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measurement of polyphenol oxidase activity

Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers

Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers

Sinh học

... Construction of T-DNA vectors carrying antisense PPO cDNAs In order to maximize the chances ofachieving ahigh level of antisense inhibition of PPO gene expression, we designed a series of antisense ... reducing patatin promoter activity" Ofthe large population of transgenic PPO lines generated, a small proportion of lines were not amenable to microtuber induction (
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Báo cáo khoa học: Oxidase activity of a flavin-dependent thymidylate synthase pot

Báo cáo khoa học: Oxidase activity of a flavin-dependent thymidylate synthase pot

Báo cáo khoa học

... velocity measurements in the absence of products [15,16] Therefore, the effect of NADP+ on initial velocities was examined by measuring the steady-state initial velocities of FDTS oxidase activity ... FDTS oxidase activity, and the addition of 400 lm CH2H4folate completely suppresses this activity under atmospheric O2 concentrations (210 lm) In order to investigate the nature of inhibition of ... that the presence of CH2H4folate slows the consumption of NADPH under aerobic conditions [8] In addition, the oxidase activity of FDTS calls for caution when studying the synthase activity under...
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Báo cáo khóa học: Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions ppt

Báo cáo khóa học: Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions ppt

Báo cáo khoa học

... exible a-helix of the protein structure, helix F (shown within elliptical traces) activity, we have investigated the effect of the Hofmeister series of anions on the activity of NADH oxidase from ... stabilization/destabilization of this part of NADH oxidase There are numerous reports on the effect of the Hofmeister series of salts on folding and stability of proteins [1618] and enzyme activity in both ... FEBS 2003 Anion eect on activity of NADH oxidase (Eur J Biochem 271) 53 Fig Dependence of the relative activity of NADH oxidase from T thermophilus on [urea], in the presence of M NaH2PO4 (j), Na2SO4...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc

Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc

Báo cáo khoa học

... [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide ... of sodium dithionite, the cycles of reoxidation of heme b initiated by O2 and Ó FEBS 2002 INT reductase activity of neutrophil oxidase (Eur J Biochem 269) 1249 Kinetics of inhibition of the oxidase ... independently of each other, depending on the presence of p67phox and p47phox [10,12] From these data it appeared that measurement of INT reductase could be taken as an index of the diaphorase activity of...
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báo cáo hóa học:

báo cáo hóa học: "Ambulatory measurement of knee motion and physical activity: preliminary evaluation of a smart activity monitor" pdf

Hóa học - Dầu khí

... physical activity[ 18] The Intelligent Device for Energy Expenditure and Activity system (IDEEA™, MiniSun, LLC), a microcomputer-based portable physical activity measurement device, allows detection of ... global DOF kinematics to DOF body segment kinematics [24] Body segment mass, center of mass, and mass moment of inertia were computed from regression equations using subject-specific anatomical measurements[23] ... connection and a software interface (provided by Minisun, LLC, the software also interprets the sensor output and determines activity type and other variables) With the addition of the electrogoniometers,...
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Báo cáo y học:

Báo cáo y học: "Measurement of endotoxin activity in critically ill patients using whole blood neutrophil dependent chemiluminescence" pps

Báo cáo khoa học

... abrogation of the endotoxindependent signal in the majority of samples Prevalence of endotoxaemia Using the EAA, 43 out of 74 patients (58%) had endotoxin levels greater than 50 pg/ml at the time of ... 1985, 13:818-829 25 Members of the American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference: American College of Chest Physicians/Society of Critical Care Medicine ... temperature and all samples were assayed within 30 of collection To assay levels of endotoxin, a 10 µl aliquot of whole blood was placed in each of three tubes containing luminol buffer (300 µl/tube)...
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Measurement of Color, Gloss, and Translucency of White Salted Noodles- Effects of Water Addition and Vacuum Mixing.pdf

Measurement of Color, Gloss, and Translucency of White Salted Noodles- Effects of Water Addition and Vacuum Mixing.pdf

Hóa học - Dầu khí

... levels of translucency of noodles made from the soft, low-FSV wheat flour and the soft, high-FSV wheat flour (9.25% protein) were 39.75 and 43.75%, respectively The mean levels of translucency of ... values of raw WSN sheets from soft and hard wheat flour samples Measurements of L*, a*, and b* usually give low standard deviations (SD) using the method described and this study gave mean SD of ... disks and strands from six WSN mixes of ASWN and gelatin, following standard procedures for sensory examination of foods (Standards Australia 1995) Measurement of Color in Experiments 2, 3, and...
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The measurement of spasticity

