magnetoresistance of two dimensional and three dimensional electron gas in laalo3 srtio3 interfaces

Host viral interactions host factors in coronavirus replication and coronaviral strategies of immune evasion

Ngày tải lên : 09/09/2015, 10:07

... Three main protease domains are involved in this auto-proteolytic processing of the replicase Two papain-like proteases (PLpro), PLP1 and PLP2 of nsp3 are primarily involved in the cleavage of ... regulatory domains such as the 5’- and 3’ UTR, and TRS of leader (TRS-L) and each ORF (TRS-B), and this is mediated by protein-RNA and protein-protein interactions comprising of both viral and cellular ... regulatory protein 1, is a RNA binding protein that coordinates the expression of ferritin and transferrin receptor during iron metabolism by binding to iron-responsive elements in the 5’ and 3’ UTRs...

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Báo cáo y học: " Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" docx

Ngày tải lên : 13/08/2014, 09:20

... proteins of clinical interest, including cytokines [54,55] and viral envelope proteins [56-59] Not only does Fc-fusion provide a very convenient way to express and purify proteins of interest (using ... gp120-DC-SIGN interactions 2G12 inhibition of gp120 binding to DC-SIGN was revealed in several distinct assays: inhibition of both Fc-gp120 and whole virus binding to two different DC-SIGN-expressing cell ... ligands So far, information suggests that cyanovirin inhibits 2G12 binding to Env [3,60], but 2G12 does not inhibit cyanovirin binding [3,7]; DCSIGN-Env binding does not inhibit 2G12 binding [60],...

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Báo cáo y học: " Correction: Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" doc

Ngày tải lên : 13/08/2014, 09:20

... formats Results are expressed as % of residual infection, with 100% representing infection in the absence of mAb Results are representative of two experiments Page of (page number not for citation ... neutralizes HIV-1 JR-CSF effectively in a postCD4 assay format The neutralization activity of mAbs A) b12, B) X5, and C) 2G12 was assessed in the standard (closed circles) and post-CD4 (open circles) ... (open in the of mAbs b12, assay and and 2G12 was assessedactivity standard postCD4 B) X5,format The neutralizationcircles) neutralization Figure 2G12 neutralizes HIV-1 JR-CSF effectively in aA)...

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Tài liệu Báo cáo Y học: A pool of Y2 neuropeptide Y receptors activated by modiﬁers of membrane sulfhydryl or cholesterol balance pot

Ngày tải lên : 22/02/2014, 04:20

... expressed adhesion and interaction factors such as integrins (e.g a1-integrin, a laminin and collagen receptor), cadherins, and glypicans Adhesion system ligands possessing multiple vicinal dithiols ... systems Intramembrane targets of PAO could also include G-protein b- and c-subunits that contain vicinal cysteines, as well as some rab and ras G-proteins The stimulation of Y2 binding by ﬁlipin should ... the a-actinin component) indicates a direct involvement of basal lamina molecules such as ﬁbronectin, collagen IV, or laminin in cytoskeletal handling of the plasma membrane nicotinic receptor...

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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

Ngày tải lên : 08/03/2014, 19:20

... understanding the functioning of a cell Replication and recombination machineries cooperate to maintain biological inheritance and genomic integrity While replication enables the formation of two ... Institute of Chemistry Slovenia Inroduction DNA replication, the process of copying double-stranded DNA, and DNA recombination, the process of breaking DNA strand and joined to a different strand, ... staining will probably be applied more broadly in the analysis of the DNA distribution of specific tissues and cells of interest Fig EdU incorporation and staining visualizes DNA replication in...

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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx

Ngày tải lên : 18/06/2014, 15:20

... expression of genes encoding cytokines and chemokines and anti HBsAg serum titers in vaccinated healthy donors Correlation Correlation between expression of genes encoding cytokines and chemokines and ... the gold standard for virtually all vaccines of current use for the prevention of infectious diseases In contrast, monitoring of cellular immune responses following natural or vaccine induced immunization ... reverse transcribed and amplified in the presence of primers and probes specific for β-actin house keeping gene and genes encoding different cytokines Expression of IFN-γ and IL-2 genes was significantly...

