matrix cell adhesion molecules and proteolysis in glioma invasion and tumorigenicity
Báo cáo khoa học: Extraenzymatic functions of the dipeptidyl peptidase IV-related proteins DP8 and DP9 in cell adhesion, migration and apoptosis doc
Ngày tải lên :
23/03/2014, 11:20
... TIMP2 and b-catenin can in uence cell adhesion and apoptosis [23,28] DDR1 is an integrin-independent cell adhesion molecule DPIV reduces cell adhesion by dephosphorylating p38 MAP kinase and b1-integrin ... DPIV and FAP in HEK 293T and LX-2 cells have further established these roles in cell ECM interactions [12] Cell adhesion is crucial in monolayer wound healing and cell migration Therefore, the adhesion ... overexpression in melanoma and non-small cell lung carcinoma cell lines inhibits the processes of tumor progression, including anchorage-independent growth, cell migration and tumorigenicity...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
Ngày tải lên :
13/08/2014, 05:21
... NF-κB and AP1 These findings suggest that Tax protein could induce the transcription of cytokines, chemokines and cell adhesion molecules in lung epithelial cells in a manner similar to that in ... immunohistochemical staining showed definite brownish staining for Tax protein in the cytoplasm of epithelial cells (A and D), and infiltrated lymphocytes (B and E) and macrophages (C and F) HTLV-I can ... Methods Cell culture and in vitro HTLV-I infection Human A549, a type II alveolar epithelial cell line, and NCI-H292, a tracheal epithelial cell line, were maintained in RPMI 1640 containing 10%...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... overload in hepatic cells in clinical situations in vivo might cause changes in lipid metabolism and consequently in lipid binding molecules such as CD1d CD1d upregulation in iron-loaded HepG2 cells ... opposite changes in CD71 and intracellular ferritin in HepG2 cells grown in iron-rich media were observed regardless of the time in culture Under these conditions HepG2 cells showed intracellular iron ... antibody (thin lines) or with rabbit Igs (thick lines) as first step in permeabilized cells (C) Histograms show ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
Ngày tải lên :
07/03/2014, 16:20
... containing CLP and CLAP with a phosphoprotein stain did not reveal phosphate additions to these proteins Only lanes in the gel containing the known phosphoproteins ovalbumin, b-casein and pepsin ... fasciclin domains in CLAP with other fasciclin I containing proteins is clear (Fig 9) Using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) to identify related proteins, a putative cell adhesion protein ... 271) binding domains, so the presence of a GTP/ATP binding region in CLAP may indicate an energy dependent mechanism for pro-CLAP processing or cathepsin L docking and stabilization not found in...
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Báo cáo khoa học: Acetylcholinesterase in cell adhesion, neurite growth and network formation pot
Ngày tải lên :
23/03/2014, 07:20
... globular domain IV of the b1 chain of laminin [23] In vitro binding studies similarly indicated binding of acetylcholinesterase to laminin-1 [24], which was inhibited by peripheral anionic site inhibitors ... Acetylcholinesterase interaction with laminin-1 An adhesive protein is defined by its localization on the cell surface and by the fact that it is involved in binding to other cells or to the extracellular ... inhibitors (fasciculin, propidium and gallamine) and an anti-acetylcholinesterase mAb Increasing the ionic strength, or decreasing the pH decreased this binding [23,24], indicating a dominant role for...
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Báo cáo sinh học: " Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow" ppt
Ngày tải lên :
18/06/2014, 22:20
... epithelial and non-epithelial origins, is promoted by inflammation [6,10] Proinflammatory cytokines released by cancer cells [11] and tumor-activated BM stromal cells [12] increase cancer cell adhesion ... to bone cells [13] and bone resorption [14,15] In addition, PGE2 induces VEGF [16] and osteoclast formation [17] in preclinical models of bone-metastasizing carcinomas, suggesting that inflammation ... COX-2 in the regulation of murine B16 and human A375 melanoma cell adhesion and proliferation in response to primary cultured murine and human BM stromal cell (BMSC)conditioned media (CM) in vitro...
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Báo cáo y học: " Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung" pot
Ngày tải lên :
12/08/2014, 14:20
... BALT T cells and B cells for the expression of L-selectin, which binds to PNAd; α4 integrin, which is a subunit of α4β1 integrin (which binds mainly to VCAM-1) and α4β7 integrin (which binds to ... T cells (CD3+) and T cells expressing LFA-1, α4 integrin or L-selectin in BALT from randomly selected patients, and calculated the percentage of T cells that expressed each adhesion molecule In ... tissues, BALT T cells differ from B cells in adhesion molecule expression Lselectin, which binds to PNAd, was expressed on 20% of T cells and almost all B cells in BALT α4 integrin, which is a...
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Báo cáo khoa học: "Changes in some Blood Micronutrients, Leukocytes and Neutrophil Expression of Adhesion Molecules in Periparturient Dairy Cows" ppt
Ngày tải lên :
12/08/2014, 15:20
... immunostaining of CD18 and CD62L adhesion molecules, and for total and differential white blood cell counts Leukocyte counts Total and differential leukocyte counts were determined within h using a Cell- DynR3500 ... collected in the morning, using Vacutainer® tubes (Becton Dickinson Vacutainer Systems, Meylan, France), one month before estimated calving, at calving (within 24 hours after calving) and one month ... significant increase in the numbers of WBC at calving This was mainly due to an increase in the numbers of circulating neutrophils, and to a less extent, an increase in monocytes At calving, the...
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Báo cáo y học: "The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands. They are transmembrane α" ppsx
Ngày tải lên :
14/08/2014, 07:21
... Table Ligand-binding specificities of human integrins Integrins Ligands α1β1 Laminin, collagen α2β1 Laminin, collagen, thrombospondin, E-cadherin, tenascin α3β1 Laminin, thrombospondin, uPAR ... ligand specificity, the mammalian integrins can be broadly grouped into laminin-binding integrins (α1β1, α2β1, α3β1, α6β1, α7β1, and α6β4), collagen-binding integrins (α1β1, α2β1, α3β1, α10β1, and ... integrins, the I domains play a central role in ligand binding and intercellular adhesion Integrin binding among invertebrate species is less well studied; RGD sequences have been found in species...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
Ngày tải lên :
25/10/2013, 21:20
... containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing the binding of E2F and ... and cell cycle control Cell 81:323–330 Welch PJ, Wang JY (1993) A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl tyrosine kinase in the cell cycle Cell ... while increased apoptosis was only observed in follicular cells lacking both pRb and p107 (Ruiz et al 2004) Interesting observations were made in cells lacking pRb in the retina Somatic inactivation...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
Ngày tải lên :
18/02/2014, 16:20
... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ... N-Terminal domain; B, Rio1 family-specific domain (R-domain); CK, serine-rich acidic domain (pos 402–435, CK2 domain); and K, lysine-rich C-terminal domain (K domain, pos 436–484) (B) N- and C-terminally ... C (2002) Joining the cell survival squad: an emerging role for protein kinase CK2 Trends Cell Biol 12, 226–230 Meggio F & Pinna LA (2003) One-thousand -and- one substrates of protein kinase CK2?...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
Ngày tải lên :
19/02/2014, 13:20
... Thus, in terms of inhibition of cell adhesion, cVY has more potential since it may directly interrupt the binding mechanism of CD2–CD58 Inhibition of cell adhesion involves interrupting the interaction ... modulate cell- adhesion by binding to CD58, hence inhibiting CD2–CD58 interaction Therefore, docking studies of peptides to CD58 protein were carried out in order to understand peptide–protein interactions ... particularly in the case of linear peptide lVY This peptide showed enhancement in the antibody binding assay, whereas in celladhesion inhibition assay, the peptide inhibited cell- cell adhesion While...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
Ngày tải lên :
07/03/2014, 16:20
... over-expression of the geminin protein differs between cell lines, resulting in gross differences in relative amounts of Cdt1 and its inhibitor in different cell lines It would be interesting to investigate ... HeLa cells (marked HeLa) was run alongside known amounts of recombinant full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) ... blotting with commercial antibodies against geminin showed that geminin protein levels are also increased in cancer cell lines, while different cancer cell lines appear to over-express geminin...
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Báo cáo khoa học: Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae Octavian Stephan and Christian Koch ppt
Ngày tải lên :
23/03/2014, 05:22
... of Sin3 and or Rpd3, rather than by regulating Sin3 ⁄ Rpd3 directly A good candidate for a factor regulating Sin3 ⁄ Rpd3 binding is Stb1, because it binds to both Swi6 and Sin3 [20,21,32,34] In ... Stb1 was originally identified as an interacting protein of Sin3 in two-hybrid assays (Stb1 for Sin three binding) [34] STB1 transcription was shown to be cell- cycle regulated and peaks in late G1 ... [44] and genome-wide binding studies for Rpd3 [39] showed that genes involved in cell growth and cell- cycle control, including the G1-specific gene PCL1, are targeted by the Rpd3 deacetylase In...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
Ngày tải lên :
23/03/2014, 07:20
... proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and corn root tip cells [28,29] Cationic oligopeptides such as polyarginine ... CPPs and their cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are only few reports on translocation of CPPs in plant cells ... complex in the cellular milieu and its further fate in plant cells Future investigations will focus on answer2410 A Chugh and F Eudes ing questions such as how the combined use of cell permeabilizing...
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Báo cáo khoa học: Cleavage of focal adhesion proteins and PKCd during lovastatin-induced apoptosis in spontaneously immortalized rat brain neuroblasts ppt
Ngày tải lên :
23/03/2014, 11:20
... protein aggregates linking actin filaments to the cytoplasmic domain of integrins Maintenance of cell matrix contact is an important cell survival factor and the loss of cell matrix and cell cell ... [16] Focal adhesion complexes consist of integrin proteins, the tyrosine kinases Src and focal adhesion kinase (FAK), actin-binding structural proteins, and the adaptor proteins paxillin and Crk-associated ... (CDKN1A)-activated kinase (PAK2) [28], MAPK kinase kinase (MEKK-1) [29], MAPK kinase kinase kinase (Raf-1), protein kinase B (Akt) [30,31] and protein kinase C (PKC) [32] PKC is a family of serine ⁄ threonine...
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Báo cáo sinh học: "Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function" potx
Ngày tải lên :
18/06/2014, 19:20
... performance in cell based assays such as ELIspot and intracellular cytokine staining assays [27] Delaying processing of whole blood by hours also impaired the response of antigen-presenting cells to ... to microbial antigens in both HIV-infected and healthy donors [11,12] Single cell- based techniques such as ELIspot assays [13-15], intracellular cytokine staining [16-19], and HLA-specific multimeric ... temperatures on maintaining internal temperatures within the range of 15-35°C was tested on the newly designed JVI shipping container The shipping container was placed at 45°C (Figure 7) and for 45...
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Báo cáo khoa học: "Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis" doc
Ngày tải lên :
07/08/2014, 18:20
... role in worm expulsion, then the introduction of migrating L3 into a hostile intestinal environment in primed KO and WT mice would most likely result in a patent infection in the former but not in ... priming dose On day 30 post priming, each animal in the challenge and control groups was infected with 300 lungrecovered L3 contained in 200 µl PBS and introduced directly into the stomach using ... defense against gastrointestinal nematodes: responses of mucosal mast cells and mouse mast cell protease during primary Strongyloides venezuelensis infection in FcRγ-knockout mice Infec Immun...
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