manipulation of lytic enzyme systems involved in biocontrol agents of plant pathogens
Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... previously shown that proteinprotein interactions can result in information transfer, imprinting effects and can modify the regulatory properties of the enzymes involved in this complex [1620] GAPDH ... the binding of CP12 The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes observed for the binding of the substrate when the wild-type recombinant ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248254 Sali, A & Blundell, T.L (1993) Comparative protein modelling by satisfaction of spatial restraints J Mol...
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7Microbial Enzymes in the Biocontrol of Plant Pathogens and aEnzymes in the Environment: Activity, Ecology and Applications - Chapter 7PestsLeonid Chernin and Ilan ChetThe ppt
... inhibited in all chitin-containing fungi The degree of inhibition was proportional to the level of chitin in the cell wall of the target fungus Combining the two enzymes resulted in a synergistic increase ... incognita and exposed its chitin layer (280) VII MOLECULAR APPROACHES FOR THE IMPROVEMENT AND CREATION OF NEW BIOCONTROL AGENTS A Manipulation of Lytic Enzyme Systems Involved in Biocontrol Agents ... plant- growth-promoting strain with biocontrol potential against various pathogens (195) C Genetic Systems for Regulating the Production of Enzymes and Secondary Metabolites Involved in Biocontrol Activity of...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... presence of DNA-binding proteins involved in regulation of the gene expression of indole-3pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum lipoferum FS Biosci ... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ThDP binding sites are located in narrow clefts at the interfaces...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... differences cosegregated in 22 independent meiotic recombinants in a cross involving strain IJ2 (sta2-29::ARG7 sta3-1) and an interfertile ecotype of C reinhardtii known as C smithii (strain CS9) This latter ... typical red iodine stain Stains of others strains result from various levels of phenotypic complementation indicating, in most cases (except for strain N), a partial restoration of amylose biosynthesis ... quantity of Chlamydomonas starch involved in the assay) in the complemented strains varied from 44 to 84% when compared with that of the wild-type strain It is clear that six strains (out of three...
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Báo cáo khoa học: The 3-ureidopropionase of Caenorhabditis elegans, an enzyme involved in pyrimidine degradation pptx
... product of uracil, 3-aminopropionate (b-alanine), for example, can be condensed with histidine to form the dipeptide carnosine Carnosine and other similar dipeptides containing nonproteinogenic amino ... taking a monomer mass of 43.2 kDa into account, points to a dodecamer (Fig 4) To support this nding, the oligomeric state of the enzyme, the recombinant protein was subjected to gel ltration using ... using 4Â,6Â-diamidino-2phenylindole- and Hoechst staining indicated that 3-ureidopropionase is not localized in the nuclei of muscle cells (data not shown) In animals, catabolic processes are often...
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Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... the quantitation of microgram quantities of protein, utilizing the principle of protein-dye binding Anal Biochem 72, 248254 Segel IH (1993) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium ... structure of human m-NAD-ME in complex with fumarate has been determined, the binding network of fumarate in the enzyme has become clear In the fumarate-binding pocket, two arginyl residues, Arg67 and ... E59 mutant enzymes was only one-third to one-quarter of that of the wild-type, suggesting that mutation of Glu59 in the fumarate-binding site causes the enzyme to become less efcient in catalysis...
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Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt
... to be involved in an angiotensin II-induced vascular hypertrophy [15], and in inducing the expression of monocyte chemoattractant protein-1 [16], which plays a key role in the development of atherosclerosis ... Essential role of ATF-1 in induction of NOX1, a catalytic subunit of NADPH oxidase: involvement of mitochondrial respiratory chain Biochem J 386, 255–261 Fan C, Katsuyama M, Nishinaka T & Yabe-Nishimura ... predominant role of the ATF-1-myocyte enhancer factor 2B (MEF2B) cascade in superoxide production in vascular smooth muscle cells by inducing the expression of the NOX1 gene Results Determination of...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray results ... ANK3 Gene title cofactor of BRCA1 integrin, beta staufen, RNA binding protein, homolog (Drosophila) high mobility group nucleosomal binding domain ankyrin 3, node of Ranvier (ankyrin G) Signal Log...