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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx

Ngày tải lên : 18/06/2014, 18:20

... family of proteins is involved in the development of liver diseases [9,10] The Bcl-2 family of proteins is defined as the key regulator of apoptosis in the mitochondria-dependent way They consist of ... found in HBV infected liver and shown to induce apoptosis through a hitherto unknown mechanism [16,17] It was also indicated to contain a BH3 domain in the N-terminal and naturally expressed during ... Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains revealed the three genotypes share the same conserved amino acid 21–30 BH3 domain is characterized...

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Báo cáo hóa học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" docx

Ngày tải lên : 20/06/2014, 01:20

... family of proteins is involved in the development of liver diseases [9,10] The Bcl-2 family of proteins is defined as the key regulator of apoptosis in the mitochondria-dependent way They consist of ... found in HBV infected liver and shown to induce apoptosis through a hitherto unknown mechanism [16,17] It was also indicated to contain a BH3 domain in the N-terminal and naturally expressed during ... Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains revealed the three genotypes share the same conserved amino acid 21–30 BH3 domain is characterized...

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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

Ngày tải lên : 16/03/2014, 02:20

... a series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and leading to the abrogation of insulin inhibition of gluconeogenesis and glucose ... prevents insulin-induced inhibition of hepatic glucose output, indicating that, in such conditions, cells become insulin insensitive; this is in agreement with studies in which the overexpression of ... and control vs insulin and TNFa plus insulin vs insulin alone (C); **P < 0.01 when compared with insulin alone (B) and TNFa incubation when compared with control (C); ***P < 0.05, TNFa plus insulin...

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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Ngày tải lên : 16/03/2014, 12:20

... properties of a variety of proteins with common biochemical activities, such as the binding to single-stranded nucleic acids and the remodelling of their secondary structures A model involving CNBP ... embryos were incubated during 15 with different kinase inhibitors at 30 °C and then used to analyse in vitro recombinant CNBP phosphorylation in kinase buffer containing 100 lm ATP and lCi [32P]ATP[cP] ... nature of the analysed probe, band-shift intensity increased along with the protein increasing amount Nevertheless, no signiﬁcant differences in binding afﬁnities were observed as a consequence of...

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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

Ngày tải lên : 16/03/2014, 22:20

... the brain, but not from any other tissues examined including the liver, ovary, skin, intestine, heart, muscle and testis crabp1b mRNA was detected by RT-PCR in the skin, intestine, brain and muscle ... the boarder of hindbrain and spinal cord showing transversal distribution of the crabp1b mRNA in r7, neurons of spinal cord (N Sp), dorsal and ventral spinal cord (D Sp and V Sp) and the anterior ... analysis of cellular retinoid-binding proteins The bootstrap neighbor-joining phylogenetic tree was constructed using CLUSTALX [23] The zebraﬁsh (Zf) intestinal type fatty acid-binding protein (I-FABP)...

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

Ngày tải lên : 17/03/2014, 17:20

... thrombin-induced increase in InsP3 level at concentrations that also suppressed the thrombin-induced Ca2+ response Together these results indicate that both InsP3-generating (thrombin) and nonInsP3-generating ... spiking [18,27] In the present paper, we describe that regardless of the type of agonist, stimulating (thrombin) or sensitizing (thimerosal) InsP3 receptors or acting primarily independently of InsP3 ... Ca2+-mobilizing potency of a-thrombin and thrombin-receptor-activating peptide on human platelets: concentration and time eﬀects of thrombin-induced Ca2+ signaling Eur J Biochem 249, 547–555 21 Hussain,...

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Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt

Ngày tải lên : 30/03/2014, 08:20

... Intracellular Na+ and gene expression M Haloui et al Table Effect of ouabain, monensin and actinomycin D on survival of HeLa cells Cells were incubated in DMEM with compounds indicated in the left ... were incubated for h in DMEM with or without ouabain and monensin Na+i, K+i and c-Fos content in the absence of ouabain and monensin was taken as 100% The means ± SE from experiments performed in ... ouabain, µM Fig (A) Representative western blot showing the effects of ouabain and monensin on c-Fos content in HeLa cells (B) Dose-dependent effect of ouabain on intracellular Na+, K+ and c-Fos...

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Báo cáo khoa học: Binding areas of urokinase-type plasminogen activator– plasminogen activator inhibitor-1 complex for endocytosis receptors of the low-density lipoprotein receptor family, determined by site-directed mutagenesis doc

Ngày tải lên : 30/03/2014, 10:20

... mutations into both uPA and PAI-1, and studying binding to VLDLR, LRP-1A, and sorLA We initially chose basic residues for mutation on the basis of evidence for receptor binding involving interactions ... measured the effect on receptor binding of substituting a cluster of three arginines (R108, R109, and R110) in the uPA kringle, but found no change in receptor binding after these mutations (Fig ... time implicate the SPD of uPA in the binding The residues that mediate the binding afnity of the A-chain of uPA remain to be established In the long term, exact mapping of the interaction surface...

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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

Ngày tải lên : 19/06/2014, 22:20

... expressed within inflammatory brain lesions in MS [18,39-41], and CCL3 has been demonstrated in cerebrospinal fluid of MS patients with relapsing-remitting disease course [42] In Interestingly and consistent ... restricted areas of the spinal cord displaying focal inflammation and demyelination (Fig 2E) Quantification of CCR1, CCR2 and CCR5 mRNAexpressing cells in relation to the stage of demyelinating activity ... formation of focal areas of demyelination [31] and axonal injury and loss [32] Our results demonstrate a prominent accumulation of monocytes and macrophages expressing CCR1, CCR2 or CCR5 mRNA within and...

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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Ngày tải lên : 19/06/2014, 22:20

... side of injection, immunostained for VCAM-1 Arrows indicate VCAM-1 immunostaining Scale bar is 50 μm (B, D) Quantification of intensity of VCAM-1 staining as described in Material and methods in ... [13,18,49,50] the role of endocannabinoids in MS remains uncertain While most of studies on ECS and MS focused on established disease, understanding the role of endocannabinoids during the induction phase ... immunohistochemistry studies and participated in the interpretation of data and revising manuscript draft FC, FL and FD participated in the design of the study, interpretation of data and revision of manuscript...

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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx

Ngày tải lên : 21/06/2014, 00:20

... 0), SINRo = SINRc = 2πs s†R−1s and we get SINRopt1[K] = SINRconv1[K] = 2K πs s†R−1s (19) Computation and comparison of SINRo and SINRc are done in [31] in the presence of one rectilinear interference ... signal (SINRo

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Báo cáo hóa học: " Research Article Optimal Design of Nonuniform Linear Arrays in Cellular Systems by Out-of-Cell Interference Minimization" ppt

Ngày tải lên : 22/06/2014, 19:20

... 1.26 λ and internal spacing Δ23 3.6 λ It is interesting to compare this result with the case of a line -of- sight channel that is shown in Figure In this latter scenario, the optimal spacings are ... η and the antenna spacings Δ through nonlinear relationships The first problem we tackle is that of finding the set of optimal spacings Δ that minimizes the average (with respect to fading) interference ... design in interferencelimited scenario, the interference power minimization is analytically tractable and highlights the justification for unequal spacings Therefore, the problem of minimizing the interference...

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The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway ppt

Ngày tải lên : 09/08/2014, 10:20

... QKRAA, and 40 nM for QRRAA (Figure 4c) Finally, the involvement of NO in SE-triggered pro-oxidative signaling was investigated As can be seen in Figure 5a, preincubation of M1 cells with the NOS inhibitor ... saline (PBS) at pH 7.5 The efficiency of binding was determined by quantification of the residual concentration of peptides in the buffer after overnight incubation with the beads SE-expressing ... A, alanine; C, cysteine; D, aspartic acid; E, glutamic acid; G, glycine; HLA-DRB1, human leukocyte antigen-DRB1; I, isoleucine; K, lysine; L, leucine; Q, glutamine; R, arginine; T, threonine; V,...

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