manuals mathematics teaching methods
Safe carrying methods
... section 2 they will be in breach of the law and liable for a fine of $25 000. Safe carrying methods Carrying heavy objects can put a strain on your back, arms, shoulders and knees because ... that are too heavy for you to lift or carry. In these cases, you may need to use some of these methods: • Work with someone to carry the load.• Use a trolley.• Use a forklift or trolley jack...
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MOB Subject 5 –Access methods Static and dynamic access techniques
... - MOB Mobile Internet and Surrounding1/12 Baey, Fladenmuller – Subject 5MOB Subject 5 –Access methods Static and dynamic access techniques1. Static access techniques1.1 Space-division multiple-access...
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Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
... Biochem. Mol.Biol. 27, 5792. Methods in Molecular BiologyTMHUMANA PRESSGlycoprotein Methods and ProtocolsEdited byAnthony P. CorfieldVOLUME 125The Mucins Methods in Molecular BiologyTMHUMANA ... separation between mucinsIsolation of Large Gel-Forming Mucins 33From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... analyses.3.6. Analysis of Mucins Methods for the detection and analysis of mucins are dealt with in other chapters inthis volume. However, three principally different methods are available: solutionassays...
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Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
... nonsulfated mucins remainunstained.Histological Methods for Detection of Mucin 2929From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. ... tract.Histological Methods for Detection of Mucin 393. Alternatively, prepared adhesive slides such as Superfrost Plus®/(Menzel-Gläser,Braunschweig, Germany) may be purchased.4. There are many other methods ... the demonstration of mucin was mucicarmine(4), but this stain has now been largely supplanted by methods based on more strictlyhistochemical approaches that utilize a specific chemical reaction...
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Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
... Mucins can be detected by chemical methods within acrylamideDetection and Quantitation of Mucins 4545From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited ... mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods andProtocols: ... (6).References1. Walsh, M. D., Jass, J. R. (2000) Histologically-based methods for detection of mucin,in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana,Totowa, NJ.2....
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Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
... wrapping them in a 1–cm2sand-GI Adherent Mucous Gel Barrier 5757From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... mucouslayer existed over the gastric mucosal surface (13,14). However, a variety of different methods (discussed subsequently) have now demonstrated unequivocally that a thick(100–200 µm), ... consisting primarily of a fibrin gel and necrotic cells with some mucin stain-ing (15).Original methods for measuring adherent mucous thickness in situ were based on(1) differences in refractive...
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Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
... PhosphorImager as au/A260nm.Biosynthesis and Secretion of Mucin 6565From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... is isolatedaccording to the manufacturer. Measure the following components:68 Dekker et al.3. Methods 3.1. General Assays for Quantitation of Mucin (see Notes 1–3)Each of these six detailed...
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Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
... there are few separation techniques available to use with these77From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... Biotechnol. 5, 171–176.10. Thornton, D. J., Howard, M., Devine, P. L. and Sheehan, J. K. (1995) Methods for separa-tion and deglycosylation of mucin subunits. Anal. Biochem. 227, 162–167.Separation ... monomersgenerated after reduction (Mr= 2–3 × 106) are amenable to conventional biochemicalseparation methods. We describe a separation protocol based on anion exchange chro-matography to fractionate...
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Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
... 5).Heterogeneity and Size Distribution of Gel-Forming Mucins 8787From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... chapter describes only methods for assessing the heterogeneity of mucinpopulations with regard to mass and size utilizing rate zonal centrifugation and electronmicroscopy. Other methods for the absolute ... 96 Sheehan and Thornton12. Koller, T., Harford, A. G., Lee, Y. K., and Beer, M. (1969) New methods for the prepara-tion of nucleic acid molecules for electron microscopy. Micron. 1, 110–118.13....
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Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
... code as defined in Fig. 1.Amino Acid Analysis of Mucins 113113From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... Mobile phase B: 15% (v/v) methanol in water.6. Mobile phase C: 90% (v/v) acetonitrile in water.3. Methods 3.1. Sample Preparation and Hydrolysis1. Add an aliquot of a solution of a sample of mucin...
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Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
... Sequencing Glycopeptides 121121From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for theseparation of PTH-glycosylated amino acids on a modified Beckman LF3600sequenator (12). These methods were developed for two reasons: ... withhigh molecular weight, heavily glycosylated domains such as those found in mucins.The two methods for determining the sites of glycosylation in proteins are massspectrometry and Edman sequencing....
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Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf
... RTrityl CSynthetic Peptides for Antimucin Antibodies 129129From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press ... cannot be controlled or simply that short peptides do not adherewell to polystyrene plates. Several methods of peptide modification have been utilizedto overcome these problems. One such procedure ... epitopes defined by antibodies reactivewith antigens of known primary structure. Unlike most other methods of simultaneouspeptide synthesis, this technique allows the concurrent synthesis of hundreds...
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- glycoprotein methods protocols - biotechnology
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