measurement of dna damage in spermatozoa by tunel assay
Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... the synthesis of products greater than four nucleotides in length Murine DNA polymerase a-primase is absolutely inactive in initiating SV40 DNA replication in an assay consisting of purified enzymes ... liquid scintillation counting [46] Results Replication of JCV DNA in crude cell extracts with recombinant human and murine DNA polymerase a-primase Previously we reported the establishment of in vitro ... containing the SV40 origin of DNA replication (B) The JCV system uses JCV TAg and pJC389 DNA with the JCV origin The cell extracts were supplemented with 0.5 and 1.0 DNA polymerase units of the indicated...
- 8
- 326
- 0
in situ detection of dna damage, methods and protocols
... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage ... understanding of human disease One of the most widely used methods for detecting DNA damage in situ is TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining (1) TUNEL staining was initially ... digoxigenin-conjugated dUTP detected by staining with a FITC-conjugated anti-digoxigenin antibody or biotin-conjugated dUTP detected by staining with FITC-conjugated streptavidin Labeling DNA Damage...
- 279
- 384
- 0
Báo cáo khoa học: "Biological in-vivo measurement of dose distribution in patients’ lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate gland" ppsx
... therapy contains beams, whereas in SSIMRT, dose is distributed within 7-9 beams The distribution of low doses is broader in a larger volume in SSIMRT Using the gamma-H2AX stain to detect DNA- double ... also taken before radiation The protocol of staining gamma-H2AX by indirect immunofluorescence is published in many papers and its purpose for detecting DNA DSB validated [10, 11, 12, 13, 14 and ... Zwicker et al.: Biological in- vivo measurement of dose distribution in patients’ lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs step-and-shoot IMRT of the prostate gland Radiation...
- 8
- 352
- 0
Báo cáo y học: "Location of DNA damage by charge exchanging repair enzymes: effects of cooperativity on location time" doc
... from 2+ to 3+ upon binding to DNA [18] Binding is thus associated with the emission of an electron into the DNA, while upon receipt of an electron from DNA the MutYDNA binding complex is destabilized ... bound to DNA downstream of the error at the time of damage or by one that subsequently binds to DNA downstream of the error and then scans the DNA until it finds the damaged base pair In the regime ... the stretch of DNA ahead of it is thus error-free Presumably the electron received destabilizes the binding of MutY to this error-free stretch of DNA by changing the oxidation level of the [4Fe-4S]3+...
- 6
- 188
- 0
Measurement of OH Radicals in Aqueous Solution Produced by Atmosphericpressure LF Plasma Jet
... density in the solution as a function of treatment time as shown in Fig.7 The concentration of OH radicals in the cuvette almost linearly increases with increasing the time Besides trapping of OH by ... range of 6-11 Since TA (Aldrich) does not dissolve in acidic/neutral liquid, aqueous solution of TA was prepared by dissolving TA in the distilled water containing NaOH (Wako Pure Chemical Industries) ... shown in Fig.6 The fluorescence intensity corresponds to a time integrated OH radical concentration in the liquid As the time elapsed, the fluorescence intensity increased, indicating the increase...
- 5
- 486
- 0
Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf
... combination of DNA bending induced by DnaA and the cooperative interactions between DnaA monomers on DNA result in local topological tension that manifests itself by unwinding of this intrinsically ... and 1L8Q (DnaA) The AAA+ domains of both proteins are shown in cyan with ADP indicated in red The helix-turn-helix (HTH)-containing DNA binding domain of DnaA is in green and the winged helix ... position of the origin of replication in Pyrococcus, providing the first experimental proof of a localized origin of replication in the archaea [52] Interestingly, in a situation reminiscent of that in...
- 10
- 504
- 0
Báo cáo khoa học: "Automatic Measurement of Syntactic Development in Child Language" docx
... identification of certain GRs needs to be made more accurately We intend to annotate more in- domain training data for GR labeling, and we are currently investigating the use of other applicable GR parsing ... Annual Meeting of the Association for Computational Linguistics (pp 128-135) Dekang Lin 1998 Dependency-based evaluation of MINIPAR In Proceedings of the Workshop on the Evaluation of Parsing Systems ... accuracy of CP was 85.4% (a relative increase of about 100% in error) In their separate evaluation of CP, using 30 samples of typically developing children, Long and Channell (2001) found a 90.7% point-to-point...
- 8
- 580
- 0
Báo cáo Y học: Protein methylation as a marker of aspartate damage in glucose-6-phosphate dehydrogenase-deficient erythrocytes docx
... deamidation of sensitive asparagine residues [8] This protein is involved in the maintenance of erythrocyte shape and deformability, by stabilizing interactions between the spectrin–actin network and integral ... glycosylation of haemoglobin (glycation) has been shown to increase in the course of mismanaged hyperglycaemia in diabetes [27] Haemoglobin is a useful protein marker of this kind of damage, although ... Methyl esterification in intact erythrocytes was assayed by incubating them in the presence of [3H]methionine according to the in situ procedure (see Materials and methods) activity in the G6PD-deficient...
- 8
- 412
- 0
Tutorial: Sculpture of a Knight (in 35mm) by Gaël Goumon pdf
... sculpt the chain mail This is done line by line, making sure that the needle does not go too far into the GS I finish it by adding a small band of duro around the chain mail (made by rolling a small ... sheet of it by spreading it out thinly I cut some bands to make the belts, and apply those to the figurine, making sure not to damage the parts I have just finished Lastly, I add the loops of the ... arm I finish the right legging I then sculpt a base for the figure, upon an existing round plastic base I cut the wire at the bottom of the feet (leaving 0.4mm) and I insert the figurine in the...
- 7
- 586
- 0
measurement of metalcarbon nanotube contact resistance by adjusting contact length using laser ablation
... 23–29] In spite of the published work on CNTs, a clear understanding of the roles played by contact length, nanowire dimensions, and the contact metal still remains elusive In some instances, seemingly ... length of 1.0 k μm As the length of one of the contacts is shortened, the total resistance increases in a characteristic way according to the specified value of rCNT , the linear resistivity of the ... operating parameters The edge of the gap separating the two thin film electrodes is just barely visible in the bottom left-hand edge of the micrograph The figures in the inset show enlarged images of...
- 7
- 425
- 0
materials forum measurement of residual stresses in joints
... of pipe butt welds The procedure involved uniformly heating the cluster up to 600°C, holding for a period of hour and cooling back to room temperature Measurements of the movement of the lacing ... scans (1 and 4) This can be explained by rapid cooling in the last weld bead, particularly for scan Using understanding gained in work on neutron beam measurement of multi-bead welds18 it is probable ... residual stress measurements in a mining boom cluster before and after PWHT Experience with the use of incremental hole drilling technique for residual stresses measurements in the clusters is...
- 9
- 385
- 0
báo cáo hóa học:" Regression of orthotopic neuroblastoma in mice by targeting the endothelial and tumor cell compartments" pdf
... the upper right quarter of the counting grid mentioned above Immunohistochemical staining for DNA strand breaks (i.e cell death) was performed by the TUNEL assay using an "In Situ Cell Death Detection ... of murine origin and human tumor cells were affected by treatment with CHS 828 Thus, we believe that the current administration of CHS 828 represents a dual targeting approach involving the inhibition ... analyzed by DF and FA, except for CgA data which was analyzed by MS RC contributed with data interpretation and drafting of the manuscript written by DF and FA EL and MS provided input in writing of...
- 11
- 593
- 0
Báo cáo sinh học: "Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke" pot
... defined as neuroserpin decrease All determinations were carried out in a laboratory blind to the clinical outcome and neuroimaging findings Endpoints The primary endpoint was the decrease of ... Neuroserpin was used as a continuous variable since there was a linearity of the odds ratios for outcome The influence of neuroserpin decrease at 24 h on serum levels of molecular markers of brain injury ... http://www.translational-medicine.com/content/9/1/58 our group obtained after investigating neuroserpin effects using an in vitro model of brain ischemia [40] The information reported here regarding biomarkers...
- 7
- 541
- 0
Báo cáo hóa học: " Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy" pot
... Raman scattering of a cylindrical phage Thus a continuum approach, when used cautiously, is a method of choice due to its relative simplicity and ease of interpretation In building a continuum model, ... is a linear combination of Bessel functions of the 1st and 2nd kinds, and the angular dependence is sinusoidal being proportional to sin(nθ) and cos(nθ) where n is an integer, n = [0,∞] Since ... Diagrams of the structure of M13 phage Diagrams of the structure of M13 phage (a) A molecular model containing 10 α-helix coat proteins forming a cone of the structure (b) A cross section of the...
- 11
- 315
- 0
báo cáo hóa học:" Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke" pdf
... defined as neuroserpin decrease All determinations were carried out in a laboratory blind to the clinical outcome and neuroimaging findings Endpoints The primary endpoint was the decrease of ... Neuroserpin was used as a continuous variable since there was a linearity of the odds ratios for outcome The influence of neuroserpin decrease at 24 h on serum levels of molecular markers of brain injury ... http://www.translational-medicine.com/content/9/1/58 our group obtained after investigating neuroserpin effects using an in vitro model of brain ischemia [40] The information reported here regarding biomarkers...
- 7
- 404
- 0
báo cáo hóa học: " Psychometric validation of the revised SCOPADiary Card: expanding the measurement of nonmotor symptoms in parkinson’s disease" potx
... groups of PD patients with off-time: feelings of anxiety, mood swings, loss of interest, fatigue and autonomic or gastrointestinal symptoms (such as excessive sweating, salivation, and incontinence) ... items (feelings of exhaustion or fatigue, difficulty concentrating or remembering, feelings of anxiety or panic, unexplained pains, difficulty swallowing, frequent or urgent urination, sweating too ... validity of the Revised SCOPA-DC in a U.S population Page of 11 Literature review The first step in the process of refining the SCOPADC was to gain a sound understanding of the full spectrum of PD...
- 11
- 650
- 0
báo cáo hóa học:" Quantitation of glucose uptake in tumors by dynamic FDG-PET has less glucose bias and lower variability when adjusted for partial saturation of glucose transport" potx
... in nu/nu 537−MEL in nu/nu H2122 in nu/nu FaDu in C.B−17 scid H292 in C.B−17 scid BT474M1 in beige−scid nude HCT116 in nu/nu HCT116 in nu/nu A2058 in nu/nu A375 in nu/nu A2058 in nu/nu A2058 in ... reproducibility measurements according to Equation were conducted using this instrument in 20 mice and showed a coefficient of variation of 3.7% Image analysis Regions of interest [ROIs] were drawn using ... presented in this paper were computed using Equation 4, and group mean data were calculated by sample averaging the results for individual animals within a given study Estimation of KM by minimizing...
- 31
- 470
- 0
The Project Gutenberg EBook of Creative Impulse in Industry, by Helen Marot potx
... contain an increment of value in profits to the owners of wealth It was during the Guild period that business came to value workmanship because it contained that increment In spite of business interest, ... colleges but from industry The institution of industry, rather than the institution of education, dominates thought in industrial education courses It is the institution of industry as it has ... arduous way of work but of capture The scheme of capture is a scheme whereby you may get something for (doing) nothing; nothing as nearly as possible in the way of fabrication of goods; something for...
- 306
- 360
- 0
The Project Gutenberg EBook of Forging Ahead in Business, by Various pdf
... and illuminate his reading of the Text by a series of Modern Business Talks To bring him into touch with the ideas and methods of some of the foremost business and professional men in the country ... Marketing and Merchandising Salesmanship and Sales Management Advertising Principles Office Administration Accounting Principles Credit and Collections Business Correspondence Cost Finding Advertising ... portion of their spare time in reading and thinking about business problems The organization The Modern Business Course and Service is conducted by an organization made up of business and professional...
- 374
- 2,477
- 0
The Project Gutenberg EBook of Explanation of Terms Used in Entomology, by John. B. Smith potx
... dusky tinge Albinic: of the character of an albino Albinism: that condition in which there is an absence of color or a whitening in a form usually colored Albino: a colorless individual of a species ... functional legs in the adult stage Abdominal: belonging or pertaining to the abdomen Abdominal feet: see pro-legs Abdominal groove : the concave lobe of the inner margin of secondaries enveloping the ... tapering to a long point Acupunctate : a surface with fine punctures as if made with a needle Acutangulate : forming, or meeting in an acute angle Acute : pointed: terminating in or forming less...
- 912
- 285
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008