Similarly, CCCP-induced apoptosis was also inhibited by CsA Effect of HSS on alteration of wm Fig (A) The level of HSS RNA in the three types of cells...
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Báo cáo khoa học: Changes in specific lipids regulate BAX-induced mitochondrial permeability transition pptx
Ngày tải lên :
07/03/2014, 05:20
... to mPTP -opening induced by rBAX To gain insight into the existence of putative mitochondrial lipid microdomains in vivo, we evaluated the inducing effect of rBAX on opening of the mPTP in kidney ... that BAX induces opening of the permeability transition pore (mPTP) [12] The opening of this mega-channel would lead to mitochondrial swelling and rupture of the outer membrane, thus explaining the ... (2001) Opening of the mitochondrial permeability transition pore causes depletion of mitochondrial and cytosolic NAD+ and is a causative event in the death of myocytes in postischemic reperfusion of...
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Báo cáo khoa học: The mitochondrial permeability transition from in vitro artifact to disease target ppt
Ngày tải lên :
30/03/2014, 11:20
... [108] Binding of ubiquinone (Ub0) or decylubiquinone prevents Ca2+-dependent pore opening, irrespective of the inducing agent; and the inhibitory effect of Ub0 and decylubiquinone (but not that of ... Lisa F (1999) Transient and longlasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence Biophys ... subsequently obtained by methods allowing the detection of PTP opening in 2086 intact cells and tissues [71,112,205,206] Occurrence of PTP opening was assessed through the (re)distribution of molecules...
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báo cáo khoa học: "Regulation of cell cycle transition and induction of apoptosis in HL-60 leukemia cells by lipoic acid: role in cancer prevention and therapy" ppt
Ngày tải lên :
10/08/2014, 22:20
... Following 24 and 48 h treatment of HL-60 cells with different concentrations of LA (0, 2.5, and mM), cells were washed with PBS and stained with 1.0 μg/ml DAPI containing 100 mM NaCl, mM MgCl2 and ... evident by the appearance of sub-G1 fraction (Fig 2A); notably, the percentage of apoptotic cells increased from 1.4% in control cells to 59.6% and 72.9% in 24 and 48 h, 2.5 and mM LA-treated cells, ... AIF and cytochrome c (Cyt C) expression in 48 h LA treated cells (D) The actin-adjusted level of bax and bcl-2 and changes in the ratio of bax to bcl-2 in HL-60 cells treated for 48 h with increasing...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
Ngày tải lên :
07/03/2014, 04:20
... estimation of proline and ornithine J Biol Chem 199, 91–95 45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956) Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methyl-guanidine ... considerable and significant activities of AGAT and arginase II in the three types of tumor cells prompted us to measure the level of GAA and ornithine in tumor cells Table shows that the level of ornithine ... low in these cells Thus, we studied the uptake and release of creatine in EAC and S180 cells as a model system Figure shows that, if the cells were incubated for h in the uptake medium containing...
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Effects of compost and phosphate amendments on arsenic mobility in soils and arsenic uptake by the hyper
Ngày tải lên :
16/03/2014, 00:07
... has lowered the drinking water standard from 50 to 10 mg lÀl, effective nationally by 2006 Arsenic-contaminated soil is one of the major sources of arsenic in drinking water (Hingston et al., 2001) ... spiking of As into the soil, in which As was predominantly associated with the labile exchangeable and aluminum oxide fractions ($ 80% of total As) On the other hand, in the CCA, soil As was mainly ... arsenicals by a reductive pathway from inorganic and methylated forms of As (Onken and Adriano, 1997) Akins and Lewis (1976) added DSMA-74As to a soil system and measured a loss of 74As The loss of 74As...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
Ngày tải lên :
30/03/2014, 15:20
... of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in U937 cells ... phospho-STAT3 increased only in U937 cells treated with 10 nM leptin (Fig 3A) In addition, nuclear levels of Ikaros isoforms Ik1, Ik2 and Ik3 were increased by treatment of U937 cells, but not of CHP100 cells, ... leptin (Fig 1A) and progesterone (Fig 1B) increased FAAH mRNA in a dosedependent manner in human U937 cells, in a way parallel to that of enzymic activity and protein content The effect of leptin...