The measurement of spasticity

TOEFL - IELTS - TOEIC

... aspects of measurement scales are considered before going on to examine the attributes of instruments for the measurement of spasticity Level of measurement There are four distinct levels of measurement ... method of assessment – level of measurement The Tardieu method of assessment provides a composite measure of spasticity The quality of the muscle reaction (X) is a categorical level of measurement ... because of neurophysiological complexity and the lack of rigid definitions discussed above, there has been a variety of approaches to the measurement of spasticity While the majority of clinicians...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

Báo cáo khoa học

... site, which causes inhibition of cathepsin B endopeptidase activity and activation of its exopeptidase activity The dual activity of cathepsin B is a consequence of the occluding loop, a flexible ... molecular weights of 161.8, 28.2 and 13.2 kDa, respectively Regulation of cathepsin B activity The formation of the cathepsin B ⁄ cystatin C complex was seen as a shift of the elution peak of cathepsin ... both the early and late stages of protein breakdown The activity of cathepsin B is regulated in many ways, ranging from the pH of the environment to the presence of endogenous inhibitors (i.e...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Báo cáo khoa học

... 80% of the original activity after h The activity of N263D-Sendai decreased to 45% and 10% of the original after and h of incubation at pH 12, respectively N263D/A266Y-Sendai showed only 10% of ... retaining 60% of the original activity after h, and 30% after h The D266NALP I showed 40 and 20% of the original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- ... stability of D266N-ALP I cumulatively The double mutant enzyme showed 63% of the original activity after h of incubation However, the single mutation Tyr269Ala showed only 1% of the original activity...
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Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

Báo cáo khoa học

... overstimulation of b-cells by glucose could eventually lead to depletion of insulin stores, worsening of hyperglycemia, and finally deterioration of b-cell function [2] A large body of evidence shows ... dysfunction of b-cells, we first identified the source of ROS generated in response to d-glucose by determination of the effects on d-glucose-induced ROS levels of various inhibitors of ROS-generating ... 1B), suggesting deterioration of b-cell function Moreover, levels of ROS production in the pancreas of diabetic rats were significantly increased, confirming a state of oxidative stress (Fig 1C)...
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Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx

Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx

Báo cáo khoa học

... Stimulation of pNPPase activity of Na+ ⁄ K+-ATPase In the first part of this paper, we describe the effect of oligomycin with NaCl on pNPPase activity The ligand combination ... high-affinity K+-dependent pNPPase activity When the ratio of Na ⁄ Rb in the presence of oligomycin was 10, the binding of Rb+, a congener of K+, and pNPPase activity reached a maximal level and ... shown) PNPPase activity in the presence of oligomycin and NaCl The effect of oligomycin with NaCl on pNPPase activity was observed in the presence of 0–3 mm KCl (Fig 1) In the presence of mm KCl without...
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Measurement of the underlying event in the Drell–Yan process in proton–proton collisions at √ s =7 TeV pptx

Measurement of the underlying event in the Drell–Yan process in proton–proton collisions at √ s =7 TeV pptx

Tổ chức sự kiện

... the measurement of the UE activity The UE activity is studied as a function of the magnitude of the dimuon transverse momentum (pT = |pT ,1 + pT ,2 |) and as a function of M The dependence of ... dependence of the UE activity for DY events with small transverse momentum of the DY system This measurement separates the scale dependence of the UE activity from the ISR effect The universality of ... investigated as well The modeling of the ISR is studied by measuring the UE activity as a function of the transverse momentum of the DY system Finally, the dependence of the UE activity on ISR and FSR...
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Can’t Shake that Feeling: Event-Related fMRI Assessment of Sustained Amygdala Activity in Response to Emotional Information in Depressed Individuals pptx

Can’t Shake that Feeling: Event-Related fMRI Assessment of Sustained Amygdala Activity in Response to Emotional Information in Depressed Individuals pptx

Tổ chức sự kiện

... analog of time is on the x axis and activity is on the y axis The original network’s representation of negative information becomes active and quickly drops off (top left Affective Feature Unit activity ... between Ϫ.02 and SIMULATION OF A TRIAL Each phase of empirically administered trials was simulated for a number of epochs proportional to the time of each segment of the empirically administered ... before and after manipulation of variables related to depression To make predictions regarding the time course of amygdala activity in response to emotional stimuli, activity in the network’s valence...
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Báo cáo khoa học:

Báo cáo khoa học: "Automatic Measurement of Syntactic Development in Child Language" docx

Báo cáo khoa học

... 18 transcripts with a total of 11,704 words and a mean length of utterance of 2.9, and (B) 23 transcripts with a total of 40,819 words and a mean length of utterance of 7.0 5.2 Results Scores computed ... made more accurate with the knowledge of the expected levels of precision and recall of automatic assignment of specific GRs It is our intuition that in a number of cases it would be preferable to ... from zero to 112.1 Some of the language structures required in the computation of IPSyn scores (such as the presence of auxiliaries or modals) can be recognized with the use of existing child language...
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Báo cáo khoa học: Kinetic mechanisms of glycine oxidase fromBacillus subtilis docx

Báo cáo khoa học: Kinetic mechanisms of glycine oxidase fromBacillus subtilis docx

Báo cáo khoa học

... of the corresponding oxidases [8–10], particularly in light of the data presented here, will considerably expand our understanding of the evolution and the mode of functioning of this class of ... binding properties of glycine oxidase profoundly distinguish it from D-amino acid oxidase In the present study we investigated the kinetic properties of B subtilis glycine oxidase using three ... s (5) after mixing Inset: Course of anaerobic reduction of glycine oxidase followed in stopped-flow spectrophotometer Time courses of reaction of 10 lM glycine oxidase (recorded at 455 nm) after...
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Measurement of Organic Chemical Refractive Indexes Using an Optical Time-Domain Reflectometer docx

Measurement of Organic Chemical Refractive Indexes Using an Optical Time-Domain Reflectometer docx

Tự động hóa

... value (10 measurements of each sample) of each sample is reported in the paper For example, we measure the BSL level of water via the proposed OTDR-based fiber sensor under 10 time measurements ... average value of the 10 time measurements is about 35.0623 dB, as also illustrated in red dash line of Figure Figure Output BLS levels from the OTDR traces at 1,100 m SMF long under 10 time measurements ... deviation of the measurement is about 0.03033 By considering the laser power fluctuation and the OTDR only gives accuracy of the second digit after the decimal point We can also estimate that the measurement...
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Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf

Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf

Báo cáo khoa học

... Regulation of MMP activity E Hadler-Olsen et al prodomain of the enzyme remained intact [24] The specific activity of the proenzyme bound to the gelatincoated surface was approximately 10% of the ... the result of cleavage of a part of the PG core protein and at least a part of the C-terminal HPX domain of proMMP-9, leaving the hinge region bound to the enzyme [64] A large reduction of the HPX ... activation of inactive proenzymes The binding of MMPs to other macromolecules may also regulate the activity of the enzymes either through stabilization or through induction of autodegradation Enzyme activity...
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Electrical conductivity measurement of silicon wire prepared by CVD

Electrical conductivity measurement of silicon wire prepared by CVD

Vật lý

... value of SiO2 is 3.5 nm wider than that of Si Consequently, the SiNW of around 22 nm in diameter in Fig was estimated to consist of the core silicon crystal of 19 nm covered with silicon oxide of ... 0.491 nm of the lattice parameter for the (1 0) plane of quartz [21], q = 3.2 Â 10À19 C for the oxygen ion, and the average thickness of oxide of 12.5 nm as a result of the observation of the fractured ... temperature Fig is a schematic drawing of the electrical measurement of the SiNW covered with the silicon oxide layer The total resistance, R, is the sum of those of SiNW and silicon oxide as R ¼ R1...
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Báo cáo Y học: The S100A8/A9 protein as a partner for the cytosolic factors of NADPH oxidase activation in neutrophils doc

Báo cáo Y học: The S100A8/A9 protein as a partner for the cytosolic factors of NADPH oxidase activation in neutrophils doc

Báo cáo khoa học

... the effect of S100A8/A9 on the kinetics of oxidase activation The oxidase activity elicited in the cell-free system was the reflect of the extent of oxidase activation The experiment of Fig shows ... the effect of S100A8/A9 on the time course of oxidase activation For technical convenience, the time course of oxidase activation was analyzed by measuring the elicited oxidase activity of membrane-bound ... Inspection of the direct plots of the elicited oxidase activity indicated an enhancing effect of Fig Kinetic parameters of elicited NADPH oxidase after activation in the presence or absence of S100A8/A9...
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