manipulation of the amino acid composition of prolamins
Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
Ngày tải lên :
22/02/2014, 07:20
... 74% of the cases; residue 186 an Asn in 87% of the cases; and residue 334 a His in 80% of the cases [3] In addition, residue 54 is a Pro in 89% of the cases The importance of the identity of the ... by the amino- acid composition of the shutter region Surprisingly, we found that substitution of Lys335 to Ala affected the rate of latency transition differently in the absence and presence of ... affecting the rate of passage of the RCL through the gate region Based on the aminoacid sequence of the RCL and b strand 5A being directly continuous, it may be proposed that movements of the RCL during...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
Ngày tải lên :
22/02/2014, 07:20
... calculated for the apoenzyme in the presence and absence of metals (Table 3) The nature of the metal present clearly affected the Ea of BLXI inactivation It was remarkably high in the presence of Co2þ ... both the Ea of inactivation and the temperature at which the enzyme starts inactivating at a measurable rate increase from the apoenzyme to the Mn2þ-containing enzyme, loss of the metal cofactor ... precipitation temperatures The lower stability and lower Ea of inactivation of BLXI in the presence of Mg2þ reflect the fact that the enzyme binds Mg2þ with lower specificity (the Kact of Mg2þ is 10 times...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
Ngày tải lên :
18/02/2014, 17:20
... interaction between the flavin, Y308 and the nicotinamide of NADP+ is precisely tuned by selecting amino acids that face the other side of the tyrosine phenol ring The specific volumes of the above-mentioned ... shown) Therefore, the amino acid changes introduced not prevent assembly of the prosthetic group and not impede the production of a folded protein, although, as mentioned above, they may affect the ... the aim of obtaining a better understanding of the structural and functional role of this tyrosine and the interacting amino acids C266, G267 and L268 The data support the hypothesis that the aromatic...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
Ngày tải lên :
18/02/2014, 18:20
... into amino acids is tightly correlated with the expression of the genes involved [15] Thus, we analysed the diurnal expression of the genes encoding the enzymes of nitrogen assimilation in the ... 28.0 1.8 (24 h) The standard errors for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents for glutamine (C), glutamic acid (D), asparagine ... 1.58 (light) and 21.90 1.93 (dark) The standard errors for the individual amino acid contents are of the same order of magnitude as those of the total amino acid contents Phloem exudates and xylem...
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Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot
Ngày tải lên :
23/03/2014, 04:20
... about the effects of CMR on NF-jB activity in macrophages The composition of the diet is known to be important in the development of atherosclerosis [24–26], and a major dietary determinant is the ... expressed as a percentage of the total radioactivity in the cells at the end of the loading period (time 0) and are the mean of three separate experiments Error bars show the SEM The efflux curves were ... fish oil, respectively) they also contained a complex mixture of fatty acids which reflects the composition of the parent oils and of physiological CMR derived from them [28] The triacylglycerol ⁄...
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Báo cáo khoa học: "Bioavailability of the amino acid-attached prodrug as a new anti-HIV agent in rats" pdf
Ngày tải lên :
07/08/2014, 23:22
... demrofrep LA1050-PV rof alumrof eninala dica onima eht yb ytilibaliavaoib fo tnemevorpmi ehT etadidnac gurdorp wen a rof yrotcafsitas erew amsalp ni noitartnecnoc fo ecnanetniam eht dna ,mrof ... noitartsinimda laro eht roF esod eht si D dna noitartnecnoc amsalp eht si C erehw ,t · lek-pxe · dV/D = )t(C saw noitcejni suonevartni rof amsalp ni noitartnecnoc eht rof alumrof ehT noitaterpretni ... fo esod a ta noitcejni suonevartni gniwollof amsalp eht ni dnuof ylidaer saw ti ,revewoH gk/gm 001 fo etar esod a ta star ot noitartsinimda laro gniwollof amsalp eht ni detceted ton saw 2050-PV...
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Tài liệu THE ROLES OF AMINO ACID CHELATES IN ANIMAL NUTRITION pdf
Ngày tải lên :
15/02/2014, 16:20
... to indicate the net utilization of minerals Absorption of mineral ions is dependent upon numerous factors, including the levels of the elements ingested, the age of the animal, Ph of the intestinal ... covalent bonds The average weight of the hydrolyzed amino acids must be approximately 150 and the resulting molecular weight of the chelate must not exceed 800." (0 ) This book is about amino acid chelates ... EVALUATION OF THE NUTRITIONAL EFFICIENCY OF AMINO ACID CHELATES 86 Silvano Maletto and Germano Cagliero AN ASSESSMENT OF LONG TERM FEEDING OF AMINO ACID CHELATES 106 Robert B Jeppsen SECTION CATTLE THE...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
Ngày tải lên :
18/02/2014, 13:20
... lack of domain B protruding out of the TIM barrel in the place of loop connecting the b3-strand to the a3-helix [9,21] Sharing domain B by rBATs and GH13 enzymes, and especially the sequence of the ... possible evolutionary events leading to separation of the heavy-chain subunit of these amino acid transporters from the enzymes involved in the metabolism of starch and related saccharides Results and ... is the light subunit that possesses the amino acid transportation activity, although without interacting with the heavy subunit it is unable to reach the plasma membrane Thus, the role of the...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
Ngày tải lên :
19/02/2014, 12:20
... 4¢-phosphopantothenic acid, CTP and cysteine The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate) ... synthetase CoaB (Eur J Biochem 271) 169 Fig Analysis of the enzymatic activity of His-CoaB R206Q and A276V The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of ... and that the partial blocking of the Dfp (CoaBC) enzyme is overcome in this way The molecular basis for the conditional lethality of the dfp-707 mutant is that one amino acid residue of the N-terminal...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... can therefore postulate that, when the segment beyond W140 was removed, the secondary structure of the protein disintegrated Using the peak at 222 nm of the wild-type protein as an index of helical ... CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein However, in comparison with the wild-type protein, the mutant W140A retains significant ... that the apparent association constant of SNase(1–126) bound to pdTp in the presence of Ca2+ is approximately threefold lower than that of the wild-type protein Therefore, some or all of the residues...
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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx
Ngày tải lên :
20/02/2014, 23:20
... with the nt sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table Summary of the purification of DNases I from the pancreases of three species of snake ... not The same was true for these mutants of the rat and mouse DNase I (Fig 3) These findings demonstrate that the nature of the amino acid at position 130 may generally and markedly affect the thermal ... cloned the cDNA of each A single amino acid (aa) substitution was confirmed to affect the thermal stabilities of vertebrate DNases I and, furthermore, one of the postulated mechanisms whereby thermal...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
Ngày tải lên :
07/03/2014, 03:20
... (indicated in bold) The enrichment for a given GO category was computed as the ratio of the distribution of the amino acid- regulated genes* versus the distribution of the genes spotted onto the microarray** ... contribute to these multistep processes, including the metabolic conditions of the cell, nature of the stimulus, RNA binding factors and the sequence of the target mRNA [35] Another amino acid sensing ... and amino acid starvation had opposite effects on the expression of a large group of genes involved in the synthesis, transport and use of amino acids The experimental procedures may explain the...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... transporter to the internal membrane side is the rate-limiting step and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outside of the membrane ... interpreted as the first evidence for a restricted velocity in the translocation step of the loaded transporter by the sterical conformation of the substrate The introduction of polar side groups in the ... orientation of the substrate within the substrate binding site of PAT2, and this may simultaneously avoid the interference of the polar side chain with corresponding amino acid residues in the binding...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
Ngày tải lên :
07/03/2014, 21:20
... with the presence of an a-helix [13,14] In contrast, the E129G mutant exhibited less CD than the others Thus, while most of the mutations of negatively charged glutamic acid or aspartic acid ... 33% lower than that of the wild-type protein The thermodynamic parameters of the glycine sustitution mutants also differed greatly from the wild-type protein The Tm values of these mutants ranged ... key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles in generating these...
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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
Ngày tải lên :
08/03/2014, 08:20
... three-dimensional structures of a -amino acids and those of b -amino acids and posed the following questions Which structures of b -amino acids overlap the canonical structures of a -amino acids? Subsequently, ... structures of the b -amino acids that best fit are shown in Fig They all correspond to gauche(+) values of the h angle The methyl group of b2-HAla occupies a position close to that of CH2b of Pro or the ... representation of a-, b2- and b3 -amino acids with the torsion angles /, h and w For the b2 and b3 -amino acid substituted-SP analogues, the methyl side chain of b-HAla exhibits the same orientation as the...
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx
Ngày tải lên :
16/03/2014, 01:20
... [15] On the other hand, the hydrolysis rate varies with the substituent on the amino moiety of the substrate, confirming that the formation of acyl enzyme (AE) is the rate-limiting step of the reaction ... protonated amino group in the proximity of the oxyanion can be found in the rest of the Ntn hydrolases [3] From another point of view, the hydrogen bond between the oxyanion and the aNH3+ of SerB1 ... in the conformation of the enzyme Substrate binding causes considerable shortening of the hydrogen bonds of the side chain hydroxyl of SerB1 with the backbone amino group of GlnB23 and with the...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
Ngày tải lên :
16/03/2014, 10:20
... energies of the original resiDG i;j due i in the native protein with the rest of the residues, self DDG k mutị are the changes of the self energies of the residues k that change either their ionization ... bonds with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ... Substitution of these residues by the hydrophobic side-chain of methionine decreases in these two enzymes both the Km for NMP and the kcat compared with the parent molecules [16,18] In conclusion, the amino...
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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redefinition of the I-conotoxin superfamily pot
Ngày tải lên :
16/03/2014, 22:20
... for modification, i.e either on the second amino acid from the N-terminus, or on the third amino acid from the C-terminus However, there has not been an opportunity to further define in what sequence ... further molecular definition of the I-superfamily In contrast with all other conotoxin gene superfamilies, we found that the Table Amino- acid sequences of I-conotoxins with a D -amino acid near the ... amino acids at the C-terminus of r11a are not conserved, including the amino acid to be modified (Phe in the case of r11a compared with Leu in the case of r11c) Furthermore, in the mature toxin...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
Ngày tải lên :
17/03/2014, 03:20
... the importance of these amino acids in the interaction between the 4-kDa peptide and the 43-kDa protein The secondary structures of these two fragments, as revealed from NMR spectroscopy of the ... variants of amino acids, shown in red, had a >100-fold decrease in affinity (D) Summary of alanine scanning of the 4-kDa peptide The results are expressed as the ratio of dissociation of the variant ... binding to the 43-kDa protein The topology of the sidechains of Ile25, Val29, Phe31 and Ile33 in the 4-kDa peptide was similar to that of the active area in insulin (Fig 7F) If the mechanism of the...
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Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Ngày tải lên :
17/03/2014, 17:20
... change [10] The higher stabilization of the semiquinone form observed for the Y238S mutant in the free form may be the result of a better interaction of R285 with the N(1)-C(2)ẳO locus of the reduced ... dismutation of EFlseq to the oxidized and reduced forms, with the endpoint containing the thermodynamically stabilized amount of semiquinone The Y238S mutant showed a higher percentage of the thermodynamically ... equilibration method of Minnaert [17], in order to assess changes in the thermodynamic properties of the avin centre caused by the mutation and to explain the different thermodynamic stability of the semiquinone...
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