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Báo cáo hóa học: " Research Article Recognition of Nonprototypical Emotions in Reverberated and Noisy Speech by Nonnegative Matrix Factorization" potx
Ngày tải lên :
21/06/2014, 07:20
... IS, N31, and IS+N31 feature sets in the graphs in Figure and leave out N30 After obtaining an optimized value of C for each training condition, we joined the training and development sets and used ... testing condition, while the −5 dB level was excluded from training Thus, the multicondition training, as well as training with BA or ST noise, involves the union of training data corresponding ... Particularly, in the case of ST training and testing on BA, we observed a gain of 2.5% absolute over the baseline (62.77%) by both the N31-1 (65.28%) and N31 (65.27%) EURASIP Journal on Advances in Signal...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
Ngày tải lên :
09/08/2014, 01:24
... concentration in joint fluid was measured by a sandwich binding protein assay using a Hyaluronate-Chugai test kit [33] The molecular weight of hyaluronan was calculated from the intrinsic viscosity of hyaluronan ... expression of hyaluronidase-2 in the synovium of OA and RA was approximately four times that in the control synovium This finding suggests that in OA and RA, the protein expression of hyaluronidase-2 in ... RA), since a high expression level of HAS-3 message in inflammatory cells was observed in another study by two of us (NI and KK) It is supposed that the hyaluronan produced by inflammatory cells...
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Multi-beneficial for mitigation of climate change in Vietnam and Indochina countries by development of biomass energy project
Ngày tải lên :
18/03/2015, 13:20
... MULTI-BENEFICIAL FOR MITIGATION OF CLIMATE CHANGE IN VIETNAM AND INDOCHINA COUNTRIES BY DEVELOPMENT OF BIOMASS ENERGY PROJECT The 1st Joint Coordinating Com m ittee (JCC) M eeting January 7th, 2013 Vietnam ... Chemistry JO IN T C O O R D INA TING C O M M IT T E E (JCC) M E ET IN G AGENDA Time: Venue: 09:0C January 07, 2013 Faculty of Chemistry, VNU-HN _ y Morning Opening Greeting - Prof Luu Van ... Root, le a f and stem fresh w e ig h t of Jatropha plant cultured in vitro in L and 60 L ve sse ls on day 55 Root, le a f and stem dry w e ig h t of Jatropha plant cultured in vitro in L and 60 L...
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Regulation of sla2p by activity in endocytosis and actin organization by the ark1 prk1 family of kinases
Ngày tải lên :
14/09/2015, 08:50
... NPFs in yeast and their domain structures The actin binding domains are highlighted in red (WH2 is Pan1p and Las17p, ADFH in Abp1p and TH2 in Myo3p and Myo5p) and the Arp2/3 binding acidic domain ... capping proteins and Sac6p, an actin bundling protein (Moseley and Goode, 2006) The various steps of the maturation of an actin patch have been delineated by visualizing live cells by tagging ... Abp1p contains an N-terminal ADF/cofilin homology domain that binds to actin, two acidic sequences and a C-terminal SH3 domain The actin binding activity 10 CHAPTER I INTRODUCTION of Abp1p is...
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Báo cáo khoa học: Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation doc
Ngày tải lên :
16/03/2014, 06:20
... small increase in the internal calcium concentration, which in turn activates the uniporter of calcium, increasing even more the calcium in ux and ROS production Both the increase of intramitochondrial ... swelling induced by MccJ25, strongly suggesting that the peptide was able to induce mitochondrial swelling through the opening of the MTP mediated by the uptake of calcium through the uniport of calcium ... calcium On the other hand, KCN and antimycin A, both inhibitors of electron transport, also inhibited the swelling induced by MccJ25 The inhibition of swelling induced by ascorbic acid will be...
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Báo cáo Y học: Permeability transition-independent release of mitochondrial cytochrome c induced by valinomycin ppt
Ngày tải lên :
17/03/2014, 10:20
... swelling in the presence of Ca2+, SF6847, and valinomycin Panels A, B, and C represent effects of Ca2+, SF6847, and valinomycin, respectively, on the mitochondrial structure and function Upper and ... valinomycin on mitochondrial respiration and volume and their sensitivity to PT inhibitors When a mitochondrial PT is induced, acceleration of mitochondrial respiration and swelling of mitochondria ... accompanying swelling, and CsA and BKA were ineffective in preventing accelerated respiration (Fig 1B) In contrast, as shown in Fig 1C, valinomycin accelerated the rate of oxygen consumption, and the accelerated...
