lysine arginine histidine and glutamine
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... (E52) and arginine (R105), thought to stabilize the transition state and holding E52 in position during catalysis; and (b) investigate the amino acid residues involved in substrate binding (Y70 and ... residues: Y70 and Q81 Y70W showed an increase in Km values for both dThd and dCyd, of approximately 200- and 100-fold, respectively The kcat values decreased by approximately fivefold with dThd and twofold ... R-factor and Rfree were 23.2 and 24.2%, respectively (Table 3) Most of the protein could be found in the electron-density map, with the exception of residues 1–11 and 210–230 The loop connecting a9 and...
- 10
- 504
- 0
Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... genes RSc0337 and RSc1501 TH activity was determined at pH and 0.05% SDS, and DO activity at pH and 0.02% SDS genes, which was opposite to that expected from the blast homologies and designated ... 2-mercaptoethanol and 9% SDS) and heated at 95 °C for before application Electrophoresis was run at 20 °C and a constant current of 15 mA for 20 and 30 mA for 90 Protein bands were visualized ... respectively, p-quinones and dimeric quinonic structures (Fig 1) [7] Tyrosinases are responsible for vertebrate cutaneous pigmentation, browning of fruits and vegetables, and morphogenesis and fruiting body...
- 14
- 849
- 0
Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... exponential growth phase, and the cells were then washed and resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free ... ice, and the mixture was used as an analyte in SPR analysis AMP and glutamine are known to be the most effective inhibitors of GS [9] Figure 5B shows the effect of the feedback inhibitors AMP and ... inhibitors of GS being glutamine and AMP [9] Mutations in TnrA that result in constitutive expression of the TnrA-activated amtB promoter all lie within the C-terminal region of TnrA, and impair the...
- 11
- 596
- 0
Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... inhibition of NR and GS (Eur J Biochem 270) 1359 Fig Co-purification of glutamine synthetase and nitrate reductase inhibitor proteins (A) Activity of glutamine synthetase (h) and nitrate reductase ... and methods) and run on SDS/PAGE and stained with Coomassie blue Molecular mass standards (in kDa) are phosphorylase (97), bovine serum albumin (66), ovalbumin (43), carbonic anhydrase (30) and ... AMP from NADH, ATP and FAD by purified NR/GS inhibitor in a 100-lL incubation at 30 °C for 10 min, using the amounts and concentrations of ATP and NADH used in standard GS and NR assays, respectively...
- 7
- 457
- 0
Báo cáo Y học: A b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces noursei pot
... protein and b -lysine, the b -lysine binding protein was charged with radioactive b -lysine and the covalent enzyme±substrate complex was subjected to performic acid oxidation The radioactive b -lysine ... radioactive b -lysine in the presence of NpsA and ATP and subjected to SDS/PAGE Figure shows that the 70-kDa band was speci®cally labeled with b -lysine This reaction was ATP-dependent and also dependent ... presence of b -lysine The b -lysine binding protein was assayed in a coupled assay with the b -lysine activating enzyme The assay contained 1±2 pkatal of b -lysine activating enzyme, 0.1 mM b -lysine, 0.1...
- 11
- 559
- 0
báo cáo khoa học: "Effect of arginase II on L-arginine depletion and cell growth in murine cell lines of renal cell carcinoma" pptx
... with methanol and derivatization with OPA for (A) L -arginine and (B) L-ornithine and (C) L -glutamine Standards of L -arginine, L-ornithine and Lglutamine in methanol were run with each experiment ... inhibition, L -arginine depletion and L-ornithine production when nor-NOHA was added to cultures with CL-2 and Renca cells (data not shown) Figure L -arginine, L-ornithine and L -glutamine levels L -arginine, ... L-ornithine and L -glutamine levels Tissue culture supernatants from CL-2, CL-19, and Renca cells were collected at 24, 48, and 72 hours They were analyzed by HPLC after deproteinization with methanol and...
