mammals parasitic protozoa and yeast
Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf
... conservation [12,13], and are thought to share a similar functional mechanism Thus, DnaK and mtHsp70 cooperate with the co-chaperones DnaJ and GrpE in bacteria, and with Mdj1p and Mge1p in yeast mitochondria, ... 30 °C, protein and ATP concentrations were lM and mM, respectively NRLLLTG (NR) peptide was added at 0.5 mM DnaJ and GrpE concentrations were 0.5 lM and 1.5 lM, respectively Mdj1p and Mge1p were ... times; (b) the 33 and 31 kDa species were found only in small proportions; (c) the 46-kDa band was predominant at short and intermediate times, and (d) after 40 min, the 44 kDa band was the most...
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Báo cáo khoa học: A mitochondrial cytochrome b mutation causing severe respiratory chain enzyme deficiency in humans and yeast doc
... yeast) and the 14 kDa subunit of the bc1 complex (green) FEBS Journal 272 (2005) 3583–3592 ª 2005 FEBS All muscle biopsies and experimental procedures were undertaken with the understanding and ... effect and distorted the architecture of the Qo site FEBS Journal 272 (2005) 3583–3592 ª 2005 FEBS E L Blakely et al Novel cytochrome b mutation in human and yeast A B Fig Western blotting and Blue ... of naturally occurring yeast mutants and revertants [29] that have a human counterpart [15], and by introducing the equivalent human disease mutation into the homologous yeast enzyme Consequently,...
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Báo cáo sinh học: "Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression" ppt
... dividing and then proliferate and get progressively smaller [22] Why yeasts and Schwann cells coordinate cell growth and cell-cycle progression so differently? The lifestyles of yeasts and animal ... T, Dumont JE, Maenhaut C: IGF-1 or insulin, and the TSH cyclic AMP cascade separately control dog and human thyroid cell growth and DNA synthesis, and complement each other in inducing mitogenesis ... hypothetical model showing why the progeny of large and small daughter cells eventually return to the mean population size over time if large and small cells grow and progress through the cell cycle at the...
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Feasibility of probiotic lactobacillus and yeast as oral vaccine carrier against coronaviruses
... understanding, support and concern throughout this period My family especially my husband for their patience and encouragement throughout these years Many things have happened and your love and ... Cytokines and biological activities 35 Table 2.1 Bacterial and yeast strains used in adhesion study 51 Table 2.2 Bacteria and yeast strains used in the study 56 Table 2.3 Molecular vectors and related ... (IL-2 and IFN-γ) and Th2 (IL-4 and IL-5) cytokine production by Peyer’s patches cells from mice immunized with PP/SARS-S-RBD and P.pastoris 181 Figure 5.13 Kinetics of Th1 (IL-2 and IFN-γ) and...
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Effects of sugar concentration and yeast inoculation strategy on mango wine fermentation
... individual yeasts – its compatibility with other yeasts and the effects on growth rate and biomass development Suppression of one yeast by the other can result in its reduced metabolic activity and ... esters and higher alcohols and more acetic acid and acetaldehyde were produced in high sugar fermentation The effects of a non-Saccharomyces yeast, Williopsis saturnus var mrakii NCYC 500 and a ... leucine and isoleucine, with a protein content of approximately 0.8 g/100 g (Tandon and Kalra 1984) Lipids constitute less than 1% (w/w), with triglyceride being the major component (Gholap and Bandyopadhyay...
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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... baker’s yeast and wine yeast In Enzymes, Biomass, Food and Feed (Reed G & Nagodawithana TW eds), pp 322–351 VCH, Weinheim 12 Reed G & Nagodawithana TW (1991) Baker’s yeast production In Yeast Technology ... Netherlands), pmol of each primer (Isogen, De Meern, The Netherlands or Biolegio, Nijmegen, The Netherlands) and lL of cDNA template (equivalent to ng of RNA) Amplification, data acquisition, and ... proteins, PFK2, TDH3, GPM1 and -3, ENO2, PDC5 and ADH6 showed interaction with the yeast E3 ubiquitin ligase RSP5 in a binding assay using protein chips [44] For PFK, GPM, PDC and ADH this is in agreement...