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Báo cáo Y học: Changes in ultrastructure and the occurrence of permeability transition in mitochondria during rat liver regeneration ppt
Ngày tải lên :
24/03/2014, 04:21
... permeability [19] Such a mitochondrial swelling was inhibited by the addition to the mitochondrial suspension of CsA (Fig 5A), the specific inhibitor of the permeability transition pore of mitochondria ... change in permeability of the inner mitochondrial membrane due to Ca2+ loading was accompanied by a nonspecific release of mitochondrial proteins in the suspension medium [28] with the appearance, in ... problems for mitochondria and cells [43] The mitochondria in intact cells may undergo permeability transition and swelling in a fully reversible manner without progressing to cell death [44–46]...
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Báo cáo khoa học: "Does fluid loading influence measurements of intestinal permeability" docx
Ngày tải lên :
12/08/2014, 22:21
... osaccharides and disaccharides used in test of intestinal function and permeability Clin Chim Acta 1993, 221:115-125 Fink M: Clinical tests of gastrointestinal permeability that rely on the urinary ... after oral sugar administration in healthy volunteers However, further studies on intestinal and renal sugar handling in the assessment of gastrointestinal permeability are needed in critically ill ... intensive care Altered excretion of sugar probes by fluid loading would invalidate the measurement of intestinal permeability in patients requiring rapid intravenous infusions of fluids The data were...
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Evaluation of Meteosat-8 measurements using daily global solar radiation for two stations in Iraq
Ngày tải lên :
05/09/2013, 16:10
... summery of Heliosat-2 can be explain by the following steps; recording the ground albedo maps for each month, computing the cloud index, calculating the clear sky index, and finally estimating the ... satellite measurements, which play an effective role in evaluating the real values of solar radiation reaching earth surface and that which estimated using Meteosat-8 images By comparing the results of ... stations spring presented the highest RMSE and MAE and this due to the combined effect of the ozone, aerosols content and rain The lowest RMSE and MAE shown in summer which have arid and semi arid...
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Tài liệu MEASUREMENTS OF OUTDOOR AIR POLLUTION FROM SECONDHAND SMOKE ON THE UMBC CAMPUS pdf
Ngày tải lên :
17/02/2014, 22:20
... (particularly in children and individuals with asthma); and against alterations in lung tissue and structure and in respiratory tract defense mechanisms in all persons PM2.5 and PM3.5 (measured in this ... 14 involved simulating an area source, by locating smokers or smoldered cigarettes on chairs in a ring around the PAH and RSP monitors The ring radius was started at 1.5 meters, and increased in ... data relating to whether limitations on smoking in proximity to campus building entrances were justified Biographical Sketch of the Principal Investigator I am a biophysicist and an international...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
Ngày tải lên :
07/03/2014, 05:20
... radiation is induced by activation of Bax and Bak initiated by transcription upregulation of Fas and by phosphorylation ⁄ inactivation of Bcl-2 in a JNK-dependent manner An improved understanding of the ... were introduced into the cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations Quantification of cell death Apoptosis was investigated by Annexin V labeling ... Role of JNK in radiation-induced mitochondrial cell death M.-J Kim et al Fig Ionizing radiation induces expression of Fas and activation of caspases in human cervical cancer cells (A) Ionizing...
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Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx
Ngày tải lên :
08/03/2014, 08:20
... overproduction results in an increased level of free sterol (Fig 4B), and it would also be expected to increase the cycling of sterols by esterification and hydrolysis, resulting in an increase in intracellular ... similarly in yeast and in adrenal cells; in the latter, Adxp and not Adrp limits the activity of CYP11A1 and hence controls the extent of pregnenolone production [14] Protein partners involved in the ... absence of Adxp and Adrp [63] In conclusion, we have used steroid-producing S cerevisiae strains to study the different factors involved in the SCC of ergosta-5-eneol into pregnenolone, including...
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