lytic enzymes involved in the biocontrol activity of other fungi
Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... [23] In the present study we have cloned the murine genes coding for the enzymes involved in the salvage pathway of GDP-L-fucose L-fucokinase and GDP-L-fucose pyrophosphorylase were expressed in ... variants of the murine fucokinase genes were expressed in COS-7 cells in frame with a 10-amino acid E2Tag present in the pQM vector The molecular masses of fucokinase proteins were determined by ... assayed in regard to fucokinase activity The specific enzyme activity of the human L-fucokinase was the same level, 12.3 pmolÆmg)1Æh)1, as the activity of the shorter mouse splice variant and the...
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Báo cáo khoa học: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt
... of CoA or acetyl-CoA The results are presented as the percentage of activity in the presence of the effectors in relation to the activity measured in the absence of the metabolites for each of ... than the other compounds (Fig 6) In this way, amino acid residues of different segments of NADP-ME2 swapped in the construction of the chimeras are involved in the fumarate activation of the pyruvate ... the percentage of activity in the presence of the effectors in relation to the activity measured in the absence of the metabolites, for each of the respective enzyme constructs The assays were...
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Báo cáo khoa học " Lentinula edodes enhances the biocontrol activity of Cryptococcus laurentii against Penicillium expansum contamination and patulin production in apple fruits " ppt
... strategy of biocontrol using agents capable of resisting or inhibiting the oxidants present in the wound This strategy involves boosting the biocontrol activity of C laurentii by complementing it ... extract The role of LF23 extract consists of enhancing the antioxidant enzyme activity of the yeast colonising the apple wounds and controlling patulin biosynthesis by promoting the antioxidant activity ... The activity of CAT and GPX was significantly higher during all the experiments in the mycelia treated with LF23, whereas the activity of SOD in the treated mycelia was not stimulated during the...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
... geometry of the hinge loop after ligation Also in this case no clashes were detected at Electrostatic interactions and antitumor RNases the dimer interface of the models or in the surroundings of the ... Nter are the C-terminal and N-terminal structural regions, respectively, of the two A and B monomers of the dimeric RNase The black circle between the monomers indicates the mass center of the dimer ... to outside interactions of the negatively charged glycerol-linked phosphate [23–25] In DMPS vesicles, the bonding of the serine carboxylate ion to the adjacent ammonium group renders the phosphate...
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Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... [39] is the fact that, in the absence of Vi (± drugs) labeling of the enzymes with the mutation in NBD2 is consistently lower than labeling of the enzymes with the equivalent mutation in NBD1 ... trypsin-sensitive region situated in the linker domain of Abcb1a Following trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation products of the two mutants R ⁄ Q ... reproducibility of our current assay; and would represent < 1% of the activity of the wild-type enzyme We then determined, by photoaffinity labeling, whether any of the mutations altered the apparent binding...
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Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf
... The fa value for KI was only 12% implying the low solvent accessibility of tryptophan residues of d2-crystallin in the native form ANS binding Fig Equilibrium unfolding of duck d-crystallin in ... the formation of dimers during the dissociation step (Fig 1) These results are in agreement with the double dimer structure of the tetrameric d2-crystallin The crystal structure of the protein ... is the dynamic quenching constant and fa was the fractional maximum accessible protein fluorescence ANS binding assay The exposed hydrophobic surfaces of the protein were assayed by incubating the...
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Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx
... membrane [30] The identication of proteins located on the extracellular surface and involved in the decrease in Eh would be of interest for increasing our understanding of the mechanisms involved ... attractive model for investigating new mechanisms involved in the reducing activity of microorganisms This was the aim of the present study Thiol groups in the reducing activity of L lactis 50 5.0 ... proteins in B subtilis such as BdbB, BdbC or CcdA may also be involved in the display of exofacial thiol groups and their role in decreasing the Eh can be implied Thiol groups in the reducing activity...
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Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt
... structure of the NOX1 gene and its splicing pathway Open boxes indicate exons Numbers in the box denote the size of each exon (bp) The size of the intron is indicated under the broken lines (bp) ... be involved in an angiotensin II-induced vascular hypertrophy [15], and in inducing the expression of monocyte chemoattractant protein-1 [16], which plays a key role in the development of atherosclerosis ... in the promoter region of the NOX1 gene, ATF-1 was assumed to indirectly regulate the expression of the NOX1 mRNA We therefore elucidated the role of ATF-1 in the MEF2B-dependent activation of...
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Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot
... chlorine and methyl functional groups at the 5-position Together, these results provide insight regarding the mechanism, the substrate binding site and the role of the metal cofactor in the enzyme ... state In the latter case, if the substrate coordination to the metal ion is not bidentate, interaction of the single metal ion in the active site with the substrate C2-carbonyl oxygen instead of the ... method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Laemmli UK (1970) Cleavage of structural proteins during the assembly...
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Báo cáo khoa học: The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane doc
... helix–helix packing interface involving the aminoacid residues on both sides of the insertion [40] In the other two mutants, the small amino-acid residues Gly15 and Gly19 were replaced by leucine residues ... and eG19L strains were incubated in the presence or absence of CuCl2 The control experiment (in the absence of CuCl2) was performed in the presence of NEM instead of CuCl2 Cross-linking conditions ... were incubated in the presence or absence of CuCl2 as described in the experimental procedure The control experiment (in the absence of CuCl2) was performed in the presence of NEM instead of CuCl2...
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The quality of financial assessment in the lending activity of Viet Nam commercial banks (Vietnam Joint Stock Commercial Bank for Industry and Trade)
... evaluation of the investment project involving lending activity of the commercial banks 1.4.2 The indicators to the quality of the financial evaluation of the investment project involving lending activity ... decide whether to len 1.3 The financial evaluation of the investment project involving lendingg activity of the 1.3.2.2 The content of the financial evaluation of the investment project of the commercial ... evaluation of the investment project involving lending activity of the commercial banks 1.4.1 The definition of the quality of the financial evaluation of the investment project - Check before lending...
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7Microbial Enzymes in the Biocontrol of Plant Pathogens and aEnzymes in the Environment: Activity, Ecology and Applications - Chapter 7PestsLeonid Chernin and Ilan ChetThe ppt
... inhibited in all chitin-containing fungi The degree of inhibition was proportional to the level of chitin in the cell wall of the target fungus Combining the two enzymes resulted in a synergistic increase ... ability B Lytic Enzymes Involved in the Biocontrol Activity of Other Fungi Besides Trichoderma spp., several other fungi exhibit the role of cell-wall -lytic enzymes in biocontrol activity (Table ... thuringiensis strains and comparing their activity against pests with that of the parental strain, (ii) by adding chitinolytic enzymes from other sources to B thuringiensis with the aim of increasing...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... the synthesis of amylopectin, it has been suggested that SSI is mainly involved in the synthesis of small chains of this fraction Furthermore, SSIV has been found recently to be involved in the ... the D2 domain and D(495–535) in the D3 domain, are involved in the interaction with CD, and that this interaction enhances the catalytic activity of the enzyme Our results show that the interaction ... interaction between the N- and C-terminal domains of SSIII in vitro Mapping of the CD-binding region in the N-terminal SBDs In order to identify the SBD region required for the SBD–CD interaction, we...
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Báo cáo Y học: The N-terminus of m5C-DNA methyltransferase Msp I is involved in its topoisomerase activity docx
... Characterisation of independent DNA and multiple Zn-binding domains at the N terminus of human DNA-(cytosine-5) methyltransferase: modulating the property of a DNA-binding domain by contiguous Zn-binding ... we indeed observed a protein that had retained the specificity and kinetic properties of a MTase [26] but had lost its topoisomerase activity, indicating the role of the N-terminal portion in ... (Fig 5) Deletion of 85 residues of the N-terminal region does not affect either the MTase activity or specific DNA binding in M.EcoRII, which possesses one of the largest N-terminal sequences (98...
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báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt
... primordia in (A), showing staining at the base of trichomes (C) Detail of an emerging later root (D) 15-day-old pVvMYB60:GUS seedling (E) Magnification of leaf primordia in (D), showing staining of ... conditions, the lines expressing the VvMYB60 gene displayed a rate of water loss indistinguishable from the one observed in the wildtype, thus demonstrating complete rescue of the stomatal defects of the ... MYB60 proteins and other closely related proteins from subgroup 1: a distinctive PHEEG signature in the MYB domain, located in the loop connecting the R2 and R3 repeats, and the lack of the first...
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Risks Involved in the Use of Herbal Products
... aspirin or other NSAIDs may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in blood sugar levels (hypoglycemia) Ginseng may intensify ... making it less effective Ginger taken with anticoagulants may increase the risk of bleeding Ginkgo taken with anticoagulants or with aspirin or other NSAIDs may increase the risk of bleeding Ginkgo ... from their ability to inhibit phase I and induce phase II carcinogen metabolizing enzymes that initiate carcinogenesis They also inhibit the promotion stage of carcinogenesis by inhibiting oxygen...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
... aggregation of some proteins by acting on the unfolded state of the protein and increasing the reversibility of unfolding [37] Arginine had no effect on the stability of the protein’s native ... a change in the number of fibrils formed in the presence of these additives In the presence of aB-crystallin (1.0 : 0.2 w ⁄ w ratio of a-synucleinA53T : aB-crystallin), the increase in ThT fluorescence ... on the overall activity of chaperone proteins Of the target proteins tested, Arg-HCl was found to specifically increase the activity of aB-crystallin against DTT-induced precipitation of insulin...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... for the binding of CP12 The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes observed for the binding of the substrate when the wild-type recombinant ... molecular modelling of all mutant GAPDHs indicate no changes of the global structure of the mutants To test whether the interactions of these mutants with the other partners of the GAPDHCP12PRK ... partners of this complex These mutageneses are a rst step toward the understanding of proteinprotein interactions in the GAPDHCP12PRK complex and the nature of the physico-chemical forces involved in...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... ThDP-dependent enzymes Substrate binding site and catalytic residues The active site cavity in IPDC extends from the thiazolium ring of the cofactor to the surface of the protein The entrance of the active ... formed by the PYR domains from one subunit and the PP domains of the other subunit within the dimer ThDP adopts the V-conformation [30,31] and is completely buried in the cofactor binding cleft...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... additional genes encoding other proteins implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most ... be implicated in the biosynthesis of the aminonucleoside moiety of puromycin [6–8] Interestingly, their organization is identical to that of the pur cluster, with the exception of pur6, which ... pertain to it Further sequencing of the ata cluster could provide an insight into the biosynthetic pathway of the other moieties of A201A and provide probes which may be useful to identify genes of...
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