... by DNA sequencing. The design ofthe mutants was intended to preserve the amino acid character and to modify only the relativevolume of their R groups. The expression ofthe FNR mutants as soluble ... phe-nol as the tyrosine R group. This system has been usedpreviously to analyze the geometry ofthe tyrosinestacked on the si-face ofthe flavin in FNRs [12]. The single point energies ofthe flavin–tyrosine ... in the protein stability of our mutants. There-fore, these conclusions strengthen our hypothesis that the catalytic efficiencies ofthe recombinant enzymesused in our study were related to the...
... found with otherdissociated capsid proteins, the lack of elution of the proteins can be explained by non-specific binding of the denatured coat proteins to the gel [34,35]. The elution of some D11N ... Figure 5C shows that the spectra of the sample subjected to compression and decompression aresimilar to the non-treated sample, even in the case of the M88V mutant. The elution ofthe samples after ... promote significant binding of bis-ANS to any of the particles, with the exception of T45S, which bound asmall amount of bis-ANS, increasing, by twofold, the emission ofthe probe (Fig. 7B). M88V...
... disagreement about the intendedness (at time of conception) of recent births, with the father’s attitudes based on the mother’s reports of his attitude. A forthcoming report will describe fathers’ attitudes ... attitudes toward the intendedness of pregnancies based on their own reports (13). + Of all births in the 5 years before interview, 55 percent were intended by both the mother and the father (as ... (tables 97,98) The NSFG is one ofthe few reliable sources of nationally representative data on the use of infertility services. Earlier reports have documented the rise in use of these services...
... initiation of apoptosis through the mitochondrial pathway. The key event in the mito-chondrial pathway is the release of proapoptotic fac-tors from the mitochondrial intermembrane space into the cytosol, ... release of cytochrome c and second mitochondria-derivedactivator of caspase (Smac ⁄ DIABLO) allows for the formation ofthe apoptosome, a complex that enables the activation of caspases within the ... The Authors Journal compilation ª 2010 FEBS 1325 The ubiquitin ligase Itch mediates the antiapoptoticactivity of epidermal growth factor by promoting the ubiquitylation and degradation of the...
... notunderstand the “rules ofthe game” the way the United States does.That is one interpretation of both the crude Chinese nuclear threatsover the Taiwan crisis and Chinese views ofthe U.S. bombing of the Chinese ... confronta-tion between the Soviet Union and the United States was the centralreality in world politics. With the end ofthe Cold War and the disso-lution ofthe Soviet Union, the world continues ... result of agreementswith the United States and unilateral actions in the aftermath of the Cold War, and partly because ofthe state ofthe Russian economy.Even if Russia had not ratified the Strategic...
... contact with the carbonyl oxygen of Gly255.These movements away from the oxidized cysteineincrease the space inside the P-loop to accommodate the large sulfonic acid group. The movements in the P-loop ... serve to provide room for the larger oxidizedcysteine. The key feature that dictates whether the P-loop moves upon oxidation ofthe catalytic cysteine is the ability ofthe conserved active site ... with Od2 ofthe cysteine sulfonic acid. In addi-tion to these contacts, there is a 1.82 A˚contactbetween Gly255 Caand Od2 ofthe oxidized cysteine.To further understand the structural...
... glycation, rather thanpurely the loss ofthe parent amino acid, which is the key factor in terms of receptor recognition [29]. Thereare no data available on the extent of Lys (and otheramino ... receptor-blockingexperiments. The rates of uptake of both the lipid andprotein components are not matched by the rate of cel-lular metabolism of these species, resulting in the accu-mulation of both unmodified ... andcontrol LDL, for 96 h. The use of LDL-free mediumduring the chase period allows the turnover of preaccu-mulated protein to be studied in the absence of furthercellular uptake. In these studies, cell...
... by the replacement of Fe by Zn in the rubredoxin given the identical structures ofthe twoprotein forms [38,39]. Therefore, at the concentrationsused in the NMR experiments over 90% of the cytochrome ... ofthe haem methyl groups in the fully reduced stage 0 are not plotted because they are unaffected by the pH. The solid lines represent the best fit ofthe shifts for DvHc3:Pi to the model of ... is bound to the rubredoxin and the effectobserved in the signals ofthe haem methyls of DvHc3:ZnRb complex vs. the results in the isolatedcytochrome is close to the full effect of complex...
... productive binding of steroid substrates:in the first, a (small) sub population of enzyme bindsdirectly the steroid in the reactive conformation, with the remainder ofthe enzyme binding the unreactiveconformation. ... grossly perturb the overall framework ofthe enzyme or the active site. The active sites of the enzymes are shown in stick format with the sigmaA weigh-ted 2F0-Fcdensity for the Y186F mutant ... bond inclose proximity ofthe N5 atom. A putative flippingmotion ofthe progesterone molecule along an axisparallel to the FMN plane aligns the C1-C2 with the plane ofthe isoalloxazine ring...
... ofthe structure of AII in solution with the X-raystructure of AII complexed to the Fab131 mAb. In Fig. 7A, asequence alignment is represented ofthe backbone of the conformational ensemble of ... alignment ofthe fragment 4–7 ofthe backbone of the 13 ensemble solution structures of AII (brown colour) superimposedon the X-ray structure (dark blue colour) and (B) structure alignment of the ... of the fragment 4–7 ofthe 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII.Fig. 6. Structure of a representative folded conformer of AII showing the van der Waals...
... ShoppersElectronically or the Old Fashioned Way Market newsletters are a great method of reminding your shoppers of why they love the market. The trend in this type of communication is the e-newsletter ... Marketing the MarketKatherine Kelly and Joan Vibert, vendors at Kansas City’s Brookside Farmers’ Market, offer the following possibilities for vendors to contribute to the success ofthe market ... vendors may not achieve their potential.Through marketing the market, many ofthe preced-ing relationships can been strengthened. When vendors make the effort to market the market as a whole...
... counteracts the effect ofthe mutations, stabilizing the dimericform. The stabilizing effect is even more pronounced ifl-serine binds to the cofactor at the active site, as isevident in the case ofthe ... region in this group of enzymes, interacting with the phosphate moiety of PLP. Therefore, the capability of the third cluster of CHCs to confer stability to the polypeptide loops ofthe active site ... structure.Discussion The decrease of hydrophobic contact area determinedby the mutations in the third cluster of CHCs caused ashift ofthe equilibrium between dimeric and monomericforms of eSHMT in favor of the...
... at the last base, G to A, abolished the interaction of HIPPI, so the last motif would notinteract with HIPPI. The other two motifs might be the target of HIPPI. In the upstream sequence of the Table ... positions ofthe motifs are indicatedby distance from the transcription start site (TSS). The position upstream ofthe TSS of any gene is denotedby ‘–’ followed by the distance from the TSS. In the caspase-8 ... confirmed the in vitro resultthat mutation ofthe motif abolished the binding of HIPPI to the specific sequence ofthe caspase-1 geneupstream sequence, and the increased expression of caspase-1...
... in the presence of KCl.Considering also the 3D structure of CDase I-5, the analysis ofthe quaternary state of CDase I-5 revealedFig. 8. Fluorescence spectra of CDase I-5. (A) - - - -, the ... the adjacentmonomer, of which G538 forms a hydrogen bond to the carbonyl oxygen of M51. Two residues at the N-terminus (H49 and H89) of a subunit were locatedclose to the C-domain ofthe other ... either in the secondarystructure ofthe protein according to far-UV CDdata or in the size ofthe protein determined by size-exclusion HPLC. They suggested that the changemight occur either...
... is the information gathered for the own use ofthe researcher and never gather before. The advantage ofthe primary data compared to secondary data are the information gathered is best suit the ... buy the company‟s goods and products, some of them go to tell the others about the bad things they suffer and convince them not to support the company any more. So, in order not to loose the ... shopping. On the other hand, if the consumers dissatisfy with the goods and services they buy, they would set a defensive thought against them and view the company in a bad image, some of them stop...