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Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
... Materials and methods Yeast strains, plasmids and growth conditions All yeast strains used in this study are in the YPH499 background and are listed in Table Yeast strains were grown in 1% yeast ... Ste20p and Cla4p may inhibit sterol biosynthesis Notably, we observed (using TLC and GLC ⁄ MS) higher amounts of sterols in the ste20D and the cla4D strains TLC separates free sterols and SE, and ... Many aspects of sterol homeostasis are conserved between yeasts and humans; and ergosterol, the predominant sterol of yeast, is structurally and functionally related to sterols of higher eukaryotes...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... of Vrp1p and Las17p in yeast and WIP and WASP-family proteins in mammalian cells are direct and result in constitutive and apparently stable complexes [28,29,31,32] In yeast, Vrp1p and Las17p ... interaction with actin and that GSTC-Vrp1p465)533 binds endogenous actin in yeast lysates and directly binds purified yeast and rabbit skeletal muscle G-actin in vitro (Fig 4B–D and data not shown) ... Saccharomyces cerevisiae microtubule and microfilament cytoskeleton Yeast 13, 399–434 Engqvist-Goldstein AE & Drubin DG (2003) Actin assembly and endocytosis: from yeast to mammals Annu Rev Cell Dev Biol...
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Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf
... between Rio1p and Cka1p or Rio1p and Cka2p, respectively, in yeast strains disrupted for either CKA1 or CKA2 Genotypes are indicated below (C), and alleles in brackets denote the tagged (and immunoprecipitated) ... related to yeast Rio1p and have both a CK2- and a K-domain, although the direct sequence similarity is low We have cloned both types of human cDNAs, ad 034 and SUDD, as myc3tagged version and expressed ... cycle and highly resistant to proteolytic degradation [40] Miscellaneous procedures Yeast were grown on standard media [41] and transformed as described by Gietz et al [42] Microscopy and cell...
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Tài liệu Báo cáo khoa học: L-Arabinose transport and catabolism in yeast doc
... from d-xylose by both yeasts and from l-arabinose in the case of C arabinofermentans [25] The co-utilization of d-xylose and l-arabinose in mixtures by Candida entomaea and P guilliermondii had ... transport and catabolism in yeast tinct transport systems operating simultaneously and differing in substrate affinity (half-saturation constants of 125 mm and 0.1 mm for low-affinity and highaffinity ... xylose transport in Candida intermedia PYCC 4715 FEMS Yeast Res 3, 45–52 11 Nobre A, Lucas C & Leao C (1999) Transport and util˜ ization of hexoses and pentoses in the halotolerant yeast Debaryomyces...
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Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx
... media and genetic procedures ALR2-HIS-r Yeast strains were grown at 28 °C in YPD medium (yeast extract ⁄ peptone ⁄ glucose), standard SD medium (0.67% yeast nitrogen base, 2% glucose, and amino ... Alr1p and Alr2p in the split-ubiquitin system Alr1p and Alr2p were analyzed using the split-ubiquitin system Cells expressing NubG and Cub fusions of Alr1p and Alr2p were mated cross-wise and diploids ... transformed with YCpALR1-myc and YEp351-HA (lane 1), YCpALR1-myc and YEpALR2-HA (lane 2), and YCp211-myc and YEpALR2-HA (lane 3) Total cell extracts were prepared, and equal amounts of protein...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... membranes from a yeast mutant lacking mitochondrial DNA and a yeast mutant with a truncated cytochrome b gene The wild-type (WT), q°, and CKL57 yeast strains were grown on YPD medium and mitochondrial ... constructed as follows The haploid strains VZ1 (D7) and MES8 (D6), VZ2 (D7) and LLD9 (D8), JDP1 (D9) and LLD9 (D8), JDP2 (D9) and UBL2 (D10), were mated and the resulting diploids were sporulated to ... VZ4, VZ6, VZ14 and VZ9 were then selected for Trp+ and Leu+, Trp+ and His+, His+ and Ura+, or His+ and Leu+ prototrophy, respectively Other yeast genetic methods used were as described in [23]...
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Tài liệu Báo cáo : Integrated Cultivation Technique for Microbial Lipid Production by Photosynthetic Microalgae and Locally Oleaginous Yeast pptx
... KKU-S2 and oleaginous yeast T maleeae Y30 in an integrated technique to improve the biomass and lipid yield using CO2 emissions from yeast fermentation resulted to reduce cultivation costs and also ... CaCl2 0.0025, MnCl2 0.0005, CuSO4 0.0003 and yeast extract 0.75 and pH was adjusted to 5.5 before sterilization C Effect of Nitrogen Concentration on Growth and Lipid Production Batch cultivations ... of air and CO2 from yeast fermentation was aerated during the cultivation A schematic of a yeast fermentation flask connected to microalgae flask is shown in Fig The CO2 produced by the yeast...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... the cytosolic and membrane-bound forms of yeast PtdEtn-PLD and examined the regulation of PtdEtn-PLD activity under certain growth, nutritional and stress conditions MATERIALS AND METHODS Chemicals ... Whatman Yeast Nitrogen Base (YNB) lacking amino acids and ammonium sulfate were from Difco Dioleoyl-PtdEtn, PtdInsP2 and all other reagents were from Sigma Yeast strains The wild-type yeast strain ... (I+) and/ or mM choline (C+) Other amino acidrich media included: YPD [yeast extract and Bactopeptone (YP) containing 2% dextrose]; YPA (YP containing 0.05% glucose and 2% potassium acetate); and...
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Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling doc
... between yeast and mammalian cells This has led to the construction of ingenious systems that provide for the mutual exchange of signals between heterologous GPCRs and yeast G-proteins in yeast ... classes, and a number of GPCRs of human and other species are able to interact with Gpa1p and activate pheromone signaling in yeast [73–75] Many other human GPCRs can also function as yeast signaling ... interact with the yeast Gbc complex, but is not able to interact with the yeast Ste2p receptor, and it was fused to the truncated Ste2p receptor Although a gpa1D yeast strain harboring the yeast rat...
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... [23], and in yeast, Lsm2p and Lsm7p fail to localize to P-bodies in cells deleted for LSM1 [24] In yeast, Lsm8p fails to accumulate in the nucleus when cells are depleted of Lsm2p or Lsm4p [13], and ... Knuckle I and II MPS26 was transformed with plasmids (A) pMR70, pMR80, pMR84 and pMR104; (B) pMR117, pMR126, pMR140, pMR141, pMR115 and pMR124; (C) pMR114, pMR116, pMR123 and pMR125; and (D) pMPS8, ... or below and only when expressed without a tag from the strong ADH1 promoter Thus, human LSm1 and B Fig Correlation between viability and correct localization of Lsm1 and Lsm8 hybrid and mutant...
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Báo cáo khoa học: Inactivating pentapeptide insertions in the fission yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine finger motif docx
... b-strand b4 in the five-strand parallel b sheet that comprises the core of domain I (Figs 2A and 3A) The b4 strand is the central strand in the sheet [9]; it is likely that disruption of this strand ... K12), between a1 and a2 (K32), between a2 and b1 (K22, K23 and K3), between b2 and a4 (K11) and at the N-terminal end of b3 (F46, F49) Two domain I deletion constructs (K33DK27 and K33D22) were ... (lysines 44 and 45 in fission yeast Rfc2) and the K33D22 deletion removes these altogether, replacing the sequence LKKT with GVP (see Fig 2A and Table 1) The function of a2 helix and the conserved...
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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... Mg(CH3COO)2, 100 mm KCl and mm dithiothreitol, and suspended in the same buffer containing mm PMSF and 4000 U RNasine The cell suspension was mixed with glass beads and the yeast cells lysed as ... in the yeast cells Furthermore, peptide mapping suggested that both phosphorylation by the endogenous and the mammalian kinases occurred at the same site in yeast eEF2 [23] The endogenous yeast ... FEBS G Bartish et al Role of Thr56 and Thr58 for eEF2 function in yeast Table Strains and plasmids used in the present study Euroscarf (Frankfurt, Germany) Strains and plasmids Source YOR133w (Mat...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... site [26], and that it has opposing allosteric effects on PP2Ac and PP6c [27] Rapamycin pathway-independent association of TIPRL, PP2Ac and a4 in human K562 cells Although in yeast Tap42 and type ... was performed with the complete PP2Aca and PP2Acb cDNAs, with the longest PP4c and PP6c cDNAs, encompassing residues 175–307 and 106–305, respectively, and the shortest cDNA, corresponding to ... serum-free medium and the nuclear (N) and cytoplasmic (C) fractions were separated and probed with specific antibodies 7.5 lg of total protein extract were loaded on each lane Both TIPRL and PP2Ac are...
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Báo cáo khoa học: Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx
... the product peptide and substrate peaks Purification of yeast aspartyl aminopeptidase Yeast cells were grown in YPD medium (10 gÆL)1 yeast extract, 20 gÆL)1 bactopeptone, and 20 gÆL)1 glucose) ... mass of native yeast aspartyl aminopeptidase The molecular mass of the native yeast aspartyl aminopeptidase was determined by FPLC using a Superose column The purified enzyme and a standard marker ... activity in Mono Q fractions I and II using a Superose column (s, d) Activity of fractions I and II, respectively (B, C) Results of SDS ⁄ PAGE analysis of fractions I and II Lane numbers correspond...
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