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Risks Involved in the Use of Herbal Products
... inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen radical-forming enzymes, ... this kind of information are found in Ody (1993) and Moore (1995) 14.2 Inadequate Testing of Herbal Medicine Products Many medicinal herbs have not yet been subjected to testing in human clinical ... may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify the side effects of...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... suggesting that the heterologous expression of genes encoding metalloproteins can lead to incorrect metal ion incorporation This is observed in rubredoxin and desulforedoxins where zinc, instead of ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature ... not be considered in the light of the results obtained in the present study Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C ammoniagenes R2F is envisaged...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved in the induction of dimerization [43] Each of these...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment revealed an ORF showing amino acid ... Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a manner similar ... transcripts during development and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression during...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... consistently pointed to the formation of glutamate, proving unambiguously that the complex did indeed hydrolyze glutamine efficiently Kinetics of the glutaminase reaction The kinetics of glutamine hydrolysis ... Glutamine was effective in protecting the enzyme from inactivation and its effect was again dose-dependent, suggesting that inhibition by DON occurs at the active site or the glutamine-binding...
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Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt
... isoalloxazine ring (Fig 5B, broken line, number 3) Phe in place of Trp66 resulted in a protein with noncovalently bound FAD, again showing no enzyme activity, and isolation of the flavin cofactor ... domain of MABO to other proteins of the sarcosine dehydrogenase and oxidase family may indicate that this is the site of attachment of tetrahydrofolate to the enzyme c-Aminobutyrate produced during ... positioning of the flavin cofactor at the active site was not affected Replacement of tryptophan with serine (W66S), also abolished covalent binding of FAD and resulted in an inactive enzyme variant...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... Results The enzyme involved in catalysis of the fifth step of citrulline biosynthesis in wild watermelon leaves During citrulline biosynthesis, N-acetylornithine is converted into ornithine by AOD ... [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic enzymes is scarce, ... conditions owing to its high thermostability and insensitivity to the inhibition induced by citrulline and arginine However, many different enzymes are involved in the metabolism of citrulline (Fig...
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Tài liệu Báo cáo khoa học: Separation of a cholesterol-enriched microdomain involved in T-cell signal transduction doc
... by modifying h-toxin (perfringolysin O), a cholesterol-binding, pore-forming cytolysin produced by Clostridium perfringens [18] Ch and BCh are noncytolytic derivatives of h-toxin that bind specifically ... abolishes their binding to plasma membranes [17–19] This is in remarkable contrast to cell binding by filipin, another cholesterol-binding reagent Filipin staining is significantly retained under the ... PS distributes in the inner leaflet of plasma membranes in mammalian cells Although there is insufficient information about the inner leaflet of lipid microdomains, the inner leaflet of BCh-bound membrane...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... transcription terminator The resulting fragment only contains the initial 67 bp and the final 68 bp of the pur4 coding sequence It was inserted in plasmid pGM9 The resulting construct was introduced, ... point, the procedure described in Hopwood et al [14] was followed DNA sequencing was made by the dideoxy-chain termination method [25] using the Amplitaq dye-terminator sequencing system (Perkin...
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Báo cáo khoa học: Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation ppt
... expressing [3H]cGMP-binding activity Identification of a PDE species expressing [3H]cGMPbinding activity is the first step in the exploration of the role of cGMP binding to the noncatalytic site in ... conformational change is indeed involved in PDE regulation Suppression of cGMP binding during activation of Pabcc Two possible stages for cGMP binding to Pabc are expected in PDE regulation: during Pabcc activation ... [3H]cGMP-binding activity and that Pc inhibits [3H]cGMP binding to Pabc We also show that the cGMP binding to Pabc is suppressed during Pabcc activation, i.e cGMP binding is not involved in Pabcc...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... obtained indicate that, although the substrate-binding site is located in the C-terminal domain, the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in ... domain Taking into account the important role of Ptas, the results obtained in the present work, dissecting the different domains forming E coli Pta, will help in the future manipulation of these ... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig 2) PCR reactions performed in...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... is directly involved in the regulation of amino acid and protein metabolism, as many of the genes involved in these processes are not regulated in GCN2) ⁄ ) cells These results are in good agreement ... in Table S1 in the regulation of gene expression in response to amino acid starvation However, comparison of the regulatory mechanisms involved in the control of gene expression by amino acid availability ... amino acid limitation C Deval et al Table Enrichment of the amino acid-regulated genes according to the biological process in which they are involved Enrichment was determined using FATIGO software...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25