- 10
- 388
- 0
Báo cáo y học: "Comparison of cardiac, hepatic, and renal effects of arginine vasopressin and noradrenaline during porcine fecal peritonitis: a randomized controlled trial" potx
... to 0.72) μg/kg/min) Tables and and Figures and summarize the data on systemic hemodynamics and left heart function (Table 1), as well as O2 exchange, acid-base status, and metabolism (Table 2) ... collection RG, BH, and MG were responsible for the data analysis AS and PM provided the histomorphology and immunohistochemistry findings and the analysis of these data JV and UW were responsible ... flow and perfusion pressure [27] Consequently, the use of vasopressin is still cautioned in patients with heart and/ or peripheral vascular disease [2,3,5], and the multicenter Vasopressin and...
- 11
- 368
- 0
Molecular biology of glutamate dehydrogenase and glutamine synthetase in two air breathing teleosts
... (Cooper and Plum, 1987; Kanamori and Ross, 1995) Functional roles of glutamine synthetase and glutamine in nitrogen metabolism The catalytic formation of glutamine from glutamate and NH4+ involves glutamine ... As glutamine synthesis involves glutamate dehydrogenase (Gdh) and glutamine synthetase (Gs), this study was undertaken to examine the molecular biology of Gdh and Gs in M anguillicaudatus and ... anguillicaudatus and the liver, intestine and brain of Monopterus albus.………………………………………………………… … 36 Table Gene specific primers designed to amplify and sequence glutamate dehydrogenase (gdh) and glutamine...
- 220
- 318
- 0
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... amino acid transporter-2, and NOS in activated murine microglial cells (19) and co-induction of inducible NOS and arginine recycling enzymes in cytokine-stimulated PC12 cells and high output production ... continuous supply of arginine at the site of synthesis Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circulating arginine and (b) recycling ... were removed and killed by decapitation The brains were quickly removed and the different regions (CC, CB and BS) were separated according to the procedure described by Sadasivudu and Lajtha (22)...
- 8
- 622
- 0
Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... little contamination from xylem, and vice versa Glx (glutamine and glutamate: ve carbon amide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be ... Table Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and leaves in the light and dark The amino acid composition in ... glutamine and glutamate, as a result of a decline in GS2 and the induction of the a-GDH subunit (for a review, see [10]) However, the role of GDH is controversial [1114], and the regulation and...
- 14
- 566
- 0
Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... parasites after days and 2, 4, and 24 weeks after transfection Lanes 1, 3, 5, 7, 9, 11 and 13 show the PCR products obtained using the primer pair T7 and RIB Lanes 2, 4, 6, 8, 10, 12 and 14 show the ... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and ... after electroporation Hybridization bands with ADC- and rRNA-specific probes are shown in (A) and (B), respectively (C) Relative intensities of ADC mRNA bands normalized to the rRNA loading controls...
- 10
- 570
- 0
Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... Histidines and lysine in KAS I ⁄ KAS II catalysis P von Wettstein-Knowles et al Scheme group because of the cysteine and two histidine activesite residues [1–3] ... (b) two histidines (H298 and H333), promoting decarboxylation of malonyl and H333 also playing a role in the condensation reaction and for the pKa value of the nucleophile [7,8]; (c) a lysine ... cation ligands are main-chain oxygen atoms, and three are the side-chain oxygen atoms of N296, E342 and S387 The glutamic acid and asparagine residues are conserved among known KAS I and KAS II...
- 16
- 450
- 0
Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... Hoff analysis and those determined by uorescence and DSC (see before) Determination of the Th and Ts The values of the temperatures where the enthalpy and entropy are zero, Th and TsÂ, respectively, ... respectively; and at 318 K, 33 kcalặmol)1 and 4.4 3.9 kcalặmol)1ặM)1, respectively; (B) at 293 K, 3.7 3.3 calặmol)1ặK)1 and 0.7 calặ mol)1ặK)1ặM)1, respectively; and, at 318 K, 98 11 calặmol)1ặK)1 and ... of 3500 Da Standard suppliers were used for all other chemicals Water was deionized and puried using a Millipore system Urea and Gdm Cl stock solutions were prepared gravimetrically and ltered...
- 17
- 612
- 0
Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx
... heated at 98 °C for 10 and residual activity (A,C) and amount of aggregates (B,D) were determined Continuous, dotted, and broken lines show the fitted curves to no additives, Arg, and ArgEE with sigmoidal ... order to understand the importance of amphiphilicity, titration curves of Arg and ArgEE were compared (Fig 5A) The pKa of amino groups on Arg and ArgEE were determined as pH 9.2 and 7.4, respectively ... concentration of soluble protein (d) were determined and plotted The continuous and broken lines show the theoretical curves fitted to the closed and open circles with single exponential equations...
- 6
- 482
- 0
Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx
... Barrett and C Robinson between the TM spans of TatA and TatB, and between the amphipathic regions Finally, we have re-examined the numerous mutations made previously in TatA, B and C and we present ... it was previously found that changing two arginines (residues 37 and 40) to asparagine (TatB ⁄ 2R > N), or three lysines (residues 65, 67 and 68) to glutamine (TatB ⁄ 3K > Q) had little effect ... efficiently export TorA-GFP, including the E8Q and L63A mutants that exhibited no signal with the Streptag II immunoblots for TatC in Fig and 41) to glutamine, and in a second mutant we additionally...
- 15
- 532
- 0
Tài liệu Báo cáo khoa học: The swinging movement of the distal histidine residue and the autoxidation reaction for midge larval hemoglobins docx
... myoglobin-ethylisocyanide: histidine as a swinging door for ligand entry J Mol Biol 207, 459463 Tian, W.D., Sage, J.T & Champion, P.M (1993) Investigations of ligand association and dissociation rates in the ễopenế and ... into two approximately equal groups; one (VIA, VIB, VII, VIII and IX) having a distal histidine and the other (IA, IB, II, III, IV and V) lacking in it [1] We have therefore carried out a matrix ... stability of the bound dioxygen Among those components, hemoglobins V and VII are the major ones in each different group, and make up 15% and 30%, respectively, of the total hemoglobin concentration in...
- 10
- 443
- 0
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... Q69G and A141Q, both single and double mutations, and a comparison with data on equivalent mutations in MnSOD (G77Q and Q146A), which change in vivo metal selectivity, specific activity and thermal ... a reduction in selectivity in the order: no glutamine > one glutamine[ wt] > two glutamines > one glutamine (the double mutants) Thus the presence and position of this residue affects in vivo, ... three His metal ligands (H26, H81 and H171) and gateway residues H30 and H34 A further gateway residue, H31, is involved in FeSOD but not in MnSOD Only in MnSOD is the active site glutamine (Q146)...
- 12
- 740
- 0
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... 4773 glutamine ! glutamate ỵ NH3 ẵ8 and ATP ! ADP ỵ Pi Isothermal titration calorimetry (ITC) assay of glutaminase activity and CTP synthesis CTP synthase at concentrations between 0.029 and 1.16 ... yeast [11] and mammalian [12,13] enzymes that all require the presence of UTP and/ or ATP to form tetramers Therefore, the L lactis CTP synthase is an attractive candidate for mechanistic and structurefunction ... under these conditions before and after injection of glutamine and prior to kinetic experiments, it will not interfere with the measurements as this hydrolysis is slow and steady-state will prevail...
- 8
- 698
- 0
Báo cáo khoa học: Inactivating pentapeptide insertions in the fission yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine finger motif docx
... b-strand b4 in the five-strand parallel b sheet that comprises the core of domain I (Figs 2A and 3A) The b4 strand is the central strand in the sheet [9]; it is likely that disruption of this strand ... K12), between a1 and a2 (K32), between a2 and b1 (K22, K23 and K3), between b2 and a4 (K11) and at the N-terminal end of b3 (F46, F49) Two domain I deletion constructs (K33DK27 and K33D22) were ... entry 1SXJ) this domain comprises 11 a-helical segments and b strands [9] Here the helices are designated a1–a3, a3¢ and a4–a10 and the strands b1–b5 (Fig 2A) Note that in PDB entry 1SXJ, the a3–a3¢...
- 11
- 502
- 0
Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25