matrix adhesion molecules cell surface markers and related proteins
Báo cáo y học: "The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands. They are transmembrane α" ppsx
Ngày tải lên :
14/08/2014, 07:21
... kinase/cSrc, and the small GTPases Ras and Rho) and adaptors (for example, Cas/Crk and paxillin) that assemble within dynamic adhesion structures, including focal adhesions that bind cells to the extracellular ... between extracellular contacts (other cells or the extracellular matrix) and the actin microfilaments of the cytoskeleton, whose behavior integrins also regulate and modulate Many different proteins ... extracellular matrix In mammals, some integrins are limited to certain cell types or tissues: αIIbβ3 to platelets; α6β4 to keratinocytes; αEβ7 to T cells, dendritic cells and mast cells in mucosal...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
Ngày tải lên :
09/08/2014, 01:23
... of surface markers [4,8,23,24] MPCs express a large number of adhesion molecules such as activated leukocyte cell adhesion molecule (CD166), the hyaluronate receptor (CD44) and intercellular adhesion ... CD54, CD90 and CD166 as positive markers, and CD45, CD133/-1 and -2 as negative markers to eliminate haematopoietic and endothelial cells (Table 2) The expression of progenitor typical markers varied ... MPC related cell surface markers In addition, Jones and coworkers [22] recently identified cells with characteristics of MPCs in synovial fluid of osteoarthritic patients using FACS analysis and...
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Báo cáo y học: " Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes" docx
Ngày tải lên :
12/08/2014, 23:23
... downregulates cell surface CXCR4, CCR5 and CD4 expression on Rev-CEM cells The Rev-CEM cell line was derived by transducing the Rev and Tat dependent GFP vector pNL-RRE(SA) [18] into CEM-SS cells, resulting ... of cell surface CD4 by Nef in primary CD4+ T-cells by unintegrated DNA is well established [8,14] We now show that Nef derived from unintegrated DNA can also downregulate cell surface CXCR4 and ... downregulation of each of cell surface CD4 and the CXCR4 [34,35] and CCR5 [36] coreceptors has the benefit of restricting superinfection This may protect the virus within the cell from cellular toxicities...
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Báo cáo khoa học: " Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis" ppt
Ngày tải lên :
13/08/2014, 10:20
... between host and infecting microorganism, including bacterial recognition, cell activation and transmigration, phagocytosis and destruction of the pathogen [3-6] Bacterial recognition and cellular ... neutrophil and monocyte activation through ROS production, and correlated cell activation and organ dysfunction measured by the sepsis-associated organ failure assessment (SOFA) score [26] Materials and ... gated based on FSC versus SSC (forward scatter and side scatter) parameters and CD66b+ CD14- stained cells Surface markers were analyzed in histograms and expressed as the geometric mean fluorescence...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
Ngày tải lên :
21/02/2014, 03:20
... easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein or with lM ¯uorescein Tat peptide and ... RESULTS Uptake and cellular localization of ¯uorescently labeled Tat peptides Cellular uptake of the full-length Tat protein fused to GFP and/ or GST involves an interaction with cell surface HS proteoglycans ... was observed for all cell lines (Fig 2A) In order to minimize cell handling prior to FACS analysis, no ®xation step was included Avoiding cell ®xation and working on living cells eliminates potential...
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Báo cáo khoa học: Extraenzymatic functions of the dipeptidyl peptidase IV-related proteins DP8 and DP9 in cell adhesion, migration and apoptosis doc
Ngày tải lên :
23/03/2014, 11:20
... b-catenin can influence cell adhesion and apoptosis [23,28] DDR1 is an integrin-independent cell adhesion molecule DPIV reduces cell adhesion by dephosphorylating p38 MAP kinase and b1-integrin [29], ... sequence relatedness, DP8 and DP9 exert these differences in their cellular effects Therefore, these two proteins are likely to have different functions and ligands These data indicate that DP8 and ... further established these roles in cell ECM interactions [12] Cell adhesion is crucial in monolayer wound healing and cell migration Therefore, the adhesion defect of cells overexpressing DP8 or DP9...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
Ngày tải lên :
13/08/2014, 05:21
... IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells ... chemokines and cell adhesion molecule in A549 cells cocultured with MT2 cells A549 cells were cocultured with MMC-treated MT-2 or CCRF-CEM cells At and days after cocultivation, A549 cells were ... several cellular genes correlated with that of Tax MT-2 cells expressed IFN-γ and IL-10 mRNA, but not IL-1β and IL-8 mRNA However, A549 cells cocultured with MT-2 cells expressed IL-1β and IL-8...
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Molecular mechanisms of mechanosensing at cell cell and cell matrix adhesion 2
Ngày tải lên :
09/09/2015, 08:13
... between adhesion proteins [44] Mechanical force is a critical factor that couples tightly with the formation and dynamics of cell -matrix adhesions Most cell -matrix adhesions, such as focal complex and ... conformation changes At subcellular level, vinculin plays important roles in regulating cell -matrix and cell- cell adhesions Cells lacking vinculin develop less stable focal adhesions and migrate faster ... related proteins at cell- adhesions As cell adhesions play a pivotal role in the proper function of cells, misregulation of cell adhesions often leads to severe pathological consequences Altered cell- cell...
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Clinical significance of cancer stem cell markers and other related proteins in colorectal carcinomas biological and methodological considerations
Ngày tải lên :
03/10/2015, 20:57
... inherent stem cell capacity to self-renew and differentiate In this thesis, the molecular and clinical significance of cancer stemcell markers and its related proteins in colorectal cancers were investigated ... of Cancer Stem -Cell Markers 3.2.2 Selection of Protein Markers with Discriminatory Power for Prognostic and Predictive Significance 3.2.3 Associations between Markers Expression and Clinicopathological ... image scanning v and analysis platform to examine the immunohistochemical expression of a panel of nine markers, of which three are cancer stem cell related proteins In addition, BRAF and KRAS mutation...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
Ngày tải lên :
21/02/2014, 03:20
... identical in untreated and bFGF-treated Sertoli cell cultures (2525 257 and 2538 246 ng per well in three different cell cultures) Cell surface HSPG are bFGF partners during Sertoli cell postnatal ... Sertoli cells are bFGF producing cells [2,33] and are the target of this growth factor as bFGF inhibits FSH-induced estradiol synthesis ([5], and our results) HSPG and especially glypicans and syndecans ... In conclusion, bFGF requires cell surface HSPG for the inhibition of steroidogenesis in developing Sertoli cells Cell surface HSPG are bFGF targets during Sertoli cell postnatal development As...
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Signaling at the Cell Surface in the Circulatory and Ventilatory Systems docx
Ngày tải lên :
05/03/2014, 22:21
... Signal Processing Cell communications with other cells and the extracellular matrix involve cellsurface proteins, such as receptors, cell adhesion molecules, ion carriers, and plasmalemmal constituents ... (Chaps to 11) and intracellular (Chap 6) receptors, and incorporated adaptors; as well as (2) ion carriers (Chaps and 3); (3) cell surface transporters (Chaps and 5); (4) cell adhesion molecules ... responsible for dynamical interactions between cells and their environment Cell- surface integrins recognize and bind extracellular matrix proteins and control the organization of the cytoskeleton,...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
Ngày tải lên :
06/03/2014, 22:21
... (MHC) class I molecules and the protein is organized into a1, a2 and a3 structural domains that resemble those described for MHC class I and related proteins [4,16] The N-terminal a1 and a2 domains ... and methods Cell culture HEK293T and HuTu80 cells were obtained from the American Type Culture Collection and cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum and ... indicated Cells were immunostained with anti-HA and anti-BiP Ig, and processed for fluorescence microscopy Co-localization masks were created as described in Materials and methods, and represent...
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Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
Ngày tải lên :
06/03/2014, 22:21
... Functional classification of scaffold proteins L Buday and P Tompa Table Scaffold proteins and their kin Representatives of the four categories of scaffold proteins and their relatives are presented ... activation and cell death [19] Classification of scaffolds and its limitations The functions of the above four closely related categories of signaling regulatory proteins show significant similarities and ... regulating more proteins in complex ways (scaffolds ⁄ anchoring proteins) and proteins specialized to initiate signaling cascades by localizing partners at the cell membrane (docking proteins) This...
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Báo cáo hóa học: " The role of single N-glycans in proteolytic processing and cell surface transport of the Lassa virus glycoprotein GP-C" ppt
Ngày tải lên :
20/06/2014, 01:20
... transported to the cell surface and remain mannose-rich Materials and methods N-glycans play not only an important role in folding and intracellular transport of viral glycoproteins but also are ... cells were surface labelled with biotin Following cell lysis and immunoprecipitation using α-GP-2, samples were subjected to SDS-PAGE and blotted to nitrocellulose Surface biotin-labelled proteins ... attachment Cell surface transport of GP N-glycosylation mutants N-glycosylation has long been known to be essential for correct folding and intracellular transport of viral glyco- proteins, as...
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Báo cáo hóa học: "Membrane Surface Nanostructures and Adhesion Property of T Lymphocytes Exploited by AFM" ppt
Ngày tải lên :
21/06/2014, 20:20
... results of surface adhesion force, showing the fixed cells have a greater adhesion force than unfixed cells h Comparison of adhesion force of control groups and testing groups, indicating the adhesion ... changes of average roughness (Ra) and particle mean height of surface nanostructure, cell height, cell diameter and cell volume The results demonstrate that cell volume double increases from ... force, stiffness, and others) of cells will provide fundamental insights into cellular structures and biology functions [14] However, the variation of membrane surface nanostructures and nano-mechanical...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
Ngày tải lên :
12/08/2014, 04:21
... virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... enteroviruses and their target cells RT-PCR is a rapid and sensitive method suitable for attachment studies and allows the use of crude virus containing extracts as well as limited amounts of cells and ... Results and Discussion Cells and viruses HeLa-SoH (provided by M Rovainen, Helsinki, Finland), CHO, CHO-CAR and CHO-DAF [30,31] cells were main- Comparison of purified and unpurified viruses...
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báo cáo khoa học: " Constitutive Expressor of Pathogenesis-Related Genes5 affects cell wall biogenesis and trichome development" potx
Ngày tải lên :
12/08/2014, 05:20
... senescence [4], and cell proliferation and cell death [3] The work presented here identifies a previously unrecognized cell wall defect in cpr5 mutants, resulting in a deficit of paracrystalline cellulose ... pathogenesis -related genes1, nonexpressor of PR genes1 (npr1), accelerated cell death2 (acd2), and accelerated cell death6 (acd6), and these mutants appeared to have normal birefringent trichome cell ... cyclin-dependent kinase inhibitor ICK1/KRP1 in single-celled Arabidopsis trichomes reduces endoreduplication and cell size and induces cell death Plant Cell 2003, 15(2):303-315 Somerville C, Bauer S,...
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Báo cáo y học: " Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung" pot
Ngày tải lên :
12/08/2014, 14:20
... We counted CD4+CD45RA+ cells (naive CD4+ T cells) and CD4+CD45RO+ cells (memory CD4+ T cells) in BALT from randomly selected patients, and calculated the percent of CD4+ T cells with naive or memory ... http://respiratory-research.com/content/10/1/97 total T cells (CD3+) and T cells expressing LFA-1, α4 integrin or L-selectin in BALT from randomly selected patients, and calculated the percentage of T cells that expressed each adhesion molecule ... mAbs against CD19 and CD3 (A), CD4 and CD8 (B), CD4 and CD45RO (C), and CD4 and CD45RA (D) L is in the airway lumen of the bronchus (A) B cell follicle (CD19+, red) surrounded by T cells (CD3+, green)...
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Báo cáo khoa học: "Changes in some Blood Micronutrients, Leukocytes and Neutrophil Expression of Adhesion Molecules in Periparturient Dairy Cows" ppt
Ngày tải lên :
12/08/2014, 15:20
... of CD18 and CD62L adhesion molecules, and for total and differential white blood cell counts Leukocyte counts Total and differential leukocyte counts were determined within h using a Cell- DynR3500 ... nutrients and leukocytes, and the proportions and MFI for the neutrophil adhesion molecules were done using the General Linear Model (SAS Institute Inc., Cary, NC, USA) The effects of cow and period ... commercial mineral and vitamin mix Samples of hay, concentrate and silage were frozen at –20ºC and analysed for contents of vitamins, minerals and trace elements The total daily requirements and allotments...
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Báo cáo y học: " The predictive role of serum and bronchoalveolar lavage cytokines and adhesion molecules for acute respiratory distress syndrome development and outcome" pptx
Ngày tải lên :
12/08/2014, 18:20
... lung adenocarcinoma cell line, NCI-H441, and human fetal lung explants H441 cells resemble bronchiolar epithelial Clara cells in phenotype and produce both SP-A and SP-B mRNA and protein [14] The ... vehicle or insulin (2.5 µg/ml) for an additional 1, 4, and 24 hours The cells were then rinsed and trypsinized, and nuclei from control and treated cells were harvested The transcription elongation ... MAPK and p70 S6 kinase in cells incubated in the presence of insulin (2.5 µg/ml), PD98059 (10 µM) and/ or rapamycin (50 nM) Cells were harvested and immunoblotted for phosphorylated p44/42 MAPK